1.Blended teaching design and practice based on WeChat platform in oral surgery internship
Rui ZHAO ; Rui HOU ; Yide HE ; Ruichen DAN ; Zi CHAI ; Xiaoyong HUI ; Guangjie XU ; Xia YANG
Chinese Journal of Medical Education Research 2018;17(4):402-405
Based on WeChat platform,blended teaching is designed and implemented in oral surgery practice.The undergraduate intern can get in touch with the internship early,become familiar with it early and make an internship program early.In the process of internship,they can achieve the purpose of getting more questions,more summary and more test through teaching,discussing and contesting.After the internship,they can consolidate,review,exchange and expand learning.The preliminary practice shows that the teaching stimulates the students' interest and motivation in the clinical study of oral surgery,enhances the interaction between teachers and students and the supervision of the teachers to the students,and improves the effect of the practice teaching.
2.Association between body mass index trajectories in children and adolescents of Hanzhong city of Shanxi province and subclinical renal damage in adulthood: a 30-year longitudinal follow-up study
Yang WANG ; Ruichen YAN ; Guilin HU ; Mingfei DU ; Ting ZOU ; Xiaoyu ZHANG ; Chao CHU ; Hao JIA ; Haowei ZHOU ; Chen CHEN ; Yueyuan LIAO ; Qiong MA ; Yu YAN ; Keke WANG ; Yue SUN ; Jiawen HU ; Dan WANG ; Xi ZHANG ; Zejiaxin NIU ; Ke GAO ; Weihua GAO ; Jianjun MU
Chinese Journal of Nephrology 2022;38(3):189-195
Objective:To investigate the association between body mass index (BMI) trajectories in children and adolescents and subclinical renal damage (SRD) in adulthood.Methods:4 623 participants aged 6-18 years old were recruited from the ongoing cohort of Hanzhong adolescent hypertension study in 1987, and the subjects were followed up in 1989, 1992, 1995, 2005, 2013 and 2017, respectively. Group-based trajectory modeling was used to identify distinct BMI trajectories in longitudinal analysis. Generalized linear model was applied to examine the association between different BMI trajectories and SRD incidence in adulthood.Results:A total of 2 678 subjects from childhood to adulthood were enrolled in this study. All subjects were divided into three groups according to three distinct BMI trajectories: low-increasing BMI group ( n=1 017), moderate-increasing BMI group ( n=1 353), and high-increasing BMI group ( n=308). Over follow up for 30 years, a total of 248 participants (9.3%) developed SRD. Urinary albumin-to-creatinine ratio (uACR) in low to high-increasing BMI group was 0.9(0.6, 1.4), 1.0(0.7, 1.7), 1.6(0.8, 3.2), respectively ( P trend<0.001), and estimated glomerular filtration rate was 98.5(87.6, 111.6) , 96.2(86.4, 109.7), 95.3 (87.5, 125.0) ml·min -1·(1.73 m 2) -1, respectively ( P trend=0.025). The generalized linear model analysis showed that uACR was increased linearly from low to high-increasing BMI group [ β=3.16(95% CI 1.02-5.31), Ptrend=0.004]. There was no correlation or linear trend between BMI trajectory and estimated glomerular filtration rate [ β=-2.30(95% CI-5.18-0.57), Ptrend=0.117]. Compared with the low-increasing BMI group, the high-increasing BMI group had greater odds of experiencing SRD in adulthood after adjusting for multiple confounders such as age, gender, medical history and lifestyle ( OR=2.83, 95% CI 1.84-4.36, Ptrend<0.001). Conclusions:Higher BMI trajectorie is correlated with higher level of uACR and risk of SRD in middle age. Identifying long-term BMI trajectorie from early age may assist in predicting individuals′ renal function in later life.
3.Effects of salt intake serum and urinary uromodulin levels in Chinese adults
Yang WANG ; Zejiaxin NIU ; Lan WANG ; Guilin HU ; Ruichen YAN ; Mingfei DU ; Ting ZOU ; Xiaoyu ZHANG ; Chao CHU ; Yueyuan LIAO ; Qiong MA ; Yue YUAN ; Yu YAN ; Yue SUN ; Keke WANG ; Dan WANG
Journal of Xi'an Jiaotong University(Medical Sciences) 2022;43(2):241-246
【Objective】 To explore the effects of dietary salt intake on serum and urinary levels through the chronic salt loading intervention. 【Methods】 Eighty adults (18 to 65 years old) were screened from two villages in Liquan and Lantian counties to participate in a 2-week chronic salt intervention, including a 3-day baseline survey, a 7-day low-salt diet, and a 7-day high-salt diet. Uromodulin levels in serum and urine were determined by enzyme-linked immunosorbent assay (ELISA) kits. According to the baseline blood pressure levels, all subjects were divided into normotensive and hypertensive groups. Pearson or Spearman correlation analyzed the associations of 24 h urinary sodium excretions with serum and urinary levels of uromodulin. 【Results】 At the baseline, serum uromodulin in hypertensive subjects was significantly lower than that in normotensive subjects (26.7±9.9 vs. 57.9±9.7 ng/mL, P=0.033). Serum uromodulin levels were significantly lower on a high-salt diet than on a baseline diet [(54.9±8.8 vs. 28.3±4.5) ng/mL, P=0.007]. In addition, daily urinary excretions of uromodulin were lower on a high-salt diet [(28.4±6.6) ng/mL] than on a baseline diet [(282.1±70.0) ng/mL] and on a low-salt diet [(154.1±21.3) ng/mL]. The 24 h urinary sodium excretions were inversely correlated with urinary uromodulin excretions (r=-0.40, P<0.001) on both low-salt and high-salt diets, but not correlated with serum uromodulin levels. 【Conclusion】 Variations in dietary salt intake significantly affect plasma and urine uromodulin levels.
4.Association of genetic variants in renalase with blood pressure responses to salt and potassium intake
Yang WANG ; Yue SUN ; Guilin HU ; Ting ZOU ; Xiaoyu ZHANG ; Mingfei DU ; Haowei ZHOU ; Hao JIA ; Dan WANG ; Jie ZHANG ; Chen CHEN ; Jiawen HU ; Qiong MA ; Yue YUAN ; Yueyuan LIAO ; Keke WANG ; Yu YAN ; Xi ZHANG ; Zejiaxin NIU ; Yongjuan GUAN ; Ruichen YAN ; Ke GAO ; Min LI ; Jianjun MU
Journal of Xi'an Jiaotong University(Medical Sciences) 2021;42(3):392-397
【Objective】 Based on our previously established salt-sensitive hypertension cohort, we conducted chronic salt loading and potassium supplementation interventions, aiming to examine the association between genetic variants in renalase and blood pressure (BP) responses to dietary interventions of salt and potassium intake. 【Methods】 In 2004, 514 subjects from 126 families were recruited in Shaanxi Province to establish the salt-sensitive hypertension study cohort. Among them, 334 non-parent subjects were selected and sequentially maintained on a low-salt diet for 7 days, then a high-salt diet for 7 days and a high-salt diet with potassium supplementation for another 7 days. Ten single nucleotide polymorphisms (SNPs) in the renalase gene were genotyped on the MassARRAY platform. 【Results】 SNP rs2576178 of the renalasegene was significantly associated with systolic BP (SBP) and mean arterial pressure (MAP) responses to low-salt intervention (SBP: β=-2.730, P<0.05; MAP: β=-1.718, P<0.05). In addition, SNP rs12356177 was significantly associated with diastolic BP response to low-salt diet (β=-1.608, P<0.05). However, we did not find any association for the renalase SNPs with BP response to high-salt diet with potassium supplementation reached nominal statistical significance. 【Conclusion】 Genetic variants in renalase gene are significantly associated with BP response to low-salt diet, suggesting that renalase may be mechanistically involved in BP salt-sensitivity.
5.Associations of genetic variants in M3 receptor with blood pressure responses to salt and potassium intake
Xi ZHANG ; Guilin HU ; Zejiaxin NIU ; Mingfei DU ; Ting ZOU ; Xiaoyu ZHANG ; Lan WANG ; Chao CHU ; Yueyuan LIAO ; Qiong MA ; Dan WANG ; Keke WANG ; Hao JIA ; Chen CHEN ; Yu YAN ; Yue SUN ; Tongshuai GUO ; Jie ZHANG ; Weihua GAO ; Ruichen YAN ; Ke GAO ; Wenjing LUO ; Jianjun MU ; Yang WANG
Journal of Xi'an Jiaotong University(Medical Sciences) 2023;44(1):46-54
【Objective】 M3 muscarinic acetylcholine receptor(M3 receptor), encoded by CHRM3 gene, is widely distributed in the cardiovascular system and plays an important role in cardiac regulation. The aim of this study was to assess the association of genetic variants in M3 receptor with blood pressure(BP) responses to controlled dietary sodium and potassium interventions. 【Methods】 A total of 333 subjects from 124 families were recruited from the rural areas of northern China. After a three-day baseline observation, they were sequentially on a seven-day low-salt diet, a seven-day high-salt diet, and a seven-day high-salt diet plus potassium supplementation. Thirteen CHRM3 single nucleotide polymorphisms(SNPs) were selected for analysis. 【Results】 SNP rs10802811 of the CHRM3 was significantly associated with diastolic BP(DBP) and mean arterial pressure(MAP) responses to both low-salt and high-salt diets while SNPs rs6429147, rs373288072, rs114677844 and rs663148 showed significant associations with systolic BP(SBP) and MAP responses to high-salt diet. In addition, SNP rs6692904 was significantly associated with SBP, DBP and MAP responses to high-salt diet with potassium supplementation. 【Conclusion】 Genetic variants in M3 receptor are significantly associated with BP responses to sodium and potassium intervention, suggesting that M3 receptor may be mechanistically involved in BP salt and potassium sensitivity.