Adult ; Aged ; Benzamides ; therapeutic use ; Case-Control Studies ; Female ; Humans ; Imatinib Mesylate ; Immunoglobulins ; metabolism ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive ; drug therapy ; immunology ; Male ; Middle Aged ; Piperazines ; therapeutic use ; Pyrimidines ; therapeutic use ; T-Lymphocyte Subsets

Analysis the positive literature at home and abroad and understand the status by searching the CNKI, Wanfang, Cqvip Chinese databases for the descriptive analysis of the literature. At present, positive psychology is still in the theoretical and construction stage, nursing intervention model is not yet mature, but providing useful ideas and try on promoting patients to improve mental health and quality of life, for the nurse to provide theoretical basis and reference in the future.

Objective: To study the preventive actions of ganoderma lucidum polysaccharide(GLP) against kidney damage induced by cisplatin. Methods : Female Wistar rats were randomly divided into normal saline(NS) group, cisplatin(CDDP) group, GLP group, CDDP+GLP group. The changes of Scr, BUN, MDA, SOD were measured and renal structure was observed after 5 days by injecting drugs. Results : The contents of serum Scr and BUN of CDDP group were significantly highter than that of NS group. The activity of RBC SOD reduced and the contents of serum MDA increased. The contents of renocortical tissue MDA increased and the activity of SOD declined in renocortical tissue. The contents of serum Scr and BUN of GLP+CDDP group were significantly lower than that of CDDP group. The activity of RBC SOD increased and the contents of serum MDA declined. The concents of renocortical tissue MDA declined and the activity of SOD increased in renocortical tissue. The pathological slice indicated that renal structure was significantly improved. Conclusion : GLP may reduce cisplatin nephrotoxicity and its mechanism may be correlative with that GLP inhibited the blood and renocortical tissue lipid peroxidation increasing.

Objective To explore the peer assisted learning approach combined with micro-teaching in anesthesia internship for oversea students.Methods 60 international medical students were randomly divided into two groups.Students in the control group have peer guidance before routine theatre observation,while students in experimental group discuss micro-teaching of anesthetic skills with each other by watching real time videos from smart phone.Students' subjective attitudes to new approach were evaluated and their final exam score were analyzed.SPSS 1 1.0 was used for data processing and the data was made descriptive analysis,t test and analysis of variance.Results Students in experimental group did better in both theory exam (46.8 ± 4.03) vs.(35.7 ±6.12) and practice exam (35.2 ± 3.20) vs.(27.6± 4.71) than those in control group,and the total scores of two groups were (80.5 ± 7.40) and (63.1 ± 6.81) respectively.These scores all had significant difference between two groups.More students from experimental group than those from control group feel interested in anesthesiology (P＜0.01) and feel confident about exams (P＜0.05);There was no difference between two groups in understanding clinical guideline (P=0.57).Conclusion Peer assisted learning combined with micro-teaching is proved to enhance the quality of anesthesia teaching during internship for oversea undergraduates.

AIM: To investigate the influence of He-Ne laser on connective tissue growth factor (CTGF) expression and collagen formation of fibroblast in filtration site after trabeculectomy in rabbit, and to discuss the mechanism for preventing scar formation with He-Ne laserin vivo.METHODS: The upper nasal limbus area next to the upper rectus muscle in right eyes Received 10 minutes He-Ne laser irradiation (200mW/cm2) every day for three days, the left eyes served as control. Twenty-four hours after the last irradiation, both eyes of the rabbits were took trabeculectomy surgery. The expressions of CTGF in the filtration area were tested on the 7th, 14th and 28th day after surgery and collagen density was tested on the 14th and 28th day after surgery. Each of the time point had 7 rabbits. RESULTS: The expression of CTGF was lower than that of the control group's on the 7th and 14th day after trabeculectomy surgery (P=0.01, P=0.005). When examined on the 14th and 28th day, the collagen density of irradiation group were significantly lower than that of the control group's (P=0.013, P=0.01).CONCLUSION: Pretreating the filtration area with 200mW/cm2 He-Ne laser may be helpful in preventing scar formation after trabeculectomy in rabbit, possibly due to downregulation of the expression of CTGF and collagen synthesis in fibroblasts. He-Ne laser may be developed into a new scar preventing method in filtration surgery.

·AlM: To explore the treatment effect of initial lacrimal passage irrigation and probing in neonatal dacryocystitis. · METHODS: ln this retrospective case series study, 224 outpatients ( 256 eyes ) with neonatal dacryocystitis were selected from January 2012 to December 2012 in our hospital.The patients were divided into 5 groups ( 1 ~3mo, 4~60mo, 7 ~9mo, 10 ~12mo and >12mo).lnitial lacrimal passage irrigation and probing were operated, the therapeutic effect of different groups were compared. ·RESULTS:ln this study, 256 eyes of 224 cases, 242 eyes (94.5%) were cured and 14 eyes ( 5.5%) were failed. Bony nasolacrimal duct obstruction happened more obviously in children aged more than 10mo with lower cure rate.More probing was required with the children aged (Spearman rank correlation analysis, γs=0.746, P<0.01).All of the 226 eyes with Hasner valve obstruction were cured.Bony nasolacrimal duct obstruction was 30 eyes, 16 eyes were cured (the cure rate was 53.3%).The cure rate of two parts of the obstruct had significant difference.There were no cases of infection and false passage.
· CONCLUSlON: The treatment of primary lacrimal passage irrigation and probing in neonatal daryocystitisis effective and safe, the optimal timing of treatment is 1 ~9mo.With the growth of age, the cure rate is decreased.

Objective To explore the survival mechanism of hippocampal ne urons after damage of hypoxia-ischemia and reperfusion of brain.Methods Seven days old SD rats(n=56) were randomly divided into hypoxia-ischemia br a in iniury(HIBD) group and sham group.The HIBD and reperfusion model was establis hed.The flowing of blood was de tected by multicolor Doppler.The p-CREB(phosphorylated c-AMP response element bi nding protein)and c-Jun were immunohistochemically evaluated in hippocampus.Thi onin staining was used to observe the apoptosis.Results The expression of p-CREB reache d the peak at 3,24 h postreperfusion in the right hippocampus of HIBD group,and then decreased to the normal level on the 7th day.In contral group the same reg ions showed basic immn-noreactivity.While c-Jun reached the peak at 6 h postreperfusion,then with a slightly decrease at 24 h;and at 48 h the other peak appeared,then with a gradual decline .On the 7th day the mumber of positive cells were still significanthy more than control group(P0.05).The sham animal showed very few apoptosis cells in the regio ns of hippocampus.Conclusions The persistent activation of CREB in the hippocampus regulates,the expression of c-Jun through the signal transductions and is involved in the course of neuron s′ survival and repair during the period of post hypoxia-ischemia reperfusion.I t is very important for the protection of the pyramidal hippocampal neurons on t he damaged side,especially for the sensitive region CA1. J Appl Clin Pediatr,2005,20(2):133-135

With the advanced development of information technology, there is a huge impact on various industries for the arrival of big data. In the biomedical field, innovative genome sequencing technology enables low-cost, high-throughput, and high-speed to become a reality, which leads to an explosive growth in data and also appeared in an urgent need to process those massive biological information. High performance computing (HPC) along with effective methods is one of the best ways to deal with the problem of big data in biomedical field which could serve the biomedical development best. We discussed the issues faced in biomedical big data processing and concluded that the bioinformatics is an indispensable component of biomedical technologies.
Biomedical Research ; trends ; Computational Biology ; Computing Methodologies ; Humans

Caused by Helminthosporium carposaprum, tomato brown lea f spot was a serious disease in green house in Henan Province. The condition for promoting sporulation of fungi were tested in this paper. The results showed th at the number of sporulation were different on the different medium,the fungi c ould sporulate a lot on the PDA+tomato leaf and Czapek medium, but V8、PSA and t omato juice restrained sporulation.The best carbon source and nitrogen source f or the fungi promoting sporulation were fructose and ammonium chloride respectiv ely,mannitol and Peptone ammonium sulfate restrained sporulation. Light and ult raviolet radiation were in favor of sporulation , ultraviolet radiation irradiat ing for 60～80min promoted sporulation. The fungi were promoted sporulation on the condition of lower or higher temperature and alkalescence,which 15℃o r 30℃,pH 8～9.

Reverse transcriptase-PCR experiments suggest that the two clusters of genes potentially involved in the oxidation of reduced sulfur compounds are organized as operons in strain of the acidophilic, chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans ATCC 23270, the two clusters of genes including such the ORF of putative sulfate-thiosulfate-molybdate binding proteins, the ORF of putative thiosulfate: quinone oxidoreductase and the ORF of the rhodanese-like protein (P21). Bioinformatic analyses have predicted the possible promoters sequences and the possible +1 start site of transcription for the doxDA operons.