OBJECTIVE: To investigate the influences of staphylococcus aureus in planktonic and biofilm forms on the expression of lysozyme, SLPI and gp340 in the human sinonasal explant model. METHOD: Mucosa samples from ethmoid sinus were collected from ten patients of cerebrospinal fluid leak and were cultured with and without S. aureus biofilms and planktonic cells. After the infection, the explant model was confirmed by CLSM, and the secretion of lysozyme, SLPI and gp340 was detected by enzyme-linked immunosorbent assay (ELISA) at 8, 16, and 24 h after S. aureus challenge. Expressions of lysozyme, SLPI and gp340 in mRNA and protein levels after 24 h S. aureus challenge were detected using RT-PCR, immunohistochemistry and Western bolt assay respectively. RESULT: The secretion of lysozyme, SLPI and gp340 in the explant model was observed with a trend to increase in a time-dependent manner. At 8 and 16 h after S. aureus challenge, the secretion of lysozyme, SLPI and gp340 in biofilms group was significantly higher than these in planktonic cells group and control group (P<0. 05). S. aureus biofilms enhanced the mRNA expressions of lysozyme, SLPI and gp340 significantly compared with planktonic cells and controls, and the mRNA expressions in the explant model challenged by planktonic cells were significantly higher than controls (P < 0.05). Although the Western bolt assay showed no differences between the lysozyme expression in the planktonic cells group and control group (P > 0.05), the biofilms enhanced the expressions of lysozyme, SLPI and gp340 significantly compared with planktonic cells and controls (P < 0.05). CONCLUSION S. aureus biofilm induced the expressions of lysozyme, SLPI and gp340 to a higher level than planktonic cells, indicating that S. aureus biofilm was an influencing factor on the innate immune system.
Biofilms ; Enzyme-Linked Immunosorbent Assay ; Ethmoid Sinus ; metabolism ; microbiology ; Humans ; Immunity, Innate ; Muramidase ; metabolism ; RNA, Messenger ; metabolism ; Receptors, Cell Surface ; metabolism ; Secretory Leukocyte Peptidase Inhibitor ; metabolism ; Staphylococcal Infections ; metabolism ; Tissue Culture Techniques

Objective To explore the effects of WeChat-based peer education on improving special position compliance in patient after retinal detachment and to provide reference for clinical nursing staff.Methods Totally 82 patients after retinal detachment who were hospitalized in the First Affiliated Hospital of Zhengzhou University from March 2017 to April 2018 were selected by purposive sampling and randomly divided into the control group (n=41) and the intervention group (n=41) according to the random number table.Patients in the control group received conventional health education,while patients in the intervention group received WeChat-based peer education on the basis of conventional health education.The special position compliance and the incidence rate of complications 3 months post surgery were compared between the two groups.Their satisfaction with the WeChat-based peer education was also evaluated.Results The score of special position compliance in the intervention group was (5.23±0.854),higher than that of the control group (P＜0.01),which was (2.11±0.997).The incidence rate of nuchal muscle pain,headache,dizziness and insomnia caused by special positions in the intervention group was lower than that in the control group (P＜ 0.05).The patients' satisfaction with the WeChat-based peer education was 92.68%.Conclusions The WeChat-based peer education improves patients' awareness of diseases and enhance their confidence in recovery,thereby improving their special position compliance and accelerating recovery.

Objective:To explore the effect of WeChat mini program in immunotherapy management of pediatric patients with combined allergic rhinitis and asthma syndrome (CARAS).Methods:Using the convenient sampling method, 101 children with CARAS who received sublingual immunotherapy in the First Affiliated Hospital of Zhengzhou University from January 2019 to January 2020 were selected as the research objects. The standardized management team of immunotherapy clinic was established. The newly developed specific immunotherapy Wechat applet was used to establish a new model of nurse-patient interaction and communication. The disease knowledge was regularly pushed, medication reminder, nurse telephone intervention and other standardized management were delivered through the Wechat applet to implement continuous nursing for children. The effects of this management model on medication compliance, rhinitis symptoms and asthma control were analyzed.Results:A total of 101 children were included in this study, 3 cases were lost to follow-up, and a total of 98 cases completed the study. After 12 months of intervention, the drug possession score of dust mite drops increased from (83.43±3.68) to (91.78±3.71), the symptom score of allergic rhinitis decreased from (5.83±1.32) to (1.94±0.98), and the score of asthma test questionnaire increased from (18.12±3.99) to (24.97±3.09), the proportion of children with complete control of asthma increased from 48.0% to 90.8%, and the proportion of children without control of asthma decreased from 10.2% to 1.0% (all P<0.05). Conclusion:The immunotherapy management of pediatric patients with combined allergic rhinitis and asthma syndrome based on WeChat mini program can effectively improve the compliance of children and their family, improve the control level of allergic rhinitis and asthma.

Objective:To analyze the risk factors associated with hemorrhagic complications at the puncture site after femoral artery access cerebrovascular interventions and construct a bleeding risk scale.Methods:Two hundred and fifteen patients who underwent femoral artery puncture interventions in the neurointerventional department ward of the First Affiliated Hospital of Zhengzhou University from April to October 2020 were selected for the study, including 60 cases in the complication group and 155 cases in the control group. The patients′ general data, interventional procedure-related data and laboratory tests were analyzed univariately and then incorporated into a binary multi-factor logistic regression analysis to determine independent risk factors, and a bleeding risk scale was constructed according to the regression model.Results:Compared with the control group, there was a statistically significant increase in the incidence of women, diabetes mellitus, use of glucose-lowering drugs, proportion of (anticoagulant/antiplatelet drugs) use and irritability in the complication group ( χ2 values were 4.94-15.81, all P<0.05); age, BMI, HbA1c, prothrombin time activity, and sheath size and operative time were higher than those of the control group, and prothrombin time and creatinine values were lower than those of the control group, with statistically significant differences ( t values of -6.25 to 2.80, respectively, all P<0.05). Multifactorial logistic regression analysis showed that gender ( OR=0.09, 95% CI 0.025-0.302), BMI ( OR=1.736, 95% CI 1.462-2.115), HbA1c ( OR=1.775, 95% CI 1.107-2.845), and use of anticoagulant/antiplatelet drugs ( OR= 0.082, 95% CI 0.015- 0.448) and time to surgery ( OR=1.031, 95% CI 1.012-1.052) were independent risk factors for bleeding complications. In the risk scale: the total score was -3 to 13, and risk existed for a score ≥5. The sensitivity of this scale was 83.3%, the specificity was 92.3%, and the area under the ROC curve was 0.878 ( P<0.001, 95% CI 0.818 to 0.938). Conclusions:Female, high BMI, high HbA1c, anticoagulant/antiplatelet drug use, and long procedure time can increase the risk of bleeding complications at the femoral artery puncture. The predictive model has good predictive performance and can be extended for use.

Objective:To understand the current status and analyze the factors of demoralization of patients with chronic heart failure (CHF), providing references for targeted psychological interventions of nursing staff.Methods:Using the cross-sectional survey method, from August 2022 to January 2023, 282 CHF patients who were followed up in the Cardiovascular Department of the First Affiliated Hospital of Zhengzhou University were selected as the study subjects. They were investigated using the General Information Questionnaire, Demoralization Scale Revised Mandarin Version, Brief Illness Perception Questionnaire (BIPQ), Fear of Progression Questionnaire-Short Form (FoP-Q-SF), and the factors influencing the demoralization of CHF patients were analyzed using univariate analysis and multiple linear regression.Results:Among 282 cases, male 172 cases, female 110 cases, aged (62.29±10.05) years old. The Demoralization Scale Revised Mandarin Version′s score of CHF patients was (30.30 ± 10.37) points; the score of BIPQ was (42.18 ± 13.94) points; the score of FoP-Q-SF was (35.41 ± 7.29)points, which were at high level. The results of multiple linear regression analysis showed that patient disease duration, disease stages, New York heart association cardiac function classification, the score of BIPQ, and the score of FoP-Q-SF were factors influencing the demoralization of CHF patients ( t values were 3.08 to 12.50, all P<0.05). Conclusions:There is an urgent need to focus on the current status of the demoralization of CHF patients. It is necessary to develop a systematic and effective intervention strategy for demoralization, to take into account patient disease duration, disease stages, illness perception, and fear of progression in a comprehensive manner.

Objective To investigate the main allergens in patients with allergic rhinitis in Zhengzhou district.Methods Twenty standardization allergens were used to perform skin prick test in 1 139 patients with allergic rhinitis.The samples were divided into four groups according to the age of the patients.The distributions of different allergens in the four groups were analyzed.Results The top five inhaled allergens were dermatophagoides farina (920 cases,80.8％),dermatophagoides pteronyssinus (870 cases,76.4％),cat hair (517 cases,45.4％),herbs (397 cases,34.9％) and poaceae (353 cases,31.0％) ; the top five ingestive ones were shrimp (143 cases,12.6％),peanut (66 cases,5.8％),egg (56 cases,4.9％),carp (51 cases,4.5％) and meat (48 cases,4.2％).The distribution of allergens was different among the different age groups.Conclusion Dust mites,cat hair,herbs and poaceae were the most common allergens in Zhengzhou district.

Objective To compare the differences between a new type of pillow for nasal drops and traditional pillow in alleviating nasal congestion, nasal pus, sneezing and headache in patients suffering from acute sinusitis, and to select a better nasal dropping equipment for clinical nursing. Methods In total 86 acute sinusitis patients in hospital were randomly divided into the observation group and control group ( for each group, n= 43 ) . Patients in both groups were treated with intravenous cephalosporin, and arcelor thiazole and levofloxacin by nasal dropping. Our new type of pillow for nasal drops and the traditional pillow were applied in the observation group and control group respectively. We compared the differences of treatment effect in five days later between two groups by the SNOT-20 scale, pain assessment, and visual analogue scale. Results The scores of a stuffy nose, discharging, sneezing, headache, decreased smell, taste and unable to sleep, and night sleep of the patients in the observation group were significantly lower than those in the control group ( P <0. 05). The comfort score of the observation group was significantly higher than that of the control group (P<0. 05). Conclusions By using the new pillow for nasal drops, drugs are able to reach the nasal sinus cavity accurately, so the symptoms of patients with acute sinusitis are relieved better.

Objective To construct mobile-health APP intervention program and investigate effects of mobile-health APP on the treatment compliance and quality of life in patients with chronic rhinosinusitis after endoscopic sinus surgery. Methods From March 2016 to May 2017,a total of 83 chronic rhinosinusitis patients hospitalized in the First Affiliated Hospital of Zhengzhou University were randomly divided into observation group and control group, with 42 cases in the observation group and 41 cases in the control group. Patients in the control group were treated with standard discharged nursing, while patients in the observation group received additional mobile-health APP. The Sinonasal Outcome Test-22 (SNOT-22), Treatment Compliance Questionnaire and Visual Analogue Scale (VAS) were used to evaluate patients in two groups before and after the intervention. Results At 3 months after discharge, SNOT-22 and VAS scores of patients in the observation group were lower than the control group; treatment compliance score of patients in the observation group was higher than the control group; there were statistically significant differences between two groups (P<0.01). Conclusions The mobile-health APP can improve treatment compliance, alleviate the nasal symptoms, and increase the quality of life of patients with chronic rhinosinusitis after endoscopic sinus surgery.

Objective To translate Chinese version of the Family Impact of Childhood Bronchial Asthma-Revised(IFABI-R), and to evaluate its reliability and validity in family caregivers for children with asthma. Methods The English version of IFABI-R was translated into Chinese by means of translation, back translation, expert consultation and cultural debugging to form a Chinese version of the questionnaire. A total of 158 parents of the children with asthma treated in the First Affiliated Hospital of Zhengzhou University from January to June 2016 were investigated by the Chinese version IFABI-R. The reliability and validity was tested. Results The Chinese version of IFABI-R has 14 entries. The content validity (I-CVI) of the items ranged from 0.80 to 1.0, and the content validity (S-CVI) of the total scale was 0.98. Exploratory factor analysis extracted 3 factors, the cumulative contribution rate is 68.362%. The Cronbach's alpha coefficient of the scale was 0.843, and the Guttman split half coefficient was 0.842, with the test-retest reliability 0.796. Conclusions The Chinese version of IFABI-R has good cultural applicability, with good reliability and validity. It can be used to assess the quality of life of parents with asthma in children.