ObjectiveTo investigate the relationship between the clinical type of attack and electroencephalogram (EEG) features of cerebral palsy (CP) children with epilepsy (EP).MethodsThe EEGs of 36 CP cases with EP were recorded under natural circumstances.Results20 cases (55.6%) had focal high amplitude spikes and wave complexes; 4 cases (11.1%) had typical hypsarrhythmia; 3 cases (8.3%) had varied hypsarrhythmia. Among them, the spikes and wave complexes of 9 cases were bilateral.ConclusionThe most of the clinical attack and EEG characters of CP children with EP are partial type.

Objective To explore the regularity of heat shock protein 70(HSP70)expression and its function in renal ischemia/reperfusion injury(IRI),and to reveal whether traditional Chinese medicine, Xuebijing injection(血必净注射液),induces HSP70 synthesis and has a protective effect on IRI.Methods One hundred and eight female Sprague-Dawley(SD)rats,(200?20)g in weight,were used in this experiment,and randomly divided into three groups(each n=36):control(A),model(B)and treatment group(C).The I/R model was established by clamping renal pedicles on both sides for 45 minutes to cause ischemia and then reperfusion was made.In group A,a similar model procedure was performed,but without ischemia.At 5-10 minutes before the IRI was performed,5 ml/kg of normal saline and 5 ml/kg of Xuebijing injection was injected through the femoral vein in group B and C respectively.In B and C groups,according to the durations of reperfusion for 0,6,12,24,36 and 48 hours,the rats were subdivided into six subgroups (each n=6).At the end of each time point,blood and renal tissue were collected to measure blood creatinine (SCr)and urea nitrogen(BUN),and Western blotting method was used to examine the expression of HSP70. In another kidney,renal tissue was obtained for hematoxylin and eosin(HE)staining pathological and immunohistochemical examinations.Results In B and C groups,the SCr and BUN levels at 6 hours after I/R were significantly higher than those in the A group,and those in the C group were lower than those in the B group(P

Objective To establish an ultra performance liquid chromatography method for the determination of 7 kinds of antibiotics(minocycline hydrochloride,ox tetracycline,tetracycline hydrochloride,chlortetracycline hydrochloride,doxycycline hydrochloride,chloramphenicol,metronidazole) in anti-acne cosmetics.Methods Seven kinds of antibiotics were separated on a Waters UPLC BEH C18 column by gradient elution using A(methanol/acetonitrile = 1/2) and B(1% formic acid aqueous solution) as the mobile phase at a flow rate of 0.2 ml/min,with the detection wavelength of 268 nm.Results The detection limits were 0.3-0.5 ng,the calibration curves were linear in the range of 2-100 ng(1-50 mg/L),the precision was not more than 4.8% and the rate of recovery were 82.5%-105.6%.Conclusion The method established in the present paper is rapid,sensitive and suitable for the determination of 7 kinds of antibiotics in anti-acne cosmetics.

Objective To evaluate the significance of dual-color interphase fluorescence in situ hybridization (FISH) in the engraftment estimation and minimal residual disease (MRD) monitoring after sex-mismatched allogeneic hematopoietic stem cell transplantation (allo-HSCT).Method The fluorescence signal of samples from 35 cases in different periods after sex-mismatched allo-HSCT were detected by interphase FISH using chromosome enumeration probes (CEP) X and Y. Results All of 35 patients had been determined to obtain engraftment after allo-HSCT. When the disease relapsed,FISH showed that the percentage of donor chromosomes was decreased and when the disease got remission,the percentage of donor chromosomes increased. When conventional cytogenetics showed 100 % XX or 100 % XY,FISH showed different percentage of host chromosomes.Conclusions The test of dual-color interphase FISH is reliably sensitive and simple for engraftment evaluation and MRD monitoring post HSCT. It is a good complement method to cell morphology and traditional karyotype analysis.

Objective To evaluate the relationship of intraerythrocytic calcium(Ca~(2+)),serum cardiac troponin I(cTnI) and contractile and diastolic dysfunction in infants with pneumonia complicated with heart failure(HF),and discuss the role of Ca~(2+) and cTnI.(Met)-hods One hundred and thirty-three infants with pneumonia complicated with HF and 30 healthy children were studied.Intraerythrocytic calcium,serum cTnI and ejection fraction(EF),fractional shortening(FS) and E/A were measured by color Doppler ultrasoundraphy.Results Intraerythrocytic calcium,serum cTnI and EF,FS and E/A in two groups had difference.With the disease deteriorating,the values of Ca~(2+) and cTnI increased,and EF,FS,E/A decreased gradually.There were negative relations between Ca~(2+),cTnI and EF,FS,E/A.Conclusions Ca~(2+),cTnI are concerned with the development of HF.In clinical experiments,the contractile function and diastolic function of heart can be judged by the levels of intraerythrocytic calcium and serum cTnI.

Objective: To screen candidate genes involved in the terpenoid biosynthetic pathway of Tussilago farfara. Methods: The transcriptome of buds and leaves of wild T. farfara were respectively sequenced using the Illumina HiSeq 2500 high-throughput sequencing platform. The clean reads were de novo assembled by Trinity software, and the assembled sequences then followed by a series of bioinformatics analysis such as gene function annotation and differential expression gene. According to sequence annotation and differentially expressed genes analysis, the key enzyme genes related to the terpenoid biosynthesis were identified. Results: After high through-put sequencing, a total of 39 912 371 clean reads were obtained (SRA accession: SRR9113366, SRR9113367). The clean reads were then assembled into 91 118 unigenes. A total of 55 830 unigenes were annotated by a similarity search against NR, Swiss-Port, GO, COG, KEGG five public databases. Base on KEGG annotation and differentially expressed genes, totally 129 catalytic enzyme genes referring to the terpenoid biosynthesis were identified, including 91 terpenoid backbone biosynthesis genes, 32 terpene synthases, and 6 cytochrome P450 (CYP450) genes. Among them, 25 genes were differentially expressed. The expression of four enzyme genes in MVA pathway in leaves were higher than that in buds, while the five enzyme genes in MEP pathway were lower in leaves than that in buds. In addition, 10 genes were highly expressed in leaves, and nine genes were highly expressed in buds. According to the high expression of differentially expressed HMGR, TPS, AS, CYP450 genes in buds, it was speculated that these genes may be related to the high content of terpenoids in flower buds. Conclusion: This work obtained candidate key enzyme genes that may be involved in the biosynthesis of terpenoid by transcriptome sequencing. The results laid a foundation for further elucidating the molecular mechanism of terpenoid biosynthetic pathway in T. farfara.

Objective: To investigate the technical procedure, clinical efficacy and safety of extrathoracic and transhepatic CT-guided radiofrequency ablation (CT-RFA) for liver cancer in hepatic dome area. Methods: A retrospective analysis was conducted in 50 patients who were diagnosed of primary liver cancer or metastatic liver cancer and received CT-RFA between September 2003 and October 2012. The total number of the malignant lesions in the hepatic dome area were 59. The detailed technical procedure, clinical efficacy and the safety as well as the factors related to local recurrence-free survival were anaylized and summarized. Results: The pathway for needle puncture of RT-RFA in treatment of the malignant lesions in the hepatic dome area was established by using a new "cube" model. The rates of technical success in RFA and complete necrosis were 94.92% (56/59) and 84.75% (50/59), respectively. The median local recurrence-free survival time of patients with completely necrotic lesions was 1 2.05 months, which for primary liver cancer and metastatic liver cancer were 14.23 and 8.07 months, respectively (P = 0.037). Three patients developed severe complications after RFA, and recovered after supportive treatment. The multivariable analysis revealed that the tumor size was an independent factor related to the local recurrence-free survival (P = 0.028). Conclusion: CT-RFA for the treatment of malignant lesions in the hepatic dome area is safe and effective. The tumor size is an independent risk factor related to the local recurrence-free survival. Copyright© 2014 by TUMOR.

BACKGROUNDConsidering the existence of a large number of liver cell degeneration and necrosis in fibrotic liver, liver function was damaged severely and could not effectively regenerate after partial hepatectomy (PHx). The aim of this study was to investigate whether decorin (DCN) could promote the liver regeneration after PHx in fibrotic mice.

METHODSForty mice (5-week-old, Balb/c) were injected with CCl4 intraperitoneally and liver fibrosis model was established after 5 weeks. The survival mice were randomly divided into two groups: control group and DCN group. Then, we performed 70% PHx on all these mice and injected DCN or phosphate-buffered saline plus normal saline (NS) to each group, respectively, after surgery. Liver body weight ratio (LBR), quantitative real-time polymerase chain reaction, and immunohistochemistry were used to analyze liver regeneration and fibrosis degree in both groups, and to find out whether exogenous protein DCN could promote the regeneration of fibrosis liver after PHx.

RESULTSExpressions of a-smooth muscle actin (SMA) mRNA and LBR had significant increases in the DCN group at postoperative Day 3 (POD 3, P < 0.05). The protein expressions of CD31, a-SMA, and tumor necrosis factor (TNF)-a were higher in the DCN group than those in the control group by immunohistochemistry at POD 3 (P < 0.05).

CONCLUSIONExogenous protein DCN could promote liver regeneration after PHx in fibrotic mice.

Animals ; Decorin ; therapeutic use ; Hepatectomy ; Immunohistochemistry ; Liver Cirrhosis ; drug therapy ; metabolism ; surgery ; Liver Regeneration ; drug effects ; Male ; Mice ; Mice, Inbred BALB C ; Platelet Endothelial Cell Adhesion Molecule-1 ; metabolism