Nanofat is a chylous fluid obtained by mechanical emulsification and filtration of adipose tissue, which can pass through 27 G needle and be used for wound treatment, scar repair, skin rejuvenation, etc. In recent years, some researchers have proposed modified procedures based on the traditional preparation procedures of nanofat. Meanwhile, there has been increasingly more attention on the mechanism of core components of nanofat to promote wound healing. This paper reviewed the recent literatures on the mechanisms and clinical applications of nanofat in wound healing improvement.

Nanofat is a chylous fluid obtained by mechanical emulsification and filtration of adipose tissue, which can pass through 27 G needle and be used for wound treatment, scar repair, skin rejuvenation, etc. In recent years, some researchers have proposed modified procedures based on the traditional preparation procedures of nanofat. Meanwhile, there has been increasingly more attention on the mechanism of core components of nanofat to promote wound healing. This paper reviewed the recent literatures on the mechanisms and clinical applications of nanofat in wound healing improvement.

Heart Defects, Congenital ; complications ; Hernia ; etiology ; Humans ; Infant ; Lung Diseases ; etiology ; Male ; Mediastinal Diseases ; etiology ; Pulmonary Atelectasis ; etiology

Objective To evaluate the effect of coagulation function changes on the incidence of acute kidney injury (AKI) after liver transplantation. Methods Clinical data of 245 liver transplant recipients who met the inclusion and exclusion criteria were retrospectively analyzed. According to the incidence of AKI after liver transplantation, all recipients were divided into the AKI group (n=99) and non-AKI group (n=146). The incidence of AKI after liver transplantation was summarized. Perioperative parameters of the recipients were collected. The risk factors of AKI after liver transplantation were assessed by univariate and multivariate analysis. Results Among 245 recipients undergoing liver transplantation, 99 cases developed AKI after operation with an incidence rate of 40.4%. Preoperative serum creatinine levels of the recipients and the in-hospital fatality were relatively high in the AKI group (all P < 0.05). Compared with the recipients in the non-AKI group, those in the AKI group presented with significantly higher liver function parameters within postoperative 24 h, significantly decreased levels of stage Ⅱ coagulation parameters including coagulation factorsⅤ, Ⅶ, Ⅸ, Ⅹ, Ⅻ and protein S, protein C and antithrombin Ⅲ, evidently elevated prothrombin time international normalized ratio (PT-INR), remarkably increased stage Ⅲ coagulation parameters including D-dimer and fibrin degradation product (FDP) levels and considerably decreased fibrinogen (FIB) level (all P < 0.05). Thrombelastogram showed that the R value was increased, the α angle was decreased and the coagulation time was prolonged in the AKI group (all P < 0.05). Logistic regression analysis demonstrated that the increased R value of postoperative thrombelastogram [odd ratio (OR) 1.116, 95% confidence interval (CI) 1.018-1.223, P=0.019], and decreased levels of antithrombin Ⅲ (OR 0.974, 95%CI 0.955-0.993, P=0.007) were the independent risk factors of incidence of AKI after liver transplantation. Conclusions The incidence of AKI after liver transplantation is high, which is associated with the coagulation function changes of the recipients. Decreased coagulation factor activity (increased R value) and declined antithrombin Ⅲ level are the independent risk factors of AKI in liver transplantat recipients.

Objective To discuss the health education needs of patients with hyperuricemia among Henlan district in Guangdong province so as to improve the effect of health education.Methods Adopted self-designed questionnaire survey forms,we conducted the investigation about content,time and way needs of the health education on 763 patients with hyperuricemia among coastal town in Guangdong from January to December 2011.Results Of the 733 distributed questionnaires,totally 689 questionnaire were retrieved,the effective resporvse rate was 94％.Influences of patient's age,time length of disease and time to select the health education on the patient' s demand of health education had the statistical significance (x2 =23.96,10.32,543.81; P ＜ 0.05).The demand of old patients regarding the health education reached 92.9％,and the demand factor of the patients with disease for 1-3 months about the health education was 93.4％,77.2％ of patients thought that the best time for receiving the health education was afternoon.And there was no significance difference in the education degree and the health education needs different of the patients (P ＞0.05).The choices of health education content needs of the patients were that 91％ of patients selected the prevention and treatment method and effect of the hyperuricemia,92.5％ of patients selected habits and customs which may easily cause the hyperuricemia,90％ of patients selected damage of the hyperuricemia.The choices of health education ways were as follows:91％ of patients selected the health knowledge lecture,90％ of patients selected the communication between doctor and patient,89％ of patients selected the introduction of ward mate' s experience.Conclusions Through the analysis on health education content and way needs of farmer group patients with hyperuricemia,more scientific,convenient and efficient health education services can be provided for patients.

Objective To analyze the effect of multi-disciplinary health education team on disease related knowledge and life style of patients with hyperuricemia.Methods Totals of 800 cases with hyperuricemia between January 2011 and June 2012 were randomly divided into observation group (n =400) and control group (n =400).Observation group received the intervention of multi-disciplinary health education team,while control group received the routine health education.Blood uric acid level were monitored and compared between two groups before and after health education.Through the questionnaire survey form,before and after health education,knowledge of two groups about prevention and control of hyperuricemia and life habits change situation were studied.Results Comparison of two groups of hyperuricemia curative effect,observation group was obviously higher than that of the control group [(91.25 ％ vs 53.00％) ;Z =-5.930,P ＜ 0.05)].No significant difference was found in the hyperuricemia knowledge awareness about prevention and control between two group before the intervention (P ＞ 0.05),while after the intervention,the knowledge awareness rote of health education group was significantly higher than that of the control group (94.50％ vs 66.25％ ;x2 =5.343,P ＜ 0.05).The reasonable diet proportion (96.75％ vs 53.25％),the proportion of drinking less than 200 ml (94.00％ vs 58.75 ％),the proportion of moderate exercise (91.25％ vs 66.75 ％) between two group were significant difference (x2 =5.231,5.038,4.987 ; P ＜ 0.05).Conclusions The intervention of multidisciplinary health education team on patients with hypemricemia can achieve the effect of prevention and control of hyperuricemia,and promote the change of patients' way of life,improve their awareness for hyperuricemia harmfulness,and improve their medical treatment compliance.

OBJECTIVETo explore the mucosal protective effect on the quality of gastric ulcer healing.

METHODSGastric ulcers were induced in male rats by serosal application of acetic acid. Rats were gavaged for 14 days with saline, omeprazole (OME), teprenone (TEP) and TEP plus OME starting 3 days after ulcer induction. Then the tissues and blood samples were obtained and measured.

RESULTThe lower ulcer index (UI) and increased ulcer inhibition rate were observed in OME and OME+TEP groups. In TEP and OME+TEP groups, restored mucosa thickness increased, cystically dilated glands decreased, microvessels in connective tissue increased, the secretion of mucus, hexosamine, PGE(2), bFGF were enhanced, the expression of EGFR was increased.

CONCLUSIONTEP can improve the quality of gastric ulcer healing, when combined with OME,the effect is more marked.

Acetic Acid ; Animals ; Anti-Ulcer Agents ; therapeutic use ; Diterpenes ; therapeutic use ; Drug Therapy, Combination ; Gastric Mucosa ; drug effects ; metabolism ; pathology ; Male ; Omeprazole ; therapeutic use ; Rats ; Rats, Wistar ; Receptor, Epidermal Growth Factor ; biosynthesis ; Secondary Prevention ; Stomach Ulcer ; chemically induced ; drug therapy ; pathology ; Wound Healing ; drug effects

Exosomes are 30-100 nm vesicles secreted from almost all types of cells.They contain various molecular constituents,including proteins,lipids,and RNA.As important mediators of cell-to-cell communication,exosomes are involved in a variety of physiological and pathological processes such as inflammatory reaction,cell proliferation and differentiation,tissue repair,immune signal transduction,and stress response.Exosomes can regulate and maintain the initiation and progression of many autoimmune diseases,especially rheumatoid arthritis.Meanwhile,exosomes may be a new biomarker for the diagnosis of rheumatoid arthritis and a potential treatment vector for this disease.
Arthritis, Rheumatoid ; pathology ; Cell Communication ; Exosomes ; Humans ; Signal Transduction

AIM:To observe the effect of thyroxine on the expression of T-type calcium channels Cav3. 1, Cav3. 2 and Cav3. 3 in rat myocardium, and to explore the possible biological mechanism between the changes of the ex-pression of T-type calcium channels and the arrhythmia in hyperthyroid heart disease. METHODS:Healthy SD rats (n=20) were randomly divided into normal control group (n=10) and hyperthyroid heart disease group (n=10). The animal model was established by intraperitoneal injection of levothyroxine for 35 d. The contents of T3 and T4 in serum, the heart-to-body weight ratio, the diameter of cardiac myocytes and electrocardiograph were measured to evaluate hyperthyroid heart disease. Moreover, the mRNA and protein expression levels of T-type calcium channels in the myocardium were measured by RT-PCR, immunohistochemistry and Western blot. RESULTS:After intraperitoneal injection of levothyroxine for 35 d, compared with the normal control group, the serum contents of T3 and T4, the heart-to-body weight ratio and the diameter of cardiac myocytes were significantly increased in hyperthyroid heart disease group (P<0.05), and arrhythmia occurred in hyperthyroid heart disease group. By immunohistochemistry and Western blot, the protein expression of Cav3. 1 in-creased significantly (P<0.05), while the protein expression of Cav3.2 decreased significantly (P<0.01). However, no change of the Cav3. 3 protein was observed. The results of RT-PCR were the same as immunohistochemistry and Western blot. CONCLUSION:Thyroxine promotes the expression of Cav3. 1 in the myocardium but inhibits the expression of Cav3. 2 at mRNA and protein levels, which might be involved in arrhythmia in hyperthyroid heart disease.

OBJECTIVETo investigate the application of titanium mesh and free forearm flap for reconstruction of maxillary defect resulted from tumor resection.

METHODSFrom 2004 to 2008, 19 cases with maxillary tumor underwent tumor resection. The defects were reconstructed immediately with titanium mesh for bony defects and free forearm flap for oral mucosa defects.

RESULTS16 cases achieved satisfactory functional and cosmetic results. The speech assessment was good without oronasal reflux. The titanium mesh was exposed and infected in 3 cases. Then the meshes were taken out and the defects were covered with the forearm flap to close the oronasal fistula. The midface was slightly depressed with no functional morbidity.

CONCLUSIONSIt is simple and practical to reconstruct maxillary defect with titanium mesh and free forearm flap. Both the functional and cosmetic results are satisfactory.

Adolescent ; Adult ; Aged ; Female ; Forearm ; surgery ; Humans ; Male ; Maxillary Neoplasms ; surgery ; Middle Aged ; Reconstructive Surgical Procedures ; methods ; Skin Transplantation ; Surgical Flaps ; Surgical Mesh ; Titanium ; Young Adult