Metronidazole derivative (CM) administered intraperitoneally or intravenously could cause a marked dose dependent neurotoxic response.The mean LD50 was 5034 mg/kg in the three batches of CM intraperitoneally administered and their effect was statistically identical, and the mean LD50 was 3186 mg/kg in the three baches of CM intravenously administered and their effect was same although there was a significant difference between 8638 and 8646 batches which was probably due to the individual difference in Sensitivity to intravenous injection.

In this experiment, we studied the effects of rhIL-3, rhlFN-r and DMSO on the growth and differentiation of the human leukemia cell line HL-60. The results showed that three agents were capable of suppressing the proliferation of the HL-60 cells and inducing terminal differentiation of the cells, with the exception of early augmentation of the cell growth by the rhIL-3. The differentiation effect was most obvious in DMSO treated cells, then in rhIL-3 and least in rhIFN-r The enhanced effects occurred when the HL-60 cells were treated in combination with DMSO and rhIL-3 or rhIFN-r

In this study, several physiological factors were studied for their effects on the growth and differentiation of U937 cells in vitro .The results showed that (1)rhTNF-?,PHA-LCM and c-RA exerted a dose and time-dependent suppression of proliferation of U937 cells; (2) the synergistic effect oc-cured when rhTNF-? was combined with c-RA in the cell culture; and (3)rhIL-1-? augmented the differentiation of U937 cells induced by c-RA though it had no effect on the cell line by itself. According to the [3H]-TdR incorporation, it can be concluded that the reduction of DNA synthesis of U917 cells by rhTNF-? ,PHA-LCM,and c-RA is attributed to the inhibition of the growth. The results might have clinical significance in the induction of differentiation of leukemia cells.

Objective To study the correlation between expression of vascular endothelial growth factor(VEGF)and tissue inhibitor of metalloproteinase 2(TIMP-2)and prognosis of patients with laryngeal squamous cell carcinoma.Methods The expression of VEGF and TIMP-2 were measured in 38 specimen of LSCC and 19 specimens of normal laryngeal tissue adjacent to the tumors with EnVision immunohistochemical technique,follow-up for 3~5 years after operation local relapse occurred in four cases,cervical nodular metastasized occurred in fwo cases and pneumonic metastasis occurred one case.Results The expression level of two marks in primary tumors was significantly higher than that in pericancerous tissues(P

Objective:To analyze and evaluate the usage and tendency of quinolones before and after the special rectification of antibacterial in one grade III level A hospital in üanjing to provide evidence for the special rectification of antibacterial and manage-ment. Methods:The usage records of quinolones in 2010( before the special rectification)and in 2011-2013( after the special rectifi-cation)were collected,and the sales revenue,DDDs,defined daily cost( DDC),drug resistance and adverse drug reactions( ADR) were analyzed retrospectively. Results:Both the amount and ratio of the sales revenue of quinolones were declined dramatically after the rectification(2011-2013). Among all kinds of quinolones,the sales revenue and DDDs of lomefloxacin aspartate injections was the highest before the rectification while moxifloxacin injections and levofloxacine hydrochloride injections ranked the first and the second respectively in sales revenue after the rectification(2012-2013). As for the DDDs,levofloxacin hydrochloride tablets and levofloxacine hydrochloride injections were the highest after the rectification. Besides,moxifloxacin injections had the highest DDC after the rectifica-tion. Moreover,the drug resistance of the main drug-resistant bacteria( namely staphylococcus,enterobacteriaceae and non fermenta-tion bacteria)to levofloxacin,norfloxacin and ciprofloxacin was declined after the rectification. The adverse drug reactions of levofloxa-cin,lomefloxacin,enoxacin and moxifloxacin were declined year by year after the rectification,and the ADR ratio of quinolones in all anti-bacterial agents was also declined. Conclusion:After the special rectification of antibacterials,the hospital exhibits obvious im-provement in all the application indices of antibacterials,reflecting a good result of the special rectification. However,the hospital still needs to strengthen the inspection over the clinical application of the fourth generation of quinolones.

Objective To evaluate the clinical effect of postural reduction and bracing on thoracolumbar fractures without neurological deficit.Methods21 patients with thoracolumbar fractures(T11～L2

【Objective】 Selenium (Se) contents of some chemical forms as total Se,inorganic Se,organic Se and selenomethionine (SeMet) in rice e nriched-Se were determined, in order to provide scientific basis for applying r ice enriched-Se to cancer prevention. 【Methods】 Fluorometric determination wi th 2,3-diaminonaphthalene (2,3-DAN) for total Se, inorganic Se and organic Se, and with CNBr-2,3-DAN for SeMet. 【Results】 Total Se contents of rice enrich ed-Se reaping in summer and autumn were (2.71±0.97) μg/g, (9.87±1.18 ) μg/g, respectively, both higher than that of ordinary rice (P<0.001); the organic Se occupying over 99% of total Se were 2.68, 9.77 μg/g, respectively . Main component of the organic Se was SeMet, and its Se content (Se-SeMet) was (1.45±0.67) μg/g for rice enriched-Se reaping in summer, (4.96±0.98) μ g/g in autumn,and their contents/total Se contents were 53.5, 50.5 %, respecti vely. 【Conclusions】 Most part of Se in rice enriched-Se was natural organic Se and main component of the organic Se was SeMet.

Objective: To better understand homing potentiality of hematopoietic stem cells (HSC) in human umbilial cord blood (UCB) and the mechanism of dextran sulfate (DS) mobilization. Methods: Sub lethally irradiated or DS pretreated severe combined immunodeficient (SCID) mice were transplanted with UCB, which was cryopreserved at -80℃.Human cells in recipient mice were detected by flow cytometry and CFU GM assay from each host organ. Results: In contrast with the controls, engraftment after irradiation or administration of DS resulted in a higher percentage of CD45 +,CD34 +,CD19 + cells produced in SCID mice. While comparison between the experimental groups, higher implantation level was obtained in the former if equivalent donor cells were used in both groups. Conclusion:DS is a safe and effective pretreatment, which can mobilize HSC, but also vocate niches for transplanted HSC homing. [

Objective To evaluate brush cytology under endoscopic retrograde cholangiopancreatography (ERCP) for malignant biliary strictures, and to analyze the factors influencing the diagnosis yield.Methods Brush cytology was applied in 144 patients with suspected malignant biliary strictures at ERCP.Brushing in bile duct was performed for 10 times from 2004 to 2006, while at least 20 times of brushing and repeated manipulation were performed at biliary strictures from 2007 to 2009. Cytological samples were processed immediately after brushing and analyzed by the same pathologist. Efficacy of brush diagnosis was evaluated based on reference to histopathology after surgery and/or clinical diagnostic criteria. Results Malignant stricture was finally diagnosed in 96 patients, and benign diseases in other 48. Brush cytology was positive in 78 of 96 patients with malignant stricture ( sensitivity 81.3% ), and negative in all patients without malignancy ( specificity 100. 0% ). Overall accuracy of diagnosis was 87.5%. Positive rate of malignancy by brush between 2007 and 2009 was 87.7% (50/57), while that between 2004 and 2006 was 71.8%, which were significantly different ( P ＜ 0. 05 ). The positive rate was not correlated with stricture location or tumor type. No major complications occurred, except for 4 moderate acute pancreatitis, 3 cholangiolitis and 2 biliary bleeding. Conclusion Brush cytology is of high sensitivity and specificity for malignant biliary stricture.Repeated brushing and manipulation can facilitate diagnosis yield.

Objective To investigate genetic characteristics of Neisseria gonorrhoeae (N.gonorrhoeae) isolates from Guangzhou city in 2014,and to analyze the relationship of N.gonorrhoeae multi-antigen sequence typing (NGMAST) sequence types (STs) with ciprofloxacin resistance.Methods An agar dilution method was used to determine the minimal inhibitory concentration (MIC) of ciprofloxacin in 97 N.gonorrhoeae isolates from Guangzhou city.PCR was performed to amplify the gyrA,parC,porB and tbpB genes from these isolates,followed by gene sequencing and determination of NG-MAST STs.Results Of the 97 N.gonorrhoeae isolates,95 (97.9％) were resistant to ciprofloxacin,including 41 high-level (MIC ≥ 16 mg/L) and 54 low-level (1 mg/L ≤ MIC ＜ 16 mg/L) resistant strains.Mutations were detected at codons 91 and 95 encoding serine in the gyrA gene of all the 95 ciprofloxacin-resistant strains,and in the parC gene of 93 resistant strains.The frequency of the mutation at codon 87 in the parC gene was 85.4％ (35/41) in high-level resistant strains,significantly higher than that in low-level resistant strains (59.3％[32/54],x2 =7.64,P ＜ 0.05).MAST STs were successfully determined for all the 97 N.gonorrhoeae isolates except 1 isolate with incorrect PCR amplicons.Of the 96 genotyped isolates,50 were assigned to 35 known STs by using the NG-MAST website (www.ngmast.net),among which,10 STs each contained 2 to 4 isolates.The most common ST was ST5309.Phylogenetic tree analysis revealed that the 96 genotyped N.gonorrhoeae isolates could be classified into 2 groups,and the proportion of isolates with MIC ≥ 16 mg/L is 46.4％ (39/84) in group 1,but only 1/12 in group 2 (x2 =6.27,P=0.012).Conclusions High-level resistance of N.gonorrhoeae to ciprofloxacin may be mainly associated with the mutation at codon 87 in the parC gene.NG-MAST STs may be related to the degree of ciprofloxacin resistance.