Select 106 pregnant women with threatened preterm labor between 28 and 37 weeks of gestation. They have been treated continually by the preventing preterm labor therapy including antibiotics and magnesium sulfate. Cervicovaginal fetal fibronectin (fFN) and C-reactive protein (CRP) in serum were detected before treatment and after 3-day and 7-day treatment respectively. 100 normal pregnant women were included as control group. (1) The fFN and CRP had significant differences between study group and control group (P<0.05). (2) The fFN and CPR were different compared in the treatment period (P<0.05). Fetal fibronectin and CRP can be used can be used in therapeutic effect evaluation of threatened preterm labor.
C-Reactive Protein ; analysis ; Female ; Fibronectins ; blood ; Humans ; Infant, Newborn ; Obstetric Labor, Premature ; diagnosis ; prevention & control ; Pregnancy

Pathological changes of Kupffer cells in number and ultrastructure were studied by means of colloidal carbon uptake, endogenous peroxidase cytochemistry, quantitative histology and ultrastructural stereologic morphometry at various time intervals (3,7,14,21,and 28 d) after common bile duct ligation. Kupffer cell phagocytic function and serum fibronectin opsonic activity were measured. The results show the morphological changes of Kupffer cells coinciding with their functional alterations. Alteration of Kupffer cell phagocytic function is mainly due to the changes of number and cellular activity of Kupffer cells.

Objective To explore the relationship between serum hs -CRP,IL -17 levels and senium type 2 diabetic macrovascular complications and its clinical significance.Methods The serum levels of hs -CRP and IL-17 were measured with immunoturbidimetry and ELISA respectively in 80 cases of type 2 diabetic patients with macrovascular complications,80 cases of the absence of any major vascular complications in patients with type 2 diabetes and 80 healthy subjects as the control.Results The serum hs -CRP and IL -17 levels of senium type 2 diabetic Patients with macrovascular complications were significantly higher than those of patients without macrovascular complication and healthy control group(t =19.18,23.14,all P <0.01);And serum hs -CRP and IL -17 levels of seniumtype 2 diabetic patients without macrovascular complications were also significantly higher than those of the healthy control group (t =23.64,19.97,all P <0.05 );While serum hs -CRP and IL -17 levels in different subgroup of type 2 diabetic patients with macrovascular complications were no significantly statistical significance(F =1.08,1.49,all P >0.05);In patients of type 2 diabetic Patients with macrovascular complications,hs -CRP was positively correlated with serum IL -17(r =0.712,P <0.05).Conclusion Hs -CRP and IL -17might be involved in the process of inflammatory response to diabetic macroangiopathy,and may have important role in the pathogenesis of senium type 2 diabetic patients with macrovascular complications.Detemination of hs -CRP and IL -17 levels in type 2 diabetic patients may be useful for early detecting diabetic macrovascular complications and serve as the index in monitoring the state of diabetic macrovascular complications.

Objective: To study the lung tissue TLR4 expression and airway hyperresponsiveness(AHR) in the mice infected by respiratory syncytial virus(RSV).Methods:16 clean,6 weeks old BALB/c mice were divided into two groups equally(RSV group and control).One week after RSV infection,AHR was detected by inhaling the increased concentration of methacholine,TLR4 mRNA expression was measured by RT-PCR methods,and the correlation between AHR and TLR4 expression was also analyzed.Results:An important difference was detected in two groups' airway resistance when the inhaled concentration of methacholine was 3.2g/L(P

Objective To study the clinical values of neuron-specific enolase(NSE) as serum tumor marker in lung cancer.Methods The serum NSE levels in 96 patients with lung cancer,60 patients with begnign pulmonary disease and 60 healthy controls were measured by using enzyme-linked immunosorbent assay.Results The levels of NSE in serum in patients with lung cancer was significantly higher than those in healthy subjects and patients with begnign lung disease(P0.05).The effective rate of chemotherapy were 87.10%,40.00% and 66.67%, 33.33% in patients with SCLC and NSCLC,respective for NSE positive and negative ones(P

Objective To discuss the clinical effect of sublingual misoprostol on planned delivery with full-term pregnancy and influence on outcomes of pregnancy and newborns.Methods Ninety-six puerpera with cervical bishop score ≤ 4 were randomly divided into the control group(n =48) and research group (n =48).The patients of the control group were given oxytocin therapy while the research group were given sublingual misoprostol.The promoting effect of cervical maturity,induced abortion effects,outcomes of pregnancy and newborns between the two groups were compared.Results The total effective rate of cervical maturity of research group was obviously higher than that of control group,the differences were statistically significant (91.7％ (44/48) vs 66.7％ (32/48),x2=7.834,P =0.015).The total effective rate of induced abortion effects of research group was obviously higher than that of control group,the differences were statistically significant (93.8％ (45/48) vs 54.2％ (26/48),x2=9.653,P=0.004).The postpartum hemorrhage((183.6±24.2) ml vs.(216.5±32.6) ml,t=6.748),total stageoflabor((7.6±1.3) h vs.(11.3±2.5) h,t=6.636) and cesarean section rate(31.3％ vs.47.9％,x2 =5.637) of research group was obviously lower than that of control group,the differences were statistically significant (P＜0.05).Two groups of newborns and the incidence of neonatal asphyxia,amniotic fluid pollution had no statistically significant difference(P＞0.05).Conclusion The clinical effect of sublingual misoprostol on.planned delivery with full-term pregnancy is obvious and causes little influence on outcomes of pregnancy and newborns.It is safe and effective to be applied on planned delivery and worth popularization and application.

Objective To study the treatment of postoperative pancreatic fistula. Methods The study (retrospectively) reviewed the management of 20 patients with pancreatic fistula. Fifteen patients were treated (conservatively), and 5 patients were treated surgically. Results All of the patients treated conservatively or by (surgery) were cured,and the results were satisfactory. Conclusions The majority of pancreatic fistulas can be cured with conservative management. If the fistula communicates with the main pancreatic duct or fails to heal after 5 months of conservative therapy, operation is necessary.Pancreatic fistulojejunostomy is a simple, safe and effective method.

BACKGROUND: Intestinal mucosa was damaged by ischemia and hypoxia during severe burn, and injury and infection of oxygen-derived free radicals during reperfusion.OBJECTIVE: To study on the basis of sub-cell level whether danshen can improve respiratory function of mitochondrion of epithelial cell in intestinal mucosa and decrease the production of oxygen-derived free radicals so as to protect intestinal mucosa of burnt rats.DESIGN: Randomized controlled animal study with single sample.SETTING: Department of Burn, the Third Hospital of Chinese PLA.MATERIALS: The experiment was conducted at the Surgery Institute of the Third Hospital of Chinese PLA from December 2001 to February 2002.Totally 96 SD rats of clean grade were randomly divided into normal control group (n=24), burning model group (n=36) and danshen treatment group (n=36). 1 mL danshen parenteral solution had 1.5 g raw materials.METHODS: ① Rats in the burning model group and danshen treatment group were used to establish grade Ⅲ burning models with 20% scald of total body surface. ② Rats in the normal control group were not injured.③ After modeling, 1 mL/kg danshen parenteral solution was slowing injected into rats in the danshen treatment group through femoral vein, but saline was injected slowly into rats in the normal control group and burning model group. ④ Twelve rats from each of the burning mgdel group and the danshen treatment group were sacrificed at 1, 2 and 6 hours after modeling, and 8 rats in the normal control group were sacrificed at relevant time points respectively. ⑤ Samples of small intestine were collected to measure cytochrome aa3, cytochrome C, level of energy charge and activity of superoxide dismutase of mitochondrion of epithelial cells in intestinal mucosa.MAIN OUTCOME MEASURES: Cytochrome aa3, cytochrome C, level of energy charge and activity of superoxide dismutase of mitochondrion of epithelial cells in intestinal mucosa 1, 2 and 6 hours after modeling.RESULTS: Totally 96 SD rats entered the final analysis. ① Level of cytochrome aa3 of mitochondrion of epithelial cells in intestinal mucosa at various time points after modeling: Levels of cytochrome aa3 did not changed obviously in the burning model group and danshen treatment group 2 hours after modeling as compared with that in the normal control group (P＞ 0.05). Six hours after burning, levels in the burning model group were obviously lower than those in the normal control group (P ＜ 0.05), but those in the danshen treatment group were obviously higher than those in the burning model group [(3.16±0.13), (2.5640.15) μkat/g, P ＜ 0.05]. ②Measurements of cytochrome C, level of energy charge and activity of superoxide dismutase of mitochondrion of epithelial cells in intestinal mucosa at various time points after modeling: As compared with those in the normal control group, measurements were decreased obviously in the burning model group 1, 2 and 6 hours after modeling (P ＜ 0.05 or 0.01), but those in the danshen treatment group were obviously higher than those in the burning model group (P ＜ 0.05 or 0.01).CONCLUSION: Danshen can raise the levels of cytochrome aa3, cytochrome C, energy production and SOD, and also reduce the production of oxygen-derived free radicals so as to improve respiratory function of mitochondria of epithelial cells in intestinal mucosa of rats.

Tumor Markers(Tumor Markers,TM) as a member of the tumor microenvironment components,change with the tumor microenvironment and the functional status of the body.Chinese medicine can improve the overall regulation of tumor micro-environment impact on the TM,which to some extent,the dynamic detection of TM can be reflected in the earlier short-term medical treatment of cancer patients.Based on TM,we clarified the relationship of differentiating disease and differentiating syndrome,type of syndrome and histology pathology type for tumor.This will provide new theoretical foundation for clinical anti-tumor studies from the microcosmic scope.

Reversible posterior leukoencephalopathy syndrome (RPLS) is a cliniconeuroradiological entity mainly characterized by the rapidly progressive increase in blood pressure, headache, vomiting, conscious disturbance and seizure. Neuroimaging showed symmetrical reversible white matter edema in bilateral cerebral hemispheres, particularly in posterior brain.Clinical symptoms and neuroimaging changes can be recovered completely with timely and correct treatment. The two major hypotheses about the pathogenesis of RPLS-cerebral vasospasm and cerebrovascular hyperperfusion-have been in dispute. At preseut, most researchers still agree that the latter is the main cause of cerebral edema. This article expounds the new viewpoints of its pathogenesis and the above two major hypotheses in recent years from the characterizations of etiology, pathology and imaging of RPLS.