Objective To explore the effectiveness of defluoridization of water and the morbidity trends in endemic fluoroais areas in Yanqing county of Beijing, in order to provide the basis for the formulation of prevention and control measures. Methods In 2008, stratified random was used to collect water samples to detect the amount of drinking water fluoride in three villages(Gujiaying, Bojiying and Hujiaying) in Yanqing county using ion-selective electrode in both dry and rainy seasons. Sixty-three students in 8 - 12 years old and 664 adults over 16 years of age were determined of urinary fluoride, and examine the prevalence of dental fluorosis. ResultsThe fluorine content in drinking water in the dry and rainy seasons was (0.72 ± 0.12), (0.76 ± 0.18)mg/L, respectively. The median of fluorine content in urine of children was 1.11 mg/L with the range of 0.32 - 4.15 mg/L; the median of fluorine content in urine of adult was 1.42 mg/L with the range of 0.37 - 4.65 mg/L. The detection rate of dental fluorosis in children was 23.81%(15/63), dental fluorosis index was 0.45; the detection rate of dental fluorosis in adult was 54.97% (365/664), dental fluorosis index was 1.70. Conclusions Fluorine content in drinking water meets the national standard in Yanqing county. The morbidity of endemic fluorosis descends in some extent in a result of defluoredization.

Objective To understand current status of brucellosis infection in both human and animal in Yanqing County to get solid scientific basis for controlling the disease and to prevent the spread of epidemic.Methods Cluster sampling method was used in this survey in stock raisers and ELISA and SAT were performed to inspect serologic antibody of Brucella.At the same time an epidemic investigation was carried out,including the basic personal situation,occupational history,vaccination history,clinical symptom,and so on.Resutls In a total of 216 cow raisers whose blood Was detected,27 cases showed positive in titer test of Brucella antibody with a ratio of 12.50%.However,all the antibody carriers did not have any clinical symptoms.In all 2185 cows that were collected for antibody test,1488 cases had positive serological antibody with a ratio of 68.10%.And all the positive COWS also did not have any clinical symptoms.Conclusions The subclinical infection of brucellosis is rather high in Yanqing County's stock raisers.It is self-protection of the stock raisers,epidemic knowledge propaganda,serological surveillance in human and animal,immunize of healthy cows,and eliminate of diseased cows that are urgent in order to keep the public in health.

70% . The patients were mechanically ventilated. VT was set at 10 ml? kg-1 and respiratory rate at 12 bpm. End-tidal PCO2 was maintained at 30-35 mm Hg. End-tidal PCO2, SpO2, ECG and BP were continuously monitored. The respiratory function and mechanics were measured using respiratory monitor CP-100 (BICORE) before and after pneumoperitoneum (intra-abdominal pressure reached 15 mm Hg) and 5,10,15,20,25,30,35 min after vecuronium. The respiratory parameters measured included inspiratory and expiratory VT ( VTi, VTe) , minute ventilation (VE), respiratory rate (RR), peak inspiratory and expiratory flow rate (PIFR,PEFR), PEEP, auto-PEEP, pressure-time product (PTP), inspiratory-time ratio (TI/TTOT ), rate/VT ratio, average airway pressure (PAWM), esophageal pressure (PES), peak inspiratory pressure (PIP), dynamic compliance (CDyn), airway resistance (RAw ) and work of breathing. Results There was no significant difference in the effect of different doses of vecuronium on respiratory function and mechanics including all parameters measured at all time points among the three groups. Mean airway pressure, esophageal pressure peak inspiratory pressure, airway resistance and work of breathing increased significantly but dynamic compliance decreased significantly after CO2 penumoperitoneum. Conclusion Intra-abdominal CO2 insufflation significantly attect respiratory mecnanics. me effect of pneumoperitoneum on respiratory mechanics can not be prevented or attenuated by increasing the dose of muscle relaxant.

To explore the regulation of eIF4E, we screened the protein interacting with eIF4E from human cDNA library by using yeast two-hybrid system. Several clones interacting with eIF4E were identified. One of them was homologous with HUWE1 (HECT, UBA and WWE domain containing 1, also named as ARF-BP1, HECTH9 or HUWE1). Cell co-immunoprecipitation showed that eIF4E could bind to HUWE1 in mammalian cells. We also found that HUWE1 bearing the HECT domain is necessary for its association with eIF4E.
Animals ; Eukaryotic Initiation Factor-4E ; metabolism ; Humans ; Ubiquitin-Protein Ligases ; metabolism

Object To develop an improved RP-HPLC method for determining ferulic acid in the human serum. Methods The determination was carried on RP-HPLC, using Kromasil-C 18column, methanol-water-acetic acid (36.4∶63∶0.6) as mobile phase with a flow rate of 1.0 mL/min and at the detection wavelength of 322 nm, and p-hydroxybenzaldehyde was used as internal standard. The serum sample was deproteinized with acetonitrile to extract ferulic acid. Results The calibration curve showed good linearity over the range of 9.94-159.04 ng/mL (r=0.992 5). RSD was less than 10% within day and day-to-day, the average recovery was 99.77% and the minimal concentration in serum was 5 ng/mL. Conclusion This method, which is simple, rapid, sensitive, reproducible and low toxic, is appropriate for the quantitative determination of ferulic acid in the human serum.

Objective To establish a rapid monitoring method of the three common bacteria in mice frozen resources, such as embryo, sperm, etc. Methods To extract DNA of the three positive bacteria( Staphylococcus auerus, Klebsiella?pneumoniae and β?hemolyticstreptococcus) , and establish PCR monitoring method of the three positive strains through designing primer and refining PCR condition. Then extract total DNA of the frozen resources, detect the DNA according to the PCR condition of the three positive bacteria, some samples were detect by fluorescence quantitative PCR at the same time. Results ①successfully establish a PCR detection method of the three positive bacteria, the minimum detectable concentration of Staphylococcus auerus, Klebsiella pneumoniae and β?hemolytic streptococcus is 4?19 × 10 -5 ng/μL, 1?98 × 10 -5 ng/μL and 1?07 × 10 -3 ng/μL. ②Proved that the three bacteria doesn ’ t exist in the sample by normal PCR and fluorescence quantitative PCR methods. Conclusions Establising a rapid monitoring method of common pathogens of frozen embryo and sperm in mice.

Objective To investigate the changes of average body weight gain and serum biochemical indexes of C57BL/6J mice (B6 mouse) and their offspings after frozen-thawed embryo transfer of B6 mice.Methods The mice were divided into three groups in this study.In the experimental group I (E-I,30 males and 20 females),2-cell embryos after in-vitro fertilization were collected,and cryopreserved by EFS method,then obtained the offsprings after transplantation of the recovered embryos to oviduct of recipient mice (ICR mouse).In the experimental group II (E-II,26 males and 17 females),when the mice from E-I grew to maturity,the offsprings were obtained from natural mating of mice from E-I.In the control group (20 males and 20 females),the offsprings came from conventional feeding and natural mating.The three groups of mice were raised to 16 weeks old,weighing the body weight at a regular time intervals,and the serum biochemical indexes were obtained from 16-week-old mice.Then the changes of average body weight and serum biochemical indexes of the mice were analyzed.Results The average body weight of E-I mice was significantly higher than that of control group at each week-age (P<0.01).The average body weight of E-II female mice was significantly higher than that of the control group in 12-16-week old mice (P<0.01),but the average body weight of E-II male mice showed no significant differences compared with the control group except for few weeks.The serum biochemical indexes of E-I and E-II mice were changed in all items except for AST,TP and Ca.Conclusions There are some effects on the average body weight gain and serum biochemical indexes of C57BL/6J mice and their offspings after frozen-thawed embryo transfer.

OBJECTIVETo explore the mechanism of Shugan Qingzhi Granule (SGQZG) for reducing blood lipids level and anti-atherosclerosis.

METHODSHyperlipidemia rabbit model was established by high-fat diet. Sixty New Zealand rabbits were randomly divided into 6 groups, the model group (A), the high and low dose SGQZG group (B and C), the Chinese medicine control group (D), the Western medicine control group (E), and the normal group (F), 10 in each group. The effect of SGQZG on vascular endothelial functions was determined by biochemistry and radioimmunoassay; activity of oxidized low-density lipoprotein (ox-LDL) and tumor necrosis factors CD40/CD40L analyzed by ELISA; and matrix metalloproteinase-2 and -9 (MMP-2 and MMP-9) detected using RT-PCR.

RESULTSAs compared with the model group (Group A) the ox-LDL level was lower in Group B-F, MMP-2 and MMP-9 were lower and TIMP-2 was higher in Group B, C and E, all showed statistical significance (P <0.01).

CONCLUSIONSGQZG may alleviate and regress the genesis and development of atherosclerosis through preventing the adhesion of inflammatory cells, inhibiting proliferation and migration of smooth muscle cells, and reducing the production of extracellular matrix to stabilize the plaque.

Animals ; Atherosclerosis ; blood ; drug therapy ; pathology ; Drugs, Chinese Herbal ; therapeutic use ; Endothelium, Vascular ; drug effects ; physiopathology ; Enzyme-Linked Immunosorbent Assay ; Female ; Lipids ; blood ; Lipoproteins, LDL ; blood ; Male ; Matrix Metalloproteinase 2 ; blood ; genetics ; Matrix Metalloproteinase 9 ; blood ; genetics ; Phytotherapy ; Rabbits ; Random Allocation ; Reverse Transcriptase Polymerase Chain Reaction

Objective To compare the effects of different transplantation sites on the outcome of testicular grafts in mice, and to provide a basis for development and application of this technique in relevant research .Method 5-day old and 4-week old SPF male C57BL/6J mice were used in this study .Three groups of testicular transplantation , i.e.dorsal subcutaneous transplantation ( 5 mice, 40 testes ) , transplantation inside the testicular tunica albuginea ( 6 mice, 12 testes), and were subrenal capsule transplantation (10 mice,15 testes) groups were set up for evaluating the effect of transplantation site on the outcomes in mice .Sham operation (4 mice) and castration (4 mice) groups were also used in this study.The mice were sacrificed at 8 weeks after transplantation and the transplanted testes were collected for analysis of their weight, transplant survival, weight gain, and germ cell differentiation.Results There were significant differences of the testicular germ cell differentiation in different transplantation groups .The germ cell differentiation was best in the in-tra-tunica albuginea transplantation group , and were similar to that in the sham operation group .The germ cell differentia-tion rate was 100%in the intra-tunica albuginea transplantation group , 29.2% in the subcutaneous transplantation group and 0%in the subrenal capsule transplantation group .Conclusions Transplantation beneath the testicular tunica albug-inea is the most favorable site for germ cell differentiation , and dorsal subcutaneous transplantation is an alternative choice . Subrenal capsule transplantation is not appropriate for preservation of male reproductive organs in mice .

Biological chemistry of Chinese herbal germplasm resources (BCCHGR) is a new cross-discipline formed from rapid development of modern science and technology and its application in the area of Chinese herbal resources. BCCHGR was defined as probing and understanding biological processes like heredity, gene transcription, expression and metabolism of Chinese herbal germplasm, at the interface of biochemistry, molecular biology and chemistry, elu-cidating the nature of Chinese herbal germplasm using as TCM medicine as well as the forming mechanism thereof. In this paper, the scientific background, definition, significance and contents of BCCHGR were discussed to depict a preliminary picture of BCCHGR and arouse popular consideration and discussions.