1.Association of HLA-A,B and HLA-DRB gene polymorphism with the Susceptibility to HFRS
Mengzhi CHEN ; Qian XU ; Ruiqin DONG
Chinese Journal of Laboratory Medicine 2001;0(05):-
Objective To investigate whether association of the genotype at the HLA A,B and DRB1, B3 B4 B5 loci with the Susceptipility to hemorrhagic fever with renal syndrome (HFRS) in Chinese Hans. Methods HLA A,B antigen types were detected by immunoserologic method and the genotypes of HLA DRB were identified by PCR/SSP method. Results The results showed that The Frequencies of A1, HLA DRB1 * (15,16) and DRB5 were increased in patients as compared to the healthy controls ( P
2.Effects of Psychosocial Intervention on Quality of Life of Students in Key High School
Ruiqin LI ; Li WANG ; Jiwei QIAN
Chinese Mental Health Journal 1991;0(04):-
Objective:To investigate the effects of comprehensive intervention on the quality of life of middle school students. Methods:Eight classes were extracted with striated cluster sampling method from the first grade of two key junior middle schools in city and countryside; four classes (two classes of the urban and rural) were selected randomly as the experimental group and another four classes (two classes of the urban and rural) as the control group. The experimental group received mental-social intervention for three years. No intervention for the control group. The effect was evaluated with the subjective quality of life of children, and a questionnaire on mental health of middle school students. Results:The scores of life quality, cognitive component and emotion component of experimental group were higher than that of control:(172.6?12.8/145.4?17.4,98.7?10.8/85.0?13.3,73.9?4.3/60.4?7.7; t=20.83, 13.20, 25.29 respectively;the average P
3.Effects of kirenol on bovine type II collagen-induced rat lymphocytes in vivo and in vitro.
Yue LU ; Juan XIAO ; Zaiwang WU ; Zheming WANG ; Hongzheng FU ; Yingyu CHEN ; Ruiqin QIAN
Journal of Southern Medical University 2012;32(1):1-6
OBJECTIVETo investigate the effect of kirenol on bovine type II collagen (CII)-specific lymphocytes in vivo and in vitro, and explore the mechanism of kirenol-induced immunosuppression in antigen-specific lymphocytes.
METHODSTwenty-four Wistar rats were randomized into control group, collagen-induced arthritis (CIA) model group, kirenol group (2 mg/kg), and prednisolone group (2 mg/kg). After CII injection, the rats in the latter two groups received intragastric administration of kirenol and prednisolone for 30 days, and the spleens and draining lymph nodes of the rats were harvested to prepare single cell suspensions for measurement of the cytokine levels using ELISA. In the in vitro experiment, the lymphocytes from the control rats, with or without 20 µg/ml CII treatment in the presence of 0-80 µg/ml kirenol, were evaluated for cell proliferation and apoptosis using [(3)H]-thymidine incorporation and flow cytometry, respectively.
RESULTSCompared with those in CIA group, IFN-γ and TNF-α production was significantly reduced in splenocyte culture supernatant of kirenol group (P<0.05 and P<0.01, respectively), and the level of IL-10 and IL-4 was up-regulated (P<0.05 and P<0.01, respectively); IFN-γ and TNF-α secretion by the cultured lymph node cells (LNCs) significantly decreased (P<0.05 and P<0.001, respectively) and IL-10 and IL-4 production increased (P<0.05, P<0.001) in kirenol group. In the in vitro experiment, kirenol treatment caused obvious suppression of CII-induced LNC proliferation and dose-dependently induced antigen-specific apoptosis of the splenocytes and LNCs.
CONCLUSIONKirenol treatment reduces pro-inflammatory cytokine secretion, increases anti-inflammatory cytokine production, inhibits cell proliferation and induces apoptosis of CII-specific lymphocytes in vitro, suggesting the potential of kirenol as an immunosuppressant.
Animals ; Anti-Inflammatory Agents ; pharmacology ; therapeutic use ; Apoptosis ; drug effects ; Arthritis, Rheumatoid ; chemically induced ; drug therapy ; immunology ; Cattle ; Cell Proliferation ; Cells, Cultured ; Collagen Type II ; immunology ; Cytokines ; immunology ; Diterpenes ; pharmacology ; therapeutic use ; Female ; Immunosuppressive Agents ; pharmacology ; therapeutic use ; Lymphocytes ; cytology ; drug effects ; immunology ; Rats ; Rats, Wistar
4. Streptococcal toxic shock syndrome in third trimester: a report of two cases and literature review
Qiuhong YANG ; Min SONG ; Qian SUN ; Xiang WANG ; Aiqing HAN ; Ruiqin SHAN
Chinese Journal of Perinatal Medicine 2019;22(12):872-877
Objective:
To analyze the clinical characteristics of streptococcal toxic shock syndrome (STSS) caused by
5.Preclinical microPET imaging in tumor-bearing nude mice and pharmacokinetic studies of 18F-Alfatide II
Deliang ZHANG ; Yesen LI ; Zuoquan ZHAO ; Jie LU ; Yue WANG ; Qian YU ; Zijing LI ; Pu ZHANG ; Ruiqin CHEN ; Hua WU ; Wei FANG ; Xianzhong ZHANG ; Xiaoyuan CHEN
Chinese Journal of Nuclear Medicine and Molecular Imaging 2019;39(4):201-206
Objective To assess the imaging characteristics of 18F-Alfalide II in different tumorbearing mice and pharmacokinetics in Beagle dogs.Methods BALB/c nude mice(n-24)were used for subcutaneous tumor models(A549 and U87MG),orthotopic lung cancer models(A549)and orthotopic breast cancer models(MDA-MB-231)(n=6 in each group).18F-Alfatide II and 18F-fluorodeoxyglucose(FDG)microPET/CT images were compared in the 4 types of tumor-bearing nude mice models.18F-Alfatide II blocking experiment,biodistribution experiment and imaging studies in tumors of different growth cycles were performed in A549 subcutaneous tumor-bearing nude mice models.Pharmacokinetic experiments were carried out in Beagle dogs(n = 6)and CD-1 mice(n = 9).Two-sample t test was used to analyze the data.Results Compared with 18F-FDG,18F-Alfatide II microPET/CT images showed better imaging quality and contrast in subcutaneous A549,U87MG tumors and orthotopic A549(tumor/heart:4.50±1.17 vs 0.95±0.31;t = 4.125,P<0.01),orthotopic MDA-MB-231(tumor/muscle:6.60±1.53 vs 0.92±0.43;t = 3.984,P<0.01)transplantation nude mice models.18F-Alfatide II could specifically target A549 tumors,and the tumor uptake of 18F-Alfatide II was reduced by about 75% after pre-injection with cyclo(Arg-Gly-Asp-D-Tyr-Lys)(c(RGDyk)).18F-Alfatide II was rapidly cleared from the blood of Beagle dogs(T1/2 was(57.34±11.69)min).It was cleared in the form of prototype drug and(69.24±6.82)% of cumulative dose was excreted through the urine within 4 h after administration.Conclusions 18F-Alfatide II shows a higher target/non-target ratio than,18F-FDG in the imaging of A549,MDA-MB-231 and U87MG tumor-bearing nude mice models,which is more conducive to the diagnosis of tumor.18F-Alfatide II has excellent pharmacokinetic properties.