Objective To establish the quantitative transfusion of hemoglobin method and explore the application of this method in neonatal transfusion.Methods This study selected 93 cases of anemic neonates who had no other underlying diseases from the Neonatal Department of Maternal and Child Health (MCH) in Dalian,and they were the principle of weight and age divided into two groups.The first group (control group:conventional method) was transfused and injected the red blood cells that were leukocytereduced,irradiated and washed twice by 0.15 U/kg under the normal reservation.The study measured the hemoglobin value before transfusion and within 24 h after being transfused,and then detected the hemoglobin,volume,and hematocrit of a small amount of blood during the preparation,and finally calculated the utilization rate of hemoglobin in neonates who were transfused.For the second group (observation group:quantitative erythrocyte injection of hemoglobin),it calculated in reverse how much hemoglobin the children needed to be supplemented,based on the doctor's expected hemoglobin values achieved after children being transfused and the utilization rate of hemoglobin obtained from the first group.Then,according to the calculation,it prepared a small amount of blood of the red blood cells that were leukocyte-reduced,irradiated and washed,made the quantitative injection of hemoglobin twice,and measured the hemoglobin value before transfusion and within 24 h after being transfused.At last,it analyzed statistically the results.Results Both two methods effectively improved the children's anemia (P ＜ 0.05).There was no difference (P ＞ 0.05) for two methods in improving the anemia of children,but the quantitative transfusion of hemoglobin (s =6.6,cv =4.6％) could basically reach the doctor's expected hemoglobin value,and it avoided the situations in the first method (s =14.45,vc =8.6％),like that after transfusion a minority of hemoglobin was too high or it did not reach complete amelioration of anemia of the children.Conclusions Both traditional transfusion method and quantitative transfusion of hemoglobin method can improve the anemia of the children,but the latter one is more suitable for the clinical needs and achieves the true quantitative transfusion.

Using MRS medium, as imitating gastroenteric environments, such as low pH (1.5-4.5) and high bile salt (0.1%~0.4%), the antagonistic properties of Lactobacillus acidophilus were studied. At the same time, the antimicrobial activities on pathogenic E. coli and S. aureus in the intestinal tract and antibiotic tolerance of Lacto-bacillus acidophilus were also studied. The results indicated that Lactobacillus acidophilus survived at pH 2.5 - 4.5 after 6h culture and the living bacterial number could reach 107cfu/mL. One strain even survived at pH 1.5; that living bacterial number were above 106cfu/mL at the 0.1% -0.3% bile salt and a strain survived in 0.4% after 4h culture; that antimicrobial activities to the pathogenic E. coli and 5. aureus were obvious; and that the tolerance to Penicillin and Roxithromycin was medium and power.

Objective To investigate the effect of aluminum on hair loss induc ed by fluoride in fluorosis mice.Methods Sixty male C57BL mice were divided into four groups according to body mass:control group,fluoride (F) group (F-100 mg/L),aluminum(Al) group(Al3+ 270 mg/L) and F + Al group(F-100 mg/L + Al3+270 mg/L).Mice were killed 1 month and 3 months after the experiment,respectively.Bone F content was detected by ion-selective electrode method.The level of bone Al was measured through inductively coupled plasma emission spectrum.Dental fluorosis and hair loss of mice were evaluated by visual method.Results One month after the experiment,no dental fluorosis and hair loss was found in all four groups.The content of bone F was the highest in F group [(2401.649 + 86.835) mg/kg],and the lowest in A1 group [(427.006 + 11.878) mg/kg].The levels of bone F in F + Al group and control group were (1210.332 + 19.531)mg/kg and (538.001 + 33.337)mg/kg,respectively.The difference was statistically significant between any two groups (all P ＜ 0.05).Three month after the experiment,all mice of F treatment group had dental fluorosis and hair loss(10/10).Alopecia areas were found in the neck and back regions only.There was no hair loss in control group,Al group and F + Al group.No dental fluorosis was found in both control and Al groups.Only 2 mice were found with dental fluorosis in F + Al group.The levels of bone F in F group,F + Al group,control group and Al group were (4098.645 + 58.842),(1888.165 ± 12.187),(876.258 + 14.462) and (662.385 ± 8.966) mg/kg,respectively.The difference was statistically significant between any two groups (all P ＜ 0.05).Conclusions The hair loss is found in fluorosis mice.Hair loss of mice is closely associated with the level of F exposure.Al can prevent the occurrence of hair loss induced by F in mice through reducing the accumulation of F.

Diagnosis, Differential ; Ependymoma ; metabolism ; pathology ; Female ; Hemangioblastoma ; metabolism ; pathology ; Humans ; Ki-67 Antigen ; metabolism ; Meningeal Neoplasms ; metabolism ; pathology ; surgery ; Meningioma ; metabolism ; pathology ; surgery ; Middle Aged ; Mucin-1 ; metabolism ; Neoplasm Recurrence, Local ; Vimentin ; metabolism

Objective To discuss the surgical techniques and results of internal fixation supplemented with soft tissue repair of triad injury of the elbow via the combined anterior-lateral approach.Methods A retrospective analysis was done on 15 patients with triad injury of the elbow treated from January 2011 to August 2014.There were 10 males and 5 females,aged 38.4 years (range,20-61 years).Injury resulted from traffic accidents in nine patients,high-level falls in three and groundlevel falls in three.Radial head fractures were Mason type Ⅰ in five patients,type Ⅱ in eight,and type Ⅲ in two.Fractures of the coronoid process of the ulna were Regan-Morrey type Ⅰ in four patients,type Ⅱ in ten and type Ⅲ in one.Time interval between injury and operation was 8.5 d.All patients were firstly operated on through the anterior approach to have Herbert screw fixation or anterior capsule suture lasso fixation of the coronoid fracture as well as Herbert screw fixation of the radial head fracture.And then,elbow lateral incision was made to repair lateral collateral ligament complex and common extensor tendon by 2-0 Ethibon suture.Elbow flexion-extension,forearm pronation-supination and Mayo elbow performance score (MEPS) were used to evaluate the clinical elbow functions.Complications were recorded after operation.Results All patients were followed up for mean 19.6 months (range,12-30 months).All coronoid fractures healed.Radial head fractures in 14 patients healed.Comparison of preoperative to final follow-up variables presented significant differences in elbow flexion-extension [(45.1 ± 5.6) °:(129.5 ± 9.3) °],forearm pronation-supination [(55.4 ±.8.7) °:(140.5 ± 10.3) °] and MEPS [(25.1 ± 9.6) points:(91.2 ± 5.2) points] (P ＜ 0.01).Complications included heterotopic ossification in one patient and nonunion of radial head fracture in one patient.No neurovascular injury of the elbow,elbow residual instability,dislocation,elbow stiffness and active pain occurred.Conclusion Combined anterior-lateral approach with internal fixation and soft tissue repair is a simple and safe method that effectively restore the elbow joint function.

Objective To study the biodistribution of 131 I-2'-deoxy-1-β-D-arabinofuranosy1-5-iodouracil (FIAU) in the rat middle cerebral artery occlusion model and the expression of thymidine kinase (TK) gene in brain tissue after gene-modified stem cell transplantation,and thus evaluate the possibility of further noninvasive monitoring of stem cell transplantation therapy in cerebral infarction.Methods Adenovirus recombinant Ad5-TK-intemal ribosome entry site-brain derived heurotrophic factor-enhanced green florecent protein(IRES-BDNF-EGFP) carrying TK-IRES-BDNF gene was prepared.Cerebral infarction model was established in rats by intraluminal middle cerebral artery occlusion with nylon monofilament.Gene modified bone marrow mesenchymal stem cells were transplanted via intraparenchymal route,lateral ventricle,carotid artery and tail vein,respectively.The normal rats were used as controls.131 I- FAU was prepared to be the tracer for biodistribution study and the ％ ID/g was calculated based on measurement of the tissue radioactivity counts.The expression of TK gene was evaluated by quantitative real-time PCR (QR-PCR) and Western blot analysis.Data were analyzed with independent-samples t-test,one-way analysis of variance (ANOVA) test,and Pearson linear correlation test.Results The ％ ID/g of infarcted brain tissue in the intraparenchymal group was 0.124 ± 0.013,which was significantly higher than that in lateral ventricle group (0.052 ±0.004),carotid artery group (0.061 ±0.002),tail vein group (0.059 ±0.005) and control group (0.005 ±0.001) (t =2.913 - 5.652,all P＜0.05),while there were no statistically significant differences among the other route transplanted groups ( t =0.694 - 1.448,all P ＞ 0.05 ).The differences of ％ ID/g between the infarcted and contralateral sides of brain tissue in all transplanted groups were statistically significant (t =9.004 - 15.734,all P ＜ 0.05 ),while there was no statistically significant difference of this parameter between both sides of brain tissue in control group (t =1.511,P =0.182).The expression of TK gene in intraparenchymal group was significantly higher than other groups (t =7.482 -12.371,all P ＜0.05).The expression levels ofTK gene on QR-PCR showed a positive correlation with ％ID/g of the brain tissue ( r =0.971,P ＜ 0.001 ).Similarly,the ratio of TK/β-actin by the Western blot analysis correlated with the ％ ID/g ( r =0.899,P =0.002 ).Conclusion Intraparenchymal route may be the way of choice for cell transplantation therapy of cerebral infarction.If suitable radionuclide tracer is available,PET or SPECT may be potentially used for noninvasive monitoring of stem cell transplantation in cerebral infarction in vivo.

Objective To observe the effect of low arsenic sub-chronic exposure on blood routine test index in rabbits to pave a way for screening early injury of the low arsenic exposure. Methods Twelve healthy male rabbits were randomly divided into four groups. They were administrated with As at the concentration of 0(control), 10, 50 and 250 μg/L in the drinking water. Blood samples were collected from the vein of the ear edge in 8 weeks, and blood test routine including leukocyte (WBC), lymphocyte (LYM), lymphocyte percentage (LYM%), neutrophil (GRA), neutrophil percentage (GRA%), monocyte (MON), monocyte percentage (MON%), red blood cell (RBC), hemoglobin (HGB), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin content (MCH), mean corpuscular hemoglobin concentration(MCHC), RDW, platelets(PLT), mean platelet volume(MPV), platelet hematocrit(PCT) and platelet distribution width (PDW), were detected by the ABX-60 hemocyte analyzer. Results Compared with the control group, the WBC, GRA and GRA% increased in 0, 50 and 250 μg/L groups, but there was no significance(P > 0.05). PLT and MPV had a statistical significance in 4 groups(F = 4.07,4.20, all P < 0.05). Compared with the control group[(292.00±16.97)×109/L, (7.10±0.99)fL], PLT decreased in the 250 μg/L group [(221.33±22.50)×109/L] and MPV decreased in the 50μg/L group [(5.57±0.46)fL] significantly (P < 0.05). The other index didn't change obviously. Conclusions Sub-chronic low level arsenic exposure may lead to the change in the blood system. The blood routine test may be considered for early injury of the arsenic poisoning.

To compare the clinical efficacy of treating Denis type B thoracolumbar burst fracture with allogenic bone and calcium sulfate implanting in injured vertebra.A total of 46 patients were randomly assigned into 2 groups.Group A( n=22) received allogenic bone implanting in injured vertebra while group B( n=21) had calcium sulfate grafting in injured vertebra.Group A was better than group B in maintaining tanterior vertebral body height and lessening the degree of bone defect ( P <0.05 ).No significant differences existed in operaive duration , blood loss volume, correcting Cobb′s angle, preventing degeneration of adjacent segments , visual analogue scale ( VAS) and Japanese Orthopaedic Association ( JOA) scores and the degree of bone defect ( P>0.05).

Objective To investigate the quality of life and its influence factors in patients with atrial fibrillation 3 months after catheter ablation. Methods From April 2014 to February 2016,AF patients who underwent single catheter ablation in our hospital were included. The quality of life was assessed by MOS 36 item short form health survey (SF-36), and the effect of related factors on QOL scores were further analyzed. Results There were no significant changes in quality of life at baseline and 3 months after catheter ablation including physical component summary (PCS) [(70.6±17.1) vs.(72.9±10.7),P =0.078]score, mental component summary (MCS) [(70.9±12.3) vs.(71.8±12.5), P =0.44] score and SF-36 score [(70.8±13.0) vs. (72.3±10.3), P=0.138]. Multivariate analysis indicated that there was negative correlation between patients who relapsed after ablation and better quality of life(PCS, β=―3.162, P=0.036;MCS, β=―5.675, P =0.001; total SF-36 score, β=―4.385, P =0.003), MCS score and warfarin use (MCS,β=―4.020,P =0.014). There was negative correlation between PCS score and smoking(PCS, β=―3.129,P =0.037).Conclusion All patients had stable quality of life in 3 months after catheter ablation. Ablation outcome was probably the main influencing factors. The use of dabigatran and smoking might also have effects on their quality of life.