1.Health Risk Assessment of Nonylphenol in Reclaimed Wastewater
Ruixia HAO ; Yuwen ZHOU ; Ning YAO
Journal of Environment and Health 1992;0(05):-
Objective To assess the risk of nonylphenol in reclaimed wastewater used in city to damage the human endocrine system and to present the data for making the related water quality standard. Methods Based on the monitored results of nonylphenol in the reclaimed wastewater from a reclaimed water plant in Beijing and referenced the phenol toxic parameter from integrated risk information system of the Unite State Environmental Protection Agency and the nonylphenol tested toxicity data from related literature, the risk assessment of nonylphenol on human health was conducted using four step health risk assessments when the sewage was reclaimed as a different approach to use. Results The endocrine disruption risk of nonylphenol in reclaimed wastewater on human health was about in a range of 10-8-10-10, which could even be ignored. Conclusion The risk of nonylphenol is acceptable to human health when the reclaimed wastewater were used for municipal, agricultural irrigation, urban lake supplement, then, nonylphenol as a human health risk factor can be ignored when making the standard of nonylphenol in reclaimed water.
2.Survey on smoking related knowledge and intention of quitting among smokers with diabetes mellitus
Ruixia LU ; Yanling FAN ; Hao WU
Chinese Journal of General Practitioners 2017;16(11):863-866
Objective To survey on smoking related knowledge and intention of quitting among smoking patients with diabetes mellitus .Methods A questionnaire survey on smoking related knowledge and intention of quitting was conducted among 146 smoking patients with type 2 diabetes mellitus ( T2DM) in Beijing Yangfangdian Community Health Service Center from September 2016 to October.The questionnaire included general information ( 6 items ) , smoking status ( 3 items ) , awareness of smoking hazards ( 11 items), smoking cessation (5 items).Of the 146 patients, 82 were face-to-face surveyed, and 64 were telephone surveyed.Among 141 respondents who completed the questionnaire , there were 135 males (95.7%) and 6 females (4.3%) aged 32-74 (58.9 ±7.9) years, 4 cases (2.8%) with primary school education,46 cases (32.6%) with junior high school,63 cases (44.7%) with secondary school or high school education , 28 cases (19.9%) with college degree or above .The duration of diabetes ranged from 1 to 35 years with an average of (7.7 ±5.9) years.There was 2 cases (1.4%) with 1-5 years of smoking , 2 cases (1.4%) with 6-10 years of smoking, 25 cases (17.8%) with 11-20 years of smoking, 112 cases (79.4%) with more than 20 years of smoking.Results 99.3% (140/141) of the participants knew that smoking could lead to chronic bronchitis and emphysema , 46.8% ( 66/141 ) thought that smoking could lead to diabetes, 51.1%(72/141) thought that smoking affected blood glucose control , 65.2%(92/141) knew that smoking increased the incidence of cardiovascular and cerebrovascular diseases .The intention of quitting smoking was significantly correlated with the awareness of above knowledge in smoking diabetic patients.Conclusion The awareness rate of smoking causing diabetes , blood glucose control and diabetic chronic complications is relatively low .The awareness of smoking related knowledge can significantly facilitate patients to quit smoking .
3.Determinants of childhood trauma among college students with left-behind experience
XU Bin, SONG Qian, ZHANG Jianhua, HAO Aiyun, MA Nan, ZHANG Ruixia, ZHAO Hongxiang, CHEN Wei
Chinese Journal of School Health 2020;41(6):889-892
Objective:
To explore determinants of childhood trauma among college students with left-behind experience, and to provide a reference for effective intervention among students with left-behind experience.
Methods:
A total of 2 468 students selected from 5 universities and 2 higher vocational colleges in tianjin by stratified cluster sampling method were investigated by self-compiled questionnaire and childhood trauma questionnaire.
Results:
The scores in emotional abuse, sexual abuse, emotional neglect, physical neglect and childhood trauma of students with left-behind experience were significantly higher than those without left-behind experience(t=3.01,3.13,3.24,2.27,3.60,P<0.05);parental separation times and the frequency of parental return had significant interaction effect on the total score of childhood trauma of students with left-behind experience (F=2.37, P<0.05);the gender had a significant major effect on the total score of childhood trauma of students with left-behind experience under the interaction with the place of origin, age at first separation,the cumulative time of leftbehind experiences and the frequency of parents contacting (F=4.49,5.23,5.93,5.11,P<0.05);the age of subjects when parents going out under the interaction with the place of origin, the gender, if only-child,parental separation times and the frequency of parental return;as well as the frequency of parents contacting under the interaction with the place of origin,the household registration, the gender, if only-child and the cumulative time of left-behind experiences also had significantly main effect(F=3.88,4.25,3.32,2.86,3.45;3.82,4.02,2.64,3.29,P<0.05).
Conclusion
It is necessary to attach great importance to demographic and context information regarding left-behind experiences,which lead to more specific and effective prevention and intervention strategy for individual with left-behind experiences.
4. Effect of CASC19 on proliferation, apoptosis and radiation sensitivity of cervical cancer cells by regulating miR-449b-5p expression
Yanjie LIU ; Ruixia GUO ; Liping HAN ; Hao GU ; Mingzhu LIU
Chinese Journal of Obstetrics and Gynecology 2020;55(1):36-44
Objective:
To investigate the effects of cancer susceptibility candidate gene 19 (CASC19) regulating the expression of microRNA-449b-5p (miR-449b-5p) on the proliferation, apoptosis and radiation sensitivity of cervical cancer cells.
Methods:
(1) HeLa cells of cervical cancer cell line were cultured. HeLa cells were irradiated with X-ray at different doses (0, 2, 4, 6, 8 Gy, respectively), then the expression level of CASC19 mRNA and miR-449b-5p were detected by real-time quantitative PCR. (2) HeLa cell proliferation, apoptosis, radiation sensitivity (expressed as a survival fraction) and its related protein expression included cyclin D1, cleaved-caspase-3, and histone variant H2AX (γ-H2AX) were examined after different treatment including silencing CASC19 expression, over-expressing miR-449b-5p, down-regulating miR-449b-5p and silencing CASC19 expression. (3) The dual luciferase reporter gene experiment and real-time quantitative PCR technology were used to verify the targeting relationship between CASC19 and miR-449b-5p.
Results:
(1) With the increase of X-ray irradiation different dose (0, 2, 4, 6, 8 Gy), the expression level of CASC19 mRNA in HeLa cells gradually increased (