From 38 pieces of deep\|sea samples,a strain,DY\|A,which produces protease was isolated.It can grow at a wide range of pH and salt concentration and best at 10℃.The strain produced maximum protease activity after growth at 10℃,initial pH 10 0 and inoculation volume 0 5%for 48～72h on a shaker by speed of 200r/min.The optimum pH and temperature for the protease activity production are pH 10.0 and 40℃.The enzyme is alkaline protease.Some other properties of the strain and the protease were discussed in detail.

Objective To explore the roles of IκBα and TGF-β1 on airway inflammation in rats with chronic bronchitis induced by smoking and study the effects and mechanism of anti-inflammation of pretreatment with ambroxol. Methods Sixty male wistar rats were randomly divided into four groups: Normal dosage group, model group, high dosage group and low dosage group. The rats with chronic bronchitis were established by smoking. For high and low dosage group, rats were pretreated respectively with ambroxol group, rats were pretreated with normal saline through peritoneal injection as much as the low dosage group.After 76 days, the histopathologic changes stained in hemotoxylin and eosin (H. E.) of bronchopulmonary tissues were observed under opticalmicroscope, white cell counts and differential analysis were performed in BALF, the expression of IκBα and TGF-β1 were detected by immunohistochemistry. Results The pathological changes of model group were in consistent with that of chronic bronchitis, but the degrees were significantly reduced in high and low dosage groups. Compared with those in normal group, the white cell count and the neutrophilic granulocyte count of BALF in model group were significantly increased and the macrophagocyte count decreased, and the expression of IκBαwas significantly decreased(t =3.24,3.31,3.29,3.48, P ＜0. 05) and the TGF-β1 significantly increased (P ＜0. 05). Compared with those in the model group, the white cell count and the neutrophilic granulocyte count of BALF in high and low dosage group were significantly decreased and the macrophagocyte count increased, the expression of IκBαwas significantly increased (t =2. 86,2. 97,2. 92,3.52,2.42,2. 88,2. 58,3.48, P ＜0. 05) and the TGF-β1 significantly decreased (P ＜0. 05) . Compared with those in low dosage group, the expression of TGF-β1 was decreased and the expression of IκBαincreased in high dosage group (t =2. 82,3.64, P ＜0. 05). Conclusions Down-regulating the expression of IκBα and up-regulating of TGF-β1 may be involved in the process of airway inflammation in rats with chronic bronchitis induced by smoking. Ambroxol might have better effects on ameliorating airway inflammation by up-regulating the expression of IκBα and down-regulating of TGF-β1.

Objective To investigate the origin of prostatic blood-supply by digital subtraction angiography (DSA) and provide references for the arterial embolization of prostatic hyperplasia. Methods A total of 72 male patients with successful selective and super-selective prostatic arterial catheterization angiographies were observed and recorded the original arterial blood-supply of the prostate, number of the prostatic arteries and the predominant blood supply. Results The blood-supply of prostate is very complicated including the original prostatic arterial supply from the inferior vesicle arteries (69); the internal iliac arteries (63); the internal pudendal arteries (52); the inferior rectum arteries (29); the superior vesicle arteries (14). The predominant arterial supply include the internal iliac arteries (37); the inferior vesicle arteries (20); the internal pudendal arteries (6) and the inferior rectum arteries (2). Conclusion Arterial contrast medium angiography can correctly demonstrate and assess the original arterial supply of prostate, providing significant guidance for further interventional embolization.

Cardiomyopathies ; congenital ; Heart Ventricles ; pathology ; Humans ; Infant, Newborn ; Male ; Myocardium ; pathology

Adult ; Aluminum Silicates ; toxicity ; Humans ; Middle Aged ; Mining ; Occupational Exposure ; Prevalence ; Silicosis ; epidemiology

This study reported the analysis of plasma phospholipid metabolism of the rats and the pathological biomarkers between the type 2 diabetes model control group (MC) and the normal control group (NC). SD rats were randomly divided into 2 groups: NC and MC. To investigate state of plasma metabolite profiling in normal body, type 2 diabetes mellitus (T2DM) model group using UPLC-Q-TOF/MS which was used as analysis tool in this research. The compounds were identified by UPLC-Q-TOF/MS based on MS/MS fragment ions information, element composition in MassLynx 4.1 and the Lipid Maps database. The sign of two groups of samples in specific markers for screening was through a software package in R software (BioMark software). The results show that the pathological markers were mainly phosphatidylcholine (PC) and triglycerides (TG); the 2-acyl PC in the MC group was less more obviously than that in the NC group; high carbon number and high degree of unsaturation of the TG was reduced under the condition of type 2 diabetes. In the state of type 2 diabetes, metabolic changes occurred in rat plasma phospholipids obviously, which had a close relationship with the occurrence and development of T2DM.

Objective To investigate the regular,variant and parasitic arterial blood supply in liver cancer,so as to improve the effects of treatment on liver cancer by TACE.Methods 52 liver cancer focus confirmed by pathology and imaging were retrospectively analysed First,the routine DSA was performed,if following conditions including no tumor staining or no vessel area within tumor on arterial phase and filling defect within tumor on parenchyma phase were presented,then DSA via superior mesenteric artery,left gastric artery,inferior phrenic artery,right adrenal artery,internal thoracic artery and intercostal artery were perfomed again.Results 39 focus with regular arterial blood supply(75%),13 focus with variant (25%) and 11 focus with parasitic arterial blood supply (21%) are observed .Conclusion The liver cancer focuses are mainly fed by the regular arterial blood supply of liver artery ,but still 25% are by variant arterial blood supply.The chemoembolization for liver cancer through regular ,variant and parasitic arterial blood supply can improve the therapeutic effect.

Objective To evaluate the efficacy of arterial embolization in the therapy of benign prostatic hyperplasia(BHP).Methods After super-selective arterial contrast medium administration and comfirmation of prostate blood-supply artery,polyvinyl alcohol(PVA)and gelfoam were injected via the arterial catheter in 12 patients diagnosed as BHP.Pre-and post-operative I-PSS,quality of life(QOL),maximum urine flow rate(Qmax),and residual urine(RU)were compared.At the same time,the changes of prostate volume and blood supply were evaluated by endorectal ultrasound and CT.The change of urethral diameter was also evaluated by urethral contrast medium.Results A total of 21 prostate blood-supply arteries were embolized,including 5 internal iliac arteries,9 inferior vesical arteries,5 internal pudendal arteries,and 2 obturator arteries.Bilateral arterial embolization was performed in 9 patients and unilateral embolization was performed in 3 patients.The post-operative I-PSS(4.8),QOL(1.3),Qmax(18.9 ml/s),and RU(3 ml)were obviously improved compared with those of pre-operation(24.2,4.8,9.6 ml/s,and 138 ml).The prostate blood supply was obviously decreased and the maximum speed of blood stream was slackened from(19.3?9.8)cm/s to(10.0?8.9)cm/s.The prostate volume decreased from pre-operative 127.6 ml to post-operative 65.1 ml.The reduction rate was 51%.Conclusion The arterial embolization in the therapy of BHP was a new method.It had some advantages,such as less injury,higher efficacy,better safety and fewer complications.

To investigate the etiology and differential diagnoses of patients with systemic lupus erythematosus (SLE) and fever of unknown origin (FUO).From January 2012 to December 2014,a total of 928 SLE patients were admitted to Peking Union Medical College Hospital.Only 50 patients were combined with FUO (5.4％).The most common reason of fever was caused by infections (33 cases,66.0％),including bacterial infection in 17 cases with 5 tuberculosis,viral infection in 11 cases,and fungal infection in 5 cases.The second reason offever was due to poor disease control or recurrence in 17 patients (34.0％).No fever was caused by malignant tumor.When clinical data was compared between 17 non-infected patients versus 33 infected patients,C reactive protein and procalcitonin in the infected group were significantly higher than those in the non-infected group.In SLE patients combined with FUO,infection is the most common etiology which is necessary to be paid attention to.

BACKGROUND:Chroming can improve the corrosion resistance of soft magnetic aloys. OBJECTIVE:To identify the optimal chroming conditions of two soft magnetic aloys, Fe-Cr-Mo and Fe-Ni, so as to improve their corrosion resistance. METHODS:Twelve pieces of square-shaped Fe-Cr-Mo and Fe-Ni soft magnetic aloys were casted in the size of 20 mm×20 mm×2 mm. After sanding and polishing, chrome plating was performed on these specimens and the coating thickness and hardness were measured. The selection factors included electroplate type (hexavalent chromium and trivalent chromium), current density (15, 20, 25, 35, 40, 45 A/dm2) and electroplating time (30, 40, 60 minutes). The optimal chroming conditions were determined by one-factor experimental method and the chrome plating was observed under a scanning electron microscope. RESULTS AND CONCLUSION:Cr6+ coating was significantly better than Cr3+ coating in the aspects of hardness, thickness and uniformity, indicating Cr6+ coating can significantly improve the corrosion resistance of Fe-Cr-Mo and Fe-Ni aloys. For Fe-Cr-Mo soft magnetic aloy, the optimal conditions of Cr6+ coating were as folows: current density of 45 A/dm2 and electroplating time of 60 minutes; the optimal conditions of Cr3+ coating were as folows: current density of 20 A/dm2 and electroplating time of 30 minutes. For Fe-Ni soft magnetic aloys, the optimal conditions of Cr6+ coating were as folows: current density of 35 A/dm2 and electroplating time of 60 minutes; the optimal conditions of Cr3+ coating were as folows: current density of 20 A/dm2 and electroplating time of 40 minutes.