Objective To investigate the construction and significance of in vivo pelvis and abdominal-pelvic arterial vascular network in digital three-dimensional (3D) model for uterine artery embolization (UAE) in the application of surgical approach planning based on computerized tomographic angiography(CTA).Methods A series of digital imaging and communications in medicine 3.0 (Dicom 3.0) were obtained from a woman with myoma of uterus by CTA scanning.Then the software Mimics Version 10.01 was used to construct the pelvic and the arterial vascular network 3D model.Results The digital model could clearly display the abdominal aorta,bilateral common iliac arteries,left and right external iliac artery,internal iliac artery and its branches around the stage ; the right uterine artery emitted in the inferior gluteal artery opening below 15.91 mm,the left uterine artery in inferior gluteal artery below the opening of 15.21 mm,the adjacent artery of internal pudendal artery.At the same time,the artery length and angle of bifurcation were accurately measured.The bifurcation angle of abdominal aorta in fifth lumbar vertebral body edge,was 66.58°,the bifurcation angle of right internal iliac artery was 46.23°,the length of right common iliac artery was 51.43 mm,the bifurcation angle between left and external internal iliac artery was 36.45°,the length of left common iliac artery and 67.50 mm.According to the preoperative approach planning,guided wire across the inferior gluteal artery to the lower 15.00 mm and rotating digital subtraction angiography(DSA) tube,could clearly display the uterine artery opening outward by rotating guide wire,which was relatively easy to enter the uterine artery.Conclusion The female pelvic arterial network model in vivo could be successfully constructed by using Mimics Version 10.01 software with database collected through CTA,which may contribute to the materialization of digital models and be used for preoperative surgical simulator.

Objective]To explore the metastasis regulatory of lymph node of papillary thyroid cancer and to analyze the influence factors.[Methods]Clinical data of 375 papillary thyroid cancer patients at our hospital between Jun 2011 and Sep 2015 were retrospectively reviewed and summarized the metastasis regulatory of lymph nodes and the tumor characteristics.[Results]All selected patients were diagnosed papillary thyroid cancer. The Total metastasis rate of cervical lymph node was 67.47%,the metastasis rate of region Ⅵ lymph nodes was 64.27%;the metastasis rate of region Ⅱ~Ⅴ lymph nodes was 36.53%. The metastasis rate of lymph nodes of the patients with tumor diameter over 1 cm,breaking through thyroid membrane and invading the cervical muscle were significantly increased(P < 0.05).[Conclusion]The central group lymph nodes were the most metastasis region of papillary thyroid cancer and should routinely be dissected by the first time of surgery. When the tumor diameter greater than 1 cm or cancer breakthrough thyroid membrane and/or invading the cervical muscles ,the ipsilateral lateral neck lymph nodes should be dissected at the same time.

OBJECTIVETo test the antitumor effect of a human triple-negative breast cancer cell-dendritic cell (DC) fusion vaccine.

METHODSDCs were isolated from fresh peripheral blood of healthy donors. The fusion vaccine was prepared by fusing the DCs and MDA-MB-231 cells via electrofusion. The morphology of the vaccine was identified under inverted fluorescence microscope and the phenotypes were analyzed with flow cytometry. The production of interleukin-12 (IL-12) and interferon-γ (IFN-γ) by the fusion cells was assessed using ELISA. A CCK-8 kit was used to examine the effect of the vaccine in stimulating the proliferation and cytotoxicity of autologous T lymphocytes.

RESULTSThe DCs isolated from peripheral blood mononuclear cells highly expressed CD83, CD86, CD11c and HLA-DR on the cell surface. The fusion cells were irregular in shape and coexpressed the phenotypes of DCs and MDA-MB-231 cells. The fusion cells possessed a strong ability to stimulate the proliferation of T lymphocytes in vitro. Compared with the control group, the fusion vaccine showed a stronger antitumor effect against the breast cancer cells.

CONCLUSIONThe triple-negative breast cancer-DC fusion vaccine prepared by electrofusion can stimulate the proliferation of T lymphocytes and induces strong cytotoxicity of the T cells against breast cancer cells.

Breast Neoplasms ; immunology ; Cancer Vaccines ; immunology ; Cell Fusion ; Cell Line, Tumor ; Dendritic Cells ; immunology ; Female ; Humans ; Interferon-gamma ; immunology ; Interleukin-12 ; immunology ; Lymphocyte Activation ; T-Lymphocytes, Cytotoxic ; immunology