Objective:To improve the quality standard for Shengji ointment. Methods:A TLC method was used to identify Resina draconis and Olibanum. An HPLC method was used to determine the content of silver sulfadiazine. The detection wavelength was set at 254 nm. The determination was performed on a Wondasil C18 column(250 mm × 4. 6 mm, 5 μm) with the mobile phase consisting of acetonitrile-0. 1% phosphoric acid(8: 92). The injection volume was 5 μl. Results:The TLC spots were clear, specific and repro-ducible. A good linear relationship was obtained between the peak areas and the concentrations of sulfadiazine within the range of 40. 97-1024. 18 μg·ml-1(r=0. 9999). The average recovery was 100. 17% (RSD=0. 64%,n=6). Conclusion: The methods are easy-operated, specific, reproducible and accurate, which can be used for the quality control of the preparation.

Objective To explore the value of asthma journal and corresponding monitoring of pulmonary ventilation function in treating bronchial asthma with the existing classification of asthma severity as control.Methods After 137 patients with bronchial asthma were evaluated according to the existing grades,they kept asthma diaries and received corresponding monitoring of pulmonary ventilation function at least 5 times per day,and all of the above clinical data were analyzed by relevant statistical methods.Results Among the 126 patients who completed monitoring,only 61(48.41%) patients could be graded by the Guide for Prevention and Treatment of Bronchial Asthma;27(21.43%) patients felt sensitive while 33(26.19%) ones felt insensitive.Conclusion The corresponding monitoring can not only accurately evaluate the severity of patients' bronchial asthma condition,but also detect those patients with deviated symptom perception.

Objective:To compare the formula of codeine phosphate tablets produced by two enterprises. Methods: IC50 of the tablets and raw material of codeine phosphate, and the excipients from two enterprises was detected respectively. Results:IC50 of the tablets from A enterprise was 0. 169 mg·ml-1 , that from B enterprise was 0. 140 mg·ml-1 , and the difference was statistically sig-nificant(P<0. 05). IC50 of the raw material from the two enterprises showed no notable difference (P>0. 05). Conclusion: The difference in cytotoxicity of codeine phosphate tablets is manly induced by the excipients, and the products from A enterprise are better than those from B enterprise.

To study the bacteriostat, anti-inflammatory and relieving itching effects of Yinyanjing gel. Methods: A double dilution method was used to detect the in vitro bacteriostat effect of Yinyanjing gel. The therapeutic effects of Yinyanjing gel on bacterial vaginitis were observed in rats. The antipruritic effect of Yinyanjing gel was observed in guinea pigs. Results:MIC for Staphy-lococcus aureus was 47. 39 mg·ml-1 , and that for Escherichia coli was 189. 59 mg·ml-1 . The therapeutic effects of Yinyanjing gel on bacterial vaginitis were significant. Yinyanjing gel showed notable antipruritic effect on the itching induced by histamine phosphate in guinea pigs. Conclusion:Yinyanjing gel exhibits significant effects of bactriostasis,anti-inflammation and relieving itching.

Objective:To study the effect and underlying mechanism of BCG polysaccharide and nucleic acid ( BCG-PSN) in hy-persusceptibility in guinea pigs to explore the improvement method for the quality control model of BCG-PSN. Methods:The ovalbumin induced hypersusceptibility animal model was established, the effect of BCG-PSN on hypersusceptibility in guinea pigs was observed. According to the guideline for immunity toxicity study on Chinese traditional medicine and natural medicine, the hypersusceptibility tests were carried out. Serum IgE and histamine were determined by ELISA. Results:The guinea pigs in the model group and the low dosage BCG-PSN group showed strong anaphylactic symptoms, while the middle and high dosage BCG-PNS groups showed fewer symp-toms. The level of IgE in the model group was (1. 673 0 ± 0. 158 6) μg·ml-1 and (1. 683 1 ± 0. 228 1)μg·ml-1 before and after the attacking, respectively, which was higher than that in the control group(P<0. 01). The levels of IgE in the middle and high dos-age BCG-PNS groups were decreased compared with those in the model group before and after the attacking(P<0. 01). The same re-sults were observed in the levels of histamine. Before and after the attacking, the levels of histamine in the model group was (1. 499 7 ± 0. 133 1) ng·ml-1 and (1. 512 1 ± 0. 050 6) ng·ml-1 , respectively, while the levels of histamine in low, middle and high dos-age BCG-PNS groups were decreased compared with those in the model group before and after the attacking(P<0. 01). Conclusion:BCG-PSN can dose-dependently inhibit the anaphylactic reaction induced by ovalbumin.

Objective To evaluate the agreement between colposcopic diagnosis with 2011 colposcopic terminology of the International Federation for Cervical Pathology and Colposcopy (IFCPC) and cervical pathology in cervical lesions. Methods A retrospective cohort study was performed, which included 376 patients who underwent colposcopy with 2011 international terminology of colposcopy at Obstetrics and Gynecology Hospital of Fudan University from September 2014 to November 2014. With conization or cervical biopsy pathology as the gold standard, the agreement between colposcopic diagnosis and pathologic diagnosis was calculated and correlations between variables were analyzed. Results With 2011 international terminology of colposcopy, agreement of colposcopic diagnosis and cervical pathology was 60.9%(229/376)perfectly matched, and the strength of agreement with weighted Kappa statistic was 0.401 (P<0.01), and agreement within one grade was 97.6%(367/376), which were improved compared with traditional methods. Colposcopic diagnosis were 19.9%(75/376) overestimated and 19.1%(72/376) underestimated. There were no significant difference between agreements in various grade lesions (χ2=1.996, P=0.573). Positive predictive value of high grade colposcopy or more was 84.4%, the negative predictive value of low grade colposcopy or less was 88.8%, whereas false positives were 3.5%and false negatives were 39.3%. A linear trend among three types of transformation zone and patient ages was found (χ2=45.910, P<0.01), whereas lesion sizes were not linearly correlated with lesion degrees (χ2=0.690, P=0.406). In grade 1, grade 2 and nonspecific findings, thin acetowhite epithelium, dense acetowhite epithelium and Lugol′s non-staining were most frequent, the Youden indexes of each were 0.170, 0.373 and 0.145, 0.069 respectively. Positive predictive value of some other findings (such as fine mosaic) and two new signs (inner border sign and ridge sign) were 100.0%. There were no significant difference between agreements in examiners with different levels of experience (χ2=1.197,P=0.550). Conclusions Compared to traditional methods (such as Reid index), 2011 international terminology of colposcopy could improve the agreement between colposcopic diagnosis and pathologic diagnosis, without significant differences by the severity of lesion and the level of examiners′experience. Common findings were classified reasonably, and some signs were highly reliable, which is important for guiding biopsy. However, the reproducibility of transformation zone types and the implication of lesion size need to be further discussed.

Objective:To investigate the expression level of pyruvate kinase M2 (PKM2) in tissues of non-small cell lung cancer (NSCLC) patients and the correlation between PKM2 expression level and radiation sensitivity. Methods:A total of 45 NSCLC pa-tients were chosen and treated with radiotherapy for two months after surgery. The patients were classified into four groups based on the curative effect. The mRNA expression levels of PKM2 in tumor and the homologous paraneoplastic tissues of NSCLC patients were de-tected prior to radiotherapy using real time-polymerase chain reaction (RT-PCR), and the protein expressions of PKM2 in the tumor and paraneoplastic tissues of NSCLC patients were detected with Western blot analysis and immunohistochemical method. The mRNA and protein expression levels of PKM2 in the tumor tissues of different groups were detected with RT-PCR and Western blot assays. Re-sults:The effective rate of radiotherapy for 2 months is 44.8%in NSCLC patients. The expression level of PKM2 is significantly high-er in tumor tissues than in homologous paraneoplastic tissues of NSCLC patients and is negatively correlated with the curative effect of radiotherapy. Conclusion:The expression level of PKM2 is significantly higher in tumor tissues than in the paraneoplastic tissues of NSCLC patients. Patients with lower PKM2 expression level are more sensitive to radiotherapy.

OBJECTIVE:To investigate the utilization of the most used splitting drugs in the inpatients of our hospital,and to promote rational use of splitting drugs. METHODS:The medical orders of oral tablets in the inpatients of our hospital in Jan. 2016 were analyzed statistically in respects of consumption quantity,classification dosage,department dosage and the proportion of scored tablets. RESULTS & CONCLUSIONS:There were 217389 medical orders of tablets in the inpatients of our hospital in Jan. 2016. There were 12792 medical orders of splitting drugs,with utilization rate of 5.88%. The most used splitting tablet were car-diovascular drugs and drug for nervous system,accounting for 53.31% and 20.72% of total. The splitting tablets were mostly used in respiratory and critical care medicine department. Top 10 departments in the list of the number of splitting drug medical orders prescribed 8993 splitting drug medical orders,accounted for 70.30% of total. Top 20 drugs in the list of the number of splitting drugs were involved in 8971 medical orders,accounting for 70.13%of total;there were 14 kinds of scored drugs and 7506 medi-cal orders of splitting scored drugs,accounting for 83.67% of top 20 medical orders of splitting drugs. Among splitting drugs,49 types had scored-line,which were prescribed in 9265 medical orders of splitting drug,accounting for 72.43% of all medical or-ders of splitting drug. Lacking of information in most package inserts about the tablet can or can not split,it is suggested to note this information in package inserts by pharmaceutical enterprises. For commonly used splitting drugs without scored-line,it is pro-posed to produce small-size drugs or add scored-line.

Objective To investigate effects of different ventilation methods during pulmonary surfactant(PS) administration on cerebral oxygen metabolism in preterm infants with neonatal respiratory distress syndrome.Methods Newborns met the inclusion criteria were enrolled into this study,and they were randomly divided into manual group and mechanical group.During PS administration,the proximal end of the tracheal tube was connected to a bag valve mask device in the manual group or a mechanical ventilator in the mechanical group.Brain near infrared spectroscopy monitoring was carried out to detect the cerebral oxygen saturation(ScO2),and the mean arterial blood pressure (MABP) was simultaneously recorded.Results For all 49 preterm infants,PS was administered to preterm infants with severe respiratory distress syndrome treated with mechanical ventilation,including 24 cases of manual ventilation and 25 cases of mechanical ventilation.The left cerebral ScO2 and correlation coefficient of ScO2 and MABP(rScO2-MABP) showed no difference in both groups before PS administration.During administration,ScO2 dramatically increased in both groups [manual group:(85.88 ± 5.54) ％ vs.(77.31 ± 5.40) ％,t =5.521,P =0.000;mechanical group:(83.88 ± 3.18) ％ vs.(76.53 ±4.38)％,t =6.741,P =0.000],and gradually decreased after administration,the level of ScO2 didn't return to the baseline till the 2nd 5 minutes after PS administration [manual group:(79.25 ± 3.02) ％ vs.(77.31 ± 5.40) ％,t =1.560,P =0.220;mechanical group:(78.59 ± 3.45) ％ vs.(76.53 ± 4.38) ％,t =1.832,P =0.074].The same trend of ScO2 change rate was shown simultaneously in both groups.The rScO2-MABP markedly increased during administration in both groups (manual group:2.34 ±0.16 vs.1.86 ±0.21,t =9.022,P =0.000;mechanical group:2.12 ± 0.15 vs.1.87 ±0.21,t =4.810,P =0.000).The rScO2-MABt,in mechanical group rapidly decreased to baseline during the 1st5 minutes (1.84 ± 0.18 vs.1.87 ± 0.21,t =0.538,P =0.635) but went back to baseline in manual group during the 2nd 5 minutes(1.84 ±0.19 vs.1.86-0.21,t =0.350,P =0.809).Change rates of rScO2-MABP were markedly higher in manual group than those in mechanical group during the 1 st 5 minutes (1.15 ± 0.13 vs.1.00 ± 0.15,t =4.943,P =0.000).Conclusions ScO2 could be affected transiently by PS administration with different methods of ventilation.The effect on cerebral autoregulation in mechanical group is shorter than that in manual group.

Objective To observe the changes of abdominal oxygen saturation in very low birth weight infants (VLBWI)with feeding intolerance (FI)within 1 4 days after birth monitored by near infrared spectroscopy (NIRS).Methods VLBWI fitting entry criteria were enrolled into this study.NIRS monitoring was carried out to detect cerebral oxygen saturation (ScO2 )and abdominal oxygen saturation (SsO2 ).Data were analyzed between FI infants and feeding tolerance (FT)infants.FI was defined as follows:gastric residual of more than 50% of the previous feeding volume;emesis or abdominal distention or both;decrease,delay or discontinuation of enteral feedings. Results 93 VLBWI were enrolled.52 cases(55.91 %)presented with FI,including 29 cases(31 .1 9%)of gastric residual increasing and 23 cases(24.73%)of emesis with or without abdominal distention within 1 4 days after birth. The levels of SsO2 and SsO2 /ScO2 showed no differences in infants with FT and with FI within 24h after birth (P >0.05).The change rates of the median of SsO2 and SsO2 /ScO2 in FT infants were similar during 1 4 days (P >0.05).While both the change rates of SsO2 and SsO2 /ScO2 were markedly decreased 1 day before and the day of FI (P <0.01 ).The decreasing degree of SsO2 was similar between infants with gastric residual increasing and infants with emesis with or without abdominal distention[(1 6.2 ±5.1 )vs (1 7.4 ±3.6)%,t =0.733,P =0.476]. Conclusion Abdominal oxygen saturation measured by NIRS may be a useful method for infants adjusting the feeding plan.