BACKGROUND: Disturbance of mitochondrial fission and fusion (termed mitochondrial dynamics) is one of the leading causes of ischemia/reperfusion (I/R)-induced myocardial injury. Previous studies showed that mitochondrial aldehyde dehydrogenase 2 (ALDH2) conferred cardioprotective effect against myocardial I/R injury and suppressed I/R-induced excessive mitophagy in cardiomyocytes. However, whether ALDH2 participates in the regulation of mitochondrial dynamics during myocardial I/R injury remains unknown. METHODS: In the present study, we investigated the effect of ALDH2 on mitochondrial dynamics and the underlying mechanisms using the H9c2 cells exposed to hypoxia/reoxygenation (H/R) as an in vitro model of myocardial I/R injury. RESULTS: Cardiomyocyte apoptosis was significantly increased after oxygen-glucose deprivation and reoxygenation (OGD/R), and ALDH2 activation largely decreased the cardiomyocyte apoptosis. Additionally, we found that both ALDH2 activation and overexpression significantly inhibited the increased mitochondrial fission after OGD/R. Furthermore, we found that ALDH2 dominantly suppressed dynamin-related protein 1 (Drp1) phosphorylation (Ser616) and adenosine monophosphate-activated protein kinase (AMPK) phosphorylation (Thr172) but not interfered with the expression levels of mitochondrial shaping proteins. CONCLUSIONS: We demonstrate the protective effect of ALDH2 against cardiomyocyte H/R injury with a novel mechanism on mitochondrial fission/fusion.

The insufficient fund is the"bottleneck"factor restricting the development of the general practitioner education and training in China.The article attempts to investigate how to found a scientific and effective mechanism so as to provide the thinking for transforming the current status.

Objective: To evaluate the quality of Radix bupleuri from different producing areas and make a comparison by using grey correlation analysis(GRA)and principal components analysis(PCA).Methods: Fifteen samples of Radix bupleuri were collected from different producing areas.The percentage of total ash, acid-insoluble ash, extract, saikosaponin a and saikosaponin d of Radix bupleuri from different habitats were determined.The grey correlation quality evaluation model was built for Radix bupleuri from different producing areas and the results were compared with those of PCA.Results: According to the grey correlation analysis on components in the different batches of Radix bupleuri , the samples from Shaanxi province were the most highly correlated with the optimal reference sequence (0.79), which suggested the best quality.Radix bupleuri Fructus from Hebei province was the most poorly correlated with the optimal reference sequence (0.23), which showed the worst quality.The results were basically identical with those of PCA, indicating good repeatability and stability.Conclusion: Grey incidence degree method and the established Radix bupleuri quality evaluation models can be used to evaluate the quality of Radix bupleuri .

Objective To evaluate the safety and efficacy of basic immunization with different sequential immunization programs of oral live attenuated poliomyelitis vaccine(OPV)and inactivated poliomyelitis vaccine(IPV). Methods Infants above two months of age residing in Pudong New Area,Shanghai,were selected for the study,and the basic immunization program of OPV full course(O-O-O group),sequential IPV and OPV(I-I-O group),and IPV full course(I-I-I group)were administered at 2,3 and 4 months of age,respectively. Relevant adverse reactions were proactively monitored by the parents of the participants via completing their own self-observation form after immunization. Before and after immunization,blood samples were collected from the upper arm vein,and the serum was separated. The neutralizing antibody levels of poliovirus in serum were measured by micro neutralization test,and the antibody positive rate and geometric mean titer(GMT)were calculated. Logistic multivariate regression analysis was used to research the effects of group,gender,household registration,birth mass and positive rate of typeⅠ/Ⅲ antibody before immunizationon the positive rate of type Ⅰ/Ⅲ antibody after basic immunization. Results There were one case of lethargy and one case of crying in O-O-O group, one case of lethargy in I-I-O group,and one case of crying in I-I-I group. The GMTs of type I neutralizing antibody in the pre-immunization O-O-O group,I-I-O group,and I-I-I group were 41. 39,8. 21,and 12. 56,and those of type Ⅲ neutralizing antibody were 7. 57,4. 02 and 8. 08,respectively. There was no significant difference in GMT between groups for typeⅠandⅢneutralizing antibodies before immunization(F = 2. 815 and 0. 608,P = 0. 061 and 0. 545,respectively). The positive rates of type Ⅰ antibody before immunization were 24. 04%,34. 07% and 41. 00%,respectively,with significant difference between groups(χ~2= 13. 459,P = 0. 001). The positive rates of type Ⅲ antibody were 13. 94%,9. 89% and 18. 00%,respectively,with no significant difference(χ~2= 5. 188,P = 0. 075). After immunization,the GMTs of type Ⅰ neutralizing antibody were 1 311. 84,1 812. 59 and 833. 24,and those of type Ⅲ neutralizing antibody were 911. 97,1 752. 68 and 419. 50,respectively,with significant differences between groups(F = 76. 848 and 202. 632,respectively,each P < 0. 001). After immunization,the positive conversion rates of type Ⅰantibody were 98. 56%,100% and 100%,and those of type Ⅲ antibody were 99. 04%,100% and 99. 44%,respectively,with no significant difference between the two groups(χ~2= 2. 973 and 1. 045,P = 0. 122 and 0. 789,respectively). In addition,group,gender,household registration,birth mass and positive rate of typeⅠ/Ⅲantibody before immunization had no effect on the positive conversion rate of typeⅠ/Ⅲantibody after basic immunization. Conclusion All three sequential immunization programs achieved good safety and high antibody positive conversion rates after basic immunization.

A considerable proportion of patients with non small cell lung cancer (NSCLC) require postoperative adjuvant therapy,postoperative radiotherapy is an important part of the treatment,this article review the role and value of postoperative radiotherapy for NSCLC patients in recent years.

Objective To investigate the treatment effect and some related fators of the improved lacrimal duct flushing technique for the adult patients with chronic dacryocystitis in the primary hospital.Methods Fifty -seven adult patients (fifty -seven eyes) with chronic dacryocystitis received the improved lacrimal duct flushing treatment.Fifty -seven patients were divided into two groups according to the first time to see a doctor and receive the improved lacrimal duct flushing treatment cooperated with drug application and assistant therapies.The effect and safety of the treatment and some related factors were analyzed.Results There was no statistical difference between the overall efficency of the two groups(A group 76.2%,B group 72.2%,χ2 =0.108,P =0.987 >0.05).The innero-cular pressures showed no statistical difference between pre -treatment and 1 month after treatment(the pre -treat-ment innerocular pressure:18.470,the post -treatment innerocular pressure:18.512,t =-0.315,P =0.754 >0.05).The correlation analysis between the conditions of lacrimal passages and treatment effect(r =0.814,r2 =0.663,P =0.002 <0.05),between the germiculture of the discharge of lacrimal sac and treatment effect(r =0.814, r2 =0.663,P =0.000 <0.05) showed statistical significance.Conclusion The improved lacrimal duct flushing technique cooperated with drug application and assistant therapies is safe and well effective to the adult patients with chronic dacryocystitis in the early stage.

Objective To compare the effect of Zhigancao Decoction and Zhenwu Decoction on hemodynamics, matrix metalloproteinase-9 (MMP-9), tissue inhibitor of metalloproteinase-1 (TIMP-1) and myocardial enzyme of elderly patients with chronic heart failure.Methods 40 elderly patients with chronic heart failure in the first affiliated hospital of the medical college, Shihezi university were selected and randomly divided into Zhigancao Decoction group (n=20) and Zhenwu Decoction group (n =20) .The both of two groups were given conventional treatment, then were treated with respective drug according to different groups with two courses, one course of two weeks.The hemodynamic, MMP-9, TIMP-1 and myocardial enzyme spectrum indexes of two groups were compared post-treatment.Results After treatment, the indexes of hemodynamics in two groups were improved, hemodynamic indexes of Zhigancao Decoction recovered better than those of Zhenwu Decoction (P<0.05) .The serum MMP-9, MMP-9/TIMP-1 of Zhigancao Decoction group were lower and TIMP-1 was higher than those of Zhenwu Decoction group (P<0.05).The myocardial enzyme indexes in both groups were lower post-treatment, and the above indexes in Zhigancao Decoction were lower than those in Zhenwu Decoction group (P<0.05) . Conclusion Zhigancao Decoction could obviously improve the symptoms of chronic heart failure in elderly patients, which has the guiding significant in the clinical treatment.

BACKGROUND:Medical risks are al unsafe events or that damage to the patient during medical services. Present medical risk management is mainly qualitative experience, and lacks of regular failure analysis and risk assessment for established medical treatment.
OBJECTIVE:To construct models of medical process failure analysis and risk assessment, find possible risks during medical treatment, and propose effective measures to eliminate or decrease above risks.
METHODS:Failure mode and effects analysis during reliability engineering were used in production. That was, risk assessment was conducted in the possible technical failure modes, causes and al impacts on the product during each process. Improvement measures were made for weak link during the process. The risk could reach an acceptable level. In accordance with failure mode and effects analysis during production, the procedure of medical process failure analysis and risk assessment could be made to analyze the potential failures during medical treatment. Moreover, the improvement measures were proposed for weak link with high risks so as to prevent the occurrence of risk of significant adverse effects on patients.
RESULTS AND CONCLUSION:The methods and basic procedures of medical process failure analysis and risk assessment were established by using the experience of failure mode and effects analysis. Taking the rescue process of myocardial infarction in the emergency of a hospital as an example, the analysis of failures, reasons and impacts was performed taking“chewing 300 mg aspirin”in the rescue steps as a key. The improvement measures and suggestions were proposed for unacceptable failures and reasons. Seen from the analysis results, proposed improvement measures and suggestions can obviously decrease the risks of failures caused by this step to patients. Therefore, the application of failure mode and effects analysis in medical treatment has a strong practical value.

Abstract BACKGROUND: Recently, microcarrier culture as a high concentration cel culture technology has been used in hepatocyte culturein vitro. OBJECTIVE:To periodicaly observe the morphological changes of human hepatocyte L-02 cultured by the spherical porous chitosan microcarrier. METHODS:The self-made spherical porous chitosan microcarrier sample as a support to culture human hepatocytes L-02 acted as experimental group; non-spherical porous chitosan microcarrier as a support to culture human hepatocytes L-02 as control group. Cels in two groups underwent cel count at regular time, and the morphological changes were observed in the experimental group, including inverted phase contrast biological microscope observation and scanning electron microscope observation. RESULTS AND CONCLUSION:The quantity of cultured cels in the two groups was increased in the first 3 days and reached the peak at the 3rd day. The cel quantity in the experiment group was obviously higher than that in the control group (P < 0.05). There was no significant difference in the three samples of the experimental group (P > 0.05). The quantity of hepatocytes adhered to the microcarrier surface was gradualy increased in the former 3 days under the inverted phase contrast biological microscope. There were lots of cel clusters on the surface of a greater part of microcarriers, with the total survival rate of more than 90%, and hepatocytes kept a good morphological structure. Under the scanning electron microscope, lots of hepatocytes adhered tightly to each other on the surface and section of microcarrier as wel as inside the microcarrier. It is indicated that the self-made spherical porous chitosan microcarrier as a support in three-dimensional environment can undergo cel culture with high concentration.

Objective To investigate the expression and significance of DLL4 in endometriosis.Methods 83 cases due to ovarian cyst surgery from December 2010 to September 2013,were selected in the Second Affiliated Hospital of Zhengzhou University,and were randomly divided into two groups in accordance with the random number table.Group A:48 cases were induded after staining from 83 patients who were tahen a part of cyst wall;Group B:of laparoscopic ovarian cyst surgery in 83 patients,37 cases of simultaneously hysteroscopy due to fertility requirements,exclude endometrial lesions,were taken reign uterine endometrium.The American fertility association revised standard (r-AFS)in 1985 was used to divide the clinical stage:Group A:21 cases of Ⅰ ~Ⅱ stage,27 cases of Ⅲ ~Ⅳ;Group B:16 cases of Ⅰ ~Ⅱ stage,21 cases of Ⅲ ~Ⅳ stage;Group C:30 cases hysteroscopic uterine septum resection with electricity due to lower mediastinal uterus were selected at the same period.The expression levels of DLL4 protein in Group A,B were detected by S-P method,and compared with the control group (Group C),then analyze its relationship with EMs stage. Results In Group A,B and C,the expression of DLL4 protein were (144.99 ±10.12 ),(1 15.42 ±10.39 ),(90.08 ±7.29 ),and there were significantly diffiences among three groups (F=314.220,P<0.05);The expression of DLL4 protein atⅠ~Ⅱstage were significantly lower than that inⅢ~Ⅳ stage (P<0.05).Conclusion DLL4 protein may play an important role in the pathogenisis of endometriosis.