1.Determination of Puerarin Content in Jingning Capsule by HPLC
Ruju XIONG ; Xinfei MA ; Tulin LU
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(02):-
Objective To establish a method for determination of puerarin content in Jingning capsule. Methods The column was Kromasil (250 mm?4. 6 mm, 5 ?m). The mobile phase consisted of methanol- 0.2% water H3PO4 (20/80), with flow rate of 1 mL/min and the UV detective wavelength set at 250 nm. Results Puerarin showed a good linear at the range of 6.268~125.360 ?g/mL (r=0.999 8). The average recovery was 101.42%, with RSD of 1.22%. Conclusion This method is accurate, simple, repeatable and suitable for the determination of puerarin content in Jingning capsule.
2.Optimization of the Extraction Technology for Gengniankang Granules with the Multiple Guidelines Grading Method
Ruju XIONG ; Chunqin MAO ; Xuanzhong TAN
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(04):-
Objective To optimize the extraction technology for Gengniankang Granules.Methods An orthogonal test was adopted in the study.The contents of Paeoniflorin and Astragaloside Ⅳ were used as indices of appraisal.Data processing was carried out with the multiple guidelines grading method for optimizing the extraction condition.Results The optimized extraction technology for Gengniankang Granules was shown to be:eighteen fold volume of 70% alcohol should be added to 1 volume of the granules for 3 successive reflux extractions with 1.5 h of each.Conclusion The optimized extraction technology was rational and the extraction rate of the active components was high.