AIM:TostudythefunctionofmicroRNA(miR)-19aandmiR-92abyseed-targetinginhibitionin multiple myeloma cells and their signal pathways .METHODS:The experiments were divided into t-antimiR-19a group, t-antimiR-92a group, scramble control group and blank control group .The growth-inhibitory potencies were measured by MTT assay.The ability of cell colony formation was measured by cell colony formation assay .The ability of cell invasion was measured by Transwell experiment .The miR-19a and miR-92a target gene signal pathways were integrated by miRFo-cus software.RESULTS:MTT assay showed that t-antimiR-19a and t-antimiR-92a significantly inhibited the viability of multiple myeloma cells , and the best concentration and time were 0.5μmol/L and 48 h, respectively .The colony number in t-antimiR-19a/92a group was less than that in scramble control group .The transfection with t-antimiR-19a or t-antimiR-92a effectively decreased the cell invasion , as the relative invasion cell number was significantly decreased compared with scramble control group.miR-19a and miR-92a were involved in mTOR signaling, cell cycle and other cancer pathways . CONCLUSION:miR-19a and miR-92a cluster might be a potential target for therapeutic intervention in multiple myelo-ma.

Childbirth is a physically demanding process. To improve the outcome of delivery and maternal satisfaction, active administering management becomes very important. This article reviews the importance, methods, and types of administering management of during the stage of delivery. It is recommended that low-risk puerpera not limited from oral intake during the stage of delivery. For those who fail to eat or dehydrate during labor, intravenous rehydration is recommended. Glucose normal saline is preferred as the type of fluid for rehydration.

Objective:To explore the knowledge-attitude-practice of pelvic floor muscle training in women with postpartum stress urinary incontinence (SUI) .Methods:From January 2018 to January 2019, we conveniently selected 213 patients in the Pelvic Floor Clinic of a Obstetrics and Gynecology Hospital in Beijing 12 weeks after delivery. We used the Pelvic Floor Muscle Function Training Knowledge-Attitude-Practice and Its Needs in Puerperium Women Questionnaire for investigation, and analyzed the influencing factors of knowledge-attitude-behavior of patients' pelvic floor muscle function training.Results:Among 213 SUI patients, the overall standard score of pelvic floor muscle training knowledge-attitude-practice was (75.70±10.23) . Univariate analysis showed that there were statistical differences in the knowledge-attitude-practice of pelvic floor muscle function training among patients with different age, family monthly income per capita, highest education level, occupational status, delivery method, weight gain during pregnancy, and current urinary incontinence ( P<0.2) .Multiple regression analysis showed that family monthly in comeper capita, weight gain during pregnancy, and current urinary incontinence were the influencing factors of pelvic floor muscle function training knowledge-attitude-practice with a statistical difference ( P<0.05) . Conclusions:Postpartum SUI patients have a positive attitude towards pelvic floor muscle function training, but their cognitive level needs to be improved. Medical and nursing staff should strengthen the publicity and education of relevant knowledge, and enhance patients' awareness of the importance of pelvic floor muscle function training.

Objective:To investigate the diet, dietary structure and physical activity of 408 early pregnant women in Beijing.Methods:From November 2018 to January 2019, this study selected 408 early pregnant women at the women and children hospital in Beijing by convenience sampling. The diet and physical activity were investigated with the Food Frequency Questionnaires (FFQ) and Denmark Physical Activity Questionnaire. SPSS 22.0 was used to build the database and principal component analysis was used to statistical analysis.Results:Among 408 early pregnant women, the gestational week and pre-pregnancy body mass index (BMI) was (9.77±1.95) weeks and (22.80±3.91) kg/m 2. There were 120 (30.9%) of pregnant women with pre-pregnancy overweight/fat, 136 (33.3%) of them with fasting blood glucose>4.6 mmol/L. Food consumption (cereals and tubers, livestock and poultry meat) all day was lower than recommended nutrient intake of the Chinese Balanced Diet Pagoda. Diet structure of pregnant women mainly included 5 kinds which explained 50.889% of the total cumulative variance; the first diet structures were the livestock and poultry meat, cereals, milk and milk products, tubers; and the vegetables, fried, barbecued, cookies and aquatic product were the second which explained 20.121% of the cumulative variance. Among those pregnant women, the time and energy consumption of resting or mild level of physical activity occupied 87.50% (21/24) and 81.50% (27.00/33.13) of time and energy consumption in 24 hours respectively; the time and energy consumption of medium level of physical activity occupied 7.29% (1.75/24) and 18.50% (16.13/33.13) of time and energy consumption in 24 hours respectively; level E (daily cleaning) accounted for 57.14% (1.00/1.75) and 48.93% (3.00/6.13) of the time and energy consumption of medium level of physical activity; there was no one with level H (aerobics) . Conclusions:Among 408 early pregnant women in Beijing, many pregnant women are with pre-pregnancy overweight/fat; a part of them are with a high level of fasting blood glucose; a part of them select diet outside. In the situation of low level of overall food consumption among early pregnant women, the diet structure mainly includes the livestock and poultry meat, grease, sweetmeats and tubers which leads to much sugar and fat intake. At the same time, early pregnant women have the low level of physical activity. Diet and exercise guide should be carried out to control the weight gain.

Objective:To explore the current status and influencing factors of feeling in control during labor in pregnant women with gestational diabetes mellitus (GDM) .Methods:Totally 200 pregnant women with GDM who underwent vaginal delivery in a Class Ⅲ Graded A maternity hospital in Beijing between from February to July 2018 were selected by convenient sampling. They were required to fill in the general information questionnaire, the Chinese version of Feeling in Control during Labor Scale (FCLS) and State Anxiety Inventory Form (S-AI) 48 hours after delivery to investigate their general information, feeling in control during labor and state anxiety. The factors affecting feeling in control during labor were analyzed, and the data of totally 172 pregnant women were collected.Results:The total score of FCLS in the 172 pregnant women with GDM was (148.00±23.03) , which was in a middle level. Univariate analysis showed that there were statistically significant differences in the scores of FCLS between pregnant women with GDM who had different education levels, occupations, the number of births, whether or not to undergo perineal lateral resection, and whether to use forceps ( P<0.05) . Multivariate analysis revealed that the whether to use forceps, the number of births, antenatal fasting blood glucose, and the level of anxiety during childbirth were the influencing factors for feeling in control of during labor in pregnant women with GDM. Conclusions:The level of feeling in control during labor in pregnant women with GDM in Beijing is higher than that of normal primiparous women. The in control during labor is affected by many factors, and clinical medical staff should take a variety of targeted measures for different populations to enhance their feeling in control during labor, thereby improving the delivery outcome and childbirth satisfaction.