AIM To study comparision of water-soluble components in Danshen from different sources. METHODS To be determined by HPLC, the eluent consisted of methanol (1 % acetic acid)-water (1 % acetic acid). A gradient method was set up. The detection wavelength was set at 280 nm. RESULTS The average recovery of four components were all above 97 % , RSD was no more than 2.0 % . The calibration curve was linear. CONCLUSION The method is accurate and reliable with good reproducibility.

Objective To evaluate a new prenatal diagnosis model of chromosomal abnormalities and nine microdeletion syndromes by using both traditional karyotyping and a newly-developed rapid prenatal diagnosis technology, BACs-on-Beads (BoBs) technique. Methods From June 2012 to December 2014, 807 pregnant women with high risk after screening or with other indicators, were performed amniocentesis. Traditional karyotyping and BoBs were employed simultaneously for prenatal diagnosis. Results Thirty-two cases with chromosome aneupoidies were successfully detected both by BoBs and karyotyping, including 18 cases of trisomy 21, 6 cases of trisomy 18, 1 case of trisomy 13, and 7 cases with sex chromosome abnormality. All 8 fetuses with chromosome structural abnormalities detected by karyotyping were missed by BoBs;while BoBs contributed more in detection of five microdeletion syndrome cases, including 3 cases of DiGeorge syndromes (two with microduplication and one with microdeletion), one case of Miller-Dieker syndrome, and one case of Wolf-Hirschhorn syndrome. Conclusion Combined use of traditional karyotyping and BoBs, is a rapid and effective prenatal diagnosis model that may enlarge our horizon on chromosomal diseases and should be widely used in future clinical service.

Objective To evaluate the efficacy of combined therapies of ablative fractional Er ∶YAG laser (2,940 nm) and ALA-PDT on refractory verruca plana.Methods 120 cases of refractory verruca plana patients were randomly divided into two groups:60 cases in the control group and 60 cases in the experimental group.The control group used 10％ ALA-PDT with LED irradiation of a power density 70 of mW/cm2 at a distance of 20 cm,which lasted for 20 min each time.The experimental group was treated with ablative fractional Er ∶ YAG laser (2,940 nm) first with the fluence of 500P/cm2 and short pulse duration,and then treated 10％ ALA-PDT as mentioned before.Every patient was treated twice at two-week intervals.Three independent investigators evaluated subject outcomes at 3 months post-treatment including efficacy and side-effects.Results The effective rate of the experimental group was 86.44 ％ at 3 months post-treatment after one to two times.Meanwhile,the control group was 59.65 ％.The clinical outcome of experimental group was better than the control group.There was significant difference between the two groups (P＜0.05).The recurrent rate of experimental group was 3.39％ and 12.30％ in control group.There was significant difference between the two groups (P＜0.05).There were no obvious side-effects in both groups.Conclusions Ablative fractional laser with low fluence promotes the transdermal absorption of ALA and enhances the efficacy of PDT.

Objective To study the pharmacokinetics of oral administra-tion of moxifloxacin in Chinese healthy volunteers .Methods The study was designed as an open , randomized , parallel and single -dose cycle study.The volunteers were administered with a single dose of moxifloxa-cin 400 mg for consistent four days , respectively.The plasma concentra-tions of moxifloxacin were determined by LC -MS/MS method. The pharmacokinetic parameters of moxifloxacin were calculated by WinNon-lin 5.2.1 software.Results Six subjects quit from the study , and the main pharmacokinetic parameters of the remaining 42 subjects were as follows:t1/2 was (11.71 ±3.53 ) h, Cmax was (0.51 ±0.19 ) ×10 -4 ng? mL-1, tmax was(2.94 ±1.41) h, MRT0-24 hwas(18.02 ±4.45)h, CLz/F was ( 0.84 ± 0.75 ) × 10 -4 mL ? h-1 , AUC0-24 h was (5.82 ±1.82 ) ×10 -4 ng? mL-1? h.Conclusion The characteristics of oral moxifloxacin 400 mg has shown a high blood concentration , long half-life time and it can be well tolerated.The doses adopted in foreign study is recommended .There was a gender difference in volunteers when with same dose of moxifloxacin .

Objective:To evaluate the efficacy and safety of water injection therapy combined with medical moisturizing repair cream in the treatment of rosacea.Methods:Total of 100 patients with types I and II rosacea in the Department of Dermatology, the First Affiliated Hospital of Army Medical University were randomly divided into three groups: comprehensive group (treatment group), hydrate group (control group 1) and moisturizing group (control group 2). Comprehensive group and water light group were given water injection therapy, once a month, a total of 3 times of treatment; in the comprehensive group and the moisturizing group, topical medical moisturizing repair cream was used once in the morning and in the evening, for a total of 12 weeks. After unified training, the therapists evaluated the skin lesions according to the scoring criteria and recorded the adverse reactions, for a total of 12 weeks.Results:After 12 weeks of treatment, the integral difference values before and after treatment were 8.01± 2.43, 7.68±2.16 and 5.63 ±2.19 respectively in the comprehensive group, the water injection therapy group and the moisturizing group, with statistically significant differences ( P<0.05). The effective rates of the three groups were 94.38%, 89.91% and 48.12%. No serious adverse reactions were observed. Conclusions:The water injection therapy combined with medical moisturizing and repairing cream is safe and effective in the treatment of rosacea; it is more effective than the moisturizing and repairing cream.The water injection therapy combined with medical moisturizing and repairing cream has advantages but is not obvious enough.

OBJECTIVETo investigate the effect of air pollution on respiratory health in school-aged children in the main urban area of Chongqing, China.

METHODSThe main urban area of Chongqing was divided into polluted area and clean area according to the air pollution data shown on the Environmental Protection Agency Website of Chongqing between 2010 and 2015. A cluster sampling method was used to select 695 third- or fourth-grade children from 2 primary schools in the clean or polluted area as study subjects, with 313 children from the clean area and 382 children from the polluted area. Pulmonary function was examined for all children and a standard American epidemiological questionnaire (ATS-DLD-78-C) was used to investigate the prevalence of respiratory diseases and symptoms.

RESULTSCompared with the clean area, the polluted area had significantly higher concentrations of inhalable particles (PM), fine particulate matter (PM), and nitric oxide (NO) (P<0.05). The multivariate logistic regression analysis was performed after adjustment for confounding factors, and the results showed that compared with those in the clean area, the children in the polluted area had significantly higher risks of cough (OR=1.644), cough during cold (OR=1.596), expectoration during cold (OR=2.196), persistent expectoration (OR=1.802), and wheezing (OR=2.415). The boys and girls in the clean area had significantly higher forced vital capacity and forced expiratory volume in one second than those in the polluted area (P<0.05).

CONCLUSIONSAir pollution in the main urban area of Chongqing is associated with the increased prevalence of respiratory symptoms in school-aged children and has certain effect on children's pulmonary function.

Air Pollution ; adverse effects ; Child ; Female ; Forced Expiratory Volume ; Humans ; Logistic Models ; Male ; Respiratory Tract Diseases ; etiology ; Vital Capacity

Objective To estimate the mortality and years of life lost among youth aged 18-44 in Chongqing from 2011 to

OBJECTIVETo explore the effect of exposure to vehicle exhaust in pregnant mice on the reproductive function and DNA methylation in male offspring mice.

METHODSTwenty pregnant mice were randomized into control group and vehicle exhaust exposure group (n=10) and exposed to routine laboratory condition and to vehicle exhaust for 10 consecutive days (8 h per day) in a tunnel with a heavy traffic, where the concentrations of TSP, PM10, PM2.5, SO2 and NOX and the decibel of noise were measured. The offspring mice were raised till reaching maturity, and the epididymides of the male mice were collected to test the weight coefficients, DNA methylation level, and mRNA levels of Aldh7a1 and Rpe.

RESULTSThe body weight and the weight coefficients of the epididymides and testes differed significantly between the exposure group and the control group (P>0.05). The concentrations of TSP, PM2.5, PM10 and NOx and the decibel of noise were significantly higher in the traffic environment and the control environment (P<0.05). Reduced representation bisulphite sequencing (RRBS) and Gene ontology (GO) showed that 58 genes had significantly different methylation levels between the two groups, mostly relating to the process of spermatogenesis (P<0.05). Compared with the control group, Aldh7a1 and Rpe mRNA expressions in the testes were down-regulated significantly in the exposure group (P<0.05).

CONCLUSIONExposure of pregnant mice to vehicle exhaust causes damages of the reproductive function in the male offspring mice.

Animals ; Apoptosis ; drug effects ; Male ; Malondialdehyde ; blood ; Myocardial Ischemia ; blood ; pathology ; Myocardial Reperfusion Injury ; blood ; pathology ; Myocytes, Cardiac ; pathology ; Protective Agents ; pharmacology ; Rabbits ; Random Allocation ; Superoxide Dismutase ; blood ; Trimetazidine ; pharmacology

OBJECTIVETo assess the associations between schizophrenia and six functional genes: dopamine D2 receptor gene (DRD2), dopamine D4 receptor gene (DRD4), 5-hydroxytryptamine 2A receptor gene (5-HT2A), 5-HT6 receptor gene (5-HT6), catechol-O-methyltransferase gene (COMT) and dopamine transporter gene (DAT1).

METHODSWith the techniques of Amp-RFLP and Amp-FLP, association analysis was made between schizophrenia and the six genes in 67 schizophrenic patients from Chinese Han population.

RESULTS(1) Neither genotypes nor alleles of DRD2, 5-HT2A, 5-HT6 and COMT gene showed significant differences between patients and controls (P>0.05). (2) Six repeats (6R) in DRD4 gene, the allele of 480 bp and the genotype of 480/520 in DAT1 gene were found to be of significant differences between the two groups (P<0.05). (3) Only one negative association was observed between the 480 bp allele of DAT1 gene and schizophrenia (OR=0.441, 95% CI:0.202-0.963, Z=2.05, P<0.05).

CONCLUSIONThe 480 bp allele of DAT1 gene is negatively associated with schizophrenia in Chinese Han population, which stands for the dopamine hypothesis of schizophrenia.

Adult ; Alleles ; Catechol O-Methyltransferase ; genetics ; DNA ; genetics ; Dopamine Plasma Membrane Transport Proteins ; Female ; Gene Frequency ; Genetic Predisposition to Disease ; genetics ; Genotype ; Humans ; Male ; Membrane Glycoproteins ; Membrane Transport Proteins ; genetics ; Middle Aged ; Nerve Tissue Proteins ; Polymorphism, Restriction Fragment Length ; Receptor, Serotonin, 5-HT2A ; Receptors, Dopamine D2 ; genetics ; Receptors, Dopamine D4 ; Receptors, Serotonin ; genetics ; Schizophrenia ; genetics