Objective To observe the clinical efficacy of acupuncture in treating Oxycodone hydrochloride-induced constipation in cancer pain patients.Method Sixty cancer pain patients with Oxycodone hydrochloride-induced constipation were randomized into a treatment group and a control group, 30 cases in each group. The treatment group was intervened by acupuncture, while the control group was by oral administration of lactulose oral solution. The therapeutic efficacies were compared after 2-week treatment, and the quality of life was evaluated before and after treatment.Result The total effective rate was 90.0% in the treatment group versus 73.3% in the control group, and the difference was statistically significant (P<0.05). The Karnofsky Performance Status (KPS) score was significantly changed in the treatment group after intervention (P<0.05). There was a significant difference in comparing the KPS score between the two groups after treatment (P<0.05).Conclusion Acupuncture can produce a significant efficacy in treating Oxycodone hydrochloride-induced constipation, and also improve the patients’ quality of life.

Objective To develop an MR optical dual-modality probe targeting angiogenesis of gastric cancer and to study its physical characteristics , in vitro cytotoxicity and magnetic effects of different pulse sequences on 3 T clinical MR scanner.Methods We conjugated GX1-Cy5.5, a novel gastric cancer neo-vasculature targeted peptide labeled with Cy 5.5, to the surface functionalized magnetic nanoparticles according to different molecular weights (1∶100, 1∶500),resulting in dual-modality probe DPs100 and DPs500 (named DPs).The hydrodynamic size and zeta potential of DPs and DPs 500 were analyzed by nano-ZS.The human umbilical vein endothelial cells (HUVECs) and BGC-823 cells were treated with DPs for 24 h, and methyl thiazol tetrazolium ( MTT) method was used to detect the survival rate of cells.DPs with different concentrations were scanned on different MR sequences , and then the relative signal intensity was observed.The absorbance of HUVECs and BGC823 cells treated with DPs of different concentration (0.00, 1.25, 2.50, 15.00, 50.00, 100.00 and 150.00 μg/ml) were compared with single factor analysis of variance.Relative signal intensity of different MR sequences was compared using a paired Wilcoxon signed-rank test.Results The dual-modality probe targeting angiogenesis of gastric cancer was successfully constructed.The hydrodynamic size of iron oxide nanoparticles , DPs100 and DPs500 was (35.23 ±0.07), (39.49 ±0.16) and (40.43 ±1.70) nm and the Zeta potential was (0.31 ±0.20), ( -4.15 ±0.79) and ( -10.51 ± 2.37) mV.The coupled rates of DPs 100 and DPs500 with polypeptide were 92%and 94% respectively.The absorbance of HUVECs and BGC823 cells treated with DPs of different concentrations were 0.76 ±0.04, 0.80 ±0.03, 0.79 ±0.05, 0.75 ±0.06, 0.74 ±0.05, 0.77 ±0.01,0.71 ±0.04 and 0.38 ±0.04, 0.43 ±0.04, 0.41 ±0.03, 0.43 ±0.07, 0.44 ±0.04, 0.41 ±0.07 and 0.40 ±0.04, there was no statistical significance ( F=0.94, 0.51;P>0.05).The signal intensity increased first and then decreased following the increasing concentrations of DPs on T 1WI,especially on FSPGR T1WI (Z =-3.294,P <0.05), while the signal intensity decreased on T2WI or T2*WI.There was no significant differences in signal intensity on FSE T2 WI and SSFSE T2*WI with iron concentration >10μg/ml( Z=-7.110,P>0.05).With iron concentration≤10 μg/ml,the signal intensity on SSFSE T 2*WI was significantly decreased compared to FSE T2 WI ( Z =-2.023, P <0.05 ) .Conclusions DPs may be potential dual-modal probes for characterization of tumor angiogenesis by MR and optical imaging noninvasively , without causing significant effects on the cell activity in vitro , and SSFSE T2*WI may be the most sensitive sequence for DPs evaluation on MR.

Osimertinib is an irreversible third representative epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI) for the treatment of non-small cell lung cancer (NSCLC) with T790M resistance and classical EGFR mutations. However, the therapeutic effectiveness of osimertinib is limited by acquired drug-resistance, poor water solubility and low tumor accumulation rates. Nanodrug delivery systems can increase the solubility and stability of drugs, prolong the blood circulation time of drugs, improve the uptake rate of cells, promote drug accumulation in tumor tissues, and improve drug resistance. Thus, they are effective in overcoming the limitations of traditional targeted drugs. In this study, we reviewed the mechanism of action of the third-generation EGFR-TKI osimertinib, focused on research advances in osimertinib nanodrug delivery systems against NSCLC, and explored the challenges and future development direction in this field.

Childbirth is a physically demanding process. To improve the outcome of delivery and maternal satisfaction, active administering management becomes very important. This article reviews the importance, methods, and types of administering management of during the stage of delivery. It is recommended that low-risk puerpera not limited from oral intake during the stage of delivery. For those who fail to eat or dehydrate during labor, intravenous rehydration is recommended. Glucose normal saline is preferred as the type of fluid for rehydration.

Objective:To investigate the diet, dietary structure and physical activity of 408 early pregnant women in Beijing.Methods:From November 2018 to January 2019, this study selected 408 early pregnant women at the women and children hospital in Beijing by convenience sampling. The diet and physical activity were investigated with the Food Frequency Questionnaires (FFQ) and Denmark Physical Activity Questionnaire. SPSS 22.0 was used to build the database and principal component analysis was used to statistical analysis.Results:Among 408 early pregnant women, the gestational week and pre-pregnancy body mass index (BMI) was (9.77±1.95) weeks and (22.80±3.91) kg/m 2. There were 120 (30.9%) of pregnant women with pre-pregnancy overweight/fat, 136 (33.3%) of them with fasting blood glucose>4.6 mmol/L. Food consumption (cereals and tubers, livestock and poultry meat) all day was lower than recommended nutrient intake of the Chinese Balanced Diet Pagoda. Diet structure of pregnant women mainly included 5 kinds which explained 50.889% of the total cumulative variance; the first diet structures were the livestock and poultry meat, cereals, milk and milk products, tubers; and the vegetables, fried, barbecued, cookies and aquatic product were the second which explained 20.121% of the cumulative variance. Among those pregnant women, the time and energy consumption of resting or mild level of physical activity occupied 87.50% (21/24) and 81.50% (27.00/33.13) of time and energy consumption in 24 hours respectively; the time and energy consumption of medium level of physical activity occupied 7.29% (1.75/24) and 18.50% (16.13/33.13) of time and energy consumption in 24 hours respectively; level E (daily cleaning) accounted for 57.14% (1.00/1.75) and 48.93% (3.00/6.13) of the time and energy consumption of medium level of physical activity; there was no one with level H (aerobics) . Conclusions:Among 408 early pregnant women in Beijing, many pregnant women are with pre-pregnancy overweight/fat; a part of them are with a high level of fasting blood glucose; a part of them select diet outside. In the situation of low level of overall food consumption among early pregnant women, the diet structure mainly includes the livestock and poultry meat, grease, sweetmeats and tubers which leads to much sugar and fat intake. At the same time, early pregnant women have the low level of physical activity. Diet and exercise guide should be carried out to control the weight gain.

Objective:To explore the current status and influencing factors of feeling in control during labor in pregnant women with gestational diabetes mellitus (GDM) .Methods:Totally 200 pregnant women with GDM who underwent vaginal delivery in a Class Ⅲ Graded A maternity hospital in Beijing between from February to July 2018 were selected by convenient sampling. They were required to fill in the general information questionnaire, the Chinese version of Feeling in Control during Labor Scale (FCLS) and State Anxiety Inventory Form (S-AI) 48 hours after delivery to investigate their general information, feeling in control during labor and state anxiety. The factors affecting feeling in control during labor were analyzed, and the data of totally 172 pregnant women were collected.Results:The total score of FCLS in the 172 pregnant women with GDM was (148.00±23.03) , which was in a middle level. Univariate analysis showed that there were statistically significant differences in the scores of FCLS between pregnant women with GDM who had different education levels, occupations, the number of births, whether or not to undergo perineal lateral resection, and whether to use forceps ( P<0.05) . Multivariate analysis revealed that the whether to use forceps, the number of births, antenatal fasting blood glucose, and the level of anxiety during childbirth were the influencing factors for feeling in control of during labor in pregnant women with GDM. Conclusions:The level of feeling in control during labor in pregnant women with GDM in Beijing is higher than that of normal primiparous women. The in control during labor is affected by many factors, and clinical medical staff should take a variety of targeted measures for different populations to enhance their feeling in control during labor, thereby improving the delivery outcome and childbirth satisfaction.

Objective:To explore the knowledge-attitude-practice of pelvic floor muscle training in women with postpartum stress urinary incontinence (SUI) .Methods:From January 2018 to January 2019, we conveniently selected 213 patients in the Pelvic Floor Clinic of a Obstetrics and Gynecology Hospital in Beijing 12 weeks after delivery. We used the Pelvic Floor Muscle Function Training Knowledge-Attitude-Practice and Its Needs in Puerperium Women Questionnaire for investigation, and analyzed the influencing factors of knowledge-attitude-behavior of patients' pelvic floor muscle function training.Results:Among 213 SUI patients, the overall standard score of pelvic floor muscle training knowledge-attitude-practice was (75.70±10.23) . Univariate analysis showed that there were statistical differences in the knowledge-attitude-practice of pelvic floor muscle function training among patients with different age, family monthly income per capita, highest education level, occupational status, delivery method, weight gain during pregnancy, and current urinary incontinence ( P<0.2) .Multiple regression analysis showed that family monthly in comeper capita, weight gain during pregnancy, and current urinary incontinence were the influencing factors of pelvic floor muscle function training knowledge-attitude-practice with a statistical difference ( P<0.05) . Conclusions:Postpartum SUI patients have a positive attitude towards pelvic floor muscle function training, but their cognitive level needs to be improved. Medical and nursing staff should strengthen the publicity and education of relevant knowledge, and enhance patients' awareness of the importance of pelvic floor muscle function training.

Objective To summarize the outcomes and prognostic factors in esophageal cancer (EC) patients.Methods A total of 307 EC patients of stages Ⅱ-Ⅲ were treated with concurrent chemoradiotherapy in our hospital from September 2006 to July 2014.There were 73 patients with stage Ⅱ and 234 with stage Ⅲ.The radiotherapy dose was 50-70 Gy (median 60 Gy).Concurrent chemoradiotherapy were used with fluorouracil plus platinum (PF,166),paclitaxel plus platinum (TP,82) or platinum only (P,59).The Kaplan-Meier method was used to calculate overall survival (OS) and progression-free survival (PFS) rates,the log-rank test was used for survival difference analysis and univariate prognostic analysis.The Cox regression model was used for multivariate prognostic analysis.Results The 1-,3-5-year OS and PFS rates were85.6％,53.8％,36.9％ and 74.6％,43.7％,33.1％,respectively.The median OS and PFS were 41.6 months and 29.8 months.The univariate analysis indicated that T stage,N stage,clinical stage,lesion location,lesion length and chemotherapy regimen were prognostic factors for OS and PFS (P=0.007 and 0.013,0.000 and 0.000,0.000 and 0.000,0.002 and 0.000,0.141 and 0.005,0.018 and 0.165).Multivariate analysis showed that T stage,N stage,lesion location and chemotherapy regimen were prognostic factors for OS (P =0.024,0.000,0.007 and 0.028),lesion location,lesion length and N stage were prognostic factors for PFS (P=0.004,0.033 and 0.035).The median OS and PFS for EC patients treated by total dose 50-60 Gy,＞60-70 Gy were 47.4 months,37.8 months (P=0.469) and34.1 months,25.1 months (P=0.0.233),therewere no statistic difference.Conclusions The outcome of EC patients treated with concurrent chemoratherapy could obtain a long-term survival,combination chemotherapy is superior to single drug,there are no statistical difference between high-dose and low-dose,and the acute toxic effects can be tolerated.

Klebsiella oxytoca is an important opportunistic pathogen which cause community or hospital-acquired infections in adults and children. The disease it most causes is antibiotic-associated hemorrhagic colitis (AAHC). It can also cause diseases such as urinary tract infections, pneumonia and bloodstream infections. The cytotoxins including Tilivalline and Tilimycin are important virulence factors for Klebsiella oxytoca, mainly causing AAHC. This article reviewed the progress of research on the prevalence, pathogenicity and mechanisms of K.oxytoca, hoping to improve the understanding of K.oxytoca and provide guidance on disease prevention and treatment.

Ubiquitin specific protease 33 (USP33) is a multifunctional protein regulating diverse cellular processes. The expression and role of USP33 in lung cancer remain unexplored. In this study, we show that USP33 is down-regulated in multiple cohorts of lung cancer patients and that low expression of USP33 is associated with poor prognosis. USP33 mediates Slit-Robo signaling in lung cancer cell migration. Downregulation of USP33 reduces the protein stability of Robo1 in lung cancer cells, providing a previously unknown mechanism for USP33 function in mediating Slit activity in lung cancer cells. Taken together, USP33 is a new player in lung cancer that regulates Slit-Robo signaling. Our data suggest that USP33 may be a candidate tumor suppressor for lung cancer with potential as a prognostic marker.
Blotting, Western ; Cell Line, Tumor ; Cell Movement ; genetics ; physiology ; Cohort Studies ; Down-Regulation ; Female ; Gene Expression Regulation, Neoplastic ; HEK293 Cells ; Humans ; Immunohistochemistry ; Intercellular Signaling Peptides and Proteins ; metabolism ; Kaplan-Meier Estimate ; Lung Neoplasms ; genetics ; metabolism ; pathology ; Male ; Middle Aged ; Nerve Tissue Proteins ; metabolism ; Prognosis ; RNA Interference ; Receptors, Immunologic ; metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Signal Transduction ; genetics ; physiology ; Ubiquitin Thiolesterase ; genetics ; metabolism