Thrombospondin(TSP) has five members with diversiform expressions and complicated biologic functions in different histiocytes all over the body.TSP also is widely expressed in different parts of eye,such as retinal pigment epithelial cells,corneal endothelial cells and lens epithelial cells,etc.,and sometimes TSP is secreted by trabecular meshwork cells.As a group of cytokines,TSP plays an important regulating role in the physiological and pathological activities of eye.Studies determined that TSP participates in the pathogenesis of age-related macular degeneration,repaired of corneal diseases,formation of cataract,development of open-angle glaucoma,pathological process of eye tumor,occurrence of proliferating vitroretinopathy and diabetic retinopathy.Studies towards their functions are helpful for revealing the pathogenesis of eye diseases and providing new ideas of prevention and therapy.The distribution,source and eye disease-related action of TSP were summarized.

There is a high incidence of Lung cancer among the miners of Yunnan Tin Mine.In or- der to study the carcinogenicity of chemical elements with none radioactive in the mine pow- der,a mixture of seven non-radioactive,inorganic chemical elements(CM_2:As,Pb,Fe, Cr,Ni,Cd and Be)relating to mutagenicity and tumorgenesis were chosen for Ames test and carinoma inducing experiments in hamsters.The results are as follows:1 Ames test of CM_2is positive.2 The lung cancer inducing experiment of CM_2on hamsters is successful.Lung cancer and precancerous lesions develop in 6 of 27 effective animals, the incidence is 22.2%.No tu- mor was found in the control group.The results show that CM_2is related to the lung cancer formation of hamsters,tihs is reported for the first time.

There is a high incidence of malignant lung tumor in Yunnan tin mine area.In order to detect the causes,the mixtures of artificial synthetic mine dust containing As,Pb,Fe etc(No. 1,2,3)were used for tumor-inducing experiments on Wistar rats.The results were as follows: 31 lung sarcomas were developed in 3 experimental groups and the incidence was 22.6%.The malignant lung lymphomas developed in 28 of 31 sarcomas,the rate being 90.2%.In addition, 3 cases of lung cancer were found.No benign or malignant lung tumor was found in the con- trol group.The results showed that the artificial synthetic mine dust(No.1,2,3)was related to the lung sarcoma formation on Wistar rats,which is reported for the first time.

There is a high incidence of lung cancer among the miners of Yunnan Tin Mine. We have successfully induced lung cancer in hamsters separately by mine powder, mine powder plus radon or flue dust of the refinery. There are more than thirty similar chemical elements in both mine and flue dust. In order to exclude the action of the trace radioactive chemical elements and to confirm the carcinogenic effect of the non-radioactive inorganic chemical substances, We choose vanadium (V) and zirconium (Zr) to carry out cell transformation test in vitro. The results are as follows: 1. The cell toxicity test of V and Zr is positive.2. The cell transformation test of V shows positive and that of Zr is negative. This is reported for the first time.

95%),the dynamic expressions of TGF-?1,MMP-2 and MMP-9mRNA in lung for each hyperoxia group were measured by reverse transcript polymerse chain reaction(RT-PCR).The protein content in bronchoalveolar lavage fluid(BALF),permeability index,lung wet/dry weight ratio and pathological changes of lung tissues were all tested.Results:The level of TGF-?1mRNA in hyperoxia group increased from the 3rd day,and rose gradually until the 14th day.The expressions of MMP-2 and MMP-9 mRNA increased markedly from the 3rd day,rose to the peak at the 7th day,and decreased at the 14th day.The above changes accompanied with lung pathological manifestations,which included acute alveolitis during the earlier period and lung fibrosis during later period.Conclusions:The unbalance between TGF-?1 and MMPs may play an important role in the development of lung fibrosis caused by hyperoxia-induced lung injury.

OBJECTIVE: To prepare ofloxacin liposomes for increasing bacteria sensitivity, and lo evaluate its in vitro properly. METHODS: Ofloxacin liposomes were prepared by pH gradient method using phospholipid and cholesterol as materials. The effects of pH value and the warming temperature and time on encapsulation efficiency were investigated. The particle size of liposomes was determined and the morphology was investigated by transmission electric microscope (TEM). The in vitro release experiment was carried out vising physiological saline as the medium. The bacteriostatic action against Staphylococcus aureus was investigated using disc diffusion test and dilution test. RESULTS: The maximum encapsulation efficiency of 82.40% could be achieved when pH value was 7.0, warming temperature was 50°C and warming time was 5 min. The average particle size of the liposomes was 174nm, and the ofloxacin liposomes showed good morphology under TEM. The liposomes could release ofloxacin in a sustained manner in vitro. The ofloxacin liposomes and solution showed same inhibition zones in disc diffusion test, while their minimal inhibitory concentrations determined by dilution test were 0.39 and 0.78μg · mL-1, respectively. CONCLUSION: Ofloxacin liposomes can be prepared by pH gradient method with high encapsulation efficiency, and the bacteriostatic effect of ofloxacin against Staphylococcus aureus can be improved by incorporating it into liposomes.

Objective: To explore the effects and mechanisms of polydatin on rats with brain hemor-rhagic injury. Methods: The cerebral hemorrhage model in rats was induced by local injection of type VII collagenase and they were randomly divided into six groups. The treated groups were treated with polydatin and Naoxuekang; The normal and control groups were only treated with water. The changes of neuro logical defect were observed. The activity of SOD, the content of MDA, the protein expressions of Bcl-2 in brain tissue, and the contents of IL-1β in serum were measured. Results: Compared with the cerebral hemorrhage model group, polydatin (25, 50, and 100 mg/kg) could significantly reduce the symptoms of neurological deficits of cerebral hemorrhage rats, raise the activity of SOD, reduce the content of MDA, increase the protein expressions of Bcl-2, and decrease the contents of IL-1β in serum. Its effects were pos-itively correlated with the dose-effect. Conclusion: Polydatin has therapeutic effect on rats of cerebral hemorrhage, through against free radical damage and inhibiting neuronal apoptosis and inflammatory.

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Angioplasty, Balloon, Coronary ; methods ; Coronary Disease ; therapy ; Coronary Thrombosis ; etiology ; Drug Delivery Systems ; Humans ; Paclitaxel ; administration & dosage ; Sirolimus ; administration & dosage ; Stents ; adverse effects

BACKGROUND:Piperine in models of pancreatitis, gout, middle cerebral artery infarction has anti-inflammatory, antioxidant and immune regulatory effects, but its effects on periodontitis model are not clear.
OBJECTIVE:To observe the protective effect of piperine on bone absorption and degradation of col agen in experimental rat models of periodontitis.
METHODS:Rat models of periodontitis were established by ligaturing the dental cervix of rat mandibular first molar with 3-0 silk. On day 1 before model establishment, rats were intragastrical y administered piperine 50 and 100 mg/kg. There were healthy control group and model group. Related detection was performed 8 weeks after model establishment.
RESULTS AND CONCLUSION:(1) Results of quantitative CT analysis:compared with the healthy control group, the distance from the first molar enamelo-cemental junction to the alveolar ridge crest was significantly lower in the model group (P<0.05). The degree of alveolar damage was significantly improved in the 50 and 100 mg/kg piperine groups compared with the model group (P<0.05). (2) Related factor protein expression:compared with the model group, matrix metal oproteinase-8,-13 and interleukin-1βprotein expression was significantly decreased in the 100 mg/kg piperine group (P<0.05);matrix metal oproteinase-8 protein expression was significantly decreased in the 50 mg/kg piperine group (P<0.05). (3) Col agen fiber morphology:compared with the model group, col agen fibers arranged orderly and col agen fiber area significantly increased in the 50 and 100 mg/kg piperine groups (P<0.05). (4) Results confirmed that piperine could reduce the alveolar bone resorption, reduce the degradation of col agen fibers and protect the periodontal tissues in models of periodontitis. Its mechanism is associated with the inhibition of matrix metal oproteinase-8,-13 and interleukin-1βprotein expression.