Objective To investigate the efficacy and safety of ozonated water injection for inflammatory pain of ankylosing spondylitis.Methods Forty patients suffered from inflammatory pain of ankylosing spondylit were admitted at the Xishan Coal Electricity Group Co.,Ltd Workers' General hospital from October 2013 to October 2016,and were randomly divided into Group A and Group B,20 cases in each group.Patients in Group A were treated with ozonated water injection into facet joint and sacroiliac joint,while patients in Group B were treated with diclofenac sodium controlled release table as control.Immunosuppressant was taken by all patients in both groups as the basic therapy.The VAS,ratio of VAS less than 3,Schober,Bath ankylosing spondylitis disease activity index(BASDAI),CRP,ESR and side effects were evaluated before treatment and 1 week,1 and 2 months after treatment.Results Before treatment,1st week,1st and 2nd month after treatment,the VAS in group A were (8.20 ± 0.92) points,(2.60 ± 0.79) points,(2.30 ± 0.67) points,(1.50 ± 0.53) points respectively,the Schober were (2.55± 1.62) cm,(3.10± 1.59) cm,(3.35±1.99) cm,(4.10t 1.37) cm,and the BASDAI were (6.30±0.64),(2.68±0.46),(2.08±0.40),(1.56±0.48).Before treatment,1st week,1st and 2nd month after treatment,the VAS in group B were (8.30± 1.06) points,(4.00± 1.05) points,(2.50± 1.18) points,(1.80± 1.03) points,the Schober were (2.50 ± 1.80) cm,(2.85± 1.49) cm,(3.95 ± 1.19)cm,(4.05±0.89) cm,and the BASDAI were (5.64±0.47),(2.98±0.29),(2.20±0.37),(1.70±0.45).The repeated measured repeated measures analysis of variance showed that among three indexes of VAS,Schober and BASDAI,the difference within group was statistically significant (P ＜ 0.05),but there were no significant difference between groups and interaction (P ＞ 0.05).The VAS and BASDAI of patients in both groups were significantly lower at 1st week,1st and 2nd month after treatment as compared to those before treatment,and the Schober of patients in group A at 1st week,1st month,2nd month and that of patients in group B at 1st month,2nd month significantly higher as compared with that before treatment(P＜0.05).The ratio of VAS less than 3 of patients in group A were 80％,100％ and 100％ at 1st week,1st and 2nd month after treatment,that in group B were 40％,80％ and 90％.The ratio of VAS less than 3 of patients in group A were significant higher than that of patients in group B at 1st week,1st month after treatment(P＜0.05).The CRP in group A were (43.3±12.6)mg/L,(8.46±6.92) mg/L before treatment,1st week after treatment,and that in group B were (43.1 ± 16.9)mg/L,(9.92±6.98) mg/L.The ESR in group A were (38.3±9.4) mm/h,(14.2±5.5) mm/h before treatment,1st week after treatment,and that in group B were (32.0± 10.1) mm/h,(12.8±6.5) mm/h.The CRP and ESR after treatment in two groups significantly lower as compared with those before treatment (P＜0.01),but there was no significant difference between group(P＞0.05).Four patients (20％) in group A and 11 patients(55％) in group B were suffered from gastrointestinal side effects after treatment,the ratio of group A was significant lower than that of group B (X2 =5.23,P＜ 0.05).Conclusion Ozonated water injection into facet joint and sacroiliac joint compound immunosuppressant improved pain and joint function,and exhibited a low gastrointestinal side effects.

Objective To find out the distribution of the subunit of Gs proteins (GNAS1) T393C polymorphism genetic in congestive heart failure patients and to investigate the effects of genetic polymorphisms on the therapeutic efficacy of carvedlol. Methods Genotype of 65 patients with congestive heart failure (heart function was ranked Ⅱ~Ⅳ level by NYHA) underwent PCR-RFLP for GNAS1 polymorphism genetic analysis, and then were treated with carvedlol. The initial dosage of carvedlol was 12.5 mg once daily in patients , and increased by 6.25 mg every 1 ~ 2 weeks for 12 months to a maximum dosage within 50 mg/d. Degree of LVEF changes and heart function improvement was adopted as measuring index , effect of Gs proteins α subunit T393C genetic polymorphism on carvedlol response in congestive heart failure patients was analyzed. Results After carvedlol treatment for 12 months , NYHA class distribution and LVEF in congestive heart failure patients improved significantly (P < 0.05). After carvedlol treatment for 6 months and 12 months mutation symptoms patients′ heart function improved significantly (P < 0.05) and LVEF increased significantly (P < 0.01). Conclusions GNAS1 T393C genetic polymorphism can influence the therapeutic efficacy of carvedlol in chronic congestive heart failure patients. 393 homozygous mutant and mutated symptoms patients′ heart improved more obviously and their LVEF improved more significantly.

Objective To observe the effects of happy family cognitive training on patients with vascular cognitive impairment. Meth-ods From April, 2015 to April, 2016, 80 patients with vascular cognitive impairment were divided randomly into happy family cognitive training group (n=40) and control group (n=40). Both groups accepted routine medicine and rehabilitation exercise, while the happy family cognitive training group received happy family cognitive training additionally. They were assessed with Montreal Cognitive Assessment (MoCA) and Activities of Daily Living (ADL) before and after 12 weeks of treatment. Results The scores of MoCA and ADL improved more in the family cognitive training group than in the control group after treatment (t>5.454, P<0.001). Conclusion Happy family cogni-tive training is effective on vascular cognitive impairment, for cognitive function and activities of daily living.

Objective To investigate the action and skills of camera-assisted laparoscopic rectal cancer radical surgery.Methods From January 2009 to May 2015,a total of 577 cases with rectal cancer underwent laparoscopic rectal cancer radical surgery,including 152 cases of abdominal perineal resection,and 425 cases of abdominal resection.The skills of camera-assisted operation were summarized.Results All the operations were successfully completed without serious complications.Conclusions Regarding for skills of camera assistant,mastering the clinical topography of rectum,understanding the operational habit of operator,understanding operating procedure and operation characteristics are the necessary condition to complete laparoscopic rectal cancer radical surgery.

Objective:To compare liver adhesive suspension method and V-shaped liver suspension of anterior abdominal wall in laparoscopic radical gastrectomy.Methods:This study retrospectively evaluated 115 patients who underwent laparoscopic radical gastrectomy from Jan 2018 to Dec 2019 at Shanxi Provincial People′s Hospital, 59 cases were under liver adhesive suspension (study group) and 56 cases under V-shaped liver suspension of anterior abdominal wall (control group). Intraoperative and postoperative conditions were observed and compared between the two groups.Results:All the 115 cases successfully completed surgery. The liver suspension time in study group was (2.4±0.5)min, which was significantly shorter than that of the control group((12.5±2.6) min)( t=-28.100, P<0.01). The total operation time in was (284±69) min and (307±78) min, respectively, ( t=-1.656, P=0.10). There were no statistically significant differences between the two groups in preoperative ALT, AST, total bilirubin, intraoperative incidence of hepatic lacerations and subcapsular hematoma, postoperative total bilirubin level, AST on the day 7 postoperatively, tumor location, TNM staging, surgical method, postoperative duration of hospital stay, and VAS score ( P>0.05). Conclusions:Both methods of liver suspension are safe and feasible in laparoscopic surgery for gastric cancer.Compared with V-shaped liver suspension of anterior abdominal wall, liver adhesive suspension has the advantages of less invasive, safe, and time-saving.

Objective: To investigate the therapeutic efficacy and potential mechanisms of fecal microbiota transplantation (FMT) in patients with type 2 diabetes mellitus (T2DM), and to preliminarily identify the traditional Chinese medicine (TCM) syndrome element characteristics of FMT in the treatment of T2DM. Methods: Between March 25, 2023 and September 30, 2024, T2DM patients who met the inclusion and exclusion criteria were enrolled at the Department of Rheumatology and Endocrinology of the Second Affiliated Hospital of Fujian University of Traditional Chinese Medicine. Participants received oral microbiota capsules as an adjunct to metformin therapy. Information obtained by four diagnostic methods of TCM, along with clinical and laboratory parameters, was collected before and after the intervention. Metagenomic sequencing was employed to analyze the gut microbiota, and Spearman correlation analysis was used to explore the relationship between laboratory indicators and differential bacterial genera. According to the post-treatment reduction in glycosylated hemoglobin (HbA1c), patients were categorized into a response (R) group and a non-response (NR) group. Treatment outcomes, safety indicators, gut microbiota changes, and TCM syndrome element features were compared between the two groups. Results: A total of 53 T2DM patients were included in the final analysis, and 30 patients were assigned to R group and 23 to NR group. After treatment, the R group exhibited significant reductions in HbA1c, fasting plasma glucose (FPG), and 2-hour postprandial glucose (2hPG) (P < 0.05 or P < 0.01). The NR group also showed significant decreases in HbA1c and FPG levels P < 0.01 or P < 0.05. Compared with the NR group, after treatment, FPG level in the R group demonstrated significant reductions (P < 0.01). As compared with before treatment, pancreatic islet function demonstrated enhancement in the R group, a significant increase in the 2-hour pastprandial C-peptide (2hC-P) levels in R group (P < 0.05), whereas no marked change was observed in the NR group. Regarding body composition indicators, the R group showed significantly lower waist-hip ratio (WHR), visceral fat (VF), and subcutaneous fat (SF) levels compared with the NR group (P < 0.01). After treatment, the NR group exhibited a significant elevation in aspartate aminotransferase (AST) levels (P < 0.05). Other safety-related indicators fluctuated within normal reference ranges, and no other adverse events, such as diarrhea, fever, or nausea, were reported. Metagenomic sequencing showed that FMT improved the diversity and richness of the gut microbiota, remodeling its overall structure. At the phylum level, the abundance of p_Firmicutes decreased significantly (P < 0.01), while the abundances of p_Bacteroidota and p_Proteobacteria increased significantly (P < 0.01). At the family level, among the 125 identified taxa, the abundances of f_Bacteroidaceae, f_Lactobacillaceae, and f_Sutterellaceae were significantly elevated, whereas six families, including f_Lachnospiraceae, f_Ruminococcaceae, and f_Coriobacteriaceae, were significantly decreased (all P < 0.05). Among the 367 taxa at the genus level, the top 10 differential genera showed significantly increased abundances of g_Bacteroides and g_Sutterella, and significantly decreased abundances in eight genera, including g_Faecalibacterium, g_Ruminococcus, g_Blautia, and g_Collinsella (all P < 0.05). Correlation analysis suggested that the phylum p_Bacillota was positively correlated with improvements in T2DM laboratory parameters, g_norank_f_Prevotellaceae was significantly positively correlated with fasting C-peptide (FC-P) and 2hC-P (P < 0.05). HbA1c demonstrated a significantly positive correlation with g_Blautia and g_Gemmiger (P < 0.05) and a significantly negative correlation with g_Bacteroides and g_Collinsella (P > 0.05). Analysis of syndrome element characteristics revealed that the R group was primarily characterized by pathological patterns of dampness, phlegm, and Yang deficiency. Before treatment, statistically significant reductions in syndrome element scores were observed for dampness, Yang deficiency, spleen, phlegm, Qi deficiency, Qi stagnation, and Yin deficiency (P < 0.01), as well as for heat and liver (P < 0.05). The NR group was mainly featured with Qi deficiency and Yin deficiency. Statistically significant changes in their syndrome element scores after treatment were noted for Qi deficiency (P < 0.01), and for spleen, Qi stagnation, liver, and blood deficiency (P < 0.05). In this group, the score changes for Yang deficiency, Yin deficiency, heat, and dampness were not statistically significant (P > 0.05). Conclusion The principles of syndrome element differentiation can be effectively applied to predict treatment efficacy and facilitate patient selection for FMT in the treatment of T2DM. Patients with T2DM presented with specific TCM syndrome element characteristics, notably dampness, phlegm, and Yang deficiency, represent a highly responsive population to FMT therapy.

Objective Transesophageal Doppler monitoring ( TDM) has been widely applied to fluid therapy in some major op-erations but rarely used in aortic valve replacement.The purpose of this study was to assess the value of TDM in fluid therapy, vasoactive drugs use, and left ventricular function in patients undergoing aorta valve replacement after cardiopulmonary bypass. Methods This study included 40 patients undergoing aotic valve replacement after cardiopulmonary bypass in Anhui Provincial Hospital from March 2014 to June 2015, which were randomly assigned to a TDM and a control group of equal number.TDM was used for the guidance of fluid infu-sion and vasoactive drug administration for the patients in the former group, while central venous pressure, mean arterial pressure, and heart rate were employed for those in the latter.The fluid volume infused, urine volume, blood loss, hemodynamics, arterial blood lac-tate, and outcomes were compared between the two groups. Results Compared with the control, the TDM group showed significantly increases in the colloidal fluid volume ([303 ±60] vs [373 ±65]mL, P<0.05), crystal fluid volume ([533 ±87] vs [596 ±83]mL, P<0.05), and urine volume ([274 ±95] vs [338 ±84]mL, P<0.05), but remarkable decreases in the red blood cell count, blood plasma vol-ume, postoperative extubation time, doses of dopamine and dobu-tamine, and length of hospital stay (all P<0.05).The hemodynamics were more stable and arterial blood lactate was lower in the TDM group than in the control (P<0.05). Conclusio n Transesophageal Doppler monitoring can timely and exactly manifest the hemodynamic changes and left ventricular function of the patient undergoing aotic valve replacement and provide precise guidance for fluid therapy and vasoactive drug administration.

This study was aimed to optimize the belt drying process conditions ofChuan-XiongConcrete. On the basis of the single factor experiment with drying temperature, feeding speed, drying time as three main variables and the transfer rate of ferulic acid as variable, the optimization of the belt drying process conditions ofChuan-XiongConcrete was identified using Box-Behnken Design. The results showed that the optimal drying parameters were as follows. The feeding speed was 1 kg·h-1. The drying temperature was 95℃. The drying time was 90 min. The transfer rate of ferulic acid was 95.48%. Three verification experiments were taken under these technological parameters. And the average transfer rate of ferulic acid was 95.71%, which was close to the predicted value of the model. It was concluded that the belt drying process of Chuan-Xiong Concrete was steady and feasible, which can be used in the drying process ofChuan-XiongConcrete.

Objective To investigate the expression of DNA binding protein A (dbpA) in patients with colorectal carcinoma of different stages and its significance.Methods Expression of dbpA protein and mRNA in specimens of normal tissues and colorectal cancer were detected by immunohistochemistry,expression of dbpA mRNA and protein of colorectal cancer cell lines SW480,RKO,SW620,DLD-1,HT-29,SW1463 and tissues were detected by immunohistochemistry,RT-PCR and Western blot.Results There was no positive staining dbpA protein and mRNA in normal colorectal tissues.However,dbpA was expressed in epithelial cells of colorectal mucosa [dbpA mRNA:80.0 ％ (48/60),10.0 ％ (6/60);dbpA protein:83.3 ％ (50/60),10.0 ％ (6/60),P ＜ 0.01].And there was no expression in normal colorectal cell,but its expression was high in 6 colorectal cancer cells (P ＜ 0.05).The high expression of dbpA was correlation with the infiltration depth,lymph node metastasis and type of histology (P ＜ 0.05),and had effect in prognosis of colorectal cancer (P ＜ 0.05).Conclusion Elevated dbpA may be related to the pathogenesis and development of colorectal carcinoma,and dbpA may be a prognostic factor of colorectal carcinoma.

Objective To study the efficacy and safety of Qingpeng ointment combined with oral Chinese Medicine in the treatment of plaque psoriasis.Methods By a randomized controlled method,60 patients with plaque psoriasis from December 2016 to October 2017 in Zhengzhou Hospital of Traditional Chinese Medicine were evenly divided into experimental group and control group.In the experimental group,patients were treated by Qingpeng ointment and oral Chinese Medicine,while in the control group,vaseline ointment and oral Chinese Medicine were provided.The period of treatment was 4 weeks.The PASI scores,itching index scores before and after treatment,clinical efficacy and security indices were compared between the two groups.Results The total effective rate was 93.33％ in the experimental group and 70.00％ in the control group,which displayed a statistical difference (P＜0.05).The PASI scores and itching index scores were significantly decreased in both groups after treatment (P＜0.05).The value of itching index in the experimental group was lower than that in the control group after treatment,with a statistical difference (P ＜0.05).No adverse reactions occurred in the patients in the two groups.Conclusions Qingpeng ointment combined with oral Chinese medicine is effective and safe in the treatment of plaque psoriasis.