1.Echocaridiographic evaluation on early left ventricular remodeling after acute myocardial infarction: experimental study
Jian, WANG ; Chun-song, KANG ; Yan-ping, ZHENG ; Ji-ping, XUE ; Rui, ZHANG
Chinese Journal of Medical Ultrasound (Electronic Edition) 2008;5(2):229-234
Objective To investigate the onset of left ventricular remodeling (LVRM) after acute myocardium infarction (AMI) and its changes within 6 hours in dogs on echocardiography. Methods AMI was induced in 14 dogs by ligating the left anterior descending arteries. Eight myocardium infarcted models were successful and were sacrified for pathological study. The indices of LVRM: wall infarction thickness (WIT), the wall motion score index (WMSI), left ventricular end-diastolic volume (LVEDV), left ventricular end-systolic volume (LVESV) and left ventricular ejection fraction (LVEF) were evaluated before and 1 h, 2 h, 3 h, 4 h, 5 h and 6 h after operation. Results Compared with the pre-operation, WIT and LVEF were decreased (P<0.01), LVESV and WMSI were increased (P<0.01), and LVEDV was increased (P<0.05 or P<0.01) at every time point after operation. WIT had no significant difference at 1 h, 2 h, 3 h, 4 h, 5h and 6h after operation (P>0.05). LVEDV, LVESV were higher (P<0.05) and LVEF was lower (P<0.05 or P<0.01) at 4 h, 5 h, and 6 h than at 1 h, and 2 h after operation. WMSI was higher at 3 h, 4 h, 5 h, and 6 h than at 1h (P<0.05). Conclusions In our experiment, LVRM occurred at 1 h after AMI in dogs. Thus echocardiography may evaluate early LVRM.
2.Diagnostic value of endobronchial ultrasound-guided transbronchial needle aspiration in elderly patients with bronchogenic carcinoma
Kui XIAO ; Jiehan JIANG ; Shulan XIA ; Naixin KANG ; Zhihui SHI ; Rui ZHOU ; Ping CHEN
Chinese Journal of Geriatrics 2014;33(9):958-960
Objective To evaluate the diagnostic value and safety of endobronchial ultrasoundguided transbronchial needle aspiration (EBUS-TBNA) in elderly patients with bronchogenic carcinoma.Methods From August,2013 and July,2014,31 patients aged 65 years and over with space-occupying mass and enlarged mediastinal lymph nodes detected by CT or positron emission tomography-computed tomography (PET-CT) underwent EBUS-TBNA.Rapid onsite cytology evaluation was not performed.Results There were 26 males and 5 females in this study,aged 65-77 years (70.1 years old on average).In 31 patients,70 samples were obtained from lymph nodes (LNs) and 4 samples were obtained from intrapulmonary lesions.29 cases were diagnosed as lung cancer,and 2 cases had false-negative diagnoses.The sensitivity and specificity rates of EBUS-guided TBNA method were 93.6% and 100.0%,respectively.No major complications were observed in this series.Conclusions EBUS-TBNA is an safe and effective method in diagnosing bronchogenic carcinoma in elderly patients.
3.Detection of bone marrow involved by nonhematopoietic neoplasms using flow cytometry
Hui WANG ; Xin YU ; Jirun PENG ; Chunrong TONG ; Ping WU ; Yanyan LI ; Rui KANG ; Huipeng SUN
Chinese Journal of Laboratory Medicine 2011;34(2):141-146
Objective To study the value of flow cytometry in identifying metastatic CK positive and negative nonhematopoietic neoplasms in bone marrow. Methods Twenty-six cell lines representing ten epithelial neoplasms, one lymphoma cell line and one human T cell lymphoblast-like cell line were purchased from American Tissue Culture Collection. From July 2009 to June 2010, five nonhematopoietic neoplasms,fifteen hematopoietic neoplasms and fifteen control patients with complete remession after hematopoietic stem cell transplantation were collected in Beijing Daopei Hospital. Cryopreserved cell lines were thawed and cultured until they entered log phase. After permeabilization, cell lines were analyzed by staining with cytoplasmic CK-FITC antibody using four-color flow cytometer. The percent CK positivity was measured by comparing with negative control. Bone marrow samples were stained with membrane and cytoplasmic antibodies according to our routine methods. Based on lineage markers and blast markers as well as CK expression, the relevant hematopoietic diseases were diagnosed or excluded according to 2008 World Health Organization diagnosis standards. Results All epithelial neoplasm cell lines expressed CK, with average positive percentage 81.1%. All the lymphoid tumor cell lines didn't express CK. Two epithelial neoplasms were CK positive, 100. 0% in thyroid carcinoma and 98. 2% in lung carcinoma, respectively. Hematopoietic tumor and control samples didn't express CK. They expressed relevant hematopoietic markers, such as CD45 as well as lineage markers, or CD138 and cytoplasmic immunoglobulin light chain. Three nonepithelial nonhematopoietic neoplasms didn't express CK. CK positive or negative nonhematopoietic neoplasms didn't express hematopoietic markers such as CD45, HLA-ABC and HLA-DR DP DQ, as well as lineage specific markers. Besides, CK positive might be helpful to suggest epithelial origin. Conclusion Flow cytometry with hematopoietic markers and CK can effectively exclude hematopoietic tumor and identify metastatic CK positive and negative nonhematopoietic neoplasms in bone marrow.
4.Intervention effect of Tibetan patent medicine Ruyi Zhenbao pills in acute ischemic stroke in rats.
Rui-ying LIU ; Wei-jie WU ; Rui TAN ; Bin XIE ; Zhen-dong ZHONG ; Jing-ping HE ; Yao CHEN ; Xin-li KANG
China Journal of Chinese Materia Medica 2015;40(3):556-559
Ischemic stroke is a primary cause of death and long-term disability all over the world. This disease is resulted from ischemia and hypoxia in brain tissues because of insufficient blood supply and causes a series of physiochemical metabolism disorders and physiological dysfunction. Its high disability ratio has bright huge burdens to society, governments and families. However, there is not efficacious medicine to treat it. In this study, a right middle cerebral artery occlusion was established in rats to observe the multi-path and multi-aspect intervention effects of Tibetan patent medicine Ruyi Zhenbao pills in reducing injuries to Nissl bodies, cerebral edema and inflammatory reactions and preventing cellular apoptosis, in order to lay a foundation for defining its therapeutic mechanism in acute ischemic stroke.
Animals
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Brain Ischemia
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drug therapy
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Male
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Medicine, Chinese Traditional
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Medicine, Tibetan Traditional
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NF-kappa B
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physiology
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Patents as Topic
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Rats
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Rats, Sprague-Dawley
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Stroke
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drug therapy
5.Correlation of gene expression related to amount of ginseng saponin in 15 tissues and 6 kinds of ginseng saponin biosynthesis.
Kang-yu WANG ; Mei-ping ZHANG ; Chuang LI ; Shi-cui JIANG ; Rui YIN ; Chun-yu SUN ; Yi WANG
China Journal of Chinese Materia Medica 2015;40(16):3168-3173
Fifteen tissues of 4-year-old fruit repining stage Jilin ginseng were chosen as materials, six kinds of monomer saponins (ginsenosides Rg1, Re, Rb1, Rc, Rb2 and Rd) content in 15 tissues was measured by HPLC and vanillin-sulfuric acid method. The relative expression of FPS, SQS, SQE, OSC, β-AS and P450 genes in 15 tissues was analyzed by real-time PCR. The correlations between ginseng saponin content in 15 tissues of Jilin ginseng and biosynthetic pathway -related genes were obtained. The results showed that was a synergistic increase and decrease trend of positive linear correlation among six kinds of monomer saponin content, and there was a significantly (P < 0.01) positive correlation between monomer saponin content and total saponins content. Monomer saponin content and 6 kinds of enzyme gene correlation were different. Biosynthesis of ginseng total saponins and monomer saponin were regulated by six kinds of participation ginsenoside biosynthesis enzyme genes, the expression of these six kinds of genes in different tissues of ginseng showed collaborative increase and decrease trend, and regulated biosynthesis of ginseng ginsenoside by group coordinative manner.
Drugs, Chinese Herbal
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analysis
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Gene Expression Profiling
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Panax
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chemistry
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genetics
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metabolism
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Plant Proteins
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genetics
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metabolism
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Plant Structures
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chemistry
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genetics
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metabolism
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Plants, Medicinal
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chemistry
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genetics
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metabolism
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Saponins
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analysis
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metabolism
6.Analysis of Pathological Biopsies in 1782 Cases in Dongguan Region
yu-feng, LI ; jian-fang, HE ; xu-ming, LIU ; shu-rui, XIE ; dong-ping, KANG ; xue-wu, TIAN
Journal of Applied Clinical Pediatrics 2006;0(13):-
Objective To study the onset features and axiom of children disease in Duanguan region.Methods A statistic study was carried on 1 782 cases of children disease which confirmed by pathological examinations.The sex,age and pathology(congenital malformation,inflammatory disease,tumor-like lesions,benign tumors,malignant tumors)were registered.Results The inflammatory disease occupied the first place in incidence rate(44.61%),followed by tumor-like lesions(25.03%),benign tumors(23.34%),congenital malformation(5.22%)and malignant tumors(1.80%).Among inflammatory disease,appendicitis was the most common,the next was chronic tonsillitis and tuberculosis.Among tumor-like lesions,cystic disease was the most common;the next was polyps.Among benign tumors,vasculoma was the most common,the next was pilomatricoma,osteoid and chonodroid tumors.Among congenital malformation,ear fistula was the most common,the next was diverticulosis and intestineal duplicateion.Among the malignant tumors,the sarcoma of soft tissue was the most common,the next was the malignant peripheral nerve tumors and lymphoma.Conclusion Children's disease have its own characteristics which varied in age,sex and pathological types,and differ from those of adults.
7.Qualitative analysis of chemical constituents in Si-Wu Decoction based on TCM component database.
Zhen-fang WANG ; Yang ZHAO ; Zi-quan FAN ; Li-ping KANG ; Li-rui QIAO ; Jie ZHANG ; Yue GAO ; Bai-ping MA
Acta Pharmaceutica Sinica 2015;50(10):1309-1317
In order to clarify the chemical constituents of Si-Wu Decoction rapidly and holistically, we analyzed the ethanol extract of Si-Wu Decoction by UPLC/Q-TOF-MSE and UNIFI which based on traditional Chinese medicine database, the probable structures of 113 compounds were identified. The results show that this method can rapidly and effectively characterize the chemical compounds of Si-Wu Decoction and provide a new solution for identification of components from complex TCM extract.
Chromatography, High Pressure Liquid
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Databases, Pharmaceutical
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Drugs, Chinese Herbal
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chemistry
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Medicine, Chinese Traditional
8.Expression and clinical significance of matrix metalloproteinase in gastric stromal tumor.
Rui-zheng MIAO ; Yue-ping LI ; Yong-kang WANG ; Xiao-bo YU ; Min ZHANG
Chinese Journal of Surgery 2007;45(17):1196-1198
OBJECTIVETo investigate the expression and clinical significance of matrix metalloproteinase (MMP) in gastric stromal tumor (GST).
METHODSMMP-2 and MMP-9 expression were determined by immunohistochemistry in tumor tissues in 44 patients with GST, and their relationship with clinicopathologic factors of the neoplasm was also investigated.
RESULTSMMP-2 and MMP-9 were expressed in the cytoplasm in 84.1% (37/44) and 81.8% (36/44) of tumors, respectively. The positive rates of MMP-2 and MMP-9 increased significantly in parallel to the increase in tumor malignancy (P < 0.05) and associated with pattern of tumor growth, tumor size, and centre necrosis (P < 0.05). In addition, there was a statistically significant positive correlation between the expression of the two markers in GST (r = 0.6523, P < 0.05). Furthermore, the 5-year postoperative survival rates of patients with positive expressions of MMP-2 and MMP-9 were significantly lower than those of patients with negative expressions of the two markers (P < 0.05).
CONCLUSIONOver expression of MMP-2 and MMP-9 can be served as objective markers to judge the malignant degree and, to predict the prognosis of patients with GST.
Adult ; Aged ; Female ; Follow-Up Studies ; Gastrointestinal Stromal Tumors ; enzymology ; pathology ; Humans ; Immunohistochemistry ; Kaplan-Meier Estimate ; Male ; Matrix Metalloproteinase 2 ; biosynthesis ; Matrix Metalloproteinase 9 ; biosynthesis ; Middle Aged ; Prognosis ; Stomach Neoplasms ; enzymology ; mortality ; pathology ; Survival Rate
9.Serum IgA against type 3 muscarinic acetylcholine receptor is a novel marker in diagnosis of Sjögren's syndrome.
Ying-Ni LI ; Jian-Ping GUO ; Jing HE ; Xia LIU ; Fang-Rui YIN ; Yan DING ; Hai-Hong YAO ; Ai-Jun KANG ; Zhan-Guo LI
Chinese Medical Journal 2011;124(16):2490-2495
BACKGROUNDAntibodies against type 3 muscarinic acetylcholine receptor (M3R) are involved in the pathogenesis of Sjögren's syndrome (SS), but the clinical value of them in SS patients has been controversial. The aims of this study were to: (1) establish an improved enzyme-linked immunosorbent assay (ELISA) to detect IgA antibodies against M3R; (2) evaluate the value of IgA antibodies against the second extracellular loop of M3R205-220 (c2M3RP) in diagnosis of SS.
METHODSTo increase the ELISA sensitivity, c2M3RP was coupled to bovine serum albumin (BSA) by the glutaraldehyde method and a 96-well microplate was treated by ultraviolet rays before coated. Concentrations of anti-c2M3RP, anti-SSA, and anti-SSB were measured in the sera of 240 individuals: 91 patients with primary SS and 149 controls (16 secondary SS, 27 systemic lupus erythematosus, 40 rheumatoid arthritis and 66 healthy controls). Diagnostic properties of anti-c2M3RP were determined by receiver-operating characteristic curve analysis.
RESULTSThe prevalence of serum IgA anti-c2M3RP antibodies in patients with pSS (46%, 42/91) was significantly higher than that in patients with systemic lupus erythematosus (19%, 5/27), in rheumatoid arthritis (15%, 6/40) and in healthy controls (5%, 3/66). However, there was no significant difference between the two SS groups (P = 0.727). The diagnostic performance of IgA anti-M3RP antibodies was similar to anti-SSA assay, but had 22% higher sensitivity than anti-SSB. By analyzing of IgA anti-c2M3RP antibodies, combination of anti-SSA and anti-SSB resulted in increased sensitivity, whereas their specificity was not significantly changed.
CONCLUSIONSThe improved anti-c2M3RP ELISA is a novel, sensitive, and specific serological test for the diagnosis of SS. The combined application of anti-c2M3RP, anti-SSA and anti-SSB tests can improve the laboratory diagnosis of SS. The IgA anti-c2M3RP antibodies may serve as a novel diagnostic marker for SS.
Adult ; Aged ; Aged, 80 and over ; Animals ; Autoantibodies ; immunology ; Biomarkers ; blood ; Cattle ; Enzyme-Linked Immunosorbent Assay ; methods ; Female ; Humans ; Immunoglobulin A ; blood ; Male ; Middle Aged ; Receptors, Muscarinic ; immunology ; Sjogren's Syndrome ; blood ; Young Adult
10.Chorionic villus cell culture and karyotype analysis in 110 cases of early spontaneous abortion.
Li XIONG ; Jie LIU ; Kang DENG ; Si-ping LIU ; Bei JIA ; Rui-feng WU ; Mei ZHONG ; Rong ZENG
Journal of Southern Medical University 2009;29(1):64-67
OBJECTIVETo investigate the value of fetal chromosomal karyotype analysis in cases of early spontaneous abortion.
METHODSChorionic villus specimens obtained from 110 cases of early spontaneous abortion were cultured for karyotype analysis.
RESULTSOf the 110 cases, chorionic villus was successfully cultured in 103 cases (93.7%), and abnormal embryo karyotypes were identified in 52 cases (50.5%). Trisomy was the most frequent embryo karyotype abnormalities in these cases, and chromosomal aberration occurred in 29 cases (52.9%) of the first spontaneous abortion and in 23 cases (42.6%) of repeated abortions. Female fetuses accounted for 75.5% (78 cases) in the spontaneously aborted fetuses and for 67.3% (35 cases) in fetuses with chromosomal abnormalities.
CONCLUSIONEmbryo chromosomal abnormality is the most important reason of early spontaneous abortion, and karyotype analysis of the villus helps identify the causes of abortion and ensure the success of the next pregnancy.
Abortion, Spontaneous ; genetics ; pathology ; Adult ; Cells, Cultured ; Chorionic Villi ; pathology ; Female ; Humans ; Karyotyping ; Pregnancy ; Pregnancy Trimester, First ; Trisomy ; genetics