AIM: To investigate whether transferrin rec ep tor (TfR) and Fc ?/? R are the major IgA 1 receptor on human mesangial cells (HMC). METHODS: Serum IgA 1 was isolated by jacalin affinity chromatog raphy and heated to aggregated form (aIgA 1). RT-PCR was performed to investiga te the expression of TfR mRNA and Fc?/?R mRNA. Binding capacity of aIgA 1 to human mesangial cell line (HMCL) was evaluated by radio-ligand binding assay. Bi nding specificity was determined by competitive inhibition assay and phosphoryla tion of extracellular signal-regulated kinase (ERK) was determined by Western bl ot. RESULTS: TfR cDNA and Fc?/?R cDNA products were amplified from HMC but not from HMCL. aIgA 1 was found to bind to HMCL in a dose-dependent, s aturable manner and the binding was inhibited by BSA. Scatchard analysis reveale d a Kd of (6.4?1.7)?10 -7 mol/L and the binding sites were (3.0?1.2) ?106/cells. Both aIgA 1 from patients with IgAN and healthy controls were ab le to induce the phosphorylation of ERK in a similar time-dependent manner, but the effect of aIgA 1 from patients with IgAN was much stronger (P

【Objective】 To explore the relationship between plasma adiponectin levels and hypertension based on the Hanzhong adolescent hypertension cohort. 【Methods】 A total of 361 young individuals with normal renal function， aged 32 to 41 years old， were taken as the research subjects. We collected or measured data including general characteristics， blood pressure （BP）， height， weight， pulse rate， and biochemistry indexes such as fasting glucose， blood lipid and plasma hs-CRP. The concentration of plasma adiponectin was determined by ELISA method. Binary logistic regression was performed to analyze the relationship between plasma adiponectin and hypertension. 【Results】 The plasma adiponectin level in patients with hypertension was significantly lower than that in normotensive patients ［3.56 （2.57-5.02）） μg/mL vs. 4.82 （3.19-6.89） μg/mL， P=0.012］. Partial correlation analysis showed a weak correlation of plasma adiponectin level with systolic BP and diastolic BP （r=-0.155， P=0.003 and r=-0.144， P=0.006）. Binary logistic regression analysis showed that after adjusting for confounding factors such as age， BMI， and high-sensitivity C-reactive protein， the risk of hypertension was 2.46 times higher in patients with plasma adiponectin in the lowest gender-specific tertile than those in the highest tertile （OR=2.46， 95% CI: 1.22-4.99）. 【Conclusion】 Hypoaponectinemia is an independent risk factor for hypertension in young individuals with normal renal function.