Objective To evaluate the effect of valsartan on endothelial function in patients with essential hypertension. Methods Thirty six patients of essential hypertension recieved valsartan(80 mg/d) for 8 weeks. After treatment, serum NO, SOD and MDA were measured using chemically colorimetric method. Brachial artery endothelium-dependent dilation function(FMD) was determined by high resolution ultrasonography. Twenty six health adults served as controls. Results Compared with the control, FMD was significantly decreased (P

Objective　The aim of this study was to investigate the relationship between genetic markers of essential tremor and primary restless legs syndrome/Willis-Ekbom Disease （RLS/WED） in southern Chinese population. Methods　Totally，121 RLS/WED patients and 300 healthy controls were enrolled based on the diagnostic criteria of International RLS Study Group in 2014.MassARRAY and polymer chain reaction （PCR） and sequencing were used to detect 16 single nucleotide polymorphisms （SNPs） and 16 mutations of 15 genes. Results　Our study found that one RLS/WED patient had a heterozygote of p. R1632H of TENM4.However，this patient did not present symptoms of ET. None of other ET risky SNPs was found associated with RLS after Bonferroni correction. Conclusion　Our results suggested p. R1632H mutation of TENM4，may be associated with primary RLS/WED in southern Chinese population.

Objective To study the distribution of the sulfur dioxide (SO2) in the different organs of mice after exposed to SO2. Methods The Kunming mice were exposed to SO2 at different doses in a chamber in which the concentration of SO2 could be monitored. The concentrations of sulfite in livers, kidneys, spleens and testis from male mice were determined by high performance liquid chromatography (HPLC) with fluorescence detection (FD). After reduction, pre-column derivation to tissue homogenates of livers, kidneys, spleens and testis, the mixture was centrifuged, and 5 ?l of the resulting supernatant was directly injected into HPLC with analytical column C18(200 mm ? 5.0 mm, 5 ?m), where the mobile phase consisted of a mixture methanol-water(contained 0.25% acetic acid ) (10:90,V/V), pH was 2.8 adjusted with phosphoric acid, and the exciting and emitting wavelength of fluorescence detection were 392nm and 479nm,respectively. The mobile phase was pumped at a flow rate of 1.0 ml/min, and the column temperature was 30 ℃. Results The standard curve of sulfite was linear in the range from 0.063 ?g/ml to 1.26 ?g/ml (r=0.997 8), and the minimal detectable concentration was 0.05 ?g/ml (S/N=3) with a recovery rates of 95%-101%. The relative standard deviations (RSD) of intra-day and inter-day RSD were less than 10%. The results showed that sulfite concentration in all organs tested from mice in SO2-exposed groups was significantly increased (P

BACKGROUND:Anticancer drug deguelin delays the embryonic development of zebrafish, but its exact mechanism is not yet clear.
OBJECTIVE:To explore expression differences in genes and signaling pathways in deguelin-treated zebrafish embryos.
METHODS:Zebrafish embryos were harvested from zebrafish fed according to the Zebrafish Book. Zebrafish embryos at 2-cel stage were selected and divided into two groups:control group treated with dimethyl sulfoxide incubator liquid, and experimental group treated with 0.6μmol/L deguelin. Total RNA was extracted from the two groups at sphere stage. Then, gene chip technique was used to detect differential y expressed genes in the deguelin-treated zebrafish embryos. Real-time quantitative PCR was employed to validate microarray cluster analysis and pathway analysis to explore the mechanism of action of deguelin.
RESULTS AND CONCLUSION:Chip results showed that 407 genes were upregulated more than three times and 461 genes were downregulated more than three times after deguelin treatment. PCR validation results were consistent with those of the chip. Fourteen pathways were identified by KEGG pathway analysis. Deguelin may play an important role by intervening cel metabolism growth and differentiation.

ObjectiveTo report 18 cases of Bowen dis ea se and erythroplasia of Queyrat.MethodsClinicopatholog ical data of 18 cases of Bowen disease and erythroplasia of Queyrat were present ed.10 cases (mean age 49 year)underwent circumcision and local resection and 8 c ases electric coagulation.Results15 cases have been fo llowed up for 4～48 months.2 cases had new disease at an untreated area and the o ther 13 have been free of the disease.Recurrence ocurred in 2 were cured with 5% 5-fluorouracil cream external application.ConclusionsThe result of the surgical treatment for Bowen disease and erythroplasia of Qu eyrat is good and an alternative therapy with 5-fluorouracil for external appli cation may be considered.Adequate biopsy including deeper tissue is necessary fo r differential diagnosis between carcinoma in situ and invasive penile carcinoma or a variety of lesions of the male genitalia.

Objective To explore the role of apelin in the pathogenesis of diabetic nephropathy.Methods A total of one hundred and fourteen patients with type 2 diabetes mellitus in Qingdao Municipal Hospital from July 2015 to June 2016 were divided into normal albuminuria group (56 cases in the NA group) and micro-albuminuria group (58 cases in the MA group),depending on whether the level of urinary micro-albumin in 24 h was higher than 30 mg,with 60 healthy subjects included as the controls group (NC group).Levels of serum apelin and other biochemical indexes were tested.Results (1) WC,BMI,TC,TG,LDL-C,FBG,FINS,HOMA-IR and apelin in the patients of the NA group and MA group were respectively (87.99±10.27) cm,(24.75±3.35) kg/m2,(5.27±1.28) mmol/L,(1.84±1.25) mmol/L,(3.36±0.91) mmol/L,(7.58±0.87) mmol/L,(13.29±3.57) U/L,4.50±1.41,(364.22±85.03) ng/L ,(90.10±8.97) cm,(25.47±2.82) kg/m2,(5.19±1.11) mmol/L,(2.23±1.43) mmol/L,(3.68±0.83) mmol/L,(7.89±1.11) mmol/L,(15.50±5.00) U/L,5.55±2.27,(397.42±91.29) ng/L,which were higher than those of the NC group ((83.20±5.36) cm,(22.59±2.67) kg/m2,(4.68±1.10) mmol/L,(1.37±0.58) mmol/L,(2.56±0.94) mmol/L,(5.11±0.82) mmol/L,(7.17±2.80) U/L,1.65±0.77,(309.34±68.28) ng/L,P<0.05).FINS,HOMA-IR and apelin in the MA group were significantly higher than those in the NA group (P<0.05).(2) After age,sex and BMI were taken into control,partial correlate analysis showed that serum apelin was positively correlated with LDL-C,FPG,FINS and HOMA-IR (r=0.183,0.314,0.374,0.378,P<0.05).(3) Multifactor regression analysis showed that HOMA-IR and BMI were independent related factors to serum apelin (r2=0.288,0.389,P<0.05).Conclusion Apelin levels in the MA group were significantly higher than those in the NA group,and the serum apelin was positively correlated with HOMA-IR.Thus,apelin may contribute to the pathogenesis and progress of diabetic nephropathy.

Objective:To investigate the characteristic and the hearing change of hearing thresholds of the infants failing in the hearing screening.Method:802 infants(1179 ears) with hearing screening record were reviewed in the study. They had hearing screened by otoacoustic emissions (OAE).However, they failed in the first or & and second hearing screening. Auditory brainstem response (ABR) , distortion product otoacoustic emissions(DPOAE) and acoustic immittance measurement were examined. Subjects with abnormal hearing-evaluation were retested during the following 1-3 months (before 6-month-old). To analysis the characteristic and the hearing change of hearing thresholds of the tests.Result:Among 802 cases(1179 ears),the ratio of single-ear(53.2%) is higher than both-ears(39.5%), P<0.05. And there are no significance between the two groups in the ration of severe abnormal hearing-evaluation ;25.8% infants once suffered from hypercholesterolenia failed in the hearing screening,which was higer than the other known risk factors.P＜0.05; Threatened abortion group has found no cases of severe hearing abnormalities; The ratio of severe abnormal hearing-evaluation in family history hearing loss group (40.9%) was higher than other high-risk factors, followed by two or more risk fators group(10.8%);56.9% of the infance who did not pass the hearing screening were found none risk factors, 42.3% of the infances who had high risk factors was normal hearing level;96 cases(138 ears) undergone twice assesement, in morderat disorder group, more cases(74.3%) changed better , and less disorder group(40.9%), severe disorder group (33.3%).Seven ears became worse; Among abnormal hearing induced by different high-risk factors, the proportion of hearing improvement in children with moderate abnormal hearing was higher than that in children with slight abnormal hearing. Ten cases(12 ears) diagnosised auditory neuropathy.Conclusion:The important factors make the follow-up decision incluing perinatal history,hearing level and age.Maybe some high-factors we did not realized. Gene screening should be paied more attention in the future work.

Objective:To investigate the effects of Chinese Medicinals with right supporting and phlegm transforming actions on albumin synthesis and secretion of fibrous hepatic cells of rat in vitro. Methods: Amygdalin, the water soluble extract of Radix Salviae Miltiorrhizae, lordyceps, and colchicine were used to act on fibrous hepatic cells of rat produced by primary monolayer culture in vitro. The results were determined by ELISA method. Results: Besides colchicine, the others amygdalin and cordyceps. Cordyceps, the water soluble extract of Radix Salviae Miltiorrhizae) showed the obvious raising actions on albumin synthesis and secretion of fibrous hepatic cells of rat ( P

OBJECTIVETo investigate best diagnosing methods and therapy for patients with biliary tract complications after liver transplantation and analyze related factors.

METHODSA review was made of data collected from 96 patients, and confirmed by retrospective case notes examination.

RESULTSA total of 94 patients (97 grafts) survived more than 2 days after transplantation; of whom, 92 had an end-to-end biliary anastomosis with a T tube. The average follow-up was 5.8 months (range: 0.3 - 10.2 months). Among the 94 patients, eight (8.5%, 8/94) had complications: leakage during T-tube removal (2 patients), leakage at an earlier stage (2), simultaneous stricture and leak (2) and just stricture (2). Six patients with biliary tract complications had predisposing factors including hepatic artery stenosis (2 patients, including one hepatic artery stenosis combined with severe rejection, hepatic artery thrombosis (3), and donor-recipient bile duct mismatch (1). There was no difference in cold ischemic time. With hepatic artery thrombosis and/or stenosis > 50%, five patients were re-transplanted; without hepatic artery thrombosis and/or stenosis < 50%, three patients required endoscopic stenting and radiological percutaneous drainage of bile collection with or without balloon dilation. All patients survived.

CONCLUSIONSBiliary strictures occur later than leaks after surgery. Without hepatic artery thrombosis and/or stricture, there is no need for surgery; with hepatic artery thrombosis and/or stricture > 50%, re-transplantation is needed as early as possible.

Adult ; Biliary Tract Diseases ; etiology ; therapy ; Female ; Humans ; Liver Transplantation ; adverse effects ; Male ; Middle Aged ; Postoperative Complications ; etiology ; therapy ; Reoperation ; Retrospective Studies

Acute Pain ; etiology ; prevention & control ; Chronic Pain ; etiology ; prevention & control ; Humans ; Lung Neoplasms ; surgery ; Pneumonectomy ; adverse effects ; methods ; Thoracic Surgery, Video-Assisted