1.Advances in prevention and treatment on inflammation and myocardial ischemia-reperfusion injury by PPAR-γ ligands
Chinese Journal of Pathophysiology 2010;26(1):197-199,208
Peroxisome proliferation-activated receptor-γ (PPAR-γ) is a ligand-activated transcription factor belonging to the nuclear hormone receptor superfamily and participates in the regulation of various metabolic pathways as well as inflammatory responses. PPAR-γ ligands significantly improve myocardial functional recovery and prevent ischemia-reperfusion induced injury. Given the increasing understanding of the cardioprotective effects of PPAR-γ ligands, we know today that the therapeutic effects of PPAR-γ ligands reach far beyond their use as insulin-sensitizers, as many of these agents exert beneficial effects in the conditions associated with ischemia-reperfusion and inflammation.
2.Advances in prevention and treatment on inflammation and myocardial ischemia-reperfusion injury by PPAR-? ligands
Chinese Journal of Pathophysiology 1986;0(01):-
Peroxisome proliferation-activated receptor-? (PPAR-?) is a ligand-activated transcription factor belonging to the nuclear hormone receptor superfamily and participates in the regulation of various metabolic pathways as well as inflammatory responses. PPAR-? ligands significantly improve myocardial functional recovery and prevent ischemia-reperfusion induced injury. Given the increasing understanding of the cardioprotective effects of PPAR-? ligands,we know today that the therapeutic effects of PPAR-? ligands reach far beyond their use as insulin-sensitizers,as many of these agents exert beneficial effects in the conditions associated with ischemia-reperfusion and inflammation.
3.Impact of transtheoretical model on sun protection behaviors of soldiers in plateau area
Weiping LIU ; Hao LU ; Ruijuan HAN
Chinese Journal of Nursing 2017;52(8):986-991
Objective To explore the impact of transtheoretical model on sun protection behaviors of soldiers in plateau area.Methods A total of 208 male soldiers trained in 4 200 meters altitude and medically supported by our hospital were randomly divided into the experimental group(n=104) and the control group(n=104).The experimental group received health education based on the transtheoretical model,and the control group received routine health education.Score of sun protection behaviors questionnaire,sunscreen using behavior and score of the Dermatology Life Quality Index (DLQI) before and after the intervention between two groups were compared.Results Score of sun protection behaviors questionnaire in the experimental group was higher than that in the control group (P<0.05),sunscreen using behavior in the experimental group was better than the control group(P<0.05),score of the Dermatology Life Quality Index in the experimental group was lower than the control group(P<0.05).Conclusion Health education guided by the transtheoretical model can improve the plateau soldiers' sun protection behaviors,and it is an effective method of health education.
4.MCPIP1 induces cell cycle arrest in breast cancer cell line MDA-MB-231
Wenbao LU ; Mingming LIU ; Ruijuan XIU
Basic & Clinical Medicine 2017;37(5):608-613
Objective To investigate the functions of Monocyte chemotactic protein-induced protein 1 (MCPIP1) in human breast cancer cell line MDA-MB-231.Methods MDA-MB-231 cells were transfected with GFP-tagged MCPIP1 by Tet-on inducing expression system.Endogenous MCPIP1 was knocked down by stable expressing shRNA.MTT assay was performed to measure the growth of MDA-MB-231 cells after overexpression or knockdown of MCPIP1.FACS method was used to analyze cell cycle in MDA-MB-231 cells.Real-time PCR was used to test the expression of cell cycle-related mRNAs expression and their half-lives.RNA-IP experiment was conducted to detect the mRNA directly enriched by MCPIP1.Luciferase assay was performed to determine whether the mRNA decay was mediated through 3′UTR.Results MCPIP1 overexpression significantly inhibited cell proliferation(P<0.05), while knockdown MCPIP1 promoted cell proliferation with statistical significances (P<0.05).MCPIP1 induced cell cycle arrest in MDA-MB-231 with statistical significance (P<0.01).MCPIP1 overexpression reduced the half-lives of cell cycle mRNAs (CDK2,CDK6,cyclin D1,cyclin E1,respectively) with significance (P<0.01).In addition, cell cycle-related mRNAs were able to be pulled down by GFP-MCPIP1 but not isotype IgG(P<0.05).Compared with control vector, MCPIP1 significant suppressed luciferase activities of all four 3′UTR reporters (P<0.05).Conclusions MCPIP1 functions as a tumor suppressor in human breast cancer cell line MDA-MB-231 through inducing G1 cell cycle arrest.
5.Application and Contamination of Disposable Syringes:Investigation and Analysis
Ming WEI ; Yueling LI ; Ruijuan HUANG ; Taowen LIU
Chinese Journal of Nosocomiology 2006;0(03):-
OBJECTIVE Passing through the investigation of disposable syringes use condition, to determine amount of syringes used in the hospital and extent of their contact with blood and body fluids, and to assess their waste polluted condition. METHODS By our hospital computer charge system, from May 1, 2006 to Apr 30, 2007 The used syringes were calculated. RESULTS A total of 923 676 plastic disposable syringes were used; from them 6 801 (0.74%) were contaminated by blood, an other body fluids (urine, gastric secretion, cerebrospinal fluid, and wound drainage) or cells, 16 892 (1.83%) were by anticancer drugs and 160 461(17.37%) were with different injections or contrasts. 739 522(80.06%)were utilized for dilute medicine in i.v. drip. CONCLUSIONS Eighty percent of syringes waste is without pollution, it can be used to regard as recycled resources.
6.Perioperative nursing of patients from high altitude earthquake-stricken regions
Mingxia LIU ; Hao LU ; Juan LI ; Ruijuan HAN ; Hua ZHANG
Modern Clinical Nursing 2014;(1):17-18,19
Objective To explore the perioperative nursing of patients from high altitude earthquake-stricken regions. Method The perioperative nursing histories of 18 patients from high altitude earthquake-stricken regions were retrospectively analyzed to summarize the nursing experience.Results No wound infection,crush injury or complications occurred.Sixteen patients had good functional recovery,1 patient was at the stage of recovery about limb movement and another one was also at the recovery period from lumbar vertebral burst fracture complicated with nerve injury.Conclusion Close observation,psychological nursing and functional exercise are important for improved success rate of treatment for the patients from the earthquake-stricken regions.
7.The significance of protein C in acute leukemia patients with disseminated intravascular coagulation
Ruijuan ZHANG ; Xiue LIU ; Linhua YANG ; Bin YU
Journal of Leukemia & Lymphoma 2008;17(2):108-109,113
Objective To investigate diagnostic and prognostic significance of protein C in acute leukemia with disseminated intravaseular coagnlation(DIC). Methods APTT, P-T, D-Dimer, Fbg, antithrombin (AT) and protein C(PC) were determinated in plasma of 44 DIC patients with acute leukemia and 30 normal controls. Results PC was markedly lower in disseminated intravaseular coagulation with acute leukemia group(67.03±36.98) than that of control group (99.53±45.20), and significantly correlation DIC score( r = -0.57,P<0.01). PC of abnormality rate in DIC group was 86.36 %. APTT, PT, D-Dimer, Fbg were significantly increased and AT was decreased during the progress of DIC in relation to values observed in the control group. Conclusion DIC is related to the disorder of coagulation and fibfinolysis system. PC is a sensitive index to diagnose and prognose DIC in acute leukemia.
8.National Natural Science Foundation of China (NSFC) and the progress of basic research of pancreatitis in China
Jian ZHANG ; Weihua GONG ; Xiaowei LIU ; Ruijuan SUN
Chinese Journal of Pancreatology 2014;14(1):3-7
Objective To evaluate the funding and output of pancreatitis related projects supported by National Natural Science Foundation of China (NSFC),and provide suggestions for the funding strategy in the field of pancreatitis research.Methods Database of funded projects and completion reports of NSFC between 1990 and 2012 were retrieved.The number of grants,amount of grants,types,contents,and paper output of funded projects were respectively analyzed.Results From 2000 to 2012,114 projects involving pancreatitis were funded by NSFC,including 71 general programs (62.3%),8 regional programs,34 youth programs (29.8%),1 key program.The total fund during 2011-2012 reached 25.44 million RMB for pancreatitis research,which was 2.76 times higher than that of 2000-2010.According to PubMed search,93 SCI papers funded by NSFC were published during 2011-2012,which was 2.07 times more than that of 2000-2010.The researches mostly focused on molecular mechanism and therapeutic effect of acute pancreatitis,chronic pancreatitis and severe acute pancreatitis,which accounted for 61.4% of all projects.Conclusions The number of grants,amount of grants,and NSFC funded SCI paper output of pancreatitis research have grown steadily,and it indicates that long-term funding of NSFC has played a positive role in promoting the development of clinical and basic research in the field of pancreatitis.
9.Effects of human microRNA-181a-5p on proliferation and migration of gastric cancer cells
Mengna JIU ; Ruijuan XIN ; Xiaogang LIU ; Yaning FENG ; Feihu BAI
Chinese Journal of Digestion 2016;36(6):397-402
Objective To preliminarily explore the effects of human microRNA-181a on migration of gastric cancer cells and its mechanism.Methods The expression of miRNA-181a-5p in gastric cancer cell line GC9811 and peritoneal high metastasis gastric cancer cell line GC9811-P were tested by quantitative real-time polymerase chain reaction (qRT-PCR ). GC9811 cell line was transfected byendogenous synthetic analog mimic and its homologous negative control of miRNA-181a-5p,which were considered as up-regulated group and its control group respectively;GC9811-P were transfected by miRNA inhibitor and its homologous negative control of miRNA-181a-5p,which were considered as down-regulated group and its control group respectively.After miRNA-181a-5p was up or down-regulated,cell proliferation,migration and apoptosis capabilities of gastric cells were detected by matrix thiazolyl tetrazollium (MTT)assay,cloning-forming assay,Transwell migration test,wound healing assays and apoptosis test.After miRNA-181a-5p was up or down regulated,the changes of matrix metalloproteinase (MMP)14 expression were determined by Western blot.Independent sample t test was performed for mean comparison between samples and chi square test was used for rate comparison.Results The results of qRT-PCR showed the relative expression quantity of miRNA-181a-5p in GC9811 was 1 .000 00 ± 0.021 26 and in GC9811-P was 3.175 61 ±0.106 76,and the difference was statistically significant (t =34.620,P <0.01 ).The results of MTT assay indicated that the cell proliferation rate of up-regulated group was higher than that of up-regulated control group,and that of down-regulated group was lower than down-regulated control group.The cloning-forming assay demonstrated that the number of clone forming and clone forming rate of up-regulated group,up-regulated control group,down-regulated group and down-regulated control group were 234.00±10.12 and 46.8%,93.00±9.61 and 18.6%,51 .00 ±7.96 and 10.2%,99.00±8.05 and 19.8%,respectively.The differences between up-regulated group,down-regulated group and their control group were statistically significant (t = 17.500,7.344,χ2 = 12.27, 9.51 ,all P <0.01).The results of Transwell migration experiment showed the number of cells migrated through membrane hole of up-regulated group was 164.00±19.31 ,and which was higher than that of up-regulated control group (87.00±23.04,t=4.436,P <0.05);that of down-regulated group was 157.00± 11 .50,and which was lower than that of down-regulated control group (234.00 ±12.12,t =7.982,P <0.05).The result of wound healing assays indicated that the rate of migration distance of up-regulated group,up-regulated control group,down-regulated group and down-regulated control group were 2.09 ± 0.18,1 .27 ±0.23,1 .15 ±0.15 and 1 .67 ±0.12,respectively.The differences between up-regulated group,down-regulated group and their control group were statistically significant (t =4.863 and 4.689, both P <0.05).The results of apoptosis experiments demonstrated that the apoptosis rate of up-regulated group,up-regulated control group,down-regulated group and down-regulated control group were (6.10± 1 .02 )%,(9.10 ± 2.13 )%,(12.70 ± 1 .23 )%,(8.70 ± 2.54 )%,respectively,and there was no statistically significant difference between up-regulated group,down-regulated group and their control group (both P *0.05).The results of Western blot showed that the grey value of up-regulated group was 561 .881 ±35 .740,which was higher than that of up-regulated control group (275 .784±23.520);that of down-regulated group was 579.565 ±37.950,which was lower than that of down-regulated control group (1 312.760±51 .270),and the differnces were statistically significant (t =11 .580 and 19.910,both P <0.01).Conclusion miRNA-181a-5p highly expresses in peritoneal high metastasis gastric cancer cell line GC9811-P and promoted the proliferation and migration of gastric cancer cell line GC9811 and GC9811-P with a tendency to suppress apoptosis.
10.Application of nurse-in-charge-led ward rounds in multi-level training of nurses
Xiaohong ZHAO ; Jun HOU ; Xiaomei LIU ; Ruijuan ZHANG
Modern Clinical Nursing 2015;(7):58-61
Objective To explore the effect of nurse-in-charge-led ward rounds on the multi-level training of nurses. Methods The traditional nursing ward round was adopted from Juanuary to December 2013 and the nurse-in-charge-led ward round was adapted from January to December 2014. The nurse-in-charge-led round mode and flowchart was developed by designating one nurse-in-charge to carry out the process of nursing round and other nurses in the group to carry out nursing interrogation, check-up, physical examination, nursing diagnosis, making nursing strategy and conclusion. Additionally, the two links of nurse-in-charge-led seminar and nursing feedback were added in the nursing round. The effect of nursing ward round and the results of nursing examination were compared between before and after the improvement. Results A year after implementing new nursing round mode, the effect of nursing round was significantly better than that before the implementation of new mode and the nursing examination score was significantly higher than that before its implementation (all P < 0.05). Conclusion The nurse-in-charge-led round mode can improve the effect of nursing round, improve clinical and professional ability to discover, analyze and solve problems.