Objective To study the influence of different eyedroping interval on the effects of mydriasis.Methods Divided the selected patients into the experiment group(126 cases) and the control group(128 cases) randomly.Different eyedroping interval were adopted in the different group,and then compared the datum which obtained from 2 groups.Results The diameter of pupils in the experiment group were 7.45 mm and the 7.48 mm respectively after the mydriasis,which in the control group were 6.96 mm and 7.00 mm respectively,the difference between the 2 groups was significant.Conclusion Using eyedrop of tropicamide by every 5 minutes can effective shorten the operation time and can obtain better effects than the routine method.

Objective To design the meta-data scheme for medical records database of traditional Chinese medi-cine.Methods The meta-data scheme for medical records database of traditional Chinese medicine was designed u-sing the MARC meta-data according to the analysis of resources, investigation of literature, networks and users. Results The meta-data scheme for medical records database of traditional Chinese medicine is consisted of 17 fields and 9 parts.Conclusion MARC, as the mata-data criteria, can organize and describe the medical records of tradi-tional Chinese medicine.However, the standards for selection and description of meta-data criteria need to be fur-ther studied.

Objective: To establish the yeast expression system for oncostatin M (OSM). Methods:The cDNA encoding human OSM was inserted into pPIC9K vector and transformed into Pichia pastoris. The correct phenotype of recombinants was screened through MD and MM auxotrophic culture medium. The OSM mRNA transcription in the P.pastoris and molecular weight were identified with Northern blot and Western blot. MTT method was used to test the activity of OSM in inhibiting the growth of human melanoma cells. Results: Established P.pastoris system could accomplish human OSM secreting expression. The recombinant OSM with a 30 000 molecular weight amounted to 70% of the total protein in medium supernatant. The OSM showed an inhibitory effect on the growth of A375 cells in vitro. Conclusions：The P.pastoris system of OSM was proved effective with a high expressing level. The secreted recombinant OSM present in supernatant of culture medium would be easy to be purified. It may lay the foundation for the large-scale preparation of OSM in future.

Angiopoietins ; metabolism ; physiology ; Animals ; Cell Proliferation ; Endothelial Cells ; cytology ; physiology ; Humans ; Neoplasms ; blood supply ; Neovascularization, Pathologic ; physiopathology ; Neovascularization, Physiologic ; physiology ; Pericytes ; cytology ; metabolism ; physiology ; Proto-Oncogene Proteins c-sis ; metabolism ; physiology ; Receptor, Platelet-Derived Growth Factor beta ; metabolism ; physiology ; Receptor, TIE-2 ; metabolism ; physiology ; Signal Transduction

Objective:To establish the method of determination of the contents of Danshensu and Protocatechuic aldehyde in Huoxue Mixture simultaneously by RP HPLC.Methods:The determination was carried out with Inertsil ODS column. The mobile phase consisted of methanol 0.5% HAc (22∶78) and UV detection wavelength was 280nm. Results:The average recovery of standard addition were 96.7%, RSD =1.12% ( n =5) for Denshensu, and 97.0 %, RSD =1.53% ( n =5) for Protocatechuic aldehyde, There was a good linear relationship between the absorption area value and the concentration in the range of 1.25～10?g for Deshensu and 0.25?g～2.0?g for Protocatechuic aldehyde.Conclusion: The method is simple, acurate, reproducible and can be used for content determination of Huoxue Mixture.

Objective To investigate the effect of different immunosuppresants on CAN by affecting TGF-?_ 1 and Smads signal pathway. Methods The SD→Wistar rat accelerated kidney sclerosis model was made following the procedure of Kamada with our modification. The rats were divided into 6 groups (each group n=8): Group A, psudo-OP group; Group B, control group; Group C, CsA 6 mg?kg~ -1?d~ -1; Group D, FK506 0.15 mg?kg~ -1?d~ -1; Group E, MMF 20 mg?kg~ -1?d~ -1; Group F, RPM 0.8 mg?kg~ -1?d~ -1. After 4, 8, 12 weeks, the animals were killed. The immunohistochemistry and real-time fluorescence quantitative PCR were used to detect localization and expression of TGF-?_ 1, Smad2 and smad7 in transplant kidney. Results CsA and FK506 simultaneously stimulated the gene expression and protein production of TGF-?_ 1 and Smad2 and inhibited the expression of Smad7 in transplant kidney, whereas MMF and RPM could down-regulate the gene expression and protein production of TGF-?_ 1 and smad2 and up-regulate the expression of smad7. There was no significant difference between CsA group and FK506 group, as well as MMF group and RPM group.Conclusion Different immunosuppresants can affect TGF-?_ 1 and Smads signal pathway in kidney graft. CsA and FK506 can cause CAN, owning to up-regulate the expression of Smad2, and down-regulate the Smad7 expression. MMF and RPM can prevent the progression of CAN because of down-regulating the expression of Smad2 and increasing Smad7 production.

Child ; Cord Blood Stem Cell Transplantation ; adverse effects ; Cystitis ; therapy ; Hemorrhage ; therapy ; Humans ; Mesenchymal Stem Cell Transplantation ; adverse effects ; Postoperative Complications ; therapy

Objective:To establish the determination of the contents of ferulic acid and paecniflorin in Huoxue Mixture Radix Salviae Miltiorrmizae, Radix Angelicae sinensis, Radix Paeoniae Rubra cortex Moutan, Radix Astragali and caulis Spatholobi simultaneously by HPLC.Methods:The determination was carried out with ZORBAX *ODS column. Chromatographic condition of ferulic acid: the mobile phase consisted of methanol 1%HAc (42∶58) and UV detection wavelength was at 313nm. Chromatographic condition of paecniflorin: the mobile phase consisted of methanol water (25∶75) and UV detection wavelength was 230nm.Results:There was a good linear relationship between the absorption peak area and the concentration in the rang of 0.125～1.0?g for ferulic acid and 0.25～2.0?g for paecniflorin, respectively.Conclusion:The method is simple, acurate, reproducible and can be applied for content determination of Huoxue Mixture.

Objective: To explore the influence of Aiweixin on cardiocyte apoptosis of myocardial ischemia-reperfusion injury in rats. Methods: Thirty male Wistar rats, 200-250g weight, were divided into 3 groups randomly: sham-operation group, Ischemia-reperfusion injury group (I/R) and Aiweixin preconditioning group, each group had 10 rats. The model of myocardial ischemia-reperfusion injury was established. The protein expressions of Bcl-2, Bax and Caspase-3 were studied by Immunohistch emicalstaining. Results:The protein expressions of Bax and Caspase-3 increased and Bcl-2 decreased signifi cantly (P

BACKGROUND:It has been proved that Rhizoma Chuanxiong can promote osteoblast proliferation and differentiation.
OBJECTIVE:To further observe the effects of tetramethylpyrazine (Ligustrazine) extracted from Rhizoma Chuanxiong on osteoblast proliferation and type I col agen expression.
METHODS:Mouse osteoblast cel line MC3T3-E1 was cultured in medium containing 1, 5, 10, 15 and 20 mg/L Ligustrazine, respectively. Osteoblast proliferation was detected by cel counting kit-8 assay, and alkaline phosphatase activity and type I col agen level measured using ELISA method.
RESULTS AND CONCLUSION:Different concentrations of Ligustrazine al significantly promoted the osteoblast proliferation and type I col agen level compared with the blank control group (P<0.005). The activity of alkaline phosphatase in al Ligustrazine groups except 15 and 20 mg/L groups was significantly higher than that in the blank control group (P<0.005). These results show that Ligustrazine isolated from Rhizoma Chuanxiong can effectively promote the osteoblast proliferation and type I col agen expression, with the optimum concentration of 10 mg/L.