OBJECTIVETo observe the efficacy of combination therapy of clomiphene and Chinese drugs for nourishing Shen and activating blood circulation (NSABC) in treating Stein-Leventhal syndrome caused sterility.

METHODSSixty-two patients with anovulation caused sterility were randomly divided into the treated group (n = 32) and the control group (n = 30). The treated grop was treated with the combination therapy and the control group treated by the same dosage of clomiphene alone.

RESULTSAfter treatment, when comparing with that before treatment, the endocrine hormones in the treated group improved significantly, showing a markedly decrease of androgen and luteotropic hormone, and increase of estrogen (P < 0.001). The periodic ovulation rate in the treated group reached 87%, the total pregnancy rate being 65.6%, with no occurrence of ovarian hyperstimulation syndrome (OHSS) and luteinized unruptured follicle syndrome (LUFS), while in the control group, the periodic ovulation rate was 66%, the total pregnancy rate 36.6%, with LUFS occurred in 4 patients. Comparison of the therapeutic effects between the two groups showed significant difference (P < 0.05).

CONCLUSIONThe combination therapy of clomiphene and NSABC has a better therapeutic effect in treating Stein-Leventhal syndrome caused sterility than that of using clomiphene alone.

Adult ; Androgens ; blood ; Anovulation ; blood ; etiology ; Clomiphene ; therapeutic use ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Infertility, Female ; blood ; drug therapy ; etiology ; Ovarian Hyperstimulation Syndrome ; prevention & control ; Ovulation Induction ; Phytotherapy ; Polycystic Ovary Syndrome ; blood ; complications ; drug therapy ; Progestins ; blood

Objective To investigate the numbers and the expression of MHC-Ⅰpositive cells in hu- man ovarian epithelial carcinoma tissues and provide the experimental data in the futher biological therapy of ovarian carcinoma.Methods Thirty one samples of ovarian epithelial carcinoma were analysed with flow cy- tometry for MHC classⅠexpression.Results The number of MHC classⅠcells(25.22?21.23,4.37?3.63)was fewer in ovarian epithelial carcinoma than that in normal ovarian tissues(43.56?18.47,7.43?5.87),and was related with pathologic grades(P

Animals ; Bone and Bones ; chemistry ; Female ; Ovariectomy ; adverse effects ; Ovary ; surgery ; Rats ; Rats, Sprague-Dawley ; Receptors, Estrogen ; analysis ; Transforming Growth Factors ; analysis

OBJECTIVETo observe the clinical effect of Jingling oral liquid (JLOL) in treating infertile patients with varicocele after varicocelectomy.

METHODSSixty patients were randomly divided into two groups, the 30 patients in the treated group treated by JLOL, and the 30 in the control group treated with intramuscular injection of human chorionic gonadotropine (hCG).

RESULTSThe pregnant rate of patient's wife in the treated group was 76.6%, while that in the control group was 40.0%, showing significant difference between them (P < 0.05). The quality of semen was improved in both groups (P < 0.05 or P < 0.01), and the improvement was better in the treated group than that in the control group (P < 0.05). The reproductive hormones were also improved in both groups (P < 0.01). In the treated group, levels of superoxide dismutase (SOD) and zinc in semen increased, and that of cadmium decreased after treatment, as compared with those before treatment, the difference was significant (P < 0.05).

CONCLUSIONJLOL could improve and regulate the reproductive hormone disturbance in infertile patients with varicocele after varicocelectomy, enhance their quality of semen and sperm to increase the pregnancy rate of their spouses.

Drugs, Chinese Herbal ; therapeutic use ; Humans ; Infertility, Male ; drug therapy ; etiology ; Male ; Phytotherapy ; Postoperative Care ; Semen ; chemistry ; Sperm Motility ; Superoxide Dismutase ; metabolism ; Varicocele ; complications ; drug therapy ; surgery ; Zinc ; metabolism

OBJECTIVETo explore the impact of microwave radiation on GC-2spd cells.

METHODSWe exposed cultured GC-2spd cells to microwave radiation at the average power densities of 0, 10 and 30 mW/cm2 for 15 minutes and, from I to 24 hours after the exposure, we observed the changes in cell proliferation, histology and ultrastructure, cell apoptosis, and cAMP content by MTIT, light microscopy, electron microscopy, flow cytometry and ELISA.

RESULTSCompared with the control group, the GC-2spd cells showed a significant decrease in proliferation ability at 1 -24 hours after 10 and 30 mW/cm2 microwave radiation, except at 12 hours after 30 mW/cm2 radiation (P <0.05 or P <0.01), with reduced length and number of cell enation and increased intra cytoplasm vacuoles. The rate of cell apoptosis (%) was significantly increased in the 10 and 30 mW/cm2 groups at 6 hours (4.56 +/- 2.09 vs 14.59 +/- 1.09 and 8.48 +/- 1.73, P <0.05 or P <0.01) , with agglutination and margin translocation of chromatins and obvious dilation of endo cytoplasmic reticula. The cAMP content (nmol/g) in the GC-2spd cells was remarkably reduced in the 10 and 30 mW/cm2 groups at 6 and 24 hours (2.77 +/-0.24 vs 1.65+/- 0. 17 and 1.96+/-0.10, 3.02 +/-0.47 vs 2.13 +/-0.33 and 1.69 +/-0.27, P <0.05 or P <0.01).

CONCLUSIONMicrowave radiation at 10 and 30 mW/cm2 may cause injury to GC-2spd cells, which is manifested by decreased content of intracellular cAMP, reduced activity of cell proliferation, and increased rate of cell apoptosis.

Animals ; Apoptosis ; radiation effects ; Cell Line ; radiation effects ; Cell Proliferation ; radiation effects ; Male ; Mice ; Microwaves ; adverse effects ; Spermatocytes ; radiation effects

OBJECTIVETo determine the molecular basis of late onset ornithine transcarbamylase (OTC) deficiency in a Chinese family of Han nationality and the exon sequences of OTC gene of this patient.

METHODSPolymerase chain reaction-single strand conformation polymorphism and direct sequencing were used to identify the mutation type.

RESULTSA missense mutation E122G in the conserved residue of exon 4 was identified which is unreported before.

CONCLUSIONThe E122G mutation in human OTC gene may cause late onset OTC deficiency.

Age of Onset ; Base Sequence ; Child, Preschool ; DNA ; chemistry ; genetics ; DNA Mutational Analysis ; Family Health ; Fatal Outcome ; Female ; Humans ; Male ; Models, Molecular ; Mutation, Missense ; Ornithine Carbamoyltransferase ; chemistry ; genetics ; Ornithine Carbamoyltransferase Deficiency Disease ; enzymology ; genetics ; pathology ; Pedigree ; Polymorphism, Single-Stranded Conformational ; Protein Structure, Secondary

This study is to investigate the effect of phenylhexyl isothiocyanate (PHI), which has been proved to be a novel histone deacetylase inhibitor (HDACi) recently, on gene p15 de novo expression in acute leukemia cell line Molt-4, and to further study its potential mechanism. Modified methylation specific PCR (MSP) was used to screen p15-M and p15-U mRNA. DNA methyltransferasel (DNMT1), 3A (DNMT3A), 3B (DNMT3B) and p15 mRNA were measured by RT-PCR. P15 protein was detected by Western blotting. Hypermethylation of gene p15 was reversed and activation transcription of gene p15 in Molt-4 was de novo after 5 days exposure to PHI in a concentration dependent manner. DNMT1 and DNMT3B were inhibited by exposure to PHI for 5 days (P < 0.05). Alteration of DNMT3A was not significant. It is showed that PHI could reverse hypermethylation of gene p15 and transcriptional activation of gene p15 is de novo by PHI. It may result from down-regulating DNA methyltransferases, DNMT1 and DNMT3B, or up-regulating the histone acetylation that allows chromatin unfolding and the accessibility of regulators for transcriptional activation in the p15 promoter.
Cell Line, Tumor ; Cyclin-Dependent Kinase Inhibitor p15 ; genetics ; metabolism ; DNA (Cytosine-5-)-Methyltransferases ; genetics ; metabolism ; DNA Methylation ; Histone Deacetylase Inhibitors ; pharmacology ; Humans ; Isothiocyanates ; pharmacology ; Precursor T-Cell Lymphoblastic Leukemia-Lymphoma ; genetics ; metabolism ; pathology ; RNA, Messenger ; metabolism ; Repressor Proteins ; genetics ; metabolism

Objective To investigate and compare the effects of jejunal interposition and Roux-en-Y surgery after total gastrectomy. Methods The clinical data of 41 patients with gastric cancer who underwent total gastrectomy in our hospital from June 2013 to June 2016 were retrospectively analyzed.According to the different ways of reconstruction of digestive tract,the patients were divided into observation group and control group.Patients of the observation group were with jejunal interposition surgery,while patients of the control group were trea-ted with Roux-en-Y surgery,and the effects of the two groups were compared and analyzed.Results There was no significant difference be-tween the two groups in the time of reconstruction,length of hospital stay and the incidence of complications(P>0.05).One year after sur-gery,incidence of complications,albumin,total health score,fatigue score,and disgust score of the observation group were significantly better than those of the control group with statistically significant difference(P<0.05).There was no significant difference between the two groups in survival rate,hemoglobin,total protein,weight changes(P>0.05).Conclusion For patients with total gastrectomy,jejunal interposition and Roux-en-Y surgery had similar effect.But in terms of the long-term effect,jejunal interposition can reduce complications and improve lev-el of nutrition and quality of life.

Objective To investigate the effects of colistin methanesulphonate (CMS) on the immune function of rat peritoneal macrophages.Methods The cell viability of macrophages in rat peritoneal macrophages suspension (as negative control group) and those treated with 25,50,100,125,250,500 μg · mL-1 CMS for 24 h was measured by MTI colorimetry.The secretion level of tumor necrosis factor-α (TNF-oα)was quantified by enzyme-linked immunosorbent assay after macrophages were treated with 50,125 μg · mL-1 CMS for 3,6,12,24h.The phagocytosis of macrophages was calculated by microsphere-based immunofluorescence assay,after incubation with 50,125 μg · mL-1 CMS for 3,6,12 h.Results The cell viability were 118.46％,124.72％,122.54％,46.64％,13.06％ when macrophages were exposed to CMS 25,50,100,250,500 μg · mL-1,the differences were statistically significant compared with negative control group 100.00％ (P ＜ 0.01);the cell viability of 125 μg · mL-1 CMS was 102.60％ and the difference had no significant compared with negative control group (P ＞ 0.05).After administration 3h,the secretion level of TNF-oα of negative control group and CMS 125 μg · mL-1 dose group were 32.51 pg · mL-1,56.09 pg · mL-1,the difference was statistically significant between them (P ＜ 0.05).After incubation 6h,the percentage of phagocytosis of negative control group and CMS 50,125 μg · mL-1 dose groups were 70.93％,82.03％,80.80％ and the differences were statistically significant compared to negative control group respectively (P ＜ 0.01).Conclusion CMS could improve the immune function of macrophages and exhibit immunomodulatory by increasing viability of macrophages,promoting secretion of cytokines and enhancing phagocytosis of macrophages.

OBJECTIVETo investigate the effects of chronic stress on spatial cognitive ability in different sex mice.

METHODSThirty-two adult KM mice were divided into four groups (n=8): male control and chronic stress group, female control and chronic stress group. We used the modified Kaz's methods to build on the chronic stress model of mice, and then used the place navigational testing and the probe trial testing by the Morris water maze to measure the spatial cognitive ability of mice.

RESULTSFollowing two weeks stress treatment, in the place navigational testing, to male group, the average latency to find the platform in water maze of chronic stress group was longer than that of the control; to female group, the average latency of chronic stress group was shorter than the control. Moreover, the male stress group showed faster swimming speed but longer latency to find the platform. In the probe trial testing the female chronic stress group spent more time in the target quadrant compared to the male chronic stress group.

CONCLUSIONTwo weeks' chronic stress could impair male mice's spatial cognitive ability, but improve the female's.

Animals ; Brain ; physiology ; Cognition ; physiology ; Female ; Male ; Maze Learning ; Mice ; Sex Factors ; Stress, Physiological ; physiology