The mycelium growth and sporulation in different temperature, pH value and light conditions were detected by using the crossing and haemocytometer method. The toxicity of five fungicides to Fusarium oxysporum was tested by mycelia growth method, so as to provide the reference for prevention and control against F. oxysporum. The optimum temperature and pH value of F. oxysporum to grow and spore were 28 degrees C and 6-7. Alternating light and darkness promoted growth and sporulation of bacterial colony. As for five fungicides, the EC50 of tebuconazole was 10.02 mg x L(-1), 92.50 times as much as carbendazim. The EC50 of myclobutanil and Fuxing was 91.23, 96.68 mg x L(-1), respectively. Tebuconazole showed the most tremendous inhibitory effect and control efficiency on F. oxysporum.
Fungicides, Industrial ; pharmacology ; Fusarium ; drug effects ; growth & development ; Mycelium ; drug effects ; growth & development ; Orchidaceae ; microbiology ; Plant Diseases ; microbiology ; Spores, Fungal ; drug effects ; growth & development

Traditional Chinese medicinal resource survey method is time-consuming, strenuous, and having great human influence, the precision is not high enough. This paper, by using spatial information technology, carries on spatial sampling survey for wild medicinal plants resource for generous species to arrange the quadrat scientifically and estimate the suitable area, reserve precisely of medicinal plants. It not only improves the survey precision, but reduces the workload of field survey and provides scientific basis for survey method of pilot work on the fourth national traditional Chinese medicinal resource census.
Biodiversity ; Conservation of Natural Resources ; Ecosystem ; Humans ; Plants, Medicinal ; Spatial Analysis

According to the process of the technical specification (TS) design for the fourth national survey of the Chinese materia medica resources (CMMR), we analyzed the assignment and objectives of the national survey and pointed out that the differences about CMMR management around China, the distribution of CMMR and their habitat, the economic and technological level, and even enthusiasm and initiative of the staff, etc. are the most difficult points for TS design. And we adopt the principle of combination of the mandatory and flexibility in TS design. We fixed the key points which would affect the quality of national survey first, then proposed the framework of TS which including 3 parts of organization and 11 parts of technique itself. The framework will serve and lead the TS preparation, which will not only provide an action standard to the national survey but will also have a profound influence to the popularization and application of the survey technology of CMMR. [Key words]
Chemistry, Pharmaceutical ; legislation & jurisprudence ; manpower ; methods ; standards ; China ; Conservation of Natural Resources ; Data Collection ; Drugs, Chinese Herbal ; chemistry ; standards ; Materia Medica ; chemistry ; standards ; Quality Control

The planning of the fourth national survey on Chinese materia medica resources shall follow the requirement of "carrying out the national survey on Chinese materia medica resources, strengthening the construction of Chinese materia medica resources monitoring and information network" which is according to the document issued by the State Council. Based on the responsibilities of State Administration of Traditional Chinese Medicine which is "organizing the survey, promoting the resource-protection, keeping development and rational utilization", combined with the key problems that need to be solved in current medicinal industry, the related instructions issued by central leadership and the recommendations from NPC delegates, CPPCC members and experts, the planning shall make overall plans and top-level design for the new round of national survey on Chinese materia medica resources.
China ; Data Collection ; Databases, Pharmaceutical ; Drugs, Chinese Herbal ; supply & distribution ; Goals ; Materia Medica ; supply & distribution ; Research Design ; Social Control, Formal

OBJECTIVETo analyze the appropriate producing area of Cistanche tubulosa by mean of TCMGIS-I.

METHODThe TCMGIS-I (Suitability evaluation geographic information system of traditional Chinese medicine producing area) was used to analyze the appropriate producing area of C. tubulosa basing on the optimum ecological factors of Sorth Sinkiang municipality which is the traditional producing area of C. tubulosa.

RESULTThe suitable producing areas of C. tubulosa include 56 counties with 190952.1 km2 total areas in Sinkiang municipality, Neimongd municipality and Gansu province; Sinkiang municipality, Gansu and Qinghai provinces have the second-suitable producing areas of C. tubulosa, with 46 counties and 41217.37 km2 total areas; Sinkiang municipality, Inner Mongolia municipality, Gansu province and Ningxia municipality have the third-suitable producing areas of C. tubulosa with 56 counties and 421145.2 km2 total areas.

CONCLUSIONIt is important to analyze the appropriate producing area of C. tubulosa by means of TCMGIS-I. The results are useful for development of C. tubulosa transplanting and cultivation.

China ; Cistanche ; growth & development ; Geographic Information Systems ; Geography

According to National Census for Water, National Population Census, National Land and Resources Survey, and work experience of experimental measures for national Chinese materia medica resources(CMMR) survey,the national CMMR survey at the county level is the key point of whole survey, that includes organization and management, field survey, sorting data three key links. Organization and management works of national CMMR survey needs to finish four key contents, there are definite goals and tasks, practicable crew, preparation directory, and security assurance. Field survey works of the national CMMR survey needs to finish five key contents, there are preparation works for field survey, the choice of the key survey area (samples), fill in the questionnaire, video data collection, specimen and other physical collection. Sorting data works of the national CMMR survey needs to finish tree key contents, there are data, specimen and census results.
Chemistry, Pharmaceutical ; China ; Data Collection ; Materia Medica ; chemistry ; supply & distribution ; Medicine, Chinese Traditional

OBJECTIVETo analyze the appropriate producing area of Cistanche desertiola.

METHODThe TCMGIS-I (suitability evaluation geographic information system of traditional Chinese medicine producing area) was used to analyze the appropriate producing area of C. desertiola basing on the optimum ecological factors of Aalashan, Inner-Mongolia autonomous region and North Xinjiang au-tonomous region, the traditional producing areas of C. desertiola.

RESULTThe results showed that the suitable producing areas of C. desertiola included 159 counties of 5 autonomous regions and provinces, i.e. Xinjiang, Inner Mongolia, Ningxia autonomous regions, and Gansu as well as Qinghai provinces. The total area was 675 354.9 km2 and distributed mainly in Aalashan of Inner Mongolia, eastern part of North Xinjiang, northern part of Gansu and in the middle of Ningxia.

CONCLUSIONThe suitable producing areas based on TCMGIS-I covered all areas registered in the 3rd national investigation of Chinese traditional medicine resource. And the results were also corresponding to the traditional producing area of C. desertiola recorded in ancient literature and the successful cultivation areas nowadays. It suggested the rationality and reliability of TCMGIS-I.

Breeding ; China ; Cistanche ; growth & development ; Climate ; Conservation of Natural Resources ; Databases, Factual ; Ecosystem ; Geographic Information Systems ; Plants, Medicinal ; growth & development ; Soil

AIM:To investigate the effects of ubiquitin-specific peptidase 9, X-linked (USP9X) down-regula-tion on apoptosis and invasion ability in gastric carcinoma cells, and to explore its possible molecular mechanisms. METH-ODS:USP9X small interfering RNA (siRNA) and control siRNA were used to be transfected into gastric carcinoma AGS cells. The cells were divided into 3 groups, including untreated AGS group, control siRNA group and USP9X siRNA group. The expression of USP9X at mRNA and protein levels in the AGS cells with different treatments was determined by real-time PCR and Western blot. The cell viability was analyzed by CCK-8 assay. Flow cytometry and Boyden chamber were employed to examine the apoptosis and invasion ability of the AGS cells. RESULTS:USP9X siRNA significantly down-regulated the expression of USP9X at mRNA and protein levels in the AGS cells. Down-regulation of USP9X markedly induced apoptosis and reduced invasion ability of the gastric carcinoma AGS cells. Notably, down-regulation of USP9X sig-nificantly reduced the protein expression of Mcl-1 and MMP-2, but markedly increased the protein level of Bax. CON-CLUSION:USP9X may be a key regulator for apoptosis and invasion in gastric carcinoma.

Sustainable utilization and bio-diversity protection of traditional Chinese medicine (TCM) have been a hotspot of the TCM study at present, in which the choice of appropriate method is one of the primary problems confronted. This paper described the technical system, equipment and image processing of low altitude remote sensing, and analyzed its future application in Chinese herb medicinal sustainable utilization.
Altitude ; Conservation of Natural Resources ; methods ; Drugs, Chinese Herbal ; Image Processing, Computer-Assisted ; Medicine, Chinese Traditional ; Robotics ; instrumentation ; methods

OBJECTIVETo supply the scientific data for the choice of medicinal plants' producing area through analyzing the suitable area for Panax quinquefolium' growth.

METHODTCMGIS-I (Suitability evaluation geographic information system of traditional Chinese medicine producing area) was developed and used to analyze the suitable producing area of P. quinquefolium Wisconsin state of USA, one of the origin producing area of the geo-authentic crude drugs, was selected as the standard analytical area and some key factors related to plant growth such as average temperature, altitude, soil type, precipitation were chosen to be considered.

RESULTThe result showed that the suitable area for P. quinquefolium' growth in China is similar to the present status. It concentrates in the northeast and the north of China, and part of Shanxi province is also suitable but the field area is small.

CONCLUSIONThe development of P. quinquefolium in China should concentrate in the northeast and the north of China. The TCMGIS-I is valuable to the analysis of suitable producing area and introduction of medicinal plant.

China ; Climate ; Drugs, Chinese Herbal ; Feasibility Studies ; Panax ; growth & development ; Soil ; Wisconsin