Recently,a large family of G-protein coupled receptors (GPCRs) has been identified as mas-related genes (Mrgs),Which are specific expressed in small diameter sensory neurons in the trigeminal and dorsal root ganglia,suggesting a role in pain transmission.Mrgs receptors have been shown to modulate some physiological and pathological activities,such as pain and immunity.It is important to discover ligands of Mrgs for understanding and elucidating their potential physiological and pathophysiological roles.The studies on pharmacological spectrum of NPS make it an interesting target for pharmaceutical development.

All previous literatures about Chinese herbal medicines show distinctive traditional Chinese medicine (TCM) flavors. Compendium of Materia Medica is an influential book in TCM history. The TCM flavor theory and flavor standardization principle in this book has important significance for modern TCM flavor standardization. Compendium of Materia Medica pays attention to the flavor theory, explain the relations between the flavor of medicine and its therapeutic effects by means of Neo-Confucianism of the Song and Ming Dynasties. However,the book has not reflected and further developed the systemic theory, which originated in the Jin and Yuan dynasty. In Compendium of Materia Medica , flavor are standardized just by tasting medicines, instead of deducing flavors. Therefore, medicine tasting should be adopted as the major method to standardize the flavor of medicine.
Materia Medica ; standards ; Medicine, Chinese Traditional ; Reference Standards ; Taste

Objective To explore clinical significance of subtemporal decompression in traumatic total anterior circulation infarcts(TTACI).Methods 24 TTACI cases accepted subtemporal decompression were analyzed including CT scan feature, pre operative observation to temporal superficial artery, operative cranial pressure classification and blood circulation in cortical small artery.Results Clinical symptom compared between before and after operation: good 4 cases, unchanged 14 cases, dead 6 cases. CT scan showed infarct areas became smaller in 4 cases, unchanged in 9 cases, larger in 2 cases three months after operation.Conclusion Subtemporal decompression cannot reduce the areas of infarcts and mortality, nor improve neurological function and promote quality of survivor.

Once uveal melanoma (UM) has distant metastasis, the median survival time of the patient is less than 12 months. There is currently a lack of standard treatment for metastatic UM. In recent years, immunotherapy is splendid in the field of oncology. Immune checkpoint therapy, cancer vaccine therapy and T cell adoptive therapy have been applied to UM therapy. However, most of the clinical effects are limited and the survival benefit is not high. The recent early research results of the new immunotherapeutic drug IMCgp100 are encouraging.

Aim To evaluate the effects of aspirin on the expression of inflammatory proteins induced by oxidized low-density lipoprotein(ox-LDL) in human vascular endothelial cells (HUVECs). Methods HUVECs were stimulated with different concentrations of ox-LDL. The expression of inflammatory proteins was detected by Western blot.Intracellular ROS generation was measured by flow cytometry using perexide-sensitive flurscent probe 2′, 7′-dichrofluorescein diacetate(DCFH-DA).Results ① Aspirin inhibited COX-2 expression induced by ox-LDL. Cells were preincubated with 2.5 mmol?L-1, 5 mmol?L-1 of aspirin or without any treatment for 30 min and then stimulated by 0.3 g?L-1 ox-LDL for 16 h, COX-2 expression was reduced by treating of aspirin.COX-2 expression was enhanced after the stimulation with ox-LDL, and aspirin inhibited the increasing.② Aspirin suppressed ICAM-1 expression induced by ox-LDL in HUVECs. ICAM-1 expression was increased by ox-LDL stimulation for 16 h, and aspirin significantly down-regulated the expression. Similar results were obtained by immunofluorescence.③ Aspirin partially reduced ROS production induced by ox-LDL in HUVECs. After stimulation with 0.3 g?L-1 ox-LDL for 16 h, the intracellular level of ROS was increased, however, aspirin failed to fully inhibit the phenomenon.Conclusion Nonsteroidal anti-inflammatory drugs (NSAID) aspirin significantly down-regulated the expression of COX-2 and ICAM-1 induced by ox-LDL.The results suggested that aspirin could reduce the inflammation responses mediated by ox-LDL on HUVECs in atherosclerosis.

Neuronal α4β2 nicotinic acetylcholine receptors (nAChRs) are the most widespread subtypes in central nervous system, α4β2 nAChRs mainly exist in procerebrum, parietal lobe cortex, temporal lobe cortex, hippocampus, basal ganglion and cerebellum. Researches on gene knock-out mice have demonstrated that α4β2 receptors participate in the development, aging, neuron survival, pain, and learning and memory, and are also involved in gamma-aminobutyric acid release and dopaminergic function. Studies have disclosed that α4β2 nAChRs relate to the development of variety of neurological diseases, including pain, Alzheimer's disease and Parkinson's disease.

AIM: To investigate the effect and mechanism of Jingui Shenqi Pill(JGSQ) on pulmonary fibrosis. METHODS: Pulmonary fibrosis was induced by bleomycinA5 in SD rats.Rats in drug group were killed after being treated by taking JGSQ daily for 28 days.The lungs of all rats were harvested for histopathological studies.The contents of tumor necrosis factor ?(TNF-?)in lungs were measured and compared. RESULTS: The degree of the alveolitis and pulmonary fibrosis, the contents of TNF-? in lungs increased significantly in the model group as compared with the normal group and the JGSQ treated group(P

Objective To isolate and identify exosomes from human serum , explore the feasibility of detecting exosomal miRNA in human serum.Methods Retrospective study.Serum samples from 10 healthy individuals in January 2013 were randomly selected.Besides, from January 2013 to December 2014, serum samples from prostate cancer(PCa) patients (n=20), benign prostatic hyperplasia(BPH) patients ( n=20 ) and healthy controls ( n=20 ) were selected.Exosomes were isolated from these serum samples using ExoQuick , and then identified by using transmission electron microscopy , NanoSight nano particle analyzer and Western Blot for morphology and molecular phenotype.The quality of exosomal RNA was analyzed using Agilent 2100 Bioanalyser.Then quantificational real-time polymerase chain reaction ( qRT-PCR) was carried out to detect miRNAs in different components of human serum ,and nonparametric tests were used for difference analysis.Results Exosomes isolated from human serum showed round or oval vesicles, mainly in diameter 40-100 nm, and with maximum peak distribution of 58 nm.Moreover, they expressed HSP70 and four transmembrane protein CD 63.Agilent 2100 Bioanalyzer results showed that the major RNA component of exosome was about 25nt small RNA.qRT-PCR confirmed that 4 normal miRNAs were expressed in human serum exosome , and the expression of miRNAs in exosome pellets were higher than the whole serum (miR-21, U =16,P =0.007 2; miR-16, U =3,P<0.000 1; miR-20a, U =2,P <0.000 1;let-7a, U=13,P=0.003 2) and exosome-depleted supernatant ( miR-21, U=15,P=0.006 5;miR-16, U=2,P<0.000 1;miR-20a, U=1,P<0.000 1;let-7a, U=10,P=0.002 8).miR-141, the molecular marker of prostate cancer ,were analyzed by qRT-PCR in whole serum samples and serum exosome pellets isolated from the same serum in a cohort of 20 PCa patients , 20 BPH patients and 20 healthy control people.The results showed that , in three groups , exosomal miR-141 expression were all significantly higher than serum circulating miR-141 (Control group, U=66,P=0.000 3; BPH group, U=83,P=0.001 6;PCa group, U=54,P<0.000 1).In addition, the expreession of exosomal miR-141 in PCa patients was significantly higher than BPH patients or healthy controls (3.85 fold, U=74,P=0.000 7 and 4.06 fold, U=70,P=0.000 5).Conclusion Exosome can be efficiently isolated from human serum.Compared with the whole serum , isolation of serum exosome may helpful to improve the detection of circulating miRNA.

Neuronal?_4?_2 nicotinic acetylcholine receptors(nAChRs) are the most widespread subtypes in central nervous system.?_4?_2 nAChRs mainly exist in procerebrum,parietal lobe cortex,temporal lobe cortex,hippocampus,basal ganglion and cerebellum.Researches on gene knock-out mice have demonstrated that?_4?_2 receptors participate in the development,aging,neuron survival,pain,and learning and memory,and are also involved in gamma-aminobutyric acid release and dopaminergic function.Studies have disclosed that?_4?_2 nAChRs relate to the development of variety of neurological diseases,including pain,Alzheimer's disease and Parkinson's disease.

The SEM specimens of the blood vessels of the gall-bladder in the full term fetus were produced with the methyl methacrylate cast. The specimens were dryed and gilded with EIKO. IB-3. and then observed under scanning electron microscope. The microvessels of the wall of gall-bladder obviously were divided into three layers, namely: serous vessels, muscular vessels and mucous vessels. The serous and muscular vessels are similar to that of the intestinal canal. However the mucous vessels were characterized by subepithelial capillary networks and veins of large calibre in the lamina propria. The capillary networks were connected directly with the venous plexus by the capillaries. There are fewer arterioles passing and branching among the venous plexuses. Each arteriole was connected to capillary networks. The short capillary was seen frequently between the arterioles and the venous plexus, serving as communication between them.