BACKGROUNDInhibition of the key costimulatory signals results in T cell anergy, indicating the alloantigen-specific immunologic unresponsiveness. In this study, the effect of blockage of costimulatory signal CD(86) on murine abortion-prone model was studied.

METHODSThirty CBA/J female mice cohabitated with DBA/2 male or BALB/c male mice were investigated. CBA/J x DBA/2 matings were used as the abortion-prone model, and CBA/J x BALB/c matings were used as the normal pregnant model. The abortion-prone models were divided into experimental and control groups, and the normal pregnant models were set as a normal group (10 mice in each group). The mice in the experimental group were treated with anti-mouse CD(86) monoclonal antibody (mAb) (100 microg) on day 4.5 of gestation, while the controls received irrelevant-isotype matched rat IgG(2b). As for the normal group, nothing was given to the mice. The mice were killed on day 13.5 of gestation, embryo resorption rate and the expression of transforming growth factor beta(1) (TGF-beta(1)), plasminogen activator inhibitor 1 (PAI-1), and matrix metalloproteinase 9 (MMP-9) were detected. Then the data were analyzed by Chi-square test and Fisher's exact test.

RESULTSThe embryo resorption rate in the experimental (8.2%) and normal groups (7.7%) was significantly lower than that of the control (23.5%, P < 0.05). No significant difference was detected between the experimental and normal groups (P > 0.05). The positive expression rates of TGF-beta(1) and PAI-1 proteins in the experimental and normal groups were significantly higher than those in the control group (P < 0.05). The positive expression rate of MMP-9 protein in the experimental and normal groups was significantly lower than that in the control group (P < 0.05). No significant difference in the positive expression rates of the three proteins was detected between the experimental and normal groups (P > 0.05).

CONCLUSIONSBlockage of costimulatory signal CD(86) at early pregnancy can treat uncertain recurrent spontaneous abortion by stimulating the expression of TGF-beta(1), MMP-9 and PAI-1 and reducing the embryo resorption rate.

Abortion, Habitual ; therapy ; Animals ; Antibodies, Monoclonal ; therapeutic use ; B7-2 Antigen ; immunology ; physiology ; Embryo Loss ; prevention & control ; Female ; Immunohistochemistry ; Male ; Matrix Metalloproteinase 9 ; analysis ; Mice ; Mice, Inbred Strains ; Plasminogen Activator Inhibitor 1 ; analysis ; Pregnancy ; Signal Transduction ; Transforming Growth Factor beta1 ; analysis

Objective To explore the common characters of sub-health and health risk factors among the public officials. Methods A total of 2776 public officials were tested using human energy monitor.The score differences of health risk factors and the differences of risk detection rates (moderate and above)between males and females were compared.Results The scores of 1 2 kinds of health risk factors by descending order were lack of energy(1.1 1 ±1.01 ),susceptible to fatigue (1.09 ±1.26),stuffy nose(0.95 ±1.59),chest tightness and shortness of breath (0.94 ±0.91 ),difficulty in falling asleep (0.89 ±1.59 ),lack of appetite (0.76 ±0.76 ),anxiety (0.75 ±1.1 6 ),oral inflammation (0.75 ±1.21 ), respiratory disorders (0.69 ±0.83),eye fatigue (0.61 ±0.72),backache and leg swelling (0.60 ±0.72),and stomach upset (0.48 ±0.65 ),respectively. The scores of women were significantly higher than those of men in 1 1 kinds of health risk factors(P<0.01 )except the score of anxiety (P>0.05 ).The risk detection rates (moderate and above)of women were significantly higher than that of men (P<0.01 )in the following 5 aspects:lack of energy,susceptible to fatigue, stuffy nose,mouth inflammation,and backache and leg swelling.Conclusion Chronic fatigue syndrome is the most common character of the sub-healthy public officials,and women are more prone to sub-health state.