Objective To explore the clinical effect of micro-endoscopic resection of thyroid cancer and supply corresponding nursing measures through comparison of micro-endoscopic resection and conventional thyroidectomy.Methods 60 cases of thyroid cancer patients during May 2011 to March 2012 were selected.The experimental group (30 cases) underwent micro-endoscopic resection,while the control group was given conventional thyroidectomy.Items such as the operation time,bleeding volume in operation,complication,incision length,postoperative pain evaluation,postoperative resumption time of activity of neck were compared between the two groups.Results The total operation time was (105.4±28.7)min in the experimental group,and (98.1±22.4) min in the control group respectively.The bleeding volume was (22.4±11.6) ml in the experimental group,and (47.3±18.2) ml in the control group respectively.The postoperative resumption time of activity of neck was (22.3±7.7) h in the experimental group,(39.0±10.8) h in the control group.The above items had significant statistical difference between two groups.The incidence of complication had significant statistical difference between two groups.The incision length of the experimental group was (2.95±0.13) cm,(7.72±0.93) cm in the control group,there was significant statistical difference between two groups.Conclusions Compared with conventional surgery,micro-endoscopic technique in thyroid surgery has good efficacy.And assisting with essential nursing strategy,the effcacy of microendoscopic technique can be improved.

OBJECTIVETo observe the morphological changes of photoreceptor cells in rats with retinitis pigmentosa (RP) induced by N-methyl-N-Nitrosourea (MNU) and the effects of acupuncture against it.

METHODSA total of 16 SD rats were treated with one-time intraperitoneal injection of MNU (50 mg/kg) to induce RP, and randomly divided into an acupuncture group and a model group, 8 rats in each one. In addition, 4 rats were selected as a control group. After model establishment, rats in the acupuncture group were treated with acupuncture at "Xinming-1" (Extra) and "Jingming" (BL 1) for 30 min, once a day for 7 days; rats in the model group and control group received no treatment, and the feeding conditions and fixation were identical as the acupuncture group. 2 h after the end of intervention, rats were sacrificed by cervical dislocation to observe the morphological changes of rhodopsin, rod terminals and rod bipolar cells.

RESULTSDue to the loss of retina photoreceptor cells induced by MNU in rats, in the model group the rhodopsin was stained in residual cell bodies, and there were sporadic rod terminals and little rod bipolar cells; outer segments, inter segments, cell bodies and cell terminals were all affected at different levels. The distribution of rhodopsin was also changed in the acupuncture group, showing more bodies of photoreceptor cells, and the residual rod terminals and rod bipolar cells were more than those in the model group; the injury of retina was less than that in the model group.

CONCLUSIONMNU could lead to a comprehensive injury to the morphology of photoreceptor cells, however, acupuncture is capable of inhibiting morphological changes of photoreceptor cells induced by MNU.

Acupuncture Therapy ; Animals ; Humans ; Male ; Photoreceptor Cells ; cytology ; Rats ; Rats, Sprague-Dawley ; Retinitis Pigmentosa ; physiopathology ; therapy

Objective To investigate the intrapulmonary expression of tumor necrosis factor-? (TNF-?) gene during acute pancreatitis, as well as correlation between TNF-? and lung injury seen in many patients undergoing acute necrotizing pancreatitis (ANP). Methods Ninety SD rats were divided into three groups randomly. Acute edematous pancreatitis(AEP) and ANP were induced by retrograde infusion of sodium taurocholate (0.5% and 5%) into biliopancreatic duct respectively. Animals in the last group only underwent sham-operation. All the animals were sacrificed at certain time, plasma level of amylase and TNF-? was assessed. Intrapulmonary expression of TNF-? mRNA and TNF-? protein was detected by semi-quantitative RT-PCR and immunohisto- chemistry. Cell apoptosis was assessed by TUNEL method. Results Intrapulmonary expression of TNF-? mRNA was much stronger and more sustained in ANP group than that in AEP group (P

Objective To examine the dissimulation in memory test by event-related potentials (ERP).Methods Recording the ERP of 20 students in the choosing tasks according to Binomial Forced-Choice Digit Memory Test (BFMDT) in three situations including reality,dissimulation and forced wrong choose. Results The reaction time was lengthened gradually by the situation of reality ( 681 ± 21 ) ms, dissimulation ( 741 ± 25 ) ms and forced wrong choose( 946 ± 31 ) ms (F= 115.73, P = 0.000). The main components in ERP waveforms, N2 ( 180 ～184 ms) and P2 ( 214 ～ 243 ms) had higher amplitudes in situations of dissimulation and forced wrong choose than that of reality. The amplitude of N2 showed significant difference in three groups(F=6. 896, P＜0.01 ). The amplitudes were increased gradually by the situation with reality( -0.376 ± 0.58 )μV, dissimulation( 0. 86 ± 0.71 )μV and forced wrong choose( 1.77 ±0.82)μV. Conclusion ERP may be an electrophysiological index of the intention of dissimulation in memory test.

Objective To study the level and clinical significance of serum interleukin-18(IL-18)in patients with acute cerebral infarction.Methods The level of serum IL-18 in patient with acute cerebral infarction(n=90)and normal controls group(n=45)were detected by enzyme linked immunosorbent assay.Results The level of serum IL-18 in large,medium and small lesion group were(288.4±59.3);(237.5±58.0);(186.9±50.6)ng/L respeetlvely,which were significantly higher than those in normal control group.The level of serum IL-18 in control group was(147.2±42.8)ng/L(P＜0.05).large lesion group was significantly higher than that in medium and small lesion group(P＜0.05).medium lesion grouP was significantly higher than that in small lesion group(P＜0.05).The levd of serum IL-18 in heavy,medium and light group were(284.9±57.3);(233.4±55.9);(184.5±48.3)ng/L respectively,which were significantly higher than those in normal control group(P＜0.05);heavy group was slgnificantly higher than that in medium and light group(P＜0.05),medium group was significantly higher than that in light group(P＜0.05).Conclusion The level of serum IL-18 was related to the severity of cerebral infarction,IL-18 may be involved in the pathogenesis of the hypoxic ischemic brain injury.

Objective To explore the dynamic change of high mobility group box-1(HMGB1) expression in sepsis shock patients and its relationship with prognosis.Methods A total of 98 patients with septic shock in ICU from March 2014 to March 2016 were collected as the research subjects and divided into the shock group (48 cases) and control group(50 cases) according to whether de-veloping shock at admission.The HMGB1 levels and APACHE Ⅱ scores change within 28 d after admission were compared be-tween the two groups ;the shock group was divided into the group A (HMGB1≤35 pg/mL ,30 cases) ,B(HMGB1>35 pg/mL ,18 cases) according to the HMGB1 level before treatment ,the two groups were observed for 3 months from the day admitted to hospi-tal.The APACHEⅡ score ,ScvO2 ,lactic acid concentration and lactic acid clearance rate after 7 d treatment ,ICU mechanical venti-lation use rate and survival rate within 3 months were compared between the two groups.Results The HMGB1 level and A-PACHEⅡ scores before admission had no statistical difference between the shock group and control group (P>0.05);the HMGB1 and APACHEⅡ scores on 7 ,14 ,21 ,28 d after admission in the shock group were significantly higher than those in the control group ,the difference was statistically significant(P<0.05);the APACHEⅡ score ,mean lactic acid clearance rate and ScvO2 after 7 d treatment in the group A were significantly higher than those in the group B ,the difference was statistically significant (P<0.05) ,the survival rate within 3 months after admission in the group A was also significantly higher than that in the group B ,the difference was statistically significant (P<0.05) ,while the lactic acid concentration and ICU mechanical ventilation use rate after 7 d treatment in the group A were significantly lower than those in the group B with statistical difference (P<0.05).Conclusion The HMGB1 level change has a strong correlation with the prognosis ,the higher the HMGB1 level ,the worse the prognosis of patients with septic shock ,HMGB1 can be used as an important indicator of monitoring disease condition changes in sepsis ,which is worthy of attention in clinical laboratory.

Objective To explore the relation between personality characteristics and event related potential P300 with internet addiction disorder(IAD) of youngsters.Methods Eysenck Personality Questionnaire and event related potential test were used for forty-two IAD and forty non-IAD in youngsters,and the results were compared between the two groups.Results The latency of N2,P3 ((267.2±24.2) ms,(374.5 ±22.0) ms) of event related potential in IAD group were significantly longer and the amplitude of P3 ((3.9± 2.5) μV) was significantly lower than those in non-IAD group((235.7±21.8) ms,(332.3±23.5) ms,(7.8±2.4) μV) (P＜0.01).And the indexes of P,N score (56.2±9.4,49.8±7.4) of Eysenck Personality Questionnaire were significant higher than those in non-IAD group(51.9±9.7,42.4±7.5)(P＜0.01),but the indexes of E,L score (45.7±9.0,44.3±7.9)of Eysenck Personality Questionnaire were significant lower than those in non-IAD group(51.2±9.4,50.9±8.3) (P＜0.01).Correlation analysis showed that there were remarkable positive correlation with latency of P300 and P,N score of Eysenck Personality Questionnaire respectively in IAD group(r=0.49,0.54,P＜0.01),and a significant negative correlation with E,L score(r=-0.43,-0.46,P＜0.01),there was a remarkable negative correlation with amplitude of P300 and P,N score of Eysenck Personality Questionnaire respectively in IAD group(r=-0.41,-0.50,P＜0.01),and a significant positive correlation with E,L score(r=0.45,0.47,P＜0.01).Conclusion IAD in youngsters has personality characteristics and cognitive dysfunction,and it has a relation with personality and event related potential P300.

Objective To explore the changes of cognition and sleep status in the youngsters with internet addiction disorder (IAD), and to clarify the relation between cognition and sleep-related indicators of IAD youngsters.Methods Event related potential (ERP) and Pittsburgh Sleep Quality Index (PSQI)test were used for forty-two IAD (case group)and forty non-IAD (control group) in youngsters, and the indexes of period latency (PL)of N1 ,P2 ,N2 ,P3 and amplitude of P3 (P3 amp)of ERP were analyzed,the factor scores of sleep quality (SQ), sleep latency (SL), sleep time (ST), sleep efficiency (SE), sleep dysfunction (SD), sleeping pills (SP), daytime dysfunction (DD)and total score of PSQI were analyzed. The correlation between the P3 PL, P3 amp of ERP and all the indexes of PSQI in case group was analyzed.Results The PL of N2 ,P3 of ERP in case group was significanly longer and the P3 amp was significantly lower than those in control group (P<0.01);the factor scores of the SQ,SL,ST,SE,SD,DD,and the total score of PSQI test had significant differences between two groups (P<0.01).The correlation analysis results showed that there were remarkable positive correlations between the P3 PL and the factor seores of the SQ,SL,ST,SE,SD,DD and the total score of PSQI test in case group (r=0.46,0.34,0.51,0.40,0.48,and 0.54;P<0.01),and there were remarkable negative correlations betweeb P3 amp and the indexes of PSQI test in case group (r=-0.42,-0.45,-0.49,-0.38,-0.38,and -0.50;P<0.01).Conclusion The IAD youngsters have cognition and sleep dysfunction,and the both factors can affect each other.

Objective To investigate changes of synaptic interface structural in the hippocampus Cal in high and low conditioned place preference(CPP) rats after chronic morphine treatment.Methods The male SD rats were randomly distributed to experiment group 130 cases(intraperitoneal injected morphine twice a day for ten days in an ascending dosage schedule) and control group 30 cases(injected saline of the same volume at same time).The rats in experiment group were re-classified into high preference group(HP),middle group and low preference group(LP) according to the numerical value of the CPP.The middle group was rejected.The rats in HP and LP were scarified at the time of 3h,3d and 14d after the last injection.The hippocampus Cal were removed and prepared for electron microscope specimen.The synaptie interface structure parameter were analyzed by image processing technique.Results ①No significant difference of pretest scores staying at the non-preference chambet existed among the three groups(F=0.78,P=0.47).However,the test scores of the CPP minus the time stayed at pretest natural preference in the high group was significantly higher than that of the low group(P=0.00).②At the 3h and 3d,the PSD of the high group((15.20±-3.65)nm) was significantly lower than low group((17.63±6.61)nm,P<0.01);the synaptic cleft of high group((5.77±2.08)nm) was significantly higher than low group ((4.92±1.65)nm,P<0.05).At the 14d,the PSD of the high group((16.22±4.93)nm) was significantly lower than low group((18.42±3.78)nm,P<0.01).Conclusion In hippocampal Cal area the synaptic cleft in the HP group was higher than that of LP group,the post-synaptic density in the HP group was lower than that of LP group.These changes may be the synaptic basic of the different susceptibility.

Objective To establish the rats model of different susceptibility of heroin addiction,and to explore the possible dopamine D2 receptor (D2R) and dopamine transptor (DAT) mechanism leading to the different susceptibility. Methods 130 male SD rats were carried out CPP training,and the rats were randomly assigned into heroin exposure group (n = 100) and saline control group (SC, n = 30). Heroin exposure group were re-classified into two groups according to the numerical value of the CPP-Pre (the testing score minus that of the pretest):high preference group(HP group) and low preference group(LP group) ,each accounting for 30% of the total rats.The D2R and DAT protein expression of high and low preference group and saline control group rats were detectedwith immunohistochemical method in PFC at 30 minutes and on the 1st,3rd,7th, 14th days after the last injection(149.33 ±2.51 vs 135.83 ±1.78 vs99.33 ±2.84,141.83 ±2.50 vs 131.67 ± 1.87 vs99.17 ±3.61,132.83 ±2.40 vs 122.00 ±2.67 vs 100.33 ±4.26,125.67 ±2.22 vs 113.17 ±2.81 vs 98.33 ±3.25,116.86 ± 1.94 vs 108.63 ± 2.31 vs 98.17 ± 3.82 , respectively, P＜0.05). The D2R protein expressions of HP rats were significantly lower than those of the LP and control group rats (P ＜ 0. 05), and those of LP rat were than lower than those ferent among three groups on addiction phase and 1st,3rd days after the last injection of heroin respectively, respectively (P ＜ 0. 05). The DAT protein expressions of HP and LP rats were significantly lower than those of controlgroup rats (P＜ 0. 05). At all testing time-points, the DAT protein expressions had no significant difference betweenHP and LP group(P＞0. 05). Conclusion D2R and DAT of the rats show appears down-regulation in the PFC after chronic heroin exposure. Different individuals have different D2R sensitivity or receptor levels ,and lower D2R related to the high susceptibility to heroin. Susceptibility to heroin addiction may not be directly related to the expression of DAT.