1.The correlation between laboratory and clinical wearing resistance of resin composites
Jianmin HAN ; Gang ZHENG ; Hong LIN ; Rui ZHENG
Journal of Practical Stomatology 1995;0(04):-
Objective: To discuss the feasibility of a new method to evaluate wearing simulators,and to investigate the feasibility of specimens' depth loss as the index of the materials' wearing resistance.Methods: Two commercial composite resins(SureFil and Spectrum TPH) were selected.The specimens were subjected to 100,200,300,400,500,800,1200,1 600 and 2 000 cycles wearing in the CW3-1 wearing system.Wearing was determined quantitatively by weighing the specimen and measuring the height of the specimen,volume loss(mm 3) and height loss(?m) were calculated.Previous clinical study results on SureFil and Spectrum TPH conducted at Hong Kong University were used to examine the relationship between clinical wearing and laboratory wearing.The difference of the wearing resistance between the two materials and the Pearson correlation of the height loss and the volume loss were analyzed by statistical software SPSS 15.0.Results: The wearing resistance of the two materials was significant difference(P
2.Study on the HPLC Fingerprint of Dry Ginger
Qi GUO ; Xiaohong ZHAO ; Haijian HAN ; Rui SHEN ; Ying YANG ; Hong LEI ; Yongshen LI
China Pharmacist 2015;(3):397-399
Objective:To establish the HPLC fingerprint of dry ginger to provide experimental evidence for the quality control. Methods:An Agilent TC-C18 column (250 mm × 4. 6 mm,5 μm) was used, the flow phase was acetonitrile-water with gradient elu-tion, the flow rate was 1. 0 ml·min-1 , the column temperature was 30℃, and the detection wavelength was 240 nm. The data were e-valuated by the similarity evaluation software for TCM fingerprint. Results:There were 10 common peaks in HPLC chromatogram of 10 batches of dry ginger at 240 nm, and the chemical similar coefficient was 99%. Conclusion: The fingerprint of ginger at 240 nm is highly specific and typical with a rich fund of information, which can provide useful references for the quality control and evaluation of dry ginger.
3.Analysis of the incidence of hospitalized children with Henoch-Sch(o)nlein purpura and Henoch-Sch(o)nlein purpura nephritis
Hong LIU ; Rui FU ; Douxing HAN ; Baojin HU ; Weimin ZHENG ; Yuhui HUANG
Chinese Journal of Rheumatology 2013;17(11):756-759
Objective To analyze the incidence of hospitalized children with Henoch-Sch(o)nle in purpura (HSP) and Henoch-Sch(o)nlein purpura nephritis (HSPN) from 2009 to 2012,and to characterize the epidemiology of HSP and HSPN in Jiangxi province.Methods Inpatients of Jiangxi Children's Hospital with the diagnosis of HSP or HSPN were recruited during 2009 to 2012.The basic messages in the home page of medical records,such as the admission year,sex,age,area coming from were collected.Chi-square test was used for statistical analysis.Results A total of 2516 HSP pediatric patients were included in this study.Of whom,412 cases were diagnosed in 2009,568 cases in 2010,750 cases in 2011,786 cases in 2012.Among them,renal damage as the presenting symptom in 110 cases in 2009,148 cases in 2010,198 cases in 2011,and 196 cases in 2012.The average incidence of HSPN was 25.91% (652/2516).The morbidity of HSPN were similar in different admission years (x2=0.62,P>0.05).In HSP patients,the ratio between male and female was 1.59∶1; the peak age was 4 to 9 year-old (65.50%,1648/2516).The morbidity of HSPN was similar in both girls and boy patients group (x2=0.14,P>0.05).The morbidity of HSPN was 18.76% (163/869) in patients younger than 6 years old,which was lower than 26.85%(359/1337) in patients between age 6 to 11 years old,and the morbidity of HSPN was 41.94% (130/310) in patients older than 11years old,the difference was significant (x2=65.24,P<0.01).The morbidity of HSP in the month of year was different,the peak time was between October and December,which as 40.74%(1025/2516).The morbidity of HSPN was 30.23%(208/688) during January to March,higher than in other period of the year (x2=9.87,P<0.05).In the last four years,there were 824(32.75%) hospitalized patients of HSP in Nanchang district,1692 cases (67.25%) in other areas in Jiangxi province.The morbidity of HSPN in Nanchang district was 17.35% (143/824),compared with the average level 25.91%(652/2516),the difference was significant (x2=25.08,P<0.01).Conclusion The number of children diagnosed with HSP is progressively increased from 2009 to 2012 in Jiangxi province.The peak period for HSP is between October and December,and the peak age is 4 to 9 year-old.The incidence of HSPN between January to March group is higher than in other periodof the year.The morbidity of HSPN is increased with age.
4.Relationship of Renal Injury and Expression of Macrophage Migration Inhibitory Factor in Renal Tissue of Henoch-Schonlein Purpura Nephritis in Children
rui, FU ; dou-xing, HAN ; yin, ZOU ; hong, LIU ; bao-jin, HU ; qiang, XIAO
Journal of Applied Clinical Pediatrics 2006;0(23):-
Objective To investigate the expression of macrophage migration inhibitory factor(MIF) in renal tissue of children with Henoch-Schonlein purpura nephritis(HSPN),and its correlation with clinical indexes and pathological changes,and to explore its effect on the pathogenesis of HSPN.Methods According to the clinical manifestation,60 children with HPSN were divided into only purpura group,mixed group and HSPN group.MIF concentration of Henoch-Schonlein purpura(HSP) groups and healthy control group were detected with enzyme linked immunosorbent assay(ELISA).MIF protein expression and the marker of human macrophage(CD68) in renal tissues of HSPN and normal control group were detected with immunohistochemistry method.The total urine protein for 24 hours and urinary N-acetyl-beta-D-glucosaminidase (NAG) level were detected with laboratory routine method.Results MIF concentration in mixed group and HSPN group were significantly higher than that in only purpura group and healthy control group(Pa
5.Prevalence of Hearing Disorders Based Whole Population in Jilin Province, China
Xiangyang HU ; Mo LONG ; Rui HAN ; Lijun ZHOU ; Fang WANG ; Rui GONG ; Hong LI ; Cuiying GUO ; Xuegang SUN ; Bo DU ; Tao PAN ; Shaoxing ZHANG ; Xiaoying ZHENG
Chinese Journal of Rehabilitation Theory and Practice 2016;22(3):330-334
Objective To investigate the prevalence, etiology, rehabilitation demands and service condition of hearing disorders based on the whole population in Jilin Province, China. Methods Using the probability proportion to size (PPS) sampling, 9246 (93.3%) out of 9909 residents sampled form 36 counties were targeted for investigation from August, 2014 to January, 2015, followed the WHO Ear and Hearing Disorders Survey Protocol. The hearing loss and disability were classified as WHO recommended and Classification and Grading Criteria of Disability (GB/T 26341-2010). Results The standardized prevalence of hearing loss and disability was 16.41%and 4.78%, re-spectively. Age, sex, residence, occupation and marriage status, education level and household income were significantly associated with hearing loss prevalence, while nationality was not. The main etiologies included non-infectious disease (47.33%), ear disease (14.17%), un-known causation (13.89%), and noise (8.59%). Among all people with hearing loss, those who accepted intervention service accounted for 11.02%. Among all people with hearing disability, those who used hearing aids accounted for 5.58%, and 0.67%used artificial cochlea. Con-clusion Demographics and socioeconomic factors are significantly associated with the prevalence of hearing loss. The main etiology con-tains non-infectious disease, ear disease and noise. Both the rate of service utilization among people with hearing loss and the rate of adopt-ing hearing aids among people with hearing disability are low. It is needed to do more in prevention and rehabilitation of hearing impairment.
6.Research progress on effect factors of secondary metabolites content in callus.
Hui-Yong FANG ; Hong ZHU ; Hai-Mai DING ; Hua-Rui HAN ; Xiao-Lan LIU ; Li-Jing HAO ; Min-Hu LI
China Journal of Chinese Materia Medica 2014;39(15):2846-2850
Secondary metabolites are the result of that plant interaction with biological and non-biological factors in the long-term evolution process, and play an important role in plant growth, development and physiology. The effective components of medicinal plant are usually the secondary metabolites in plant cells, and the synthesis of them are affected by a variety of factors, such as environmental impact. Acquirement of the secondary metabolites via callus culture has the advantage of low cost and less environmental impact. The synthesis and accumulation of medicinal plant secondary metabolites are not only controlled by light, temperature and pH, but also infected by germplasm, plant growth regulator and elicitor. This article presents a review of the influencing factors, and provides a basis for further study and development.
Light
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Plant Growth Regulators
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metabolism
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Plant Physiological Phenomena
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radiation effects
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Plants
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metabolism
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radiation effects
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Temperature
7.Spaceflight-induced variation on biological traits and effective components of Cassia obtusifolia.
Ren-jun MAO ; Zhi-hong QI ; Rui-lian HAN ; Feng-hua LIU ; Yan LIU ; Zong-suo LIANG
China Journal of Chinese Materia Medica 2015;40(13):2571-2575
The dry seeds of Cassia obtusifolia were carried by the "ShenZhou 8" satellite and sowed after landing. Based on our pri- or study on SP1, the characteristics of plants growth, physiological index and content of effective components were examined. The results showed that the QC10, QC29 strains matured 5 d earlier compared with control. The plant height, across diameter and ground diameter of QC10, QC29, QC46 strains was superior to the control at whole growth period. The branch number increased ranging from 4 to 11 and the number of pods reached 321, 313,281, respectively, which was dramatically higher than the control (246). The yield of QC10, QC29, QC46 strains increased noticeably from 31.4 to 63.2 g. The 1000-seed-weight of QC10, QC29, QC46 strains was 25.86, 25.88, 24.06 g, while the control was 23.69 g. Compared to the control, the mass fraction of chlorophyll was enhanced 1.098, 1.016, 0.297 mg. There was no significant difference in aurantio-obtusin and chrysophanol content of seeds. Through two years research, three high-yield mutant strains were obtained. This study indicates that spaceflight-induced mutants could provide new germplasm for C. obtusifolia breeding and offers the theoretical basis for further utilization of spaceflight-induced mutation to breed high-quality C. obtusifolia strains.
Cassia
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chemistry
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genetics
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growth & development
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Mutation
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Space Flight
8.Evaluation of 131I treatment efficacy and prognostication for bone metastases from differentiated thyroid cancer
Zhong-ling, QIU ; Yan-hong, XU ; Hong-jun, SONG ; Li-bo, CHEN ; Rui-sen, ZHU ; Han-kui, LU ; Quan-yong, LUO
Chinese Journal of Nuclear Medicine 2011;31(3):155-159
Objective To evaluate the efficacy of 131I treatment for bone metastases from DTC and analyze the survival rates after 131I treatment and prognostic factors. Methods One hundred and six DTC patients with bone metastases treated by 131I during January 1991 and January 2009 were retrospectively analyzed. Treatment efficacy was assessed based on serum Tg change, bone pain palliation and changes on medical imaging. Univariate analysis was performed for defining the factors affecting 131I treatment efficacy. Survival curves were estimated using the life table method. Survival analysis was performed using Kaplan-Meier method. Results Serum Tg decreased dramatically in 37/106 (34.9%) patients treated with131I. Thirty-nine of 61 patients (63.9%) with bone pain had pain relief. Age, tumor subtype and presence of non-osseous distant metastases were significant factors affecting 131I treatment efficacy based on serum Tg change (χ2=6.443, 11.455, 6.756, all P<0.05), but had no significant effects on bone pain palliation (χ2=0, 0, 0.060, all P>0.05). There were no imaging changes of bone metastases in 77.4% of patients after 131I treatment. The overall 5-year and 10-year survival rates from initial diagnosis of bone metastases was 86.47% and 57.90%, respectively. Univariate analysis showed that number of metastases, presence of non-osseous distant metastases and pre-131I treatment surgery were significant factors for survival (Log-rank values were 4.05, 5.98, 4.22, all P<0.05). Conclusions 131I treatment for bone metastases from DTC is effective for lowering serum Tg and palliation of bone pain. Single metastasis, absence of non-osseous distant metastases and pre-131I therapy surgery are favorable predictors of prognosis.
9.Simultaneous determination of four constituents in Yangxin Dingji Capsules by HPLC
Han-Xue WANG ; Lin-Feng LI ; Rui AN ; Xue-Mei CHENG ; Chang-Hong WANG ; Xin-Hong WANG
Chinese Traditional Patent Medicine 2017;39(1):98-101
AIM To establish an HPLC method for the simultaneous content determination of four constituents in Yangxin Dingji Capsules (a cardiac tonic for palpitation,containing Glycyrrhizae Radix et Rhizoma Praeparata cum Melle,Cinnamomi Ramulus,Rehmanniae Radix,etc.).METHODS The analysis of 50% methanol extract of Yangxin Dingji Capsules was performed on a 40 ℃ thermostatic Diamonsil C1s column (250 mm × 4.6 mm,5 μm),with the mobile phase comprising of 0.1% formic acid-acetonitrile flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 265 nm.RESULTS Liquiritin,glycyrrhizic acid,cinnamic acid and cinnamic aldehyde showed good linear relationships within the ranges of 1.00-80.24 μg/mL (r=0.999 0),2.52-100.70 μg/mL (r--0.999 7),0.50-40.40 μg/mL (r =1.000 0) and 0.66-52.96 μg/mL (r =1.000 0),whose average recoveries were 97.74%,100.97%,101.48% and 99.49% with the RSDs of 0.45%,1.11%,1.27% and 1.66%,respectively.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Yangxin Dingji Capsules.
10.Rapid Identification of Oligosaccharide Isomers by Electron Capture Dissociation in Combination with Metal Ions
Jie Xue BAI ; Jing Wen YU ; Han Yi WANG ; Lei SHI ; Rui SU ; Feng Rui XU ; Zhou Da CHEN ; Mei Hong YANG ; Ying Shu LIU
Chinese Journal of Analytical Chemistry 2018;46(1):55-60
Oligosaccharide isomers were distinguished by electron capture dissociation Fourier transform ion cyclotron resonance mass spectrometry ( ECD-FT-ICR-MS ) in combination with utiliZing alkali, alkaline earth, and transition metals ( Na+, Ca2+, Ba2+, Mg2+, Mn2+ and Co2+) as charge carriers in electrospray.Maltoheptaose, mannohexaose and laminarihexaose were taken as examples to investigate influence of metal ions on the extent of oligosaccharide fragmentation.The same types of fragmentation ions ( 0,2 A and 2,4 A) were obtained for barium- and calcium-adducted maltoheptaose.Mg2+ and Mn2+ had the similar influence ( 0,2 A, 2,4 A and 2,5 A ).Three cross-ring cleavage ions ( 1,4 A, 2,4 A and 2,5 A ) were generated in the spectrum of cobalt-associated maltoheptaose.But in the case of doping Na+into maltoheptaose, only 0,2 A ion was detected.It was found that the signals in the spectra of mannohexaose and laminarihexaose were worse than that in the spectrum of maltoheptaose, probably resulting from different numbers of adducted metal ions.The isomers, mannohexaose and laminarihexaose could be distinguished by ECD-MS in conjunction with the addition of Ca2+, Mg2+ or Co2+.The addition of Ca2+ was the best choice for analysis of oligosaccharides.