1.Extraction and isolation technology of polysaccharide from mulberry leaves
Linhua ZHANG ; Ruichang GAO ; Mingli XU
Chinese Traditional and Herbal Drugs 1994;0(04):-
ZHANG Lin-hua, GAO Rui-chang, XU Ming-li (School of Chemical Engineering, Tianjin University, Tianjin 300072, China)
2.Present Situation of Worldwide Biological Protective Equipment and Chinese Countermeasures
Shutian GAO ; Ruichang WU ; Yundou WANG
Chinese Medical Equipment Journal 1989;0(01):-
Based on the theory of biological protection, this paper introduces the present situation of foreign and Chinese biological protective equipments.Some countermeasures are put forward concerning the development of Chinese biological protective equipment.
3.Present situation of worldwide biosafety laboratory
Shutian GAO ; Ruichang WU ; Yundou WANG
Chinese Medical Equipment Journal 2003;0(11):-
This paper mainly introduces some related problems of biosafety and biosafety laboratory. It also mentions the present situation of worldwide biosafety laboratory and gives out some proposals.
4.Progress in Proteomic Study of the Penicillin Producer---Penicillium Chrysogenum.
Shun WANG ; Peihong WANG ; Nan ZHANG ; Ruichang GAO
Journal of Biomedical Engineering 2015;32(6):1354-1358
Penicillin is a kind of β-lactam drug which has been applied in the clinical treatment firstly in the world, and it has still been widely used at present. The synthesis and regulation mechanism of Penicillium chrysogenum, which is used to produce penicillin, has been studied quite maturely, but its proteomics research started relatively late and fewer reports were published. This paper reviews the synthesis and application of penicillin, transformation of Penicillium chrysogenum, and the research progress of its proteomics. On this basis, the study highlights the advantages of proteomics in the research of protein expression.
Penicillins
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biosynthesis
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Penicillium chrysogenum
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metabolism
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Proteome
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Proteomics
5.Application of HC-videolaryngoscopy in nasotracheal intubation for patients with predicted difficult airway undergoing oral maxillofacial surgery
Xudong YANG ; Ruichang LIU ; Ling GAO ; Zhenzhen LI ; Xiaodong WANG
Journal of Practical Stomatology 2015;(6):833-836
Objective:To investigate the efficacy of HC-videolaryngoscopy in nasotracheal intubation for patients with predicted diffi-cult airway undergoing oral maxillofacial surgery.Methods:70 patients undergoing oral maxillofacial surgery with predicted difficult airway were enroled and randomly divided into 2 groups(n =35).Nasotacheal intubation was performed with HC-videolaryngoscopy (group VL)and Macintosh laryngoscopy(group ML)respectively.The duration and success rate of intubation,degree of glottis expo-sure,hemodynamic response as well as intubation related complications were evaluated.Results:There was higher success rate of first attempt(P <0.05),shorter intubation duration(P <0.05)and fewer frequency of multiple intubation(P <0.05)in group VL than in group ML.During intubation,the first and the best Cormack-Lehane laryngeal view was better in group VL(P <0.05)and less intuba-tion assistance was required in group VL(P <0.05).1 3 patients in group ML were intubated with VL after failure of the first attempt with ML.Conclusion:HC-videolaryngoscopy is safe and effective in the nasotracheal intubation for the patients with predicted difficult airway undergoing oral maxillofacial surgery.
6.Study on Health Service Rescue Vest of Armed Police Forces
Bin MENG ; Hongsheng GAO ; Yundou WANG ; Ruichang WU ; Yin BAI
Chinese Medical Equipment Journal 2003;0(11):-
Objective To evaluate the performance of a kind of military health service rescue vest. Methods The author studied the structure analysis, basic performance experiments and ergonomics tests. Results The vest was scientifically designed, easy to use, in line with the ergonomics and can fulfill the requirements of military health service secure in different environment. Conclusion The secure vest can fulfill medical first aid at scene and military health service rescue. It can be used for soldiers.
7.Progress of Foreign Army's Medical Equipment and Advices to PLA
Xiaofeng ZHANG ; Yundou WANG ; Shutian GAO ; Ruichang WU ; Zaiping SUO
Chinese Medical Equipment Journal 2003;0(12):-
The composition and development of foreign army's medical equipments are discussed,of which,medical equipments for satisfying the requirements of informatized war,NBC protection and MOOTW are paid special attention to.Foreign army also tries hard to enhance medical equipment's capabilities in rapid response and theatre survivability.The advices for development of PLA's medical equipment are also put forward based on the analysis on its present situation.
8.Ultrafiltration and nanofiltration membrane separation technology in extracting and purifying oligosaccharides of Rehmannia glutinosa
Yan DONG ; Ruichang GAO ; Qin PAN ; Xiaonan YAN
Chinese Traditional and Herbal Drugs 1994;0(03):-
Objective Oligosaccharides of Rehmannia glutinosa were separated and purified by different molecular weight cut-off ultrafiltration(UF) membranes,and then ultrafiltrate was concentrated and purified by nanofiltration(NF) membrane.Methods Different molecular weight cut-off UF unit first and then NF unit were used in process.Results Oligosaccharides were separated by twice UF,the optimum separation conditions:the concentration of feed solution was 13—132 mg/mL,the operation pressure was 0.25—0.275 MPa,and the temperature was 20—40 ℃.Then ultrafiltrate was concentrated and purified by NF membrane,the optimum separation conditions:the operation pressure was 0.59—0.79 MPa,the temperature was 20—40 ℃,and the concentration multiplegot to three.Under this condition,the total extraction rate of oligosaccharide products was 46.63% and the purity was above 93.3%,the molecular weights of oligosaccharide products determined by gel filtration were less than 6 000.Conclusion The technology is not only simple and feasible but also easy to separate and purify the oligosaccharides of R.glutinosa effectively.
9.Risk factors for postoperative delirium in patients after vascular free flap reconstruction performed under general anesthesia
Xudong YANG ; Ruichang LIU ; Keying LIU ; Zhinong JI ; Rue FAN ; Ming GUAN ; Fang HAN ; Ling GAO ; Liang JIAO
Chinese Journal of Anesthesiology 2010;30(3):294-296
Objective To investigate the risk factors for postoperative delirium in patients after vascular free flap reconstruction performed under general anesthesia.Methods Two hundred and sixteen ASA Ⅰ-Ⅲ patients aged 18-80 yr undergoing vascular free flap reconstruction surgery were enrolled in this study.Patient characteristics before and during operation were recorded.The patients were followed up for 5 days after operation.Their level of consciousness,severity of pain and sleep quality were evaluated daily.The patients were divided into 2 groups according to the occurrence of delirium during the 5 days after operation:delirium group and non-delirium group.The method of CAM-ICU was reed in the diagnosis of postoperative delirium.Multivariate logistic regression was used to analyze the risk factors for postoperative delirium.Results logistic regression analysis showed that old age,history of alcohol abuse and sleep diacrder after operation were risk factors for delirium developed after free flap surgery.Conclusion Old age,history of alcohol abuse and sleep disorder after operation were the risk factors for postoperative delirium in patients after vascular free flap reconstruction performed under general anesthesia.
10.Survey of Schistosoma japonicum infections in wild animals in hilly transmission-controlled areas of Jiangxi Province
Shang-Biao LÜ ; Nian-Gao CHEN ; Yue-Min LIU ; Li-Yuan ZHOU ; Yi-Song WANG ; Fei HU ; Yi-Feng LI ; Min YUAN ; Dan-Dan LIN
Chinese Journal of Schistosomiasis Control 2019;31(5):463-467
Objective To understand the situation of Schistosoma japonicum infections in wild animals in transmission-controlled schistosomiasis-endemic areas in Jiangxi Province, so as to provide scientific evidence for implementing precision control interventions and achieving the goal of transmission interruption and elimination of schistosomiasis. Methods Five endemic villages from Ruichang City and Pengze County that were heavily endemic for schistosomiasis in Jiangxi Province, were selected as the study villages. Wild animals like wild mice were captured, and the livers of wild animals were purchased from the snail habitats in the study villages for detection of S. japonicum infections. In the study villages, S. japonicum human infections were screened using indirect hemagglutination assay (IHA) followed by parasitological examinations with miracidial hatching test and Kato-Katz method, and the S. japonicum infection in livestock was tested using a miracidial hatching test with a plastic tube. In addition, snail survey was conducted in the study villages by means of systematic sampling combined with environmental sampling, and the S. japonicum infection in snails was detected using a loop-mediated isothermal amplification (LAMP) assay. Results A total of 240 liver specimens were sampled or purchased from 5 species of wild animals in the study villages, including wild mice, weasels, pigs, deer and rabbits. A total of 172 wild mice were captured, with a 2.91% rate of S. japonicum infection, and there was no S. japonicum infection detected in other wild animals. The prevalence of Capillaria hepatica infection was 12.21%, 1.96% and 12.50% in wild mice, deer and pigs, respectively. In addition, there was no S. japonicum infection found in either humans or livestock in the study villages, and the mean snail density varied from 0.13 to 0.80 snails/0.1 m2 in the study villages. LAMP assay detected S. japonicum infection in 2 tubes in a study village. Conclusions The role of wild animals in schistosomiasis transmission and their potential risks can not be neglected in hilly schistosomiasis-endemic areas of Jiangsu Province after transmission control. Intensified surveillance and targeted control measures should be implemented to consolidate schistosomiasis control achievements.