Objective To investigate the main allergens in patients with allergic rhinitis in Zhengzhou district.Methods Twenty standardization allergens were used to perform skin prick test in 1 139 patients with allergic rhinitis.The samples were divided into four groups according to the age of the patients.The distributions of different allergens in the four groups were analyzed.Results The top five inhaled allergens were dermatophagoides farina (920 cases,80.8％),dermatophagoides pteronyssinus (870 cases,76.4％),cat hair (517 cases,45.4％),herbs (397 cases,34.9％) and poaceae (353 cases,31.0％) ; the top five ingestive ones were shrimp (143 cases,12.6％),peanut (66 cases,5.8％),egg (56 cases,4.9％),carp (51 cases,4.5％) and meat (48 cases,4.2％).The distribution of allergens was different among the different age groups.Conclusion Dust mites,cat hair,herbs and poaceae were the most common allergens in Zhengzhou district.

Objective:To investigate the expression of F-spondin (SPON1) in cervical cancer tissues and its effects on the biological behavior of Hela cell line, including proliferation, apoptosis, invasion, and migration.Method:Select 80 samples of cervical cancer tissue and 80 samples of cervical inflammation tissue stored in the Pathology Department of Xuzhou Medical University from January 2022 to May 2023. Immunohistochemistry was used to detect the expression of SPON1 in cervical cancer and chronic cervicitis tissues, and the corresponding clinicopathological data were collected for analysis. Small disturbance RNA(siRNA) was used to interfere with the expression of SPON1 in Hela cell lines. Untransfected cells were set as normal control group (Ctrl group), negative control group (NC siRNA group), and siRNA knockout group (SPON1 siRNA group). The interference effect was detected by qPCR and Western blot. Cell proliferation assay (CCK-8), flow cytometry, Transwell, and scratch assay were used to detect the proliferation, apoptosis, invasion, and migration of each group of cells. Each experiment was repeated three times. Quantitative data that conforms to a normal distribution are represented by xˉ± s. The comparison of means among the three groups was conducted using one-way analysis of variance; Compare pairwise using LSD- t test. Calculate the percentage based on qualitative data, and compare the rates between groups using the chi square test. P<0.05 indicates a statistically significant difference. Results:Compared with the chronic cervicitis group, the positive expression rate of SPON1 in cervical cancer tissues (72.5% (58/80)) was higher than that in chronic cervicitis tissues (20.0% (16/80)), and the difference was statistically significant ( χ2=44.35, P<0.001). The positive expression rate of SPON1 was positively correlated with the clinical stage, pathological differentiation and tumor diameter of cervical cancer ( χ2 values were 4.10, 4.98, and 4.40, respectively; P values were 0.043, 0.026, and 0.036, respectively), and were not related to the age and tissue type of patients ( χ2 values were 0.72 and 0.14, P values were 0.386 and 0.713, respectively). The relative expressions of SPON1 mRNA were 1.000±0.014, 0.966±0.082, and 0.365±0.036, respectively. The expression levels of SPON1 protein were 1.000±0.013, 1.022±0.031, and 0.655±0.026, respectively. The number of cells that penetrated the model in the three groups was (113.3±4.1), (107.0±3.1), and (80.3±3.2), respectively. The wound healing rates of the three groups were (56.00±3.45)%, (55.00±5.03)%, and (35.33±3.76)%, respectively, and the apoptosis rates of the three groups were (5.88±0.44)%, (6.27±0.38)%, and (10.50±0.39)%, respectively. Compared with the Ctrl group and the NC-siRNA group, the proliferation ability of cells in the siRNA interference group decreased (all P<0.001), invasion ( P values were 0.001 and 0.004) and migration ( P values were 0.029 and 0.035), and the apoptosis rate was increased ( P=0.001). Conclusion:SPON1 is highly expressed in cervical cancer tissue and is associated with the occurrence and development of cervical cancer. Inhibiting SPON1 expression significantly reduces the proliferation, invasion, and migration ability of Hela cells, and promotes their apoptosis.

Objective:To examine the survival rates and clinical characteristics of people with newly discovered non-M 3 acute myeloid leukemia (AML) who carry the ASXL1 gene mutation. Methods:From January 2016 to April 2021, the clinical information of patients with newly diagnosed non-M 3 AML at Shandong University's Qilu Hospital was retrospectively examined, and their clinical characteristics and survival were compared and analyzed. Gene mutation was detected by next-generation sequencing. Results:① The study included 256 AML patients who were initially diagnosed and had complete data, including 47 cases of ASXL1 gene mutation-positive (ASXL1 +) patients and 209 cases of ASXL1 gene mutation-negative (ASXL1 -) patients. All patients were divided into three groups: elderly (≥60 years old, n=92) , middle-aged (45-59 years old, n=92) , and young (≤44 years old, n=72) . ②WBC, and age were higher in patients with ASXL1 mutations compared to ASXL1 - patients, while complete response after the first round of treatment (CR 1) was lower ( P<0.05) . In the elderly group, WBC and the proportion of aberrant cells in nuclear cells in ASXL1 + patients were higher than those in ASXL1 - patients ( P<0.05) . In the young group, the WBC of ASXL1 + patients was higher than that of ASXL1 - patients ( z=-2.314, P=0.021) . ③IDH2 mutation and ASXL1 mutation was related ( P=0.018, r=0.34) . In ASXL1 + patients, the proportion of peripheral blasts in the high VAF group (VAF>40% ) was higher than that in the low VAF group (VAF<20% ) , and the proportion of aberrant nuclear cells was higher in the duplication and replacement mutation patients than in the deletion mutation patients ( P<0.05) . ④The overall survival (OS) and progression-free survival (PFS) of ASXL1 + patients were shorter than those of ASXL1 - patients (median, 10 months vs 20 months, 10 months vs 17 months; P<0.05) . The proportion number of aberrant cells in nuclear cells (≥20% ) , complex karyotypes, and TET2 mutation were all independent risk variables that had an impact on the prognosis of ASXL1 + patients, according to multivariate analysis ( P<0.05) . Conclusion:ASXL1-mutated non-M 3 AML patients have higher WBC in peripheral blood, a higher proportion of aberrant cells in nuclear cells, lower CR 1 rate, and shorter OS and PFS. Additionally, a poor prognosis is linked to higher VAF, duplication, and substitution mutations in the ASXL1 gene, as well as the high proportion of aberrant cells in nuclear cells, complex karyotype, and TET2 mutation.

OBJECTIVE: To classify Right Bundle Branch Block (RBBB),Left Bundle Branch Block (LBBB) and normal ECG signals automatically. METHODS: The MIT-BIH database was used as experimental data sources.The training set and test set were extracted for training and testing network models.Based on convolutional neural network,this paper proposed the core algorithm:sparse connection residual network.Compared the sparse connected residual network with classic network models,then evaluated the recognition effect of the model. RESULTS: The accuracy of the test set the MIT-BIH database was 95.2%,the result is better than classic network models. CONCLUSIONS The algorithm proposed in this paper can assist doctors in the diagnosis of heart block related disease and place a high value on clinical application.
Algorithms ; Arrhythmias, Cardiac ; diagnostic imaging ; Bundle-Branch Block ; diagnostic imaging ; Electrocardiography ; Humans ; Neural Networks (Computer)

Objective:To clarify the pathogen types, genotypes and molecular biological characteristics of an outbreak in a high school in Yantai city in 2019, and to provide evidence for epidemic prevention and control.Methods:Eleven samples were collected from a high school in Yantai city in 2019. Quantitative PCR was used for primary type identification. RT-PCR and specific primers were used to amplify the target genes, and the sequences of norovirus were compared and analyzed for phylogenetic analysis.Results:The pathogen of this cluster outbreak was norovirus. Five GII-positive samples of norovirus were detected, 4 of which were GII.13 and 1 was GII.17. Sequence analysis of polymerase-capsid region showed that the amino acid sequence of this cluster outbreak was highly conserved.Conclusions:This outbreak is a cluster of diarrhea caused by GII.13 and GII.17 norovirus infection. The analysis of this outbreak is helpful to our general understanding of the evolution, genetic diversity and distribution of norovirus, and the surveillance of norovirus in the jurisdiction should be further strengthened.

Objective:To investigate the pathogenic spectrum of enteroviruses associated with hand, foot and mouth disease (HFMD) in the Yantai region of Shandong province in 2016, and analyze the evolution of epidemic strains of coxsackie virus group A type 6 (CV-A6) in the pathogenic spectrum of HFMD enteroviruses and the variations of important amino acid sites in the VP1 region.Methods:A total of 738 samples were collected from the patients with HFMD in Yantai region in 2016 to conduct DNA and serotype tests of enterovirus (EV) by real-time RT-PCR and further count the number and proportion of each type of enterovirus positive specimens. Based on the predominant serotype of enteroviruses, eight serotypes of the CV-A6 strains were selected to carry out VP1 regions amplification for the determination and analysis of nucleotide sequencing and phylogenetic analysis.Results:A total of 460 enteroviruses strains were isolated from 738 samples, including pathogens strains: 258 CV-A16 (56.09%), 62 EV-A71 (13.48%), 49 CV-A10 (10.65%), 44 CV-A6 (9.57%) and 9 CV-A4 (1.96%). Eight CV-A6 positive specimens were isolated from the viruses and the nucleotide-sequence analysis of the whole VP1 region was conducted. The sequence analysis of eight CV-A6 strains demonstrated that the homologies of nucleotide and amino acid were 96.12% - 100% and 97.78% - 100% respectively. The phylogenetic analysis indicated that the eight CV-A6 strains were subdivided into the genotype D subtype D3. Compared with the reference strain, CVA6-Gdula-AY421764, amino acids of CV-A6 strains in Yantai city observed at sites 10, 14, 174, 194, 279, 283 and 305 in VP1 region appeared mutant.Conclusions:CV-A16, EV-A71, CV-A10 and CV-A6 were the main common pathogens of HFMD in Yantai region in 2016. All the CV-A6 strains isolated in this study belonged to subtype D3 in genotype D.

Objective: To systematically review the diagnostic accuracies of multiplex real time polymerase chain reaction (MRT-PCR) technique for detection of respiratory syncytial virus (RSV) and adenovirus (ADV). Methods: PubMed, EMBASE, Cochrane, Wanfang and CNKI databases were searched from January1 2010 to January1 2018, to collect reports on MRT-PCR for detection of common respiratory viruses. Then two authors independently exacted the data and assessed the risk of bias of included studies by using the QUADAS-2 tool. Meta-disc 1.4. Results: Ten articles with 2528 cases were eligible for analysis. The result of meta-analysis showed that, the pooled Sen, Spe and area under SROC curve, for detecting RSV were 0.87 (95% CI 0.83 to 0.90), 0.98 (95% CI 0.97 to 0.98) and 1.00. The pooled Sen, Spe, and area under SROC curve of MRT-PCR for detecting ADV were 0.64 (95% CI 0.56 to 0.71), 0.99 (95% CI 0.98 to 0.99) and 0.99. Deeks test indicated that no publication bias was found. Conclusions The sensitivity of MRT-PCR in RSV and ADV detection is still to be improved, but the overall detection ability is good which deserves to be recommended for clinical use.

Objective: To evaluate the residual radioactivity after ¹³¹I treatment in postoperative inpatients with differentiated thyroid carcinoma (DTC) using service robot in nuclear medicine ward, and assess the time for patients to be released from isolation. Methods: From September 2017 to June 2018, 297 patients (94 males, 203 females, age: 19-80 years) with DTC who underwent ¹³¹I treatment after surgery were included. According to the purpose of treatment and the prescription dosage of ¹³¹I, patients were divided into 8 groups: 4 groups accepted ¹³¹I remnant ablation therapy (RAT) with different dosages, which were 3 700 MBq (RAT1, n=34), 4 440 MBq (RAT2, n=122), 5 550 MBq (RAT3, n=81) and 7 400 MBq (RAT4, n=27), respectively; 4 groups had ¹³¹I treatment for recurrent/metastatic lesions (RMLT), and the dosages were 3 700 MBq (n=1), 4 440 MBq (n=2), 5 550 MBq (n=14) and 7 400 MBq (n=16). At 4, 24, 48 and 72 h after ¹³¹I administration, the dose equivalent rates at 2 cm away from the patient′s neck and at 1 m away from the body were measured by the robot designed for nuclear medicine ward. Kruskal-Wallis rank sum test and Mann-Whitney U test were used to analyze the data. Results: Neck dose equivalent rates for patients with RAT at different time points (4, 24, 48 and 72 h) after ¹³¹I administration were significantly different among 4 groups (H values: 20.889-46.410, all P<0.05), as well as the body dose equivalent rates (H values: 27.181-35.497, all P<0.05). The neck dose equivalent rates at 24, 48 and 72 h after ¹³¹I administration were statistically different between group 3 and 4 for patients with RMLT (z values: 2.328-3.076, all P<0.05; data in group 1 and 2 were too limited to be compared), while there was no statistical difference for the body dose equivalent rates (z values: 0.333-1.621, all P>0.05). The radioactivity retention in patients decreased rapidly within 24 h, then slowed down gradually and became extremely low at 72 h. At 72 h after ¹³¹I administration, 96.6%(255/264) patients with RAT and 100%(33/33) patients with RMLT were lower than 23.3 μSv/h, which meant the patients could be discharged from hospitalization. Conclusions Nuclear medicine ward service robots may dynamically measure residual radioactivity in DTC patients who take ¹³¹I treatment, providing individualized isolation solutions.