AIM: To study Cordyceps sinesis and its cultured mycelia by HPLC.METHODS: An HPLC established for the fingerprints of cordyceps sinensis and its cultured mycelia was performed on an Agilent C18(4.6 mm ? 25 mm,5 ?m)as column and acetonitrile-0.1% phosphoric acid solution in gradient as mobile phase.The flow rate was 0.5 mL/min,and the detection wavelength was set at 260 nm.RESULTS: There were 6 common peaks in HPLC fingerprint of Cordyceps corensis and its cultured mycelia,three of which were determined as uracil,adenosine,cordycepin.CONCLUSION: The method can be used for the study of fingerprints of cultured mycelia.

Objective To investigate the chemical constituents of Balanophora simaoensis. Methods Chromatography and spectra were used to isolate the constituents and elucidate their structures. Results Four compounds were isolated from whole plant of B. simaoensis and elucidated as methylconiferin (Ⅰ), butylconiferin (Ⅱ), 4-O-?-D-glucopyranosyl confieryl aldehyde (Ⅲ), and brevifolin (Ⅳ), respectively. Conclusion Butylconiferin is a new compound.

Objective To study the chemical constituents of Phlomis medicinalis.Methods The constituents were isolated and purified by various modern chromatography techniques and the structures were elucidated by physicochemical properties and spectral analyses.Results Ten compounds were obtained and elucidated as 1-dehydroxyshanzhigenin methyl ester(Ⅰ),notohamosin A(Ⅱ),verbascoside(Ⅲ),3,4-dihydroxyphenyl ethanol(Ⅳ),succinic acid(Ⅴ),sucrose(Ⅵ),2,6-difructose(Ⅶ),butyl-?-D-fructopyanoside(Ⅷ),D-glucose(Ⅸ),and D-fructose(Ⅹ).Conclusion All the compounds are isolated from this plant for the first time,compound Ⅱ is obtained from the plants of Phlomis Linn.for the first time,and compound Ⅰ is a new one.

Nephrocalcinosis is often accompanied by kidney stone disease. In recent years, more and more nephrocalcinosis has been found to be caused by a monogenic disease, and its pathogenesis has not been fully elucidated. With the development of molecular genetics, more than 30 genes have been found to be the causative genes of nephrocalcinosis. At the same time, with the widespread development of genetic testing technology, more patients have received early diagnosis and timely intervention. This article reviews the clinical and basic research progress of monogenic nephrocalcinosis.

AIM: To affirm marker peaks for the fingerprint chromatography of Shengmai Injection. METHODS: LC-MS/MS method was used, with a Waters symmetryshield TM RP_ 18 column(4.6 mm?250 mm; 5.0 ?m), acetonitrile-water as a mobile phase, The detection wavelength was at 203 nm. Ion trap mass spectrum. RESULTS: Affirming marker peaks for fingerprint chromatography of Shengmai Injection and 10 marker peaks were affirmed. CONCLUSION: The method can affirm marker peaks for the fingerprint chromatography of Shengmai Injection. It is simple, accurate, and has practicality.

Objective: To determine the contents of cinobufagin and resibufogenin in Qianglijiuxin Dripping Pills. Methods: The HPLC method was used at Kromasil C 18 column of 250mm?4.6mm, 5?m, 0.5% Dipotassium Hydrogen Phosphate Acetonitrile (50∶50) as the mobile phase, 1.0mL/min as the flow rate at detection wavelength of 296nm. Results: For cinobufagin, the linear range was 0.67?g～4.7?g and the average recovery rate was 97.78% with RSD=1.2%. For resibufogenin, the linear range was 0.33?g～2.3?g and the average recovery was rate 99.61% with RSD=2.0%.Conclusion: The method can be applied to content determination of cinobafagin and Resibufogenin in Qianglijiuxin Dripping pills.

Objective To study the chemical constituents of Heterosmilax yunnanensis.Methods The compound was isolated and repeatedly purified with chromatography and the structure was elucidated by physico-chemical properties and spectral analysis.Results A new compound was isolated and identified.Conclusion This compound is a novel compound with structure of 1,3,6,7-tetrahydroxy-xanthone-3-O-?-D-glucopyranoside,mamed heterosmioside A.

AIM: To study the application of near infrared spectroscopy for quality control of in-process Wuji Baifeng Pill(Gallus Domesticus,Colla Cornus cervi,Carapax Trionycis,Otheca Mantidis,Radix et Rhizoma Ginseng,etc.). METHODS: Qualitative and quantitative methods for analysis of in-process product was developed by diffuse reflectance near infrared spectroscopy combined with chemometrics.Spectra of 95 samples of Tongren Wuji Baifeng Pill in-process and 19 raw material negative in-process products were collected.Similarity match model was built on the basis of spectra of 75 batches of qualified in-process products.Using this model,the similarity match values of other samples were calculated.Multivariate calibration models for paeonin and water content in in-process product were developed with partial least square algorithm. RESULTS: The results showed that the proposed similarity match model could reflect the quality characteristic of Tongren Wuji Baifeng Pills in-process and reveal the absence and presence of raw materials.The correlation coefficients of the calibration model were within 0.965 7 and 0.992 1 while the values of root mean square error of prediction were 0.005 5% and 0.45%,respectively. CONCLUSION: The experiment shows that the established method is accurate,fast and non-destructive,and can be applied to on-line detection.