Objective To evaluate the clinical application of disk diffusion method(ROSCO company of Denmark) for testing the susceptibility of candida species isolated from patients with cancer with the using broth microdilution method ((NCCLS) M27-A of America Committee for clinical laboratory standards) as the gold standard in order to find the better method for clinical laboratory.Methods Antifungal susceptibility (5-flucytosine,amphotericin B,fluconazole and itraconazole) of 78 strains of Candida species were tested both by ROSCO disk diffusion method and ATB FUNGUR2 broth microdilution method.The microdilution method NCCLS as the gold standard.The sensitivity,specificity,positive and negative predictive values of two methods was calculated.Results The Kappa value was reached to 0.89 in term of disk diffusion method for detecting susceptibility of candida to 5-flucytosine,amphotericin B,fluconazole and itraconazole,suggesting that no serious errors was seen in terms of sensitive and resistant another method.Of 78 strains of candida,they had the high susceptibility to 5-flucytosine,amphotericin B,which were 88.20％ and 89.17 ％.The lower susceptibility of 78 strains of candida was fluconazole and itraconazole (56.34％ and 52.12％).Candida glabrata and candida krusei were proved to suspect to four kinds of antifungal agents(90.95％,85.71％).And the lowest positivity was 67.50％ and 41.67％ regarding of candida glabrata and candida krusei.Conclusion The higher consistency was seen in terms of detecting candida susceptibility to antibiotics by disk diffusion method and microdilution method,suggesting disk diffusion method can replace microdilution method.In bur hospital,candida species are susceptibility to amphotericin B at the highest sensitivity and courtyard albicans at lower sensitivity.Antifungal agents works to candida albicans and lower inhibition rate to candida kruse.

Objective To evaluate the effect of low-dose omeprazole in the treatment of functional dyspepsia(FD).Methods A total of 260 FD patients were diagnosed clearly and divided into two groups randomly:148 cases(observation group)were treated with low-dose omeprazole(10 mg/d),while 112 cases(control group)were treated with esomeprazole(20 mg/d)and domperidone(each 10 mg,three times per day).Two groups were all treated for 2 weeks.The clinical symptoms such as heartburn,postprandial distenstion,epigastric pain and eructation were observed.Results Compared with symptoms in two groups before treatment,the scores of heartburn and epigastric pain were decreased evidently after treatment in two groups(P

ObjectiveTo investigate the characteristics of the ambulatory blood pressure of acute cerebral infarction patients in the first 24 hours from onset and to study the relationship between their neurological deficit and characteristics of blood pressure.MethodsBlood pressure in 31 acute cerebral infarction patients (within 24 h) was measured serially during the first 24 hours in hospital with ambulatory blood pressure monitoring. Their neurological deficit was assessed with the National Institutes of Health stroke scale (NIHSS) on the first and the fifth day. Results83.87% nocturnal blood pressures decreased less than 10%. In multiple linear regression analysis, 24 h diastolic blood pressure (DBP) becomes the only and the most important factor to NIHSS on the first day. The patients with improved NIHSS on the fifth day showed lower nocturnal DBP and mean arterial pressure (MAP) than those of patients with unimproved NIHSS.ConclusionNot only the level but also the circadian of ambulatory blood pressure in acute cerebral infarction patients is significantly abnormal, especially for the reduction of nocturnal blood pressure decrease. On the first day, the neuroulogical deficit and blood pressure influence each other. The patients with higher nDBP and nMAP on the first day may be associated with poor early neurological function outcomes.

This article summarizes LI Shimao's academic view of tracing the origin and differentiating the pulse and syndrome in six aspects.1) Emphasizing pulse in diagnosis:he put forward the new theories of pulse diagnosis such as differentiating syndrome by pulse diagnosis outline theory,deficiency and excess pulse diagnosis outline theory and interpreting pulse mechanism with Qi and blood outline theory.2) Emphasizing sweat promotion method in the three methods (including sweat promotion,emetic method and purgative method):he promoted sweat with acrid-warm drugs not only in the treatment of excess cold syndrome,but also in the treatment of deficiency cold syndrome.3) Emphasizing Yang deficiency in cold damage syndrome:he regarded Yang deficiency as an important reason for the attack of externally-contracted wind-cold.4) Emphasizing fire constraint in warm disease:he was the first to pinpointed that the nature of warm disease is stagnated heat,he was good at treating warm disease with clearing,venting and enriching methods of which venting is the core and he invented Xinjia Shengjiang San (新加升降散) to treat stagnated heat;5) Emphasizing treating phlegm and stasis in the complicated diseases:he thought the difficult and complicated diseases in modem medicine were similar to the phlegm syndrome and stasis syndrome in Chinese medicine,so he liked modifying Daotan Tang (导痰汤),ZHANG Zhongjing's formulae,WANG Qingren's formulae for removing blood stasis and Taohong Siwu Tang (桃红四物汤) to treat phlegm and stasis syndromes;6) Emphasizing emergencies in rescue period:he thought Chinese medicine did a great work in treating emergencies,and modern scientific and technological methods should be used in studying emergencies in Chinese medicine.

Application of a number of philosophical ideas is instructive in eliminating the opposition on the doctors'right to inform and the patients right to being informed,building a harmonious relations between doctors and patients and correctly understanding and carrying out the informed consent.

Objective To explore the occupational stressors and the coping strategies in the newly-employed nurses and to explore their relationships. Method The survey was done among 193 registered nurses with less than 3 years working experience using the occupational stressor scale for Chinese nurses, the simplified coping strategies questionnaire and a self-designed general demography form. Results The total score on occupational stressors was 2.27 ± 0.56, which was at a common level. Postive and negative coping style were 1.88 ± 0.44 and 1.04 ± 0.51. The stressors together with the dimensions were positively correlated with negative coping strategies (P<0.05). Conclusion Nursing administration should improve work environment, provide enough manpower for patient care, carry out training system to focus on education for newly employed nurses, and provide advice and help for them when they need.

Objective To develop a precise and reliable method to determine mammalian cell survival using the triphe-nyltetrazolium chloride (TTC) solution.Methods Different amounts of TTC solution were added to the cultured cells in order to find the optimal concentration .The correlation between the cell number and the measured optic density value was analyzed.In addition, the TTC method was compared with traditional MTT assay .Results The TTC assay could precisely reveal the viability of cultured cells as well as the MTT method , but the non-specific reaction of the MTT reagent by other antioxidants could result in unreliable overestimation of the cell number .Conclusion The TTC assay can be used to deter-mine the survival of cultured cells more precisely .

Objective To provide reference for clinical application of combined antoxidants , by evaluating the com-bined and individual antioxidant activities of three commonly used antioxidants :edaravone,glutathion(GSH) and vitamin C ( VC) .Methods The multiple and individual antioxidant activities of the three antioxidants were compared by determining total antioxidant activity assay , reductive activity assay , superoxide anion scavenging assay and hydroxyl radical scavenging assay.Results and Conclusion Results showed that the combined antioxidants acted synergistically in scavenging free radicals and increased remarkably antioxidant activity at the same molar concentration .The rationality of different antioxi-dant combination should be taken into consideration in subsequent clinical application .

Objective To explore the community elderly depression effect on quality of life and provide the basis for the elderly mental health intervention. Methods Adopting stratified random sampling method, using the SF-12,CES-D and psychological capital questiennaire of elder to survey among 500 community elderly people and established the structural equation model. Results The age, degree of education, income situation, depression and the old psychological capital of the community elderly can be used to predict the quality of life, can explain 88.6%variance of life quality(P<0.05);depression on quality of life has a direct effect of-0.21(P < 0.05); depression through psychological capital indirect effect on quality of life for-0.64(P<0.05). Conclusions The elderly depression through psychological capital intermediary effect on the quality of life.