China is entering into an aging society, and the incidence of hypertension and atherosclerosis were increased year by year. The incidence of transient ischemic attack (TIA) patients with carotid stenosis increased year by year. Carotid artery stenosis or occlusion can cause reduction of local brain tissue perfusion resulting from TIA. CT cerebral perfusion imaging and SPECT could detect earlier the chronic hyper fusion of carotid stenosis TIA, forecast risk coefficient and intervention and prevent the occurrence of cerebral infarction, to evaluate effect of carotid endarterectomy and carotid s carotid artery stenting. It is very important to monitor cerebral hemodynamic changes. The technology has the features of blood infusion situation, advantages, curative effect evaluation and development trend in carotid stenosis TIA reviewed by CT perfusion imaging and SPECT.

Objective:To evaluate the sonodynamic effect of chlorin-e6, a sonosensitizing agent, on the proliferation of MDA-MB-231 cells. Methods:MDA-MB-231 and normal peripheral mononuclear cells (PMNCs) were treated with chlorin-e6 alone or combined with ultrasound. Cell proliferation was determined by MTT assay and cell morphology was observed under inversed fluorescence microscope. Results:Treatment with ultrasound (1.0 MHz, 1.0-2.0 W/cm~2,60 s) or chlorin-e6 (0.10-1.60 mg/mL) alone significantly inhibited the cell proliferation of both MDA-MB-231 and PMNC cells in a intensity-dependent and a dose-dependent manner, respectively. The 50% intensity of ultrasound for inhibiting the growth of MDA-MB-231 and PMNC cells was 1.23 W/cm~2 and 1.25 W/cm~2, respectively (P>0.05) and the IC_(50) of chlorin-e6 was 0.38 mg/mL and 0.77 mg/mL, respectively (P<0.05). Compared with single ultrasound (1.0 MHz,0.5 W/cm~2,60 s) or chlorin-e6 (0.05-0.20 mg/mL) treatment, the combination of ultrasound with chlorin-e6 significantly inhibited the proliferation of MDA-MB-231 cells (P<0.05), while had no synergistic inhibitory effects on the growth of PMNC(P>0.05). Compared with single ultrasound treatment (1.0 MHz, 0.5 W/cm~2,60 s) and single chlorin-e6 treatment (0.20 mg/mL), combination of ultrasound and chlorin-e6 significantly increased the death rate of MDA-MB-231 cells (P<0.05). Conclusion:Ultrasound combined with chlorin-e6 exerted specific inhibitory effect on the proliferation of MDA-MB-231 cells. Chlorin-e6 may be a promising sonosensitizing agent for the treatment of breast cancer.

BACKGROUND AND OBJECTIVESonodynamic therapy (SDT) is a promising new approach for cancer therapy. The purpose of this study is to detect the effects of SDT on the cell proliferation of human lung adenocarcinoma cell SPCA-1, using Chlorin e6 as a sonosensitizing agent activated by ultrasound.

METHODSSPCA-1 and normal peripheral mononuclear cell (PMNC) were treated with ultrasound or Chlorin e6 alone and combined. Cell proliferation was determined by MTT assay, and cell morphology was studied by inverted microscope after 6 h treated.

RESULTS1.0 MHz ultrasound (1.0 W/ cm(2)-2.0 W/cm2 x 60 s) and Chlorin e6 (0.4 mg/mL-3.2 mg/mL) inhibited the cell proliferation of both SPCA-1 and PMNC cells in a intensity- and a dose-dependent manner respectively. Compared with the ultrasound (1.0 W/cm2 x 60 s) or Chlorin e6 (0.05 mg/mL-0.2 mg/mL) alone, the inhibitory effect on the cell proliferation was remarkably increased by the combination of ultrasound and chlorin e6 in SPCA-1 cells (P < 0.05), but no same effect was observed in PMNC cells (P > 0.05). Compared with the ultrasound (1.0 W/cm2 x 60 s) or chlorin e6 (0.2 mg/mL) alone, the combination treatment of ultrasound with Chlorin e6 induced more necrotic cells in SPCA-1 cells (P < 0.05).

CONCLUSIONThere was a significant selectively inhibitory effect of sonodynamic effect with Chlorin e6 on the SPCA-1 cell growth. Chlorin e6 may be a promising sonosensitizing agent for the treatment of non-small cell lung cancer.

Adenocarcinoma ; drug therapy ; therapy ; Cell Line, Tumor ; Humans ; Lung Neoplasms ; drug therapy ; therapy ; Porphyrins ; chemistry ; therapeutic use ; Radiation-Sensitizing Agents ; chemistry ; therapeutic use ; Ultrasonic Therapy ; methods

Objective To study the epidemiological characteristics of family cancer history in patients with nasopharyngeal carcinoma in Dongguan city. Methods A total of 240 patients with nasopharyngeal cancer treated in our hospital from January 2017 to January 2021 were selected for the investigation of family cancer history. The families of the patients were determined respectively, and the family cancer history of the first, second and third relatives was obtained. The incidence, population distribution and incidence factors were investigated and analyzed. Results A total of 7 918 primary, secondary and tertiary relatives of 240 cases were inquired. 188 cases were found to have cancer, including 118 cases of nasopharyngeal cancer and 70 cases of non-nasopharyngeal cancer (10 cases of digestive tract cancer, 18 cases of breast cancer, 20 cases of lymphoma and 22 cases of lung cancer). The incidence of familial cancer was higher in males (127/240) than in females (79/188). The main pathological type of family cancer was squamous cell carcinoma (109/188), accounting for 57.98%. Most of the patients in family cancer group were farmers (128/188), accounting for 68.09%; There was no significant difference in the prevalence of nasopharyngeal carcinoma and non-nasopharyngeal carcinoma in relatives of different genders (χ²=0.11, χ²=0.23, P>0.05). The incidence of cancer in first-degree relatives of different genders was higher than that in second-degree relatives, and the differences were statistically significant (χ²=4.26, χ²=5.62, P<0.05). The peak age of nasopharyngeal carcinoma family cancer patients was 40 to 49 years old group (39.36%). The familial cancer group regular intake of salted fish (>2 times per week), Epstein-Barr virus infection was significantly higher than that of the familial non-cancer group, the difference was statistically significant (χ²=6.57, χ²=10.59, P<0.05). Conclusions The incidence of cancer and nasopharyngeal cancer in first-degree relatives is significantly higher than that in second-level and third-level relatives. The occurrence of nasopharyngeal cancer is the result of the combined action of genetic factors and environmental factors. It is necessary to carry out health education for the families of nasopharyngeal cancer, Avoid eating and curing foods, and actively improve production and living environment, so as to reduce the incidence rate of nasopharyngeal carcinoma.

Objective To analyze the epidemiological characteristics of new elderly malignant tumor cases in Tongren City from 2018 to 2022, so as to provide a theoretical basis for the prevention and control of elderly malignant tumor in this area. Methods A retrospective analysis of the epidemiological characteristics of cases aged 60 and above who were first diagnosed with malignant tumors by pathology in our hospital from 2018 to 2022 was conducted based on the International Classification of Diseases (ICD-10). Results The incidence rate of elderly malignant tumors in Tongren City increased from 123.83/100 000 in 2018 to 126.14/100 000 in 2022, and the incidence rate showed a trend of first rising and then declining. The top five tumors in incidence rate are lung cancer, rectal cancer, liver cancer, stomach cancer and cervical cancer. The tumor order has changed over the years except lung cancer, which was the first. Lung cancer was the main high incidence tumor among the elderly of all ages. With the increase of age, the number of bladder cancer patients increases significantly, and the number of colon cancer patients also shows an upward trend. The prevalence rate of lung cancer（χ²=16.032，P=0.014) , liver cancer（χ²=8.099，P=0.030) , bladder cancer（χ²=11.274 , P=0.018) , and gastric cancer（χ²=19.387 , P=0.011) in elderly people of different sexes was generally higher in men than in women, and the difference was statistically significant (P<0.05). Conclusion Lung cancer , rectal cancer and liver cancer, as the malignant tumors with high case composition and rapid increase in the elderly, can be the focus of early screening and prevention of malignant tumors in the elderly in Tongren City, and men should pay more attention.