OBJECTIVE:To prepare the vinorelbine-tetrandrine liposomes modified with RGD,and study the inhibitory effect on glioma C6 cells. METHODS:Film dispersion method and ammonium sulfate gradient method were used to prepare the vinorel-bine-tetrandrine liposomes modified with RGD,and the morphology and particle size distribution were observed. The vinorelbine content was determined,and sulforhodamine B method was used to respectively determine the inhibitory effects of blank targeting liposomes,normal vinorelbine liposomes and vinorelbine-tetrandrine liposomes modified with RGD on C6 cells. RESULTS:The prepared vinorelbine-tetrandrine liposomes modified with RGD were spherical or almost spherical with smooth surface,and particle size was about 120 nm. The average content of vinorelbine was 28.27 μg/mL(RSD=0.38％,n=3). Blank targeting liposomes had no significant effect on the growth of C6 cells;vinorelbine-tetrandrine liposomes modified with RGD can obviously inhibit the growth of C6 cells,and cell viability after its effect was significantly lower than normal vinorelbine liposomes (P<0.05). CON-CLUSIONS:Vinorelbine-tetrandrine liposomes modified with RGD are successfully prepared,and they show obvious inhibitory ef-fects on the growth of C6 cells.

Objective To assess the effect of thoracoscopic surgery under laryngeal mask anesthesia and explore the nursing strategies. Methods Thirty-five patients from April to December in 2014 undergoing video-assisted thoracoscopic surgery (VATS) were given intubation anesthesia and another thirty-five ones undergoing the same surgery in 2015 received laryngeal mask anesthesia. The two groups were compared in terms of surgery conditions, surgery complication and ambulation time. Results No significant differences were found in surgery time or blood loss between two groups (P>0.05). The postoperative waking time in the mask anesthesia group was significantly shorter than that of the intubation anesthesia group (P<0.05), and ambulation time was significantly shortened (P<0.05). The rates of throat discomfort, hoarseness and gastrointestinal reactions were significantly lower. Conclusions Laryngeal mask anesthesia used in small thoracoscopic surgery for airway management is safe and feasible. Combined with training in respiration and limb function, better analgesia and nursing, it can keep away complications related to intubation anesthesia, shorten hospital stay and accelerate postoperative rehabilitation.

Objective To explore prognostic factors of the uterine papillary serous carcinoma (UPSC).Methods Twenty eight patients with UPSC in Cancer Hospital of Shantou University Medical College from January 2002 to August 2013 were analyzed retrospectively.Prognostic factors were analyzed by univariate analysis.Results Twenty eight cases of UPSC with 6.3％ occurrence in the endometrial carcinoma admitted in the same period,including 14 cases of staging Ⅰ ～ Ⅱ,and 14 cases of staging Ⅲ.The univariate analysis showed that lymph-node metastasis and operative-pathological staging were significantly associated with the prognosis (P ＜ 0.05),but were not associated with the menopausal,postoperative treatment,operation style,deep myometrial invasion,cervical involvement,and annex invasion (P ＞ 0.05).Two cases of Ia-staging without myometrial infiltration and without added postoperative treatment were still alive.Conclusions Operative-pathological staging and retroperitoneal lymphadenectomy were independently prognostic factors of UPSC.The Ia-staging without myometrial infiltration might be without added postoperative treatment.

Objective To analyse the relationship between the personality and occupation tension of anesthesiologists.Methods One hundred anesthesiologists working at different hospitals in Shenzhen volunteered to complete the questionnaire.Ninety-one valid questionaires were recovered.The questionnaire survey included the Eysenck Personality Questionnaire and Job Content Questionnaire and anesthesia events.The standard scores of internal and external factor,stubborn factor,emotional stability factor,social support,sense of personal achievement and work demand/control ratio (D/C) were calculated.Results Internal and external factor (E) and stubborn factor (P) were negatively correlated with demand/control ratio (D/C).The correlation coefficients were-0.7805and-0.2375.There was no correlation between emotional stability factor (N) and D/C.Social support and sense of personal achievement were negatively correlated with D/C.The correlation coefficients were-0.225 and -0.264.More than 50％ of the study subjects suffered from mental tension during op.eration and fatique after night shift,overtime work and emergency operation.Conclusion Occupation tension is lower in extroverted and higher in introverted anesthesiologists and is also relatively low in those with stubborn personality.Strong social support and deep sense of personal achievement can lower occupation tension.

Objective To know the masons of delaying to receive medical treatment among patients with breast cancer, and then reference to related health education. Methods 13 patients with breast cancer were selected in this research, interviewed them by phenomenological method to know their reasons of delaying to receive medical treatment, analized the results of interview by Clolaizzi method. Results The were five themes for patients with breast cancer about delaying to receive medical treatment, they were not understand breast cancer-related knowledge, shortage of method of self-examination, psychological factors, working pressure and family factors, social environment factors. Conclusions We should strengthen breast cancer-related health education when changed women's traditional ideas and ameliorated their family and social environment.

Objective To investigate the expression and distribution of basic fibroblast growth factor (bFGF)and platelet-derived growth factor (PDGF)in the different phases of femoral neck fracture.Methods Immunohistochemical assays were used to determine the expression and distribution of bFGF and PDGF protein in 36 human specimen of femoral neck fracture.A was measured and analyzed by CMIAS color imaging analysis system for signals of bFGF protein were found high in the mesenchymal cells,monocyte and vascular endothelial cells at 1st week after fracture in 9 subjects,with A of (0.4076 ±0.0902).The weakly positive signals of PDGF protein were found in the mesenchymal cells,while strongly positive in the vascular endothelial cells with A of (0.2261 ±0.0636).At 2rd week,in 9 cases the expression of bFGF and PDGF was strongly expressed in fibroblasts,endothelial cells,cartilage cell and cartilage matrix,osteoblast,with A of[(0.6404±0.0920)and (0.7457±0.0756)]and significandy higher than that at 1st week (P＜0.05,P＜0.01).There was no significant difference between the 3rd and 3nd week with A of[(0.7168±0.1346)and (0.8033±0.0491),P＞0.05 ].The expression of bFGF and PDGF protein was reduced obviously at 4th week but was positive in young and cartilage tissue,with A of [(0.5374correlation between bFGF and PDGF protein in different phases (r1week=0.792,r2week=0.834,r3week=0.880,entiation of cartilage cell and osteoblast,and induce proliferation of vascular endothelial cells and new blood vessel.③ Both bFGF and PDGF are bone growth factors, cooperating in regulating proliferation and differentiation of cartilage cell and osteoblast for fracture healing.

Objective To analyze the characteristics of hospitalized patients with systemic lupus erythematosus (SLE) in Ningxia Hui Autonomous Region,and to characterize the epidemiology of SLE.Methods The data of 184 patients admitted to Ningxia Medical College Hospital during January 2008 to December 2013 were retrospectively analyzed.The data analysis included the initial symptoms,system involvement and clinical situations.Chi-square test,t test,Mann-Whitney U test and Logistic analysis were used for statistical analysis.Results ① The ratio of male and female was 1∶9.8.The most common clinical symptoms was rash in 72 cases (39％),and followed by joint pain in 61 cases (33％),swelling in 28 cases (15％) and fatigue in 21 cases (11％).② Eighteen percent (33/184) patients were late-onset SLE patients.Compared with regular SLE patients,the interval was longer in late-onset SLE patients between clinical symptoms onset and the time when they saw the doctor.③ Blood and kidney were the most commonly affected organs.④ The highest diagnosis rate occurred in rheumatology department,the highest diagnosis rate occurred in the kidney department for patients with dropsy as starting symptom.Conclusion SLE primarily occurs in childbearing aged women.The reason of misdiagnosis of SLE is mainly due to the incorrect hospital selection for the first visit.Delayed diagnosis will lead to delay in the treatment.

Objective To explore the influence of ulinastatin combined with thymic peptide?1on the polarization of T helper(Th) cells,and the immunoregulatory effects on septic patients.Methods Clinical data of 60septic patients admitted to intensive care unit (ICU)of Guangzhou General Hospital of Guangzhou Command from Jun.2006to Oct.2007were collected and randomly divided into treatment group(n=30)and control group(n=30).The control group received classic SSC treatment,while the treatment group was given classical SSC treatment plus ulinastatin and thymic peptide?1.The control group was divided again into death subgroup and survival subgroup according to the living state on day 28.Peripheral blood samples were collected at day 1(before administration)and day 10. CD4+ T cell subset was detected by flow cytometry,and the levels of IFN-?(T helper type-1cytokine)and IL-4(T helper type-2 cytokine)were evaluated semi-quantitatively.Ten healthy volunteers were chosen in the meantime as healthy controls.Results The positive rates of IFN-?and IL-4of CD4+ T cells declined significantly at day 10in treatment group compared with those in control group and before treatment(P0.05).The IFN-?positive rate presented a low level and showed a downward trend after treatment in survival subgroup,while presented a high level and also showed a downward trend after treatment in death subgroup.The IL-4positive rate showed a downward trend in survival subgroup and upward trend in death subgroup.Conclusion Ulinastatin combined with thymic peptide?1may improve the proportion imbalance of IFN-?and IL-4of septic patients,and regulate the immune status.

Objective:To estimate the pharmacokinetics for two solution types of propofol glycoside in-jections in rats .Methods:A high performance liquid chromatography-high resolution mass spectrometry ( HPLC-MS) was established for measuring propofol in rat plasma .Two kinds of propofol glycoside injec-tions were developed and intravenously administered to rats via tail vein , respectively , and a commercial-ly available propofol emulsion injection was intravenously administered as a control .Propofol plasma concentration-time curves were determined , and the pharmacokinetic parameters were estimated .Re-sults:HPLC-MS measurement was performed by using a quadrupole-orbit trap high-resolution mass spec-trometer on a C18 chromatographic column.The mobile phase consisted of water and methanol (20∶80, V/V) .The ion source was an atmospheric pressure chemical ion source , and the negative ion was used for detection with a scanning mode of selective ion monitoring in which m/z 177.127 4 was used for propofol and m/z 149.096 1 used for thymol as an internal standard .A linear correlation between con-centration and peak area ratio was constructed in the range of 50 μg/L-10.0 mg/L propofol.The limit of quantification was 50μg/L propofol .The average recoveries of propofol from plasma were in the range of 93.6% -101.1%, and intra-day or inter-day relative standard deviation for measurement was <14%.The pharmacokinetic results showed that the two kinds of propofol glycoside injections exhibited the same pharmacokinetic behavior .However, the clearance and area under curve values of propofol for the two propofol glycoside injections were evidently increased as compared with those for propofol emulsion injection, respectively.Furthermore, their apparent distribution volumes were increased as well .Never-theless, the propofol elimination half-life (t1/2) value of the newly developed propofol glycoside injections was the same as that of commercial propofol emulsion injection (approximately 1.5 h).Conclusion:The established HPLC-MS method can be used for measuring propofol concentration accurately in rat plasma . The clearance and distribution volumes of propofol glycoside injection are bigger than those of the propofol emulsion injection .