Objective To observe efficacy of treatment comdyloma acuminatum with CO2 laser. Methods A local infiltration anesthesia was engaged in 29 cases of females with genital pointel condyloma and 3cases of males with anus pointed condyloma,and a compression anesthesia was conducted on the penis root of 31 cases of males with genital pointed condyloma,CO2 laser with 5 ～ 15 W output power was focused on skin lesion until it was singed then to carbon or gas. Results 24 cases of females recover,29cases of males recover,the cure rate was 84%. Conclusion CO2 laser is one basic cure for condyloma acuminatum sufferers.

The paper aims to study the pharmacokinetic parameters of gastrodin in rats effected by compound compatibilitiy and different doses of Tiangou Jiangya capsule. The extracts from Gastrodiae Rhizoma( equivalent to gastrodin 16.82 mg x kg(-1) and Tiangou jiangya capsule (equivalent to gastrodin 8.410, 16.82, 33.64 mg x kg(-1)) were oral administrated to rats respectively. The plasma were taken at various time points and treated with acetonitrile to measure the contents of gastrodin by HPLC method. The mean plasma concentration-time data were analyzed by 3P97 pharmacokinetic software and the pharmacokinetic parameters between groups were treated by SPSS 16.0. The results showed that gastrodin in rat was fitted to one-compartment model, Cmax and AUC of Tiangou Jiangya capsule were in direct proportion to oral administration, and t1/2Ka had nothing to do with doses, which indicated that gastrodin was fitted first-order rate transfter process in vivo. Morever, comparison with the Gastrodiae Rhizoma extract, isodose gastrodin in Tiangou Jiangya capsule showed a significant decrease for Cmax, Ke and increase for t1/2Ke, V/Fc, this indicated that compound compatibility can delay the absorbtion of gastrodin, prolong the resident time and promote the distribution in vivo, but its bioavailability is not significantly effected.
Administration, Oral ; Animals ; Benzyl Alcohols ; administration & dosage ; chemistry ; pharmacokinetics ; pharmacology ; Blood Pressure ; drug effects ; Female ; Flavonoids ; chemistry ; pharmacology ; Furans ; chemistry ; pharmacology ; Gastrodia ; chemistry ; Glucosides ; administration & dosage ; chemistry ; pharmacokinetics ; pharmacology ; Lignans ; chemistry ; pharmacology ; Male ; Rats ; Rats, Sprague-Dawley ; Software

Objective To explore the current status of sexual education and real needs of college freshmen in Kunming.Methods By applying random sampling,350 students from three universities in Kunming were investigated by seff-administered questionnaire,and 310 valid questionnaires were recovered.Fesults 23.9％ students confirmed that the schools do not provide courses related to sexual health,and 22.9％ of the surveyed students claimed that they even did not heard any sexual education courses in their schools.The main sources of students to acquire information in terms of sexuality and sexual health are network television,video magazines,friends and classmates.Both male and female students are more acceptable to carry out sex education lectures.There were significant differences between male and female students in the form of obtaining sexual education,the content of sexual education and the demand of sexual education in three aspects (P＜0.05).Girls tend to carry out sexual education in the form of major courses,but boys tend to the form of playing video or distributing books and periodicals (P＜0.05).Girls tend to obtain the courses on sexually disease,AIDS,heterosexual intercourse,or love psychology,but boys tend to contraceptive knowledge and skilIs,or Sexual value orientation (P＜0.05).Girls are more likely to acquire the knowledge of masturbation,sexual health,or sexual health,but boys prefer to the knowledge of sexual psychology,parasexuality,or homosexuality (P＜0.05).Conclusion As a main body of educational institute for young people,all universities should provide training courses related to sexuality and sexual health at the early stage of higher education in order to equip college students with essential knowledge and skills to have safer and positive impact on their college studies.

Adult ; Aged ; Benzamides ; therapeutic use ; Case-Control Studies ; Female ; Humans ; Imatinib Mesylate ; Immunoglobulins ; metabolism ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive ; drug therapy ; immunology ; Male ; Middle Aged ; Piperazines ; therapeutic use ; Pyrimidines ; therapeutic use ; T-Lymphocyte Subsets

Objective To investigate the sorts and quantities of air fungi in air and its change in every month in the west of Luzhou City. Methods The experiment glass slice was exposed every day and the experiment container was exposed every month in one year so as to investigate air fungi in Luzhou Library Building in the west of Luzhou City. The fungi were separated and cultured, the specimens of fungi were pressed on glass slice timely, dyed with methyl blue and identified. Results 365 experiment glass slices were exposed every day in one year. 24 139 fungi spores were checked and 23 582 were identified. They belonged to 24 species. The air fungi spores in the experiment glass slice according to their quantity were Alternaria(30.61%), Ustilaginales(23.90%), Epicoccum(18.71%), Hormodendrum(13.91%). The air fungi in the experiment container according to their quantity were Saccharomyces(50.20%), Aspergillus(12.46%), Penicillium(10.77%), Hormodendrum(9.29%) and so on. There were a lot of air fungi in the west Luzhou City all the year round. The air fungi quantity was high during May to September in a year. Conclusion A lot of fungi wave to and fro in air in the west of Luzhou City all the year round. The quantity of air fungi changes as the season goes on and it is high during May to September. The main fungi are Alternaria, Ustilaginales, Epicoccum, Hormaodendrum and so on.

Objective To investigate the effects of deguelin on the proliferation inhibition and apoptosis induction in RPMI-8226 cells in vitro,as well as the regulation of steroid receptor coactivator-3(SRC-3)to explore the relationship between them.Methods The effect of deguelin on the growth of RPMI-8226 cells was studied by MTT assay.Apoptosis was detected through Hoechst 33258 staining and Annexin-V FITC/PI double-labeled cytometry.RT-PCR Technology was applied to assessment of the mRNA expression of SRC-3,whereas,SRC-3 protein expression and localization were determined by using immunohistochemistry method.Results Deguelin presented striking proliferation inhibition potency on RPMI-8226 cells in vitro and apoptosis induction activity in a time-and dose-dependent manner.The IC50 value for 24 h was(54.55?0.40)nmol/L,(17.04?0.73)% RPMI-8226 cells went apoptotic when treated with 25 nmol/L deguelin for 24 h,with the dose of deguelin increasing to 100 nmol/L,(63.57?1.36)% cells were apoptotic.Over-expression of SRC-3 was found in RPMI-8226 cells,whereas the mRNA and protein expression levels of SRC-3 were significantly downregulated in RPMI-8226 cells induced by deguelin in a dose-dependent manner.The disposition of SRC-3 was situated mainly at the nuclear,occasionally in the cytoplasm.Conclusion Deguelin exhibited potent proliferation inhibition to the human myeloma cell line RPMI-8226 cells,furthermore,deguelin might induce RPMI-8226 cells apoptosis in a dose-dependent manner,which might correspond to the regulation of the expression of SRC-3.

Objective To observe the expression of mitochondrial encoding cytochrome oxidase subunitⅠ(COXⅠ) and subunitⅡ(COXⅡ) in type Ⅱalveolar epithelial cell(AEC Ⅱ) of rats exposed to hyperoxia and explore the role of COXⅠand COXⅡ in hyperoxia-induced lung injury.Methods AECⅡ were gained by primary culture from 19-days fetal rats lung.After purified,AECⅡ were randomly assigned to hyperoxia group and air group.Hyperoxia group was flushed the flake with 950 mL/L O2 and 50 mL/L CO2 at 3 L/min for 10 min,then sealed.Both groups were in CO2 culture chamber(37 ℃,50 mL/L CO2).After 6,12 and 24 hours of exposure,AECⅡ were harvested and extracted for total RNA.COXⅠand COXⅡ mRNA were detected by reverse transcription polymerase chain reaction(RT-PCR).The results were analyzed by SPSS 12.0 softwore.Results Compared with air group,COXⅠmRNA expression in hyperoxia group increased significantly at 6 hours(t=3.832 P=0.019) and 12 hours(t=10.431 P=0),respectively,then decreased to the equivalent level in 24 hours(t=0.360 P=0.731).Compared with air group,COXⅡmRNA expression in hyperoxia group increased significantly at 6 hours(t=2.795 P=0.035),then decreased to the equivalent level at 12 hours(t=0.892 P=0.40) and 24 hours(t=2.018 P=0.071).Conclusions Exposure of hyperoxia up-regulate the expressions of COXⅠmRNA and COXⅡmRNA in AECⅡ,which may be a protective mechanism of hyperoxia-induced lung injury.

Objective To investigate the efficacy and safety of fludarabine plus mitoxantrone combination chemotherapy for malignant lymphoma compared with CHOP regimen.Methods Sixty-six patients with malignant lymphoma were divided into fludarabine group and CHOP group.The efficacy and toxicities of these two regimens were analyzed and compared between the two groups.Results The overall response(OR)was 81.5% in fludarabine group,while was 48.7% in CHOP;for the patients in late-stage,with B cell lymphoma,with T cell lymphoma and with high serum LDH level,the OR in fludarabine group was higher than in CHOP group obstructively,being 62.5% and 35.7%.Conclusion Fludarabine combination chemotherapy,as a first line regimen in treating first-treated lymphoma,recrudescent lymphoma,intractable lymphoma and lymphoma in late stage,possesses higher efficacy,safety and tolerance than CHOP regimen.

Background The novel dynamic biomechanical device Corvis ST can measure a variety of corneal deformation parameters and record the dynamic process of cornea deformation.Understanding various corneal deformation parameters is of important clinical significance for morphological and functional prediction for myopic eyes in corneal refractive surgery.However,the range and meaning of corneal deformation parameters in myopic eyes are still unknown.Objective This study was to establish the range of corneal defamation parameters by Corvis ST and explore its clinical significance in myopic eyes.Methods A descriptive study was designed under the approval of Ethic Committee of Tianjin Eye Hospital and informed consent of patients.This study protocol complied with Helsinki Declaration.Four hundred and seventy-seven eyes of 257 myopic patients who were going to receive corneal refractive surgery were included in Tianjin Eye Hospital from June 2014 to January 2015.The corneal deformation parameters including the first applanation time (1st A-time),first applanation lengh (1 st A-length),first applanation velocity (1 st A-velocity),second applanation time (2nd A-time),second applanation length (2nd A-length),second applanation velocity (2nd A-velocity),highest concavity time (HC-time),highest concavity deformation amplitude (HC-DA),highest concavity peak distance (HC-PD),highest concavity radius (HC-R),non-contact intraocular pressure (IOPnct) and the central corneal thickness (CCTst) were measured by Corvis ST.Corneal structural parameters including CCT and corneal curvature were measured by 3-D anterior segment analysis system (Pentacam),and corneal biomechanical parameters including corneal resistance factor (CRF),corneal hysteresis (CH),intraocular pressure mimic Goldmann (IOPg) and intraocular pressure of corneal compensation (IOPcc) were measured by Ocular Response Analyzer (ORA).The distributions of the data were tested with the Shapiro-Wilk test and 95％ confidence intervals (CI) were calculated.The correlations of the variables were evaluated by Pearson correlation coefficient and Spearman rank correlation coefficient.Multiple linear regression was used to analyze the changes of 1st A-time,2nd A-time and HC-DA with corneal morphology and biomechanical parameters.Resnlts Only 1st A-time,2ndA-time,HC-time,HC-DA,CCTst and IOPnct showed the normality distribution in corneal deformation parameters.The positive correlations were found between 1st A-time,1st A-length,2nd A-length,2nd A-velocity,HC-R with CCT (r=0.338,rs=0.129,rs=0.282,rs =0.374,r=0.306,all at P＜0.01),while 1st A-velocity,2nd A-time,HC-D and HC-PD showed the negative correlations with CCT (rs =-0.235,r=-0.130,r=-0.259,r=-0.226,all at P＜0.01).CRF and CH showed positive correlations with 1st A-time,2nd A-length,2nd A-velocity,HC-time,HC-R and negative correlations with HC-PD,△A-length (all at P＜0.05).1st A-time,2nd A-time and HC-DA changed with CRF and IOPcc with the regression formations of 1st A-time =6.185+0.066CRF+0.034IOPec (F=300.123,P =0.000),2nd A-time=23.397-0.074IOPec-0.044CRF (F=227.979,P=0.000) and HC-DA =1.523-0.017IOPcc-0.017CRF (F=152.662,P=0.000).The CCTst,IOPst and IOPnct values by Corvis ST were significantly lower than CCT by Pentacam ([548.23±26.31] μm,IOPg ([15.02±2.72]mmHg and ([16.02-±2.56]mmHg) by ORA respectively (t =11.00,2.919,6.815,all at P ＜ 0.01).Conclusions The corneal deformation parameters by Corvis ST can quantitatively describe corneal biomechanical properties in myopic eyes.Both IOP and the corneal steep curvature of the 3 mm center area (K2) affect the deformation response of cornea.The reliability of Corvis ST in the measurement of CCT and IOP remains to be further studied.

Objective To investigate the diagnosis and treatment of sequential diffuse large B-cell lymphoma (DLBCL) after peripheral T-cell lymphoma (PTCL).Methods A case with sequential DLBCL after PTCL was reported,and the characteristics and responses of this case were analyzed.The previous literature was reviewed in order to explain the mechanism and prognosis of such type of disease.Results This patient was diagnosed as PTCL not otherwise specified (PTCL-NOS) definitely,but after a period of treatment,DLBCL was developed as a second tumor.The characteristics and onset interval were just similar to those described in the literature,in which the mechanisms were mentioned as common effects of tumor cell,microenviroment and therapies.This patient got effects through the initial treatment,but considering the poor outcome by former researchers,the prognosis needed to be closely followed up.Conclusion Sequential development of EBV-unrelated DLBCL after PTCL-NOS is very rare,and the mechanism,therapy and prognosis need further investigation.