1.Minimal incision hip replacement through the modified Hardinge approach
Junzu HU ; Ruiying WANG ; Xinde YAO
Chinese Journal of Minimally Invasive Surgery 2005;0(11):-
Objective To investigate the feasibility of hip replacement through a minimal incision via modified Hardinge approach.Methods Hip replacement was performed in 21 cases(22 hip joints) from July 2005 to March 2006.The operation was completed through a minimal incision via modified Hardinge approach,with the greater trochanter as the center.There were 12 cases of total hip replacement(13 hip joints) and 9 cases of bipolar femoral head replacement.A biological prosthesis was used in all the cases. Results The mean length of incision was 7.6 cm(range,6.0~9.0 cm),the mean operation time was 76 min(range,(45~)150 min),and the mean blood loss,370 ml(range,190~580 ml).No severe complications occurred.All the cases were followed for a mean of 38 weeks(range,22~53 weeks).Evaluation with the Harris hip score system showed excellent outcomes in 18 cases(19 hips) and good in 3 cases,with a rate of excellent or good results of 100%.Conclusions Minimal incision hip replacement through the modified Hardinge approach has advantages of minimal trauma,little blood loss,low complication rate,and quick recovery.
2.Preparation and Characterization of Polyclonal Antibody Against Xenopus PAPC
Ruiying HU ; Peng XU ; Yuelei CHEN ; Xin LOU ; Xiaoyan DING
Progress in Biochemistry and Biophysics 2007;34(2):222-228
Xenopus Paraxial Protocadherin (PAPC), which was initially identified in a screen for genes present in the Spemann organizer of Xenopus embryos, is required for gastrulation, somitogenesis and otic vesicle formation. In order to investigate its function in various developmental events, an antibody was prepared which could specifically recognize Xenopus PAPC. Glutathione S transferase (GST) expression system was used to express the fusion protein GST-PAPC. Rabbits were immunized with GST-PAPC Western blotting analysis of FL-PAPC transfected HEK 293T cells lysates, which could be specifically blocked by pre-adsorption of prokaryotic expressed GST-PAPC fusion protein. Furthermore, by using immunofluorescence analysis the polyclonal antibody recognized membrane-bound PAPC in FL-PAPC transfected 293T cells and Xenopus animal cap cells. By Western blotting analysis,the endogenous 150 ku PAPC protein was detected in Xenopus embryos using the anti-PAPC antibody. Take together it could be concluded that a polyclonal antibody specifically against Xenopus PAPC was developed.
3.Misdiagnosed allergic granulomatous vasculitis as bronchial asthma: 1 case and literature review
Ruiying WANG ; Jianying XU ; Lei WANG ; Hu LIU
Chinese Journal of General Practitioners 2014;13(12):1009-1011
The clinical characteristics of Churg-Strauss syndrome (CSS) were summarized to improve the understanding of disease and reduce its misdiagnostic rate.Report clinical data,treatment and follow-up of one confirmed case and review the domestic reports of CSS before 2013.Among 94 reported cases,most had initial respiratory symptoms and increased eosinophil was found in peripheral blood of 95.74% patients.
4.Effect of Zusanli and Guanyuan Electroacupuncture on Inflammatory Reaction in Sepsis Patients
Guang YANG ; Ruiying HU ; Ming CHEN ; Jian LI
Journal of Guangzhou University of Traditional Chinese Medicine 2015;(3):430-433
Objective To study the effect of Zusanli and Guanyuan electroacupuncture ( EA) on inflammatory reaction in patients with sepsis. Methods A prospective and randomized trial was carried out in 58 sepsis patients, and the patients were randomly divided into EA group and control group. Patients in both groups were given conventional western medicine treatment, and EA group received EA at acupoints of Zusanli and Guanyuan additionally. The hospitalization time in intensive care unit ( ICU) of the two groups was compared. The changes of serum lactic acid (LA) level and inflammatory indexes including white blood cell and neutrophil count, C- reactive protein (CRP), erythrocyte sedimentation rate (ESR) and procalcitonin (PCT) of the two groups were observed before and after treatment. Results The hospitalization time in ICU of EA group was less than that of the control group, and the difference was statistically significant ( P<0.05) . On the seventh day after treatment, the serum LA level of EA group was significantly lower than that of the control group ( P<0.05) . Inflammatory indexes of EA group were decreased after treatment compared with those before treatment ( P<0.05) , and on seventh day after treatment, the inflammatory indexes of EA group were significantly lower than those of the control group ( P<0.05) . Conclusion EA at acupoints of Zusanli and Guanyuan is effective for the treatment of sepsis through reducing the inflammatory reaction of sepsis patients.
5.Expression of p16 and p15 Proteins in Acute Lymphoblastic Leukemia of Children
Wanru HU ; Ruiying ZHANG ; Chengji GUO ; Li'E WANG ; Shuyan DU ;
Journal of China Medical University 2001;30(1):64-65,68
Objective:Our aim was to To clarify the roles of p16 and p15 proteins in the genesis of acute lymphoblastic leukemia(ALL).Methods:Twenty-three samples of ALL were studied by the method of indirect immunofluorescence.Flow cytometer was used to estimate the cellular fluorescent intensity to determine the levels of p16 and p15 proteins.Results:Negative expression for p16 protein was found in 10 of 23 samples,and 8 of 23 were p15 negative expression.Both kinds of proteins were abscent in 6 samples.2 of 3 cases of T-ALL were negative expression of p16,p15 protein.In non T-ALL,6 of 13 were negative expression for p16 protein,5 of 13 were p15 protein deficient.The expression rates of p16,p15 protein in high leukocyte group were lower than those of non-high leukocyte group(P<0.05).The expression rates of p16,p15 proteins in HR-ALL were lower than those of SR-ALL(P<0.05).Conclusion:The p16 and p15 proteins take part in the genesis of ALL.Negative expression of p16,p15 proteins might imply the poor clinical outcome.
6.Autoimmune research on nerve root injury induced by non-compressive nucleus pulposus protrusion
Weijie YUAN ; Ziwei YUAN ; Ruiying WANG ; Junzu HU
Chinese Journal of Immunology 2015;(4):545-547,550
Objective:To discuss the autoimmune response on the mechanism and clinical significance of nerve root injury induced by the non-compressive nucleus pulposus protrusion.Methods:Forty-eight female SD rats were randomly divided.Twenty-four female SD rats were recruited as model group,and the others as control.After ten days,twenty days and forty days,the pain threshold of left hind leg and the levels of TNF-α, CD4+, CD8+T cells were measured, the nerve root changes in morphology were observed by HE.Results:Compared with the control group,model group in ten days,twenty days,which left hind leg pain threshold,the proportion of CD4,CD8+T cell and the expression of TNF-αare significantly different.After forty days,these two groups have no statistical differ-ence.After ten days,lumbar nerve root cross-sectional myelin of model group was partial disintegrated.The worst damage happened in twenty days,and almost recovered to normal in forty days.Conclusion:T cell-mediated autoimmunity and TNF-αplay an important role to the nerve root injury in the early time of the non-compressive nucleus pulposus protrusion.
7.Study on a new urine analysis core module based on semi-reflection mirror.
Longcong CHEN ; Gaiqin LIU ; Nan HU ; Ruiying ZHANG ; Qifeng JIANG ; Bin GAO ; Xingliang XIONG
Journal of Biomedical Engineering 2014;31(6):1288-1293
A new urine analysis core module based on high performance 32-bit microprocessor and high precision color sensor was presented. A novel optical structure and a specific circuit were applied to improve measurement precision and temperature was used to compensate for results in this core module. The information of urine test peice, such as all original data and color RGB value, reflectivity, semi-quantitative level, etc. can be output. The results showed that the measuring precision was about 95% or above with ideal stability and reliability using this presented core module, which can be conveniently applied in various urine analyzers, and can greatly decrease the cost of urine analyzers in development and production.
Color
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Equipment Design
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Microcomputers
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Reproducibility of Results
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Temperature
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Urinalysis
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instrumentation
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methods
8.CHOP expression and its correlation with proliferative/apoptotic ratio in colorectal adenoma-carcinoma sequence under same genetic background
Yiqian LI ; Junrong CHEN ; Chujun LI ; Ruiying ZHAO ; Huiling YANG ; Yingyu ZHU ; Qinghong CAI ; Siyuan HU
Chinese Journal of Pathophysiology 2014;(6):1004-1009
AIM:To investigate the expression of C/EBP homologous protein (CHOP) and its correlation with proliferative/apoptotic ratio (PAR) in colorectal adenoma-carcinoma sequence under the same genetic background .ME-THODS:Four kinds of tissue samples under the same genetic background from 23 patients, including normal colorectal tissue, adenoma with low-grade intraepithelial neoplasia , adenoma with high-grade intraepithelial neoplasia and colorectal adenocarcinoma samples , were collected .TUNEL method and Ki-67 immunohistochemistry were applied to determine the PAR.The expression of CHOP was detected by immunohistochemistry SABC method .RESULTS: ( 1 ) Under the same genetic background , the level of CHOP expression is significantly higher in colorectal adenocarcinoma than that in the ade -noma with high-grade intraepithelial neoplasia , the adenoma with low-grade intraepithelial neoplasia and the normal muco-sa.The level of CHOP expression was significantly higher in the adenoma with high -grade intraepithelial neoplasia than that in the adenoma with low-grade intraepithelial neoplasia and the normal mucosa .The level of CHOP expression was signifi-cantly higher in the adenoma with low-grade intraepithelial neoplasia than that in normal mucosa .(2) Under the same ge-netic background , PAR was significantly higher in the colorectal adenocarcinoma than that in the adenoma with high -grade intraepithelial neoplasia , the adenoma with low-grade intraepithelial neoplasia and the normal mucosa .PAR was significant-ly higher in the adenoma with high-grade intraepithelial neoplasia than that in the adenoma with low-grade intraepithelial neoplasia and the normal mucosa .PAR was significantly higher in the adenoma with low-grade intraepithelial neoplasia than that in the normal mucosa.(3) CHOP levels were positively correlated with PAR in the adenoma with low-grade intraepi-thelial neoplasia , adenoma with high-grade intraepithelial neoplasia and colorectal adenocarcinoma .CONCLUSION:CHOP expression and PAR continuously increased and positively correlated along the adenoma -carcinoma sequence , indica-ting that endoplasmic reticulum stress mediates the carcinogenesis of colorectal adenomas .
9.Association between FCGR polymorphisms and the susceptibility to cryptococcosis in human immunodeficiency virus-uninfected patients
Xiuping HU ; Jiqin WU ; Liping ZHU ; Xuan WANG ; Bin XU ; Ruiying WANG ; Yahui CAO ; Xinhua WENG
Chinese Journal of Infectious Diseases 2012;(11):656-662
Objective To describe the distributions of FCGR polymorphisms in human immunodeficiency virus (HIV)-uninfected patients with cryptococcosis,and to investigate the association of FCGR polymorphisms with the susceptibility to cryptococcosis.Methods The distributions of the four functional polymorphisms,including FCGR2A 131H/R,FCGR3A 158F/V,FCGR3B NA1/NA2,and FCGR2B 232I/T were compared between 198 cryptococcosis patients and 190 healthy controls.The polymorphisms distribution patterns were also compared between patients with central nervous system (CNS) infection and those without CNS infection.Genotyping of eight single nucleotide polymorphism (SNP) in FCGR were performed by multiplex SNaPshot technology using DNA extracted from blood samples.The comparison between patients and controls was performed by chi square test or Fisher exact test.Results Compared to healthy controls,the frequency of FCGR2B 232I/I increased (65% vs 53%,x2 =4.27,P=0.039,OR=1.652,95%CI:1.02-2.67) and that of FCGR2B 232I/T decreased (27% vs 40%,x2 =5.77,P=0.016.OR=0.542,95%CI:0.33-0.90) in patients with cryptococcal meningitis.Among immunocompetent patients,the frequency of FCGR2B 232I/I was also over-presented (69% vs 53%,x2=4.53,P =0.033,OR=1.958,95%CI:1.05-3.66) and the FCGR2B 232I/T genotype was also less frequently observed (24% vs 40%,x2=5.14,P=0.023,OR=0.467,95%CI:0.24-0.91) compared to healthy controls.There were 117 cases with CNS infection and 81 non-CNS infection cases.The genotype of FCGR2A 131R/Rwas over-presented (19% vs 6%,x2 =6.48,P=0.011,OR=3.52,95%CI:1.27-9.73) and the FCGR2B 232I/T genotype was under-presented (27 % vs 46 %,x2 =7.56,P =0.006,OR=0.431,95%CI:0.24-0.79) in patients with CNS infection compared with those without CNS infection.Furthermore,the frequency of FCGR2B 232I/I genotypes increased (69% vs47%,x2 =5.47,P=0.019,OR=2.479,95%CI:1.15-5.34) and the frequency of FCGR2B 232I/T decreased (24% vs 51%,x2 =8.66,P=0.003,OR=0.307,95%CI:0.14-0.68) in immunocompetent patients with CNS infection compared with those without CNS infection.Conclusions FCGR2A 131H/R and FCGR2B 232I/T are associated with the susceptibility to cryptococcal CNS infection,which suggests that FcγRⅡA and FcγRⅡB may contribute to the pathogenesis of cryptococcosis.
10.Design and implementation of Internet Public Opinion Monitoring and Early Warning System for medical and health industry
Yingying YU ; Liren WU ; Tiantian HU ; Lingfei MA ; Ruiying ZHU ; Peiwu SHI
Chinese Journal of Medical Library and Information Science 2017;26(3):37-40,53
The Internet Public Opinion Monitoring and Early Warning System for medical and health industry was designed and implemented due to the frequent occurrence of Internet public opinion, which has all-directional data collecting and analyzing functions, including big data collection, near duplicate detection, spam filtration, key public opinion early warning, region identification and tendency analysis, and can thus provide evidence for relevant departments to take effective measures for the control of Internet public opinion.