1.Effects of hyperbaric oxygen treatment on glucocorticoid receptor expression in the hipopocampus and on the behavior of rats under chronic restraint stress
Naikeng MAI ; Chen YANG ; Ruijiao SUN ; Yan LU ; Jinjing ZHAO ; Shuyi PAN
Chinese Journal of Physical Medicine and Rehabilitation 2015;37(2):91-94
Objective To explore the effects of hyperbaric oxygen treatment on chronic stress and glucocorticoid receptor (GR) expression in the hippocampus.Methods A total of 60 male Wistar rats were randomly divided into a restraint group,an HBO (hyperbaric oxygen) group,an HBO-restraint group and a control group using a random number table,each group with 15 animals.All the rats in the restraint and HBO groups were constrained by immobilizing their fore-and hind-limbs on a self-made frame for 3h daily for 21 days,and those in the HBO group received HBO treatment once daily for the same 21 days.The HBO-restraint group was immobilized in the morning and treated with HBO in the afternoon.The control group was reared without any special intervention.On the 1st,11th and 21st day of treatment,rats from the different groups were assessed using the open field test.On the 21st day,all the animals were sacrificed and their brains were harvested to detect GR expression.Results In the open field test on the 11 th day,the restraint group scored (131.0 ± 20.6) in terms of motor level and (26.5 ± 4.6) for exploratory behavior,both significantly higher than before restraint and significantly higher than those in the HBO-restraint group at the same time point.Immunofluorescence assay showed that GR expression in the hippocampus of the restraint group was significantly decreased compared with the control group.There was no significant difference,however,between the HBO-restraint group and the control group.Conclusion Chronic restraint stress induces changes in behavior and GR expression in rats which can be alleviated by hypbaric oxygen treatment.
2. Morphologic features of fallopian tubal epithelium in pelvic high-grade serous carcinoma
Ruijiao ZHAO ; Yiying WANG ; Zhen LI ; Kaiyan WU ; Lingfei KONG ; Wenxin ZHENG
Chinese Journal of Pathology 2017;46(8):542-547
Objective:
To study the pathologic features of fallopian tubal epithelium in patients with pelvic high-grade serous carcinoma (HGSC), to investigate its role in pelvic serous carcinogenesis and to reclassify the primary site of HGSC based on recently proposed criteria.
Methods:
The fallopian tubes in 58 cases of pelvic HGSC (54 cases of ovarian primary, 3 cases of tubal primary, 1 case of peritoneum) and 25 cases of pelvic non-HGSC (5 cases of ovarian low-grade serous cancer, 9 cases of endometrioid cancer, and 11 cases of clear cell ovary carcinoma) were collected from June 2015 to December 2016, and serially examined under light microscope (SEE-FIM protocol). Immunostaining for p53 and Ki-67 was performed to evaluate the presence of p53 signature, serous tubal intraepithelial lesion (STIL), serous tubal intraepithelial carcinoma (STIC) and invasive carcinoma in these fallopian tubes. Meanwhile, primary site of HGSC based on the recently proposed diagnostic criteria were also reclassified.
Results:
Among the study group, the frequencies of p53 signature, STIL, STIC and invasive HGSC were 27.6% (16/58), 43.1% (25/58), 36.2% (21/58) and 67.2% (39/58), respectively, while in control group, the proportions were 24.0% (6/25), 0, 0 and 8.0% (2/25), respectively. The continuum of epithelial changes in the process of serous neoplasia including p53 signature, STIL, STIC and invasive carcinoma was identified in 8 cases of pelvic HGSC. The proportions of STIL, STIC and invasive carcinomas in HGSC group were higher than that in non-HGSC group (
3. Chondromucinous tumors involving craniocerebral slope area: a clinicopathological analysis of eight cases
Shang′en FENG ; Bao NIE ; Na LI ; Ruijiao ZHAO
Chinese Journal of Pathology 2019;48(3):199-203
Objective:
To investigate the histological type and clinicopathological characteristics of the craniocerebral slope tumors with chondromucinous features.
Methods:
Retrospective analysis was conducted to analyze chondromucinous tumors in the slope area diagnosed at Henan Provincial People′s Hospital from October 2011 to June 2018. Relevant clinical and pathological data were reviewed, and immunohistochemistry was used to investigate the immunophenotype of the tumors.
Results:
Eight cases were identified, including 4 males and 4 females with patient age ranging from 20 to 48 years. Histologically, there were 1 case of chordoid meningioma, 1 chondromyxoid fibroma, 1 mucinous chondrosarcoma, 1 Maffucci syndrome, and 4 chondroid chordomas.
Conclusion
Chondromucinous tumors of the slope area include chordoma, chordoid meningioma, chondromyxoid fibroma, and myxoid chondrosarcoma and their correct diagnosis is mainly based on the morphological characteristics, immunophenotype and comprehensive analysis of clinical data.