Objective:To explore the effects of acupoint massage combined with transcranial direct current stimulation on cognitive function and quality of life in patients with spontaneous cerebral hemorrhage, to provide direction for early nursing intervention of patients with spontaneous cerebral hemorrhage.Methods:A total of 214 patients with spontaneous cerebral hemorrhage in First Affiliated Hospital of Zhengzhou University from February 2018 to May 2020 were selected as the research objects. They were divided into 2 groups by random number table method: control group (107 cases) and observation group (107 cases). The control group was given transcranial direct current stimulation intervention, and the observation group was given acupoint massage intervention on the basis of the control group. The intervention lasted for 3 weeks. The intervention effects, cognitive function and quality of life of the two groups were evaluated by National Institutes of Health Stroke Scale, Montreal Cognitive Assessment, Stroke Specific Quality of Life Scale and compared.Results:The total effective rate was 91.59%(98/107) in the observation group, 80.37%(86/107) in the control group, and the difference was statistically significant ( χ2 value was 5.583, P<0.05). There was no significant difference in the cognitive function and quality of life before intervention between the two groups ( P>0.05). The visual space and execution, language, abstraction, delayed recall, orientation and total scores after intervention were (2.54 ± 0.51), (2.35 ± 0.37), (1.96 ± 0.31), (2.78 ± 0.38), (2.88 ± 0.41), (17.48 ± 2.01) points in the observation group, and (2.30 ± 0.62), (2.13 ± 0.36), (1.54 ± 0.38), (2.25 ± 0.43), (2.52 ± 0.38), (15.66 ± 1.89) points in the control group, and the differences were statistically significant ( t values were 3.092-9.554, P<0.05). The energy, family role, mood, personality, language, activities, self-care ability, social role, thinking, upper limb function, vision, work/labor and total scores after intervention were (12.85 ± 2.16), (12.94 ± 3.17), (23.46 ± 3.87), (12.53 ± 3.02), (23.76 ± 4.03), (27.52 ± 4.48), (24.92 ± 3.27), (20.94 ± 4.07), (13.15 ± 2.00), (24.54 ± 4.30), (12.20 ± 2.58), (13.54 ± 2.38), (222.35 ± 24.38) points in the observation group, and (10.04 ± 2.02), (10.52 ± 3.03), (21.25 ± 4.02), (11.15 ± 3.00), (21.46 ± 3.31), (24.37 ± 4.32), (22.38 ± 3.05), (18.60 ± 4.02), (12.47 ± 2.05), (22.08 ± 4.21), (11.42 ± 2.63), (11.05 ± 2.51), (197.49 ± 20.06) points in the control group, and the differences were statistically significant ( t values were 2.190-9.829, P<0.05). Conclusions:Acupoint massage combined with transcranial direct current stimulation can improve the cognitive function and quality of life of patients with spontaneous cerebral hemorrhage, and the effect is definite.

Objective To assess the anticonvulsant effect of midazolam, thiopental and propofol on the convulsion induced by ropivacaine in rats and the underlying mechanism.Methods Fifty male SD rats, 4-6 months of age, weighing 250-300 g were randomly divided into five groups of 10 animals each : (1) control group (C); (2) ropivacaine group; (R) (3) midazolam-ropivacaine group (MR); (4) thiopental-ropivacaine group (SR) and (5) propofol-ropivacaine group (PR) . In control group normal saline 2 ml was infused i.v. . In ropivacaine group (R) 0.75% ropivacaine was infused i.v. at 0.5 ml?min-1 until convulsion occurred. In midazolam - ropivacaine group (MR) midazolam 0.23 mg?kg-1 was injected i.v. 3 min before 0.75% ropivacaine infusion. In SR and PR groups thiopental 2 mg?kg-1 or propofol 1 mg?kg-1 was injected i.v. 1 min before 0.75% ropivacaine infusion. As soon as convulsion occurred ropivacaine infusion was stopped. The animals were decapitated and their brains were removed and kept in liquid nitrogen for determination of the contents of excitatory ammo-acids (aspartic acid and glutamic acid) and the inhibitory amino-acids [glycine and ?-amino butyric acid (GABA)] by high-performance liquid chromatography . The amount of ropivacaine infused in each group was recorded and calculated.Results The dose of ropivacaine needed to produce convulsion was 4.6?0.5 mg?kg-1 in group R and was significantly increased in the 3 groups pretreated with general anesthetics and was 2.96 times the dose in group R in MR group, 2.84 times in SR group and 1.54 times in PR group. The 4 amino-acid concentrations in the brain were significantly higher in group R than in control group. Compared to group R the Asp, Gly and GABA concentrations were significantly lower in group MR and PR but in group SR only Asp concentration was significantly lower. There was no significant difference in glutamic acid concentration among the4 roopivacaine groups (group 2-5).Conclusion Midazolam, thiopental and propofol can all increase the dose of ropivacaine needed to induce convulsion and midazolam and thiopental are more effective than propofol.

Objective To explore the current status of sexual education and real needs of college freshmen in Kunming.Methods By applying random sampling,350 students from three universities in Kunming were investigated by seff-administered questionnaire,and 310 valid questionnaires were recovered.Fesults 23.9％ students confirmed that the schools do not provide courses related to sexual health,and 22.9％ of the surveyed students claimed that they even did not heard any sexual education courses in their schools.The main sources of students to acquire information in terms of sexuality and sexual health are network television,video magazines,friends and classmates.Both male and female students are more acceptable to carry out sex education lectures.There were significant differences between male and female students in the form of obtaining sexual education,the content of sexual education and the demand of sexual education in three aspects (P＜0.05).Girls tend to carry out sexual education in the form of major courses,but boys tend to the form of playing video or distributing books and periodicals (P＜0.05).Girls tend to obtain the courses on sexually disease,AIDS,heterosexual intercourse,or love psychology,but boys tend to contraceptive knowledge and skilIs,or Sexual value orientation (P＜0.05).Girls are more likely to acquire the knowledge of masturbation,sexual health,or sexual health,but boys prefer to the knowledge of sexual psychology,parasexuality,or homosexuality (P＜0.05).Conclusion As a main body of educational institute for young people,all universities should provide training courses related to sexuality and sexual health at the early stage of higher education in order to equip college students with essential knowledge and skills to have safer and positive impact on their college studies.

Objective To investigate the influence of different hemostatic way on ovarian function in laparoscopic excision of ovarian cysts. Methods 87 cases with unilateral ovarian cysts by laparoscopic excision were chosen from Jun. 2012 to Jun. 2013 in our hospital, and they were divided into the electrocoagulation group (44 cases) and the suture group (43 cases) according to hemostatic way. The electro-coagulation group were given bipolar coagulation while the suture group were given endoscopic hemostasis. The ovarian hormone levels and blood parameters before and after surgery were compared between the two groups. Results There was no statistically significant differenc in parameters of E2,FSH,LH,P before surgery between the two groups (P>0. 05), and there was no statistically significant differenc before and after surgery in the same group (P>0. 05). Compared with the suture group, there were statistically significant difference in terms of pa-rameters of E2,FSH in the electrocoagulation group(P<0. 05). There was no statistically significant difference in ovary sectional area and PSV between the two groups before surgery (P>0. 05). It was of no statistically significant difference in ovary sectional area between the two groups after surgery (P>0. 05), but it was of statistically significance in PSV (P<0. 05). Conclusion Compared with electrocoagulation, the laparoscopic excision of ovarian cysts with endoscopic hemostasis had less damage to ovarian, and it could protect the ovarian function.

Objective To analyze the clinical characteristics and nursing measures of infection in hospital ICU,and to provide the refer-ence for clinical nursing. Methods From January 2010 to June 2013,116 patients with nosocomial infection in surgical ICU were collected as observation group,and 72 patients with nosocomial infection in the surgery were as the control group. The risk factors of nosocomial infec-tion were retrospectively analyzed,and the problems related to nursing were discussed. Results The nosocomial infection rate was 8. 44% in ICU,which was significantly higher than the surgical infection rate(4. 43%) in general ward (P<0. 05). Whether patients in ICU or surgery ward,respiratory and urinary tract infections were the most common site of nosocomial infection. The mortality rate in ICU was 9. 48%,which was higher than that of nosocomial infection in surgical ward (4. 17%),P<0. 05. The nosocomial infections were obviously related to the age of patients,duration of hospitalization,the invasive operation,antibiotic and immunosuppressive agents and disturbance of consciousness in ICU and general wards (P<0. 01). Conclusion The ICU nosocomial infection rate was significantly higher than that in general ward,the main infection sites are respiratory tract and urinary tract,with a variety of factors,the key is to establish the mechanism of prevention and ef-fective nursing strategy.

ObjectiveTo explore the dynamic changes of electrocardiography (ECG) in patients with coronary heart disease(CHD) after the percutaneous coronary intervention(PCI). MethodsTo compare and analyze the regularity of the changes of 12-lead ECG on 38 cases in times,including one week,three weeks,one month,two and three months after the PCI. ResultsCompared with the ECG before the operation, the occurrences of the low-lying or the bidirectional T-wave were significantly increased,and lasting to 2 ～ 3 weeks after tie operation(5.0％ and 9.6％va2.6％ ,P ＜ 0.05). The ST segment depression( ＞ 1.0mm) and inverted T-wave in the 3 weeks and 2 months after the operation were significantly increased(5.0％ ,5.7％ vs 2.6％ ,P ＜ 0.05 ; 18.0％ and 25.2％ vs 16.2％ ,P ＜0.01 ). After that,the cases of ST segment depression were reduced to the level before PCI,and there was a significant reduction in the cases of the inverted T-wave ( P ＜ 0.01 ). ConclusionsIn the ECG of the patients with CHD after PCI, there were always early one-off abnormal Q-wave, changes of T-wave (inverted or low-lying, bidirectional)and ST segment depressions,etc. After that ,the changes above would turn back to the level before the operation.

Objective To evaluate performance of the clinical laboratories for detection of TORCH immunoassay. Methods There were 2 times external quality assessment (EQA) for nuclear antibody detection. Each panel consisting of 5 liquid serum samples were distributed. Each participants of the EQA program had to reply the results, the methodological procedure and the kits. All data were analyzed and then provided to all laboratory in our EQA program. Results In 2007, the rate of good response were more than 80% for HSV immunoglobulin-M, Rubella immunoglobulin-M and CMV immunoglobulin-M. Response for Toxo immunoglobulin-M was poor (53.1%). The sensitivities of the commercial kits were quite low (<80%). Some clinical laboratories using the same kits gave quite different S/CO value. Conclusions A lot of clinical laboratories get good score in TORCH detection external quality program. False negative is the major problem.

BACKGROUND:Bone marrow mesenchymal stem cellcontent is less under normal conditions, and easily confounded with other cells. Therefore, to establish a simple feasible in vitro cultured amplification method and to obtain a large number of stable bone marrow mesenchymal stem cells are of important theoretical significance and application value. OBJECTIVE:To establish a simple feasible in vitro culture and amplification method and to obtain numerous stable bone marrow mesenchymal stem cells. METHODS:Bone marrow mesenchymal stem cells were isolated from Sprague-Dawley rats and cultured in vitro using density gradient centrifugation and adherence separation screening. cellmorphological changes were observed under an inverted light microscope. Submicroscopic structure of cells was observed under a transmission electron microscope. Living cellnumber was counted using Trypan blue exclusion method. cellgrowth curve was drawn. cellcycle was analyzed using flow cytometry. The expression of c-kit and CD45 was measured using immunocytochemistry. CD45 expression was analyzed using flow cytometry. RESULTS AND CONCLUSION:(1) celladhesion and pseudopodia could be seen under an inverted light microscope at 24 hours after inoculation;cellcolony formed at 4 days;90%cellconfluence was found at 14 days. After passage, the cells were tended to homogenous and became fibroblast-like cells, which showed whirl-like growing or flamboyancy growing. (2) The size of passage 3 bone marrow mesenchymal stem cells was smal and nucleolus was large, with clear nucleoli under a transmission electron microscope, with the presence of sparse chromatin and low electron density. There was microvil us on the surface of cells, abundant ribosomes could be seen in the cytoplasm with few other cellular organs such as endoplasmic reticulum, mitochondria and Golgi complex. These showed that ultrastructural structure had undifferentiated features. (3) The number of passage 3 bone marrow mesenchymal stem cells was reduced at 1 day after inoculation. The cells began to grow at 2 days, and entered the period of exponential growth at 3 days, entered the flat period at 7 days and the number of cells began to decrease at 9 days. Growth curve exhibited“S”shape. (4) The percentage of S phase cells was 21.1%as detected by flow cytometry after passage 3 bone marrow mesenchymal stem cells were stained by DNA. (5) Immunocytochemistry and flow cytometry had proved that the c-kit positive rate of cells was 53.3%and CD45 positive rate was 1.68%. (6) After osteogenic induction of mesenchymal stem cells for 16 days, cells became oval, and short processes connected each other. The cytoplasm was dark, suggesting that it may be rich in rough endoplasmic reticulum and Golgi complex. They can secrete osteoid. Results verified that obtained bone marrow mesenchymal stem cells stably grew, and actively proliferated.

Objective To investigate the effect of Reduning injection combined with blood purification on vital signs, renal fuction and immune function in elderly patients with multiple organ failure .Methods 76 patients with multiple organ failure were selected in the hospital and randomly divided into two groups.38 patients in control group were treated with actively conventional hematodialysis treatment, and 38 patients in experiment group were treated on the basis of control group with Reduning injection.The changes of vital signs, renal fuction and immune function were compared before and after treatment, and then the efficacy between two groups was compared.ResuIts Compared with control group post-treatment, in experiment group, the blood pressure and 24 h urinary volume were higher, the respiratory rate and heart rate were lower (P<0.05);serum creatinine (Scr), blood urea nitrogen (BUN), and uric acid (UA) were lower (P<0.05);the T lymphocyte cell subsets of CD3 +, CD4 +and CD4 +/CD8 +was higher, the CD8 +T was lower ( P<0.05 ) .ConcIusion Reduning injection combined with blood purification has a good clinical curative effect in treatment of multiple organ failure in elderly patients, and could effectively improve the patient′s vital signs, renal function, regulate immune function, and improve vital signs, which has the vital significance to the clinical therapy.

ObjectiveTo systemically review the effects of timing of initiation of renal replacement therapy (RRT) on prognosis in septic patients with acute kidney injury (AKI).Methods A systematic search for randomized controlled trials (RCTs) and other clinical studies focused on comparing varied timing of initiation of RRT in septic AKI patients was performed in English or Chinese from PubMed, Web of Science, EMBASE, CNKI, Wanfang data, VIP from January 2000 to July 2014. Data screened were extracted with Cochrane systemically review method, and enrolled literature was collected for Meta analysis with RevMan 5.2 software. Total mortality, 28-day mortality, the total length of hospital stay and intensive care unit (ICU) stay in septic AKI patients with early or late initiation of RRT was analyzed. Funnel plots were drawn to detect publication bias.Results Five retrospective comparative studies with a total of 885 patients were enrolled. Random effect model in Meta analysis showed that there was no significant difference in total mortality between early RRT group and late RRT group [65.7% (226/344) vs. 68.7% (239/348), odds ratio (OR) = 0.71, 95% confidence interval (95%CI) = 0.38-1.31,P = 0.27]. The funnel plot demonstrated publication bias. Fixed effect model showed that there was significant difference in 28-day mortality between early RRT group and late RRT group [43.4% (66/152) vs. 55.3% (94/170),OR = 0.59, 95%CI = 0.36-0.94,P= 0.03]. The funnel plot demonstrated publication bias too. The data of total length of hospital stay and ICU stay could not be Meta-analyzed because of different data distribution, but no differences in total length of hospital stay and ICU stay between early and late RRT groups for septic AKI patients were found.ConclusionEarly RRT could reduce the 28-day mortality in patients with septic AKI compared with late RRT, but it did not lower the total mortality.