Mutations in human mitochondrial tRNA genes are responsible for a variety of human inherited diseases. Investigations of the molecular mechanisms of these diseases are of great interest for nowadays scientists. According to the study of post-transcriptional modification patterns of human mitochondrial tRNAs, novel taurine-containing modifications were identified at the anticodon wobble nucleotides of mitochondrial tRNALeu(UUR) and tRNALys. Recently, it was reported that mitochondrial tRNAs harboring one of those encephalomyopathies related mutations, such as A8344G, A3243G, T3271C etc., lacked the normal taurine-containing modification at their anticodon wobble positions. Wobble modification deficiencies of mutant mitochondrial tRNAs were found from cybrid cells, as well as from patient tissues. Molecular surgery experiments showed that the wobble modification is essential for the interaction between the anticodon in tRNA and the codon in mRNA. Furthermore, the enzyme that is responsible for the formation of the modification was identified and characterized. These studies strongly suggested a key molecular factor responsible for the inherited mitochondrial encephalomyopathies and could potentially lead to the development of a gene therapy for these diseases.

Objective:To establish the pathological sample database and standardize pathology specimen management processes to provide a platform for the study of pathology samples. Methods: Using J2EE, JSP, and component object model technology, the system establish the pathological sample library work platform for the pathology network resources, documentation, and intelligent. Results:The system established pathology samples and workflow management system architecture for the collection, storage and analysis of pathology specimens. Conclusion:The system designed to enhance the authenticity and credibility of the sample test results, thereby improve the accuracy of the sample analysis and provides a good reference for information technology-related departments.

Platelets, small pieces of cytoplasm with biological activity, split and fall off the megakaryocytes and mature from the bone marrow. After stimulated, platelets produce nitric oxide to inhibit their own activation and aggregation. Pathologically, the injury of endothelial cells activates platelets and changes their functions. The release of inflammatory mediators and cytokines induces and enhances the development and progression of atherosclerosis, and thereby promotes the occurrence of erectile dysfunction. Besides, platelets and their related functional parameters may serve as important indicators in the diagnosis and treatment of erectile dysfunction.
Atherosclerosis ; etiology ; Blood Platelets ; physiology ; Cytokines ; metabolism ; Endothelial Cells ; Erectile Dysfunction ; etiology ; Humans ; Male ; Nitric Oxide ; biosynthesis ; Platelet Activation

Objective To explore the causes and treatments for complications of laparoscopic incisional hernia repair.Methods A total of 110 patients with incisional hernia(42 men and 68 women,aged 33 to 89 years with a mean of 65)were treated with laparoscopic mesh repair in our hospital from March 2004 to May 2007.The clinical records of the patients were analyzed retrospectively.Results The laparoscopic repair was completed in all of the patients expect in one,who was converted to open surgery because of intraperitoneal adhesion.After the operation,15(13.6%)patients developed abdominal distension,86(78.2%)had severe pain in the operative area(not less than 6 weeks in 2 cases),23(20.9%)had seroma,6(5.4%)had urinary retention,2(1.8%)had acute gastric dilation,2(1.8%)had intestinal fistula,2(1.8%)had respiratory dysfunction,and 2(1.8%)showed recurrence of the hernia.Conclusion Most of the complications of laparoscopic incisional hernia repair are caused by insufficient preoperative preparation,intraperitoneal adhesion,difficulty in exposure of the visual field,improper use of electrocoagulation,small-sized masses,or weak fixation.

Objective To investigate the effectiveness of laparoscopic mesh repair of parastomal hernia. Methods Laparoscopic mesh repair was performed in 7 patients with parasromal hernia from September 2004 to December 2005 in this hospital.Results Laparoscopic mesh herniorrhaphy was successfully performed in 6 patients, while a conversion to open herniorrhaphy was required in 1 patient because of extensive intraperitoneal adhesion. The operating time was 45~180 mi (mean, 109 min), and the hernial diameter was 4~6 cm (mean, 5.6 cm). Transient abdominal distention happened in 2 patients. Five patients complained of a pain in operative area within 3 weeks. Seroma occurred in 4 patients and was cured by needle aspiration and pressure bandaging for 2~ 4 times. No hematoma or surgery-related infection was seen. The length of postoperative hospital stay was 3~8 d (mean, 5.1 d). Postoperative follow-up for 2~15 months (mean, 8.3 months) revealed no recurrence. Conclusions Laparoscopic mesh repair of parastomal hernia is safe, effective, and feasible.

Objective To investigate the relationship between exposure intensity and illumination time of blue light and replicative senescence of rat retinal pigment epithelial (RPE) ceils.Methods Thirty-six 12-14 weeks Wistar rats were kept in the cage with a blue-light bulb [(450±10) nm],and were randomly divided into four groups (no light,nature light,500 lx light and 1000 lx light illumination),each has nine rats.The rats in each group were further divided into three subgroups according to illumination time (one month,two months or three months).Eyeballs were collected after intraperitoneal injection of 10% chloral hydrate.The right eye of each rat was embedded in paraffin and sectioned for hematoxylin-eosin (HE)staining,while frozen sections of the left eye were stained for the senescence-associated β-galactosidase (SA-β-Gal).The data were analyzed by SPSS11.5 statistical software.Results The amounts of SA-β-Gal positive RPE cells were significantly different between all groups under the same illumination time 17 (P=0.000),and between all subgroups of different illumination time with same exposure intensity (P＜0.01)except for the control group (no light).Conclusion Blue-light can induce replicative senescence in rat RPE cells in an intensity and time-dependent manner.

Objective Our aim was to investigate the association between sudden sensorineural hearing loss (SSHL)and mean platelet volume (MPV)values,in order to evaluate the etiology of SSHL.Methods This study included 40 patients with SSHL in our ward between Jan.2012 and Jan.2014 and 30 age- and sex-matched healthy subjects were included as controls.The correlation among the levels of the number of platelets,mean plate-let volume(MPV),platelet distribution width (PDW)was evaluated in the two groups.ResuIts The platelet count was significantly lower in the SSHL than the control group(212.0±47.9 versus 237.8±46.2 ×109/L).MPV and PDW were significantly higher in SSHL(10.9±0.8 versus 10.3±0.8 fL;12.9%±2.0% versus 12.0%±1.3 %). The SSHL patients were divided two subgroups:the mild-moderate subgroup (hearing loss 25~70 dB)and the se-vere subgroup(hearing loss≥70 dB).The MPV values were insignificantly different between the two subgroups(11. 0±1.1 fL versus 10.8 ±1.0 fL).Compared to the control group,the two subgroups had both significant differ-ences in MPV.ConcIusion High MPV values are a risk factor for thrombotic vascular diseases.Considering the in-creased MPV levels,SSHL appears to be characterized by ischaemic or thrombotic events.

Adult ; Diagnostic Tests, Routine ; Female ; Hearing Loss, Sensorineural ; Humans ; Pedigree ; Thrombocytopenia ; diagnosis ; genetics

Objectives To study the effects of effective constituent of Cortex Moutan-Paeonol and Paeoniflorin on myocardial ischemia and myocardial infarction in experimental canines.Methods The coronary artery ligation was employed to establish the model of canines with acute myocardial ischemia and myocardial infarction.The extent of myocardial ischemia was detected by epicardial-electrocardiogram and the extent of infarction was determined by N-BT staining method.Results The adminstration of Paeonol and Paeoniflorin reduced the myocardial ischemia level(P

[Objective]To evaluate the outcome inforation provided by two scoring systems after operative treatment of elementary posterior wall fractures of the acetabulum.[Method]Thirty-two patients with elementary posterior wall fracture of the acetabulum were included in the study.Functional outcomes were assessed with use of the eight health dimensions of the short form(SF)-36,and clinical outcomes were evaluated with use of the modified Merle d' Aubign? score.The SF-36 scores were compared with previously reported normal values and the correlation was analyzed between the modified Merle d' Aubign? score and score of each SF-36 health dimension.[Result]The mean modified Merle d' Aubign? score was 16.28 (range 13-18,SD 1.3),indicating overall good-to-excellent clinical results.However,all SF-36 health dimensions were significatly lower than the expected forms,with a significant statistical difference(P