1.Short Latency Somatosensory Evoked Potentials in Evaluation of Central Nervous System Function of Patients with Coronary Cardiac Diease
Rui-zhen TIAN ; Yuan-yuan HAO ; De-shan LIU
Chinese Journal of Rehabilitation Theory and Practice 2006;12(6):471-472
ObjectiveTo evaluate central nervous system function of patients with coronary cardiac diease by short latency somatosensory evoked potentials (SSEP).MethodsThe cerebral and spinal somatosensory evoked potentials were recorded by stimulating median nerve in 43 patients with coronary cardiac disease but without apparent nervous symptoms and 14 healthy control subjects.ResultsThe lactency periods and central conductive time of N13, N20 and P25 wave were significantly prolonged in patients with myocardial infarction (MI) or angina pectoris (AP) when compared with normal controls (P<0.05~0.001). The lactency periods and central conductive time of N20 and P25 wave recorded in MI patients were longer than those recorded in AP patients (P<0.01~0.001).ConclusionThe subclinical nervous damages in the central somatosensory pathway from spinal cord to cerebral cortex is present in patients with coronary cardiac disease especially myocardial infarction.
2.Case Report of Peliosis Hepatic in Child and Its Literature Review
zhao-yuan, QIN ; shu-mei, CHEN ; rui-de, HU ; xiao-yun, JIANG ; mei-na, LIU ; zhen-yu, SHEN
Journal of Applied Clinical Pediatrics 2006;0(19):-
Objective To report a peliosis hepatic in child and review literature and discuss.Methods Case history was inquired.Physical,labtoratory,imagement and histopathology of liver biopsy(HE staining) were examed.Results A 4-year old girl appeared dermatitis with erythema and herpes at local skin where was bit by insect before onset.The girl appeared fever,cough,then abdominal pain,hepatomegaly,pleural effusion and ascites.Lab examination revealed slight elevation of aspartate transaminase,?-glutamyltranspeptidase and alkaline phosphatase.The liver B-mode ultrasonography and CT scan revealed hepatomegaly with density heterogeneity of the parenchyma.The liver biopsy revealed many small capsule filled with blood cells.Conclusions Clinical characteristics of the disease are fever,upper abdomen pain,janundice,ascites and hepatomegaly.The diagnosis shall be combined with the pathologic biopsy of liver.
3.Therapeutic effects of left ventricular assist device on pump failure caused by acute myocardial infarction.
Dong-ya WANG ; Xiao-ying LI ; De-xin LIU ; Rui-han CHEN
Chinese Journal of Applied Physiology 2004;20(2):130-134
AIMTo evaluate the therapeutic effects of a left ventricular assist device (LVAD) on pump failure caused by acute myocardial infarction (AMI) in dogs.
METHODSThe pump failure caused by AMI was established in 18 dogs, 9 of them were treated with a LVAD that could expel the autoblood from the left ventricle into the aorta and named the experimental group, and the rest of them were treated with intravenous infusion and served as the control group. The changes of arrhythmia, mortality, pulmonary capillary wedge pressure (PCWP), left ventricular end-diastolic pressure (LVEDP), peripheral artery pressure and the diameter of left ventricular chamber were observed.
RESULTSThe ratio of ventricular extrasystole and the mortality resulted from ventricular fibrillation of the experimental group were lower than that of the control group. The systolic blood pressure of peripheral artery of the control group was significantly lower (< 100 mmHg) than that of the experimental group (>100 mmHg, P < 0.01). The PCWP and LVEDP of the experimental group during all the stages 45 minutes after the procedures were significantly lower than that of the control group (P < 0.01). The left ventricular end-diastolic diameter of the control group was larger than that of the experimental groups (P < 0.01).
CONCLUSIONTo assist circulation by expelling autoblood from left ventricle into aorta in dogs with AMI could reduce the frequency of ventricular fibrillation, improve hemodynamics, and prevent the enlargement of left ventricle. Therefore, it could play an important role in assisting the left ventricular functions.
Animals ; Assisted Circulation ; methods ; Disease Models, Animal ; Dogs ; Heart-Assist Devices ; Hemodynamics ; Myocardial Infarction ; physiopathology ; therapy ; Ventricular Function, Left
4.Prokaryotic expression and purification of mitochondrial transcription complex proteins.
Guang LIU ; Rui-Feng YANG ; Bing-Yang SHI ; De-Pei LIU
Acta Academiae Medicinae Sinicae 2011;33(6):638-643
OBJECTIVETo obtain human mitochondrial transcription factor A (TFAM), mitochondrial transcription factor B1 (TFB1M), and mitochondrial transcription factor B2 (TFB2M) that were expressed efficiently in E. coli BE21 and to purify the target proteins.
METHODSTFAM, TFB1M, and TFB2M segments were designed and synthesized. After having been sequenced, the reconstructed expression vectors were constructed by enzyme digestion and by cloning into an expression vector pET42a. Then the reconstructed vectors were transformed into E. coli BL21. Recombinant glutathione S transferase (GST) fusion proteins were expressed via the induction of IsoPropyl beta-D-ThioGalactoside (IPTG) and purified by glutathione Sepharose 4B.
RESULTSThe expression plasmids of pET42a-TFAM, pET42a-TFB1M, and pET42a-TFB1M were successfully constructed. The sequences of the cloned gene segments were identical with GenBank reported. The protein bands with relative molecular masses of 56 000, 67 000, and 69 000 appeared on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) after the expressed GST-TFAM, GST-TFB1M, and GST-TFB2M fusion proteins were separated by SDS-PAGE. The expressed fusion proteins were purified to high purity.
CONCLUSIONThe recombinant plasmids pET42a-TFAM, pET42a-TFB1M, and pET42a-TFB2M were successfully constructed, and the GST-fused target proteins were prepared.
Cloning, Molecular ; DNA-Binding Proteins ; genetics ; Escherichia coli ; genetics ; Genetic Vectors ; Humans ; Methyltransferases ; genetics ; Mitochondrial Proteins ; genetics ; Recombinant Fusion Proteins ; genetics ; Transcription Factors ; genetics
5.Replacement of artificial tracheal prosthesis by two-stage procedure with memory-alloy mesh.
Feng-rui ZHAO ; Yin-he ZHANG ; De-ruo LIU ; Shi-min LIU ; Jian-jun YU ; Yu-ling XIN
Chinese Journal of Surgery 2003;41(3):201-204
OBJECTIVETo design an artificial trachea which can totally heal with the native trachea.
METHODSUsing memory-alloy mesh as the skeleton to construct an artificial trachea by two-stage operation. After 2-year animal experiment we successfully performed the operation in a patient with recurrent carcinoid of the trachea, radically resected the tumor and primarily reconstructed the trachea.
RESULTSThe inner side of this "sandwich" artificial tracheal prosthesis was coated with skin and outside the memory-alloy mesh was muscle and vessel pedicle with good blood supply. The upper and lower anastomosis completely healed with recipient's trachea with a full recovery of trachea. Six-month follow-up showed that the patients resumed their normal life.
CONCLUSIONThe artificial trachea completely healed with the native trachea and became a part of the human trachea. The inner side of artificial trachea is coated with intact native skin tissue with ample blood supply, totally alive without rejection. Therefore, the pedicled artificial tracheal prosthesis is an real artificial trachea.
Alloys ; Female ; Humans ; Middle Aged ; Prostheses and Implants ; Trachea ; surgery ; Tracheal Neoplasms ; surgery
6.Treatment of extremity infections with soft tissue defects with the ramified musculocutaneous flap pedicled with the descending branch of lateral circumflex femoral artery
Hui-Ren LIU ; De-Qun LIU ; Rui-Guo LI ; Lei CAO ; Xin-Zhong SHAO ; Yan-Mao ZHANG ; Li-Yuan XIANG ; Guo-Qiang WANG ; Lai-Qing SUN ;
Chinese Journal of Microsurgery 2000;0(04):-
Objective To discuss the clinical effect of the ramified musculocutaneous flap pedicled with the descending branch of lateral circumflex femoral artery treatment of bones and arthrsis of extremity in- fections with soft tissue defects.Methods The muscle flap blooded with the muscular branch of lateral fem- oral muscle and musculocutaneous flap blooded with the musculocutaneous branch were designed,all of which were pedicled with the descending branch of lateral circumflex femoral arteryIn clinic24 cases of bones and arthrosis of extremity infections with soft tissue defects were treated with this kind of ramified musculocutaneous flap.Results Of the 24 cases23 cases were survived while 1 case was lost16 cases were healed at stageⅠ8 cases were healed at stageⅡSinus has formated in 3 casesone of which twicebut they were healed in one year with the treatment of debridmentsFour cases with osteomvelitis and bone defect were treated with bone grafting in the later 6~8 months after the wound has healedTwenty-two cases were followed-up for 6~20 monthsinfcetiou didn't recur.Conclusion This kind of ramified musculocutaneous flap has such ad- vantages as longer blood vessel pediclefilling the defects completely flexible application and stronger anti-in- fectionthat it may be an effective way in treating bones and arthrosises of extremity infections with soft tissue defects.
7.Clinical value of acoustic radiation force impulse imaging for quantitative evaluation of degree of liver fibrosis in chronic hepatitis C patients.
Da-kun ZHANG ; Min CHEN ; Yang LIU ; Rui-fang WANG ; Xiao-yu DONG ; Zhi-yan LI ; Guang-de ZHOU
Chinese Journal of Hepatology 2013;21(8):599-603
OBJECTIVETo investigate the diagnostic value of acoustic radiation force impulse (ARFI) imaging technology for the assessment of liver fibrosis in chronic hepatitis C (CHC) patients.
METHODSOne-hundred-and-eight CHC patients were examined by real-time ultrasound elastography using the Acuson S2000 ARFI instrument (Siemens Healthcare) and underwent liver biopsy for pathohistological analysis. The correlation between liver fibrosis grades determined by the two approaches was analyzed. The cut-off values for diagnosis by ARFI (S more than 2, S more than 3 and S = 4) were determined by generating a receiver operating characteristic (ROC) curve.
RESULTSThe spectrum of liver stiffness detected by ARFI sonoelastography included S1 at (1.26+/-0.27) m/s (n = 36), S2 at (1.45+/-0.51) m/s (n = 31), S3 at (2.01+/-0.54) m/s (n = 27), and S4 at (2.28+/-0.82) m/s (n = 14). The ARFI values were significantly different among the four different stages of liver fibrosis (P less than 0.001). The liver stiffness detected by ARFI sonoelastography was significantly correlated with the liver fibrosis stage determined by the gold standard pathohistological analysis (Spearman's rank coefficient: 0.61, P less than 0.001). Using the ARFI technology for assessment of liver fibrosis gave areas under the ROC curve of 0.779 for S more than 2 patients, of 0.863 for S more than 3 patients, and of 0.0880 for S = 4 patients.
CONCLUSIONThe real-time ultrasound elastography ARFI technology can show the elasticity modulus of liver, and its data values positively correlate with the patho-histology grade of liver fibrosis in CHC patients. ARFI technology is easy to operate, non-invasive, and quantitative, and has potential clinical value for assessing liver fibrosis in CHC.
Adolescent ; Adult ; Aged ; Elasticity Imaging Techniques ; Female ; Hepatitis C, Chronic ; complications ; diagnostic imaging ; Humans ; Liver Cirrhosis ; diagnostic imaging ; etiology ; Male ; Middle Aged ; Young Adult
8.Experiment study of PHI on histone methylation and acetylation in Molt-4 cells.
Yi-Qun HUANG ; Xu-Dong MA ; Rui-Ji ZHEN ; Jen-Wei CHIAO ; De-Long LIU
Chinese Journal of Hematology 2007;28(9):612-615
OBJECTIVETo investigate the effect of phenyl-hexyl isothiocyanate (PHI) on acetylation and methylation of histone in acute lymphoblastic leukemia cell line Molt4.
METHODSThe inhibition of cell proliferation was observed by MTT method and clone suppression test. Apoptosis and cell cycle arrest were measured by flow cytometry. The alterations in histone acetyltransferase and acetylation and methylation of histones were detected by Western blot.
RESULTSPHI could up-regulate the expression of acetyltransferase (P300/CBP), markedly induced the accumulation of acetylated histone H3, H4 and methylated histone H3 lysine 4 (H3K4), and inhibited methylation on lysine 9 of H3 (H3K9). The epigenetic regulation resulted in cell cycle arrest at G0/G1 phase, and induction of apoptosis.
CONCLUSIONSPHI can modulate both histone methylation and acetylation. It may serve as a histone deacetylase inhibitor, and might be a potential novel anti-leukemia agent.
Acetylation ; drug effects ; Apoptosis ; drug effects ; Cell Cycle ; drug effects ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Epigenesis, Genetic ; Histone Deacetylases ; metabolism ; Histones ; metabolism ; Humans ; Isothiocyanates ; pharmacology ; Methylation ; drug effects
9.Relations between fasting serum lipids and high-sensitivity C-reactive protein level in Chengdu residents.
Yan SHU ; Sen HE ; Xiao-ping CHEN ; Zheng-bing LÜ ; Rui YANG ; Kai LIU ; Kai-jun CUI ; De-jia HUANG
Chinese Journal of Cardiology 2012;40(2):125-130
OBJECTIVETo explore the associations between fasting serum lipids and high-sensitivity C-reactive protein (hsCRP).
METHODSSerum triglyceride (TG), high-density lipoprotein cholesterol (HDL-C) and hsCRP were measured in residents of Chengdu, China. Subjects with potential factors which might influence lipids and hsCRP were excluded, 580 subjects [mean age (62.3 ± 6.6) years; male: 58.7%] were finally recruited by random sampling methods.
RESULTSThere was a weak positive relationship between TG and hsCRP (r = 0.108, P = 0.01) and a weak negative relationship between HDL-C and hsCRP (r = -0.197, P < 0.001), this was also true in the sub-group with BMI < 24 kg/m(2) (r = 0.236, -0.140 respectively, all P < 0.001). In subjects with BMI < 24 kg/m(2), the hsCRP concentration was significantly higher in subjects with higher TG or lower HDL-C (all P < 0.05). hsCRP increased in proportion with the degree of dyslipidemia. After adjusting for gender, age, TC, LDL-C, fasting blood glucose, systolic blood pressure, diastolic blood pressure, history of hypertension and diabetes, smoking and alcohol drinking, logistic regression analysis showed that the odds ratio for increased hsCRP was 1.970 in subjects with either increased TG or lower HDL-C (P = 0.105) and 9.098 in subjects with both higher TG or lower HDL-C levels (P = 0.031). However, the observed relationship between TG, HDL-C and hsCRP in subjects with BMI < 24 kg/m(2) could not be observed in subjects with subjects with BMI > 24 kg/m(2) despite significant more cardiovascular risk factors in these subjects.
CONCLUSIONSA weak positive correlation between TG and hsCRP as well as a weak negative correlation between HDL-C and hsCRP was evidenced in the whole cohort suggesting dyslipidemia might be related to enhanced inflammatory status. However, this relationship is not observed in subjects with BMI > 24 kg/m(2) despite existence of more cardiovascular risk factors in these subjects.
Aged ; Alcohol Drinking ; C-Reactive Protein ; analysis ; Cardiovascular Diseases ; blood ; epidemiology ; China ; epidemiology ; Cholesterol, HDL ; blood ; Cholesterol, LDL ; blood ; Dyslipidemias ; epidemiology ; Female ; Humans ; Inflammation ; Male ; Middle Aged ; Smoking ; Triglycerides ; blood
10.Study on negative regulatory factors in bone marrow mononuclear cells of myelodysplastic syndromes.
Hong-Ming HUANG ; Rui-Rong XU ; Run-Sheng DING ; De-Yan LU ; Hong LIU
Journal of Experimental Hematology 2004;12(5):610-614
To explore the difference of negative regulatory factors among T lymphocyte subsets in bone marrow (BM) of myelodysplastic syndromes (MDS) and their relations to apoptotic gene Fas, different lymphocyte subsets in BM were categorized by monoclonal antibodies with 3 color fluorescence using flow cytometry, and the intracellular cytokines such as tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma) were determined following marrow cells culture. Then Fas mRNA of bone marrow mononuclear cells (BMMNC) were examined by RT-PCR. The results showed that TNF-alpha, IFN-gamma levels in BM of MDS both increased, the former produced by cells CD4+CD45RO+, CD8+CD45RO+, the latter by cells CD4+CD45RO+, CD8+CD45RO+, CD8+CD45RA+, in which the cells CD8+CD45RO+ were dominant. Fas mRNA expression had relationship with IFN-gamma produced by T cells but not with TNF-alpha. It is concluded that in hematopoietic microenvironment of MDS, not only the T lymphocyte subsets are in disorder, but also negative regulatory factors secreted by T lymphocyte increase. T lymphocytes play an important role in producing IFN-gamma in patients with MDS.
Adult
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Aged
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Bone Marrow Cells
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metabolism
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Female
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Humans
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Interferon-gamma
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biosynthesis
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Male
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Middle Aged
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Myelodysplastic Syndromes
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metabolism
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pathology
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RNA, Messenger
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analysis
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T-Lymphocyte Subsets
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metabolism
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Tumor Necrosis Factor-alpha
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biosynthesis
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fas Receptor
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genetics