Nanofat is a chylous fluid obtained by mechanical emulsification and filtration of adipose tissue, which can pass through 27 G needle and be used for wound treatment, scar repair, skin rejuvenation, etc. In recent years, some researchers have proposed modified procedures based on the traditional preparation procedures of nanofat. Meanwhile, there has been increasingly more attention on the mechanism of core components of nanofat to promote wound healing. This paper reviewed the recent literatures on the mechanisms and clinical applications of nanofat in wound healing improvement.

Nanofat is a chylous fluid obtained by mechanical emulsification and filtration of adipose tissue, which can pass through 27 G needle and be used for wound treatment, scar repair, skin rejuvenation, etc. In recent years, some researchers have proposed modified procedures based on the traditional preparation procedures of nanofat. Meanwhile, there has been increasingly more attention on the mechanism of core components of nanofat to promote wound healing. This paper reviewed the recent literatures on the mechanisms and clinical applications of nanofat in wound healing improvement.

Heart Defects, Congenital ; complications ; Hernia ; etiology ; Humans ; Infant ; Lung Diseases ; etiology ; Male ; Mediastinal Diseases ; etiology ; Pulmonary Atelectasis ; etiology

Objective To evaluate the effect of coagulation function changes on the incidence of acute kidney injury (AKI) after liver transplantation. Methods Clinical data of 245 liver transplant recipients who met the inclusion and exclusion criteria were retrospectively analyzed. According to the incidence of AKI after liver transplantation, all recipients were divided into the AKI group (n=99) and non-AKI group (n=146). The incidence of AKI after liver transplantation was summarized. Perioperative parameters of the recipients were collected. The risk factors of AKI after liver transplantation were assessed by univariate and multivariate analysis. Results Among 245 recipients undergoing liver transplantation, 99 cases developed AKI after operation with an incidence rate of 40.4%. Preoperative serum creatinine levels of the recipients and the in-hospital fatality were relatively high in the AKI group (all P < 0.05). Compared with the recipients in the non-AKI group, those in the AKI group presented with significantly higher liver function parameters within postoperative 24 h, significantly decreased levels of stage Ⅱ coagulation parameters including coagulation factorsⅤ, Ⅶ, Ⅸ, Ⅹ, Ⅻ and protein S, protein C and antithrombin Ⅲ, evidently elevated prothrombin time international normalized ratio (PT-INR), remarkably increased stage Ⅲ coagulation parameters including D-dimer and fibrin degradation product (FDP) levels and considerably decreased fibrinogen (FIB) level (all P < 0.05). Thrombelastogram showed that the R value was increased, the α angle was decreased and the coagulation time was prolonged in the AKI group (all P < 0.05). Logistic regression analysis demonstrated that the increased R value of postoperative thrombelastogram [odd ratio (OR) 1.116, 95% confidence interval (CI) 1.018-1.223, P=0.019], and decreased levels of antithrombin Ⅲ (OR 0.974, 95%CI 0.955-0.993, P=0.007) were the independent risk factors of incidence of AKI after liver transplantation. Conclusions The incidence of AKI after liver transplantation is high, which is associated with the coagulation function changes of the recipients. Decreased coagulation factor activity (increased R value) and declined antithrombin Ⅲ level are the independent risk factors of AKI in liver transplantat recipients.

Objective To discuss the health education needs of patients with hyperuricemia among Henlan district in Guangdong province so as to improve the effect of health education.Methods Adopted self-designed questionnaire survey forms,we conducted the investigation about content,time and way needs of the health education on 763 patients with hyperuricemia among coastal town in Guangdong from January to December 2011.Results Of the 733 distributed questionnaires,totally 689 questionnaire were retrieved,the effective resporvse rate was 94％.Influences of patient's age,time length of disease and time to select the health education on the patient' s demand of health education had the statistical significance (x2 =23.96,10.32,543.81; P ＜ 0.05).The demand of old patients regarding the health education reached 92.9％,and the demand factor of the patients with disease for 1-3 months about the health education was 93.4％,77.2％ of patients thought that the best time for receiving the health education was afternoon.And there was no significance difference in the education degree and the health education needs different of the patients (P ＞0.05).The choices of health education content needs of the patients were that 91％ of patients selected the prevention and treatment method and effect of the hyperuricemia,92.5％ of patients selected habits and customs which may easily cause the hyperuricemia,90％ of patients selected damage of the hyperuricemia.The choices of health education ways were as follows:91％ of patients selected the health knowledge lecture,90％ of patients selected the communication between doctor and patient,89％ of patients selected the introduction of ward mate' s experience.Conclusions Through the analysis on health education content and way needs of farmer group patients with hyperuricemia,more scientific,convenient and efficient health education services can be provided for patients.

Objective To analyze the effect of multi-disciplinary health education team on disease related knowledge and life style of patients with hyperuricemia.Methods Totals of 800 cases with hyperuricemia between January 2011 and June 2012 were randomly divided into observation group (n =400) and control group (n =400).Observation group received the intervention of multi-disciplinary health education team,while control group received the routine health education.Blood uric acid level were monitored and compared between two groups before and after health education.Through the questionnaire survey form,before and after health education,knowledge of two groups about prevention and control of hyperuricemia and life habits change situation were studied.Results Comparison of two groups of hyperuricemia curative effect,observation group was obviously higher than that of the control group [(91.25 ％ vs 53.00％) ;Z =-5.930,P ＜ 0.05)].No significant difference was found in the hyperuricemia knowledge awareness about prevention and control between two group before the intervention (P ＞ 0.05),while after the intervention,the knowledge awareness rote of health education group was significantly higher than that of the control group (94.50％ vs 66.25％ ;x2 =5.343,P ＜ 0.05).The reasonable diet proportion (96.75％ vs 53.25％),the proportion of drinking less than 200 ml (94.00％ vs 58.75 ％),the proportion of moderate exercise (91.25％ vs 66.75 ％) between two group were significant difference (x2 =5.231,5.038,4.987 ; P ＜ 0.05).Conclusions The intervention of multidisciplinary health education team on patients with hypemricemia can achieve the effect of prevention and control of hyperuricemia,and promote the change of patients' way of life,improve their awareness for hyperuricemia harmfulness,and improve their medical treatment compliance.

OBJECTIVETo explore the mucosal protective effect on the quality of gastric ulcer healing.

METHODSGastric ulcers were induced in male rats by serosal application of acetic acid. Rats were gavaged for 14 days with saline, omeprazole (OME), teprenone (TEP) and TEP plus OME starting 3 days after ulcer induction. Then the tissues and blood samples were obtained and measured.

RESULTThe lower ulcer index (UI) and increased ulcer inhibition rate were observed in OME and OME+TEP groups. In TEP and OME+TEP groups, restored mucosa thickness increased, cystically dilated glands decreased, microvessels in connective tissue increased, the secretion of mucus, hexosamine, PGE(2), bFGF were enhanced, the expression of EGFR was increased.

CONCLUSIONTEP can improve the quality of gastric ulcer healing, when combined with OME,the effect is more marked.

Acetic Acid ; Animals ; Anti-Ulcer Agents ; therapeutic use ; Diterpenes ; therapeutic use ; Drug Therapy, Combination ; Gastric Mucosa ; drug effects ; metabolism ; pathology ; Male ; Omeprazole ; therapeutic use ; Rats ; Rats, Wistar ; Receptor, Epidermal Growth Factor ; biosynthesis ; Secondary Prevention ; Stomach Ulcer ; chemically induced ; drug therapy ; pathology ; Wound Healing ; drug effects

Exosomes are 30-100 nm vesicles secreted from almost all types of cells.They contain various molecular constituents,including proteins,lipids,and RNA.As important mediators of cell-to-cell communication,exosomes are involved in a variety of physiological and pathological processes such as inflammatory reaction,cell proliferation and differentiation,tissue repair,immune signal transduction,and stress response.Exosomes can regulate and maintain the initiation and progression of many autoimmune diseases,especially rheumatoid arthritis.Meanwhile,exosomes may be a new biomarker for the diagnosis of rheumatoid arthritis and a potential treatment vector for this disease.
Arthritis, Rheumatoid ; pathology ; Cell Communication ; Exosomes ; Humans ; Signal Transduction

AIMTo study the metabolic pathways of ginsenoside Rd in rats.

METHODSUrine samples were collected before and after 24 h of single oral administration of 150 mg and intravenous administration of 60 mg of ginsenoside Rd to six rats, separately. The samples were purified by SPE column and then were analyzed by liquid chromatography-ESI-mass spectrometry for putative metabolites.

RESULTSParent drug and its seven metabolites were identified in rat urine based on comparing total ion chromatograms of the blank with the metalolic urine as well as mass spectra. Its main metabolic pathways and possible structures are elucidated.

CONCLUSIONOxidation, combination and deglucosylation were found to be the major metabolic pathway of ginsenoside Rd in rats.

Administration, Oral ; Animals ; Chromatography, High Pressure Liquid ; methods ; Ginsenosides ; administration & dosage ; metabolism ; urine ; Injections, Intravenous ; Male ; Oxidation-Reduction ; Panax ; chemistry ; Plants, Medicinal ; chemistry ; Rats ; Rats, Sprague-Dawley ; Spectrometry, Mass, Electrospray Ionization ; methods

OBJECTIVETo investigate the role of specificity protein 1 (Sp1) in regulating radiosensitivity of cervical cancer cell lines.

METHODSWe analyzed Sp1 expression in 6 different cervical cancer cell lines (SiHa, HeLa, Caski, Me180, Ms751, and C33a) using Western blotting and real-time PCR. Clonogenic survival assay and curve fitting were used to assess the changes in radiosensitivity of Me180 cells transfected with lentivirus-mediated shRNA vector targeting sp1 and HeLa cells transfected with sp1 over-expression vector.

RESULTSIn the 6 cell lines tested, the cellular expression levels of Sp1 decreased gradually in the order of Me180, Caski, C33a, SiHa, Ms751, and HeLa. SP1 knockdown with lentivirus-mediated shRNA significantly lowered the survival rate of Me180 cells following radiation exposure (P<0.05), and obviously lowered the values of SF2, D0 and Dq but significantly increased α/β of the cells. Compared with the cells transfected with the mock vector, HeLa cells with sp1 over-expression showed a significantly increased survival following radiation exposure (P<0.05) with obviously increased values of SF2, D0 and Dq but significantly lowered α/β.

CONCLUSIONSilencing Sp1 can increase the radiosensitivity while Sp1 overexpression enhances the radioresistance of cervical cancer cell lines, suggesting an important role of Sp1 in radiotherapy for cervical cancer.