Nanofat is a chylous fluid obtained by mechanical emulsification and filtration of adipose tissue, which can pass through 27 G needle and be used for wound treatment, scar repair, skin rejuvenation, etc. In recent years, some researchers have proposed modified procedures based on the traditional preparation procedures of nanofat. Meanwhile, there has been increasingly more attention on the mechanism of core components of nanofat to promote wound healing. This paper reviewed the recent literatures on the mechanisms and clinical applications of nanofat in wound healing improvement.

Nanofat is a chylous fluid obtained by mechanical emulsification and filtration of adipose tissue, which can pass through 27 G needle and be used for wound treatment, scar repair, skin rejuvenation, etc. In recent years, some researchers have proposed modified procedures based on the traditional preparation procedures of nanofat. Meanwhile, there has been increasingly more attention on the mechanism of core components of nanofat to promote wound healing. This paper reviewed the recent literatures on the mechanisms and clinical applications of nanofat in wound healing improvement.

Heart Defects, Congenital ; complications ; Hernia ; etiology ; Humans ; Infant ; Lung Diseases ; etiology ; Male ; Mediastinal Diseases ; etiology ; Pulmonary Atelectasis ; etiology

Objective To evaluate the effect of coagulation function changes on the incidence of acute kidney injury (AKI) after liver transplantation. Methods Clinical data of 245 liver transplant recipients who met the inclusion and exclusion criteria were retrospectively analyzed. According to the incidence of AKI after liver transplantation, all recipients were divided into the AKI group (n=99) and non-AKI group (n=146). The incidence of AKI after liver transplantation was summarized. Perioperative parameters of the recipients were collected. The risk factors of AKI after liver transplantation were assessed by univariate and multivariate analysis. Results Among 245 recipients undergoing liver transplantation, 99 cases developed AKI after operation with an incidence rate of 40.4%. Preoperative serum creatinine levels of the recipients and the in-hospital fatality were relatively high in the AKI group (all P < 0.05). Compared with the recipients in the non-AKI group, those in the AKI group presented with significantly higher liver function parameters within postoperative 24 h, significantly decreased levels of stage Ⅱ coagulation parameters including coagulation factorsⅤ, Ⅶ, Ⅸ, Ⅹ, Ⅻ and protein S, protein C and antithrombin Ⅲ, evidently elevated prothrombin time international normalized ratio (PT-INR), remarkably increased stage Ⅲ coagulation parameters including D-dimer and fibrin degradation product (FDP) levels and considerably decreased fibrinogen (FIB) level (all P < 0.05). Thrombelastogram showed that the R value was increased, the α angle was decreased and the coagulation time was prolonged in the AKI group (all P < 0.05). Logistic regression analysis demonstrated that the increased R value of postoperative thrombelastogram [odd ratio (OR) 1.116, 95% confidence interval (CI) 1.018-1.223, P=0.019], and decreased levels of antithrombin Ⅲ (OR 0.974, 95%CI 0.955-0.993, P=0.007) were the independent risk factors of incidence of AKI after liver transplantation. Conclusions The incidence of AKI after liver transplantation is high, which is associated with the coagulation function changes of the recipients. Decreased coagulation factor activity (increased R value) and declined antithrombin Ⅲ level are the independent risk factors of AKI in liver transplantat recipients.

Objective To discuss the health education needs of patients with hyperuricemia among Henlan district in Guangdong province so as to improve the effect of health education.Methods Adopted self-designed questionnaire survey forms,we conducted the investigation about content,time and way needs of the health education on 763 patients with hyperuricemia among coastal town in Guangdong from January to December 2011.Results Of the 733 distributed questionnaires,totally 689 questionnaire were retrieved,the effective resporvse rate was 94％.Influences of patient's age,time length of disease and time to select the health education on the patient' s demand of health education had the statistical significance (x2 =23.96,10.32,543.81; P ＜ 0.05).The demand of old patients regarding the health education reached 92.9％,and the demand factor of the patients with disease for 1-3 months about the health education was 93.4％,77.2％ of patients thought that the best time for receiving the health education was afternoon.And there was no significance difference in the education degree and the health education needs different of the patients (P ＞0.05).The choices of health education content needs of the patients were that 91％ of patients selected the prevention and treatment method and effect of the hyperuricemia,92.5％ of patients selected habits and customs which may easily cause the hyperuricemia,90％ of patients selected damage of the hyperuricemia.The choices of health education ways were as follows:91％ of patients selected the health knowledge lecture,90％ of patients selected the communication between doctor and patient,89％ of patients selected the introduction of ward mate' s experience.Conclusions Through the analysis on health education content and way needs of farmer group patients with hyperuricemia,more scientific,convenient and efficient health education services can be provided for patients.

Objective To analyze the effect of multi-disciplinary health education team on disease related knowledge and life style of patients with hyperuricemia.Methods Totals of 800 cases with hyperuricemia between January 2011 and June 2012 were randomly divided into observation group (n =400) and control group (n =400).Observation group received the intervention of multi-disciplinary health education team,while control group received the routine health education.Blood uric acid level were monitored and compared between two groups before and after health education.Through the questionnaire survey form,before and after health education,knowledge of two groups about prevention and control of hyperuricemia and life habits change situation were studied.Results Comparison of two groups of hyperuricemia curative effect,observation group was obviously higher than that of the control group [(91.25 ％ vs 53.00％) ;Z =-5.930,P ＜ 0.05)].No significant difference was found in the hyperuricemia knowledge awareness about prevention and control between two group before the intervention (P ＞ 0.05),while after the intervention,the knowledge awareness rote of health education group was significantly higher than that of the control group (94.50％ vs 66.25％ ;x2 =5.343,P ＜ 0.05).The reasonable diet proportion (96.75％ vs 53.25％),the proportion of drinking less than 200 ml (94.00％ vs 58.75 ％),the proportion of moderate exercise (91.25％ vs 66.75 ％) between two group were significant difference (x2 =5.231,5.038,4.987 ; P ＜ 0.05).Conclusions The intervention of multidisciplinary health education team on patients with hypemricemia can achieve the effect of prevention and control of hyperuricemia,and promote the change of patients' way of life,improve their awareness for hyperuricemia harmfulness,and improve their medical treatment compliance.

OBJECTIVETo explore the mucosal protective effect on the quality of gastric ulcer healing.

METHODSGastric ulcers were induced in male rats by serosal application of acetic acid. Rats were gavaged for 14 days with saline, omeprazole (OME), teprenone (TEP) and TEP plus OME starting 3 days after ulcer induction. Then the tissues and blood samples were obtained and measured.

RESULTThe lower ulcer index (UI) and increased ulcer inhibition rate were observed in OME and OME+TEP groups. In TEP and OME+TEP groups, restored mucosa thickness increased, cystically dilated glands decreased, microvessels in connective tissue increased, the secretion of mucus, hexosamine, PGE(2), bFGF were enhanced, the expression of EGFR was increased.

CONCLUSIONTEP can improve the quality of gastric ulcer healing, when combined with OME,the effect is more marked.

Acetic Acid ; Animals ; Anti-Ulcer Agents ; therapeutic use ; Diterpenes ; therapeutic use ; Drug Therapy, Combination ; Gastric Mucosa ; drug effects ; metabolism ; pathology ; Male ; Omeprazole ; therapeutic use ; Rats ; Rats, Wistar ; Receptor, Epidermal Growth Factor ; biosynthesis ; Secondary Prevention ; Stomach Ulcer ; chemically induced ; drug therapy ; pathology ; Wound Healing ; drug effects

Exosomes are 30-100 nm vesicles secreted from almost all types of cells.They contain various molecular constituents,including proteins,lipids,and RNA.As important mediators of cell-to-cell communication,exosomes are involved in a variety of physiological and pathological processes such as inflammatory reaction,cell proliferation and differentiation,tissue repair,immune signal transduction,and stress response.Exosomes can regulate and maintain the initiation and progression of many autoimmune diseases,especially rheumatoid arthritis.Meanwhile,exosomes may be a new biomarker for the diagnosis of rheumatoid arthritis and a potential treatment vector for this disease.
Arthritis, Rheumatoid ; pathology ; Cell Communication ; Exosomes ; Humans ; Signal Transduction

OBJECTIVETo analyze the expression of MAP2K4 and vimentin in human endometrial carcinoma (EC) and their association with the clinicopathological features and prognosis of the patients.

METHODSMAP2K4 and vimentin expressions were detected immunohistochemically in paraffin-embedded tissue sections from 128 patients with EC, and the correlation of MAP2K4 and vimentin expressions with the clinicopathological factors of the patients was analyzed.

RESULTSMAP2K4 and vimentin proteins were positively expressed in 49 (38.3%) and 83 (64.8%) of the patients, respectively. A positive expression of MAP2K4 was negatively correlated with FIGO stage of the tumor (P=0.010) and lymph node status (P=0.016); a positive expression of vimentin was positively correlated with FIGO stage of the tumor (P=0.025), histological grades (P=0.017), depth of myometrial invasion (P=0.044) and lymph node status (P=0.032). MAP2K4 was inversely associated with vimentin expression in EC(r=-0.598, P<0.001). Patients positive for MAP2K4 tended to have a higher overall survival rate (P=0.002), and those positive for vimentin tended to have a lower overall survival rate (P=0.007); patients positive for MAP2K4 but negative for vimentin had the longest survival time, while those negative for MAP2K4 and positive for vimentin had lowest survival rate (P=0.004).

CONCLUSIONDetection of MAP2K4 and vimentin might help in early diagnosis and prognostic evaluation of patients with EC.

Endometrial Neoplasms ; metabolism ; pathology ; Female ; Humans ; MAP Kinase Kinase 4 ; metabolism ; Prognosis ; Survival Rate ; Vimentin ; metabolism

OBJECTIVEThis study was designed to investigate the effect of human cytomegalovirus (HCMV) on the proliferation of colony forming unit granulocyte-macrophage (CFU-GM), CFU-erythroid (CFU-E), burst forming unit-erythroid (BFU-E), CFU-multipotential (CFU-Mix) and CFU-megakaryocytic (CFU-Mk) progenitor cells of cord blood in vitro as well as the possible mechanism.

METHODSTwenty cord blood specimens were collected from the umbilical vein of normal full-term neonates delivered spontaneously. This study consisted of five groups: 3 Infection groups in which 0.1 mL 10(3), 10(4) and 10(5) plague forming unit (PFU) HCMV-AD169 virus solution was added to the culture system, an Inactivated control group in which the equal volume of inactivated virus solution was added, and a Blank control group (normal progenitor cells culture system without HCMV virus infection). Colony forming unit-assay was applied to detect the effects of HCMV-AD169 strain on the colony formation, inhibition rate and colony-maintaining duration of CFU- GM, CFU-E, BFU-E, CFU-Mix and CFU-Mk of cord blood. PCR technique was used to demonstrate the existence of HCMV-DNA in the colony cells of cultured CFU-GM, CFU-E, CFU-Mix and CFU-Mk.

RESULTSHCMV-AD169 (10(3)PFU) in low concentration had inhibition effects on colony formation of the CFU-Mix and CFU-Mk (P < 0.05), whereas 10(5) PFU and 10(4) PFU HCMV-AD169 lead to decreased colonies in CFU-GM, CFU-E, BFU-E, CFU-Mix and CFU-Mk compared with the Blank control and the Inactivated control groups (P < 0.05). The suppression effect of HCMV on the colony formation was dose-dependent. The colony-maintaining duration of the CFU-GM, CFU-E, BFU-E, CFU-Mix and CFU-Mk in the 10(5) PFU and 10(4) PFU HCMV infection groups was significantly shorter than that in the two control groups (P < 0.01). The low concentration of HCMV-AD169 (10(3)PFU) infection resulted in a shortened colony-maintaining duration of the CFU-Mix and CFU-Mk (P < 0.01), but had no effects on the colony-maintaining duration of CFU-GM, CFU-E and BFU-E. PCR amplification demonstrated the existence of HCMV-AD169 DNA in the colony cells of the three Infection groups.

CONCLUSIONSHCMV-AD169 strain can infect hematopoietic progenitors of cord blood and inhibit the proliferation of hematopoietic progenitors, associated with anemia, neutropenia and thrombocytopenia in HCMV patients.

Cell Proliferation ; Cytomegalovirus ; pathogenicity ; Fetal Blood ; cytology ; Hematopoietic Stem Cells ; cytology ; Humans