OBJECTIVE: To establish the method for the determination of emodin, resveratrol, polydatin and gentiopicrin in the Compound Polygonum cuspidatum semisolid matrix capsule in order to provide basis of its quality control. METHODS: TLC scanning method was used to determine emodin and resveratrol at same plate and the developer was petroleum ether-butyl acetate-methanol-glacial acetic acid(4∶1∶0.7∶0.03) with determination wavelength of 285 nm and 293 nm respectively. Polydatin and gentiopicrin were determined at same plate and the developer was chloroform-methanol(7 ∶ 2)with determination wavelength of 313 nm and 270 nm respectively. RESULTS: All target components were separated and the standard curves of emodin, resveratrol, polydatin and gentiopicrin showed good linearity. Average recoveries of them were 100.1%(RSD=0.86%),99.6%(RSD=2.86%), 101.4%(RSD=0.99%),98.5%(RSD=2.89%),respectively. CONCLUSION: Established method is rapid, simple for the quality control of Compound P. cuspidatum semisolid matrix capsule.

AIM: To establish the method of determining ginsenoside Rg1 and notoginsenoside R1,and control the quality of Yiqi Gushen Dialysis Liquid(Radix et Rhizoma Notoginseng,Radix Astragali,etc.) METHODS: Sample was pretreated with solid phase extraction using ODS C_13 cartridges.Ginsenoside Rg1 and Notoginsenoside R1 were separated with TLC method.The mobile phase was chloroform-methanol-water(3∶0.8∶0.1),developed twice.The coloration was 10% sulphuric acid ethanol solution.The detection wavelength was 560 nm. RESULTS: The calibration curves for ginsenoside Rg1 and notoginsenoside R1 were A=113.4 C-34.976,(r=0.997),A=92.14 C-13.597,(r=0.997),respectively,the linear ranges were between 2.64-13.2 mg and 0.85-8.5 mg,and the detection limit was 0.88 mg and 0.65 mg,respectively.The average recoveries were 100.31% and 98.72%,respectively.The contents of the sample were between 5.683-5.764mg,1.528-1.599 mg per 10 mL,respectively. CONCLUSION: The proposed method for estimating ginsenoside Rg1 and notoginsenoside R1 is simple,precise,specific,sensitive and accurate and can be used for quality control of Yiqi Gushen Dialysis Liquid.

OBJECTIVE:To establish a HPLC method for determination of anthraquinone free derivatives from Chinese herbal in dog serum METHODS:With Hypersil BDS C18 column,the mobile phase was methanol-0 5% acetic acid(80∶20) The sample was extracted with methanol and dried at room temperature The residue was reconstituted by methanol and analyzed at 430nm RESULTS:The five components can be separated well The linear ranges were Aloe-emodin(0 15～6 0?g/ml,r=0 9 996) Rhein(0 14～5 6?g/ml,r=0 9 982);Emodin(0 13～5 2?g/ml,r=0 9 990);Chrysophanol(0 2～8 0?g/ml,r=0 9 991);Physcion(0 1～4 0?g/ml,r=0 9 994) CONCLUSION:This method can provide the basis for vivo analysis of anthraquinone free derivatives and bioavailability study of Rheum palmatum preparations

OBJECTIVE:To optimize the extraction craft of gentiopicrin from gentian.METHODS:The extraction craft was optimized by orthogonal test with gentiopicrin as an index,and the content of gentiopicroside was determined by TLC scanning method.RESTLUTS:The quantity of menstruum and the extraction times of gentiopicrin had significant influence in the gentiopicrin extraction(P

OBJECTIVE: To optimize the extraction conditions of plumula nelumbinis. METHODS: The semi - bionic extraction (SBE) conditions were optimized by homogeneous design while total alkaloids and dry extract were adopted as markers. Factors of extraction time, solute quantity and the pH values were evaluated for their effects on extraction process. RESULTS: Optimal parameters were as follows: plumula nelumbinis was extracted in water 8 times as much as it for 3 times; the pH values of the water for the 1st, 2nd and 3rd decoctions were 5.0,6.0 and 8.0, respectively; the boiling lasted 4 hours. CONCLUSION: The method prouides theoretical reference for the extraction plumula nelumbinis.

Object To study the difference of phenylethanoid glycoside between the cultured cells of Cistanche salsa (C. A. Mey.) Benth. et Hook. f. and Herba Cistanche. Methods Making comparison by means of FTIR and HPLC technique. Results Using the FTIR technique, the infrared spectrum of cutured cell of C. salsa was similar to that of native Herba Cistanche at a certain extent. The HPLC analysis indicated that the main chemical constituents in cultured cells of C. salsa were similar to that of native Herba Cistanche while considerable difference of constituents was found between them. Conclusion On the whole, there are much more constituents in the cultured cells of C. salsa comparing with the native Herba Cistanche. The content of echinacoside and acteoside in the cultured cells is much higher than that in the native Herba Cistanche.

Objective To investigate the accuracy of the new trauma scoring method:Glasgow coma score (GCS),age and systolic pressure score (GAP),in diagnosing patients' traumatic condition and predicting fatality rate in department of emergency.Methods The chnical data of 27 706 traumatic patients in department of emergency were collected.The observation was categorized into three kinds:death within 24 h after reaching department of emergency (24 h death);death from 24 h after reaching department of emergency to 7 d (death after 24 h);survival.On the basis of the trauma mechanism,GCS,age and systolic pressure score (MGAP),the GAP method was used for judging traumatic condition and predicting fatality rate of traumatic patients in department of emergency.The accuracy of GAP in predicting fatality rate was compared with revised trauma score (RTS),trauma and injury severity score (TRISS) and MGAP.The C-statistics method was used to calculate the accuracy in predicted the fatality rate.Results The C values (0.936 and 0.961) of GAP in predicting fatality rate after 24 h and in 24 h were higher than RTS (0.917 and 0.958) and MGAP (0.920 and 0.950),but slightly lower than TRISS (0.950 and 0.970).The traumatic condition in GAP was defined in this way:severity 3-10 scores,moderation 11-18 scores,and slightness 19-24 scores.Conclusions The GAP is simple,practical and accurate in diagnosing patients' traumatic condition and predicting their fatality rate.It is helpful in physicians' decision of a proper treatment plan.

Objective To compare the pharmacokinetics difference of methotrexate ( MTX) alone or MTX combined with the decoction of Radix Salviae Miltiorrhizae ( RSM) in rats, and to investigate the possible impact of RSM on the efficacy and pharmacokinetics of MTX after oral administration. Methods Sprague-Dawley rats were given MTX (7 mg/kg) alone or MTX together with RSM (3.085 g/kg) respectively. At different time points, blood was sampled from orbital venous plexus and then was precipitated by perchloric acid. The serum concentration of MTX was determined by using high performance liquid chromatography ( HPLC) method. Chromatographic separation was achieved on a reversed-phase Diamonsil C18 (2) column with the mobile phase of methanol-formic acid water solution (formic acid in volume fraction of 0.1%) in gradient elution. The column temperature was 27 ℃, flow rate was 1 mL/min, detection wavelength was 302 nm, and the injection volume was 10 μL. The results were analyzed by pharmacokinetic software DAS ( 2.1.1) by non-compartment model. Results The linearity of the calibration curve for MTX was good in the range of 0.097 6 ~ 12.5 mg/L, the detection limit was 0.097 6 mg/L and the recovery was in the range of 77.5% ~ 86.6%. Compared with MTX group, the peak-arriving time of MTX-RSM group was advanced, and the clearance of MTX was delayed. Area under concentration-time curve at 0-t (AUC(0-t)) was increased by 0.609 times, and AUC(0-∞) was increased by 0.786 times. Conclusion The decoction of RSM exerts an effect on promoting the absorption of MTX, delaying the clearance of MTX and enhancing the bioavailability of MTX in vivo.

AIM: To establish an effective and economical processing method for high quality of Calcinatum Alumen with microwave heating. METHODS: A certain amount of Alumen was placed into microwave oven and heated, and its weight was determined by electronic balance every one or five minutes. The quality (appearance, aqueous solubility, water content) of Calcined Alum by microwave was compared with those of products prepared by traditional method, far IR, and electric oven. Moreover, the stability of Calcined Alum in several kinds of package were investigated at room temperature. RESULTS: The microwave for the processing of Alumen has the advantage of rapidness, low cost, and less water content, and its product could be dissolved completely in water. CONCLUSION: Microwave is a practical technique for Alumen processing.

Phe) polymorphism and GD in Han population of Chongqing area.