Objective: To investigate the expression ofVEGF-Aand the correlation with the sentinel lymph nodes lymphangiogenesis and its metastasis in non-small cell lung cance(rNSCLC).Methods: The expression of VEGF-A was detected in 65 patients with NSCLC by SP immunohistochemistay. The expression of LYVE-1 was detected by immunofluorescence. The new lymphatic vessel density was counted by LYVE-1 and analyzed with clinicopathologic parameters. Results: The positive rate of VEGF-A was 72.3%(47/65),which was positively correlated with sentinel lympha nodes metastasis(r=0.453,P

0.05).Conclusion:QDs show no effect on cytobiological behavior of living MG-63 cells.

This study was designed to assess the immune protective effects of the vaccine strain of a precocious line of Eimeria necatrix with different doses and at different immunization times. The immunizations had a negative effect on weight gains of chickens to a certain degree but could be compensated during the “compensatory growth period” after immunity was established in the chickens. The number of oocysts excreted was positively correlated with the immunization dose. All the immunized chickens, whether they were immunized once or twice or immunized with different doses of sporulated oocysts, were able to resist attack from 1x105 virulent sporulated oocysts of E. necatrix. The lesion scoring showed that no significant difference existed in the chicken groups immunized with different doses (300 and 600) of sporulated oocysts. However, a difference existed in the immune homogeneity established in the different immunized groups, and two artificial immunizations were superior to one artificial immunization, indicating that two could extend the duration of oocyst excretion and allow more chances for the immunized chickens to become repeatedly infected.

Objective To investigate the relationship of change in hemodynamics of portal system and liver reserve of Chile-Pugh classification in patients with hepatocirrhosis.Methods Color Doppler ultrasound were applied to detect the inner diameter,average velocity of blood flow,quantity of blood flow of portal vein and splenic vein for 173 patients with post-hepatitis hepatocirrhosis(61 of Child grade A,53 of grade B,59 of grade C),which were sub- sequently compared with the healthy controls.Results In patients with hepatocirrhosis,the inner diameter of portal vein and splenic vein were widened,the average velocity of blood flow of portal vein and splenic vein were slowed down and the quantity of blood flow of splenic vein was increased,and the worse the damage of liver function was, the more obvious the change became.Conclusion The change in hemodynamics of portal system varies with the damage in liver function in patients with hepatocirrhosis.Measuring the hemodynamics of portal system is significant in determining the severity of the disease and the prognosis.

Adult ; Anaphylaxis ; etiology ; Female ; Humans ; Male ; Semen ; immunology

Microorganism distributes in the organs of human body which connect with external environment, especially those organs in the gastrointestinal tracts, and it also plays a fundamental role in the physiopathology of the host's body. Because the microorganism is very small and has a great variety, it is difficult to reveal the significance of microorganism in the human physiopathology comprehensively and deeply. With the development of molecular biology, genomics, bioinformatics and other disciplines, the microbiome research will be more possible and easier. There are two key contents of microecology. One of these is to identify and quantify the diversity of microorganism, and the other is to reveal activity and the physiopathological function of microorganism in the host. Microbiome research methods, therefore, can be summarized as the traditional detection methods, construction of gene library, the genetic fingerprint analysis and molecular hybridization techniques and so on.
Computational Biology ; Gastrointestinal Microbiome ; Gastrointestinal Tract ; microbiology ; Humans

BACKGROUND:After cerebral ischemia/reperfusion injury,as a executioner caspase, procaspase-3 and caspase-3-like activity increased significantly. We observe both the dephosphorylated and phosphorylated procaspase-3, and try to find out their variations during the processes of cerebral ischemia/reperfusion injury.OBJECTIVE:Observe the expression of procaspase-3 in hippocampus following transient forebrain ischemia.DESIGN: A randomized and controlled experiment.DEPARTMENT:Department of Biochemistry and Molecular Biology,Capital University of Medical Sciences.SETTING:Department of Hyperbaric Oxygen of Beijing Chaoyang Hospital Affiliated to Capital University of Medical Sciences.METHODS:Transient forebrain ischemia was induced by bilateral common carotid occlusion (BCCAO) for 20 minutes. Hippocampus was obtained at reperfusion time points of 6 hours, 12 hours,24 hours and 48 hours respec-tively after 20 minutes of BCCAO. Sham-operated group did not occlude bi-lateral common carotid, and hippocampus was obtained at reperfusion time point of 24 hours. Western Blotting was used to detect the level of procaspase-3.MAIN OUTCOME MEASURES:The comparison of total procaspase-3,dephosphorylated procaspase-3 and phosphorylated procaspase-3 level in hippocampus between each group.3 level: Total procaspase-3 level increased in hippocampus at reperfusion time points of 12 hours and 24 hours post-BCCAO (9 133.1 ±2 216.3,dephosphorylated procaspase-3 level increased in hippocampus at reperfusion time point of 24 hours post-BCCAO (7812.0±1625.1, 3825.8±155.6, P was not significant (P ＞ 0.05) as compared with the expression levels in sham-operated mice.CONCLUSION: Procaspase-3 is upregulated after ischemia/reperfusion.The increment of dephosphorylated form of procaspase-3 was higher than that of phosphorylated form of procaspase-3 upon cerebral ischemia/reperfusion injury, which indicates that cerebral ischemia/reperfusion injury possibly induced the dephosphorylation of procaspase-3 and promoted its transforming into activated form.

OBJECTIVE: Superoxide dismutase is one of the families of enzymes which is ubiquitous, and efficiently catalyzes the dismutation of superoxide anions.There are three superoxide dismutases in human brain that are of benefit to ischemic damage. In recent years, transgenic and knockout mutant mice has been widely used in the researches of superoxide dismutases for its protective effect in cerebral ischemia.DATA SOURCES: Using the terms "Chan PH and superoxide dismutase and cerebral ischemia", the relative articles published in the English from January 1991 to December 2003, were identified by searching the MEDLINE database with computer.STUDY SELECTION: The trials with transgenic and knockout mutant mice were identified preliminarily, then all remainders were searched in full content and were divided into intervention group and controlled group, the random cases were accepted, non-random excluded.DATA EXTRACTION: A total of 38 fully studies related to cerebral ischemia and superoxide dismutase were collected, among them, 13 random cases were accepted, and 25 excluded which included 15 repeated studies and 10 irrelevant studies.DATA SYNTHESIS: Reactive oxygen species involved in redox signaling pathways in ischemic cerebral, it can also cause oxidative damage of some macromolecules cell such as lipids, proteins and neucleic acid. In the ischemic brain tissue, overproduced oxygen radicals and consumption of superoxide dismutases lead to tissue damage. Copper-zinc superoxide dismutase, manganese superoxide dismutase and extracellular superoxide dismutase have the same function which can decompose superoxide anion radicals to form H2O2 that are scavenged further, thereby ameliorate edema formation,infarction and cell death, improve the function of ischemic cerebral tissue further.CONCLUSION: Increased oxygen free radicals is an important mechanism in ischemic cerebral damage, all three SOD can ameliorate cerebral ischemia. The protect effect of three superoxide dismutase is related to DNA repairing enzyme, transcription factor, apoptosis proteins and signal transduction pathway.

It has been demonstrated that certain members of cyclophilins (CyP) family are related to tomurigenesis, which might result from the role for Cyp in cell proliferation. Most members of Cyp family express specifically in tumor tissue in contrast to in normaltissue and cancer-brink tissue. It is possible that a novel approach to cancer therapy and diagnosis would be appeared through exploring the relationship between Cyp and tumor.

The experiments were designed to study the relation between endotoxamia and renal insufficiency and the protective effects of RSM on galactosamine-induced acute hepatic failure. The results indicated that galactosamine-treated rats which all accompanied with endotoxamia exhibited significant renal dysfunction (Per, BUN elevated; UV, Ccr, U_(Na)V fell; FE_(H2O), FE_(Na) enhanced). After treatment of RSM, the endotoxemia was reduced while renal functions were improved. It suggested that there was a close relationship between endotoxemia and renal insufficiency. The experiment proved that the endotoxin was an important factor in the occurance and development of HRS. RSM possessed marked preventive and theraputic effects for HRS. In addition, the model of galactosamine-induced hepatic failure could be used to study the pathogenesis, prevention and therapy of HRS simultaneously.