Objective To study the influence of different eyedroping interval on the effects of mydriasis.Methods Divided the selected patients into the experiment group(126 cases) and the control group(128 cases) randomly.Different eyedroping interval were adopted in the different group,and then compared the datum which obtained from 2 groups.Results The diameter of pupils in the experiment group were 7.45 mm and the 7.48 mm respectively after the mydriasis,which in the control group were 6.96 mm and 7.00 mm respectively,the difference between the 2 groups was significant.Conclusion Using eyedrop of tropicamide by every 5 minutes can effective shorten the operation time and can obtain better effects than the routine method.

Objective To design the meta-data scheme for medical records database of traditional Chinese medi-cine.Methods The meta-data scheme for medical records database of traditional Chinese medicine was designed u-sing the MARC meta-data according to the analysis of resources, investigation of literature, networks and users. Results The meta-data scheme for medical records database of traditional Chinese medicine is consisted of 17 fields and 9 parts.Conclusion MARC, as the mata-data criteria, can organize and describe the medical records of tradi-tional Chinese medicine.However, the standards for selection and description of meta-data criteria need to be fur-ther studied.

Objective: To establish the yeast expression system for oncostatin M (OSM). Methods:The cDNA encoding human OSM was inserted into pPIC9K vector and transformed into Pichia pastoris. The correct phenotype of recombinants was screened through MD and MM auxotrophic culture medium. The OSM mRNA transcription in the P.pastoris and molecular weight were identified with Northern blot and Western blot. MTT method was used to test the activity of OSM in inhibiting the growth of human melanoma cells. Results: Established P.pastoris system could accomplish human OSM secreting expression. The recombinant OSM with a 30 000 molecular weight amounted to 70% of the total protein in medium supernatant. The OSM showed an inhibitory effect on the growth of A375 cells in vitro. Conclusions：The P.pastoris system of OSM was proved effective with a high expressing level. The secreted recombinant OSM present in supernatant of culture medium would be easy to be purified. It may lay the foundation for the large-scale preparation of OSM in future.

Angiopoietins ; metabolism ; physiology ; Animals ; Cell Proliferation ; Endothelial Cells ; cytology ; physiology ; Humans ; Neoplasms ; blood supply ; Neovascularization, Pathologic ; physiopathology ; Neovascularization, Physiologic ; physiology ; Pericytes ; cytology ; metabolism ; physiology ; Proto-Oncogene Proteins c-sis ; metabolism ; physiology ; Receptor, Platelet-Derived Growth Factor beta ; metabolism ; physiology ; Receptor, TIE-2 ; metabolism ; physiology ; Signal Transduction

ObjectiveTo observe the clinical effect of Chinese medication plus warm needling in regulating the hypothalamo-pituitary-ovarian axis (HPOA) for anovulatory infertility.MethodThe relevant clinical data of sixty-seven women with infertility were comprehensively analyzed, to summarize the risking factors for anovulatory infertility, and then the subjects were randomized into an observation group and a control groupby their visiting sequence. The control group was intervened by orally taking Bushen Tiaojing Cupailuan (tonifying kidney, regulating menstruation, and promoting ovulation) decoction, while the observation group was by warm needling in addition to taking the same decoction. The clinical efficacies, growth of endometrium, and size of follicle were compared between the two groups.ResultWarm needling plus Bushen Tiaojing Cupailuan decoction produced a positive effect in treating anovulatory infertility, andthe pregnancy rate of the observation group was significantly higher than that of the control group (P<0.05).ConclusionThe effect produced by warm needling plus Bushen Tiaojing Cupailuan decoction has a comparatively high clinical value.

Hyperhomocysteinemia is an independent risk factor for arterial and venous obstructive vascular di-seases. Raised plasma homocysteine concentrations are caused by genetic and non-genetic factors. The pathogenic mechanism is not clear. It may be associated with endothelium dysfunction, abnormal lipid metabolism, hypertrophy of intima media, increased platelet aggregation and several clotting factors interfered, etc. The metabolism and pathogenic mechanism of homocysteine are reviewed in this article, which provides theoretical foundation for the treatement of hyperhomocysteinemia.

Objective To observe morphological character of posterior arch of Atlas and lamina of axis,as well as provide anatomical basis to design special guiding device for the operation of upper cervical posterior fixation with Atlas titanium cable so as to make the operation safe and easy.Methods The posterior arch of Atlas and lami- na of axis were observed and measured in 100 adult dry Atlas and axis specimens,then special guiding devices were designed based on the anatomical data.Results (1)The posterior arch of Atlas was thin with a fan-shape section, whose inner plane descended from back to inside and the lamina of axis was stronger and thicker than posterior arch of Atlas,with a vertical inner plane and a ridge near the lower edge.(2)The special guiding device for posterior arch of Atlas should be designed with a bend head as well that for lamina of axis should be designed with a round head. They were used in 61 operations which became safer and easier.Conclusion The operation of upper cervical posteri- or fixation with Atlas titanium cable would be safer with the help of special guiding devices which were designed based on the anatomical character.

Objective:To establish the method of determination of the contents of Danshensu and Protocatechuic aldehyde in Huoxue Mixture simultaneously by RP HPLC.Methods:The determination was carried out with Inertsil ODS column. The mobile phase consisted of methanol 0.5% HAc (22∶78) and UV detection wavelength was 280nm. Results:The average recovery of standard addition were 96.7%, RSD =1.12% ( n =5) for Denshensu, and 97.0 %, RSD =1.53% ( n =5) for Protocatechuic aldehyde, There was a good linear relationship between the absorption area value and the concentration in the range of 1.25～10?g for Deshensu and 0.25?g～2.0?g for Protocatechuic aldehyde.Conclusion: The method is simple, acurate, reproducible and can be used for content determination of Huoxue Mixture.

Objective:To establish the determination of the contents of ferulic acid and paecniflorin in Huoxue Mixture Radix Salviae Miltiorrmizae, Radix Angelicae sinensis, Radix Paeoniae Rubra cortex Moutan, Radix Astragali and caulis Spatholobi simultaneously by HPLC.Methods:The determination was carried out with ZORBAX *ODS column. Chromatographic condition of ferulic acid: the mobile phase consisted of methanol 1%HAc (42∶58) and UV detection wavelength was at 313nm. Chromatographic condition of paecniflorin: the mobile phase consisted of methanol water (25∶75) and UV detection wavelength was 230nm.Results:There was a good linear relationship between the absorption peak area and the concentration in the rang of 0.125～1.0?g for ferulic acid and 0.25～2.0?g for paecniflorin, respectively.Conclusion:The method is simple, acurate, reproducible and can be applied for content determination of Huoxue Mixture.

Alzheimer′s disease(AD)is a neurodegenerative disorder resulted from complicate interactions between genes and environment. There is no effective therapy so far. The genome-wide association study(GWAS)provides the opportunity to discover the risk genes of sporadic AD,which is informative for revealing the pathogenesis of AD and guiding new drug development. In this re?view,we summarize the current findings of genetic studies of AD,the risk genes and their biological relevance with AD,and new drug development strategy supported by genetic studies.