Acupuncture Points ; Administration, Cutaneous ; Adult ; Aged ; Drugs, Chinese Herbal ; administration & dosage ; Facial Paralysis ; drug therapy ; Female ; Humans ; Male ; Middle Aged ; Treatment Outcome ; Young Adult

Objective: To evaluate the feasibility of taste-masking by electronic (E)-tongue and provide a reference for the drug taste-masking study. Methods: Andrographis Herba was used as the carrier of bitter Chinese materia medica (CMM), sodium acetate (NaAc), sodium cyclamate (SC), adenosine-5′-monophosphatedisodiumalt (AMP), and 2, 4-dihydroxyhenzoic acid (DA) as the taste-masking agents. The data obtained by the E-tongue were analyzed through principal component analysis (PCA), and Euclidean distance in the data space of different dimensions was calculated. Regarding the distance of bitterness (D) as the evaluation index, then whether the data analyzed by the E-tongue and volunteers were the same or were not investigated. Results: The evaluation results of the taste-masking agents by E-tongue were similar to the taste by volunteers except NaAc, suggesting that some conditions should be required to evaluate the effect of CMM taste-masking. Conclusion: This study shows that E-tongue could be used for evaluating the effect of CMM taste-masking under certain conditions. To evaluate the gustation information by E-tongue, we should use the professional knowledge of pharmacy, chemical and other related disciplines to analyze and improve this evaluation method and model gradually.

Objective To explore the relationship between Mycoplasma pneumoniae infection and bronchial asthma, and provide a theoretical basis for the diagnosis and treatment of bronchial asthma .Methods 54 cases of children with bronchial asthma were selected as the observation group , treatment of children with herpes infection of the upper respiratory tract of 54 cases as the control group .Two groups of children were assayed by eosinophil count , interleukin-13 (IL-13), immunoglobulin IgE (MP-IgM) and Mycoplasma pneumoniae antibody detection .The positive rate of MP-IgM test in two groups were compared .According to the observation group , MP-IgM detection results were divided into MP -IgM positive group and MP -IgM negative group .Positive contrast group , MP-IgM MP-IgM negative group and a control group of children with eosinophil count , IL-13 and IgE were detected .Re-sults The positive rate of MP-IgM in children with observation group detection was 75.93%;The positive rate of MP-IgM in children with control group of detection was 27.78%;The positive rate of MP-IgM detection in chil-dren with the observation group was significantly higher than the control group ( p<0.05); The MP-IgM positive group, MP-IgM negative groups eosinophil count , the levels of IL-13 and IgE levels were significantly higher than that of control group (all p<0.05); In MP-IgM positive group, Eosinophils count (538.18 ±41.63) ×106/L, IL-13 level (253.27 ±40.36) ng/L and IgE level (327.13 ±56.91) kU/L were significantly higher than in MP -IgM negative group (all p<0.05).Conclusion The incidence of asthma is associated with mycoplasma pneumonia infection , and we should strengthen the detection and anti -infection treatment of Mycoplasma pneumoniae in children with asthma .

Resveratrol(RES)is a kind of antitoxin material produced by plant angainst fungi,it has many biological activites, which can restrain a variety of microorganisms.RES was reviewed in this paper with a variety of inhibition microbial func-tions in recent years,and summarized the antibacterial mechanisms of interfering with cell wall protein synthesis,cell mem-brane damage and RsbA mediating antibacterial factor formation.

Objective To investigate the preventive and therapeutic effectiveness of Yisheng herbal injection against chronic allograft nephropathy in rats.Methods Renal transplantation was performed from SD to Wistar closed colony strain rats. Before the transplantation, the kidney was preserved in 0- 40 ℃ heparin sodium chloride solution for one h. The rats were divided into 4 groups: Group A only received CsA 10 mg/kg every day; Groups B, C and D received low dose of Yisheng+CsA, high dose of Yisheng+CsA and CsA+MMF, respectively. Blood creatinine, pathological changes of the renal grafts and the expression of transforming growth factor ?1 in transplanted renal tissues were observed 4, 8 and 12 weeks after operation.Results After the transplantation, the serum creatinine level in group A was significantly higher than in groups B, C and D ( P

Objective To investigate the effect of different immunosuppresants on CAN by affecting TGF-?_ 1 and Smads signal pathway. Methods The SD→Wistar rat accelerated kidney sclerosis model was made following the procedure of Kamada with our modification. The rats were divided into 6 groups (each group n=8): Group A, psudo-OP group; Group B, control group; Group C, CsA 6 mg?kg~ -1?d~ -1; Group D, FK506 0.15 mg?kg~ -1?d~ -1; Group E, MMF 20 mg?kg~ -1?d~ -1; Group F, RPM 0.8 mg?kg~ -1?d~ -1. After 4, 8, 12 weeks, the animals were killed. The immunohistochemistry and real-time fluorescence quantitative PCR were used to detect localization and expression of TGF-?_ 1, Smad2 and smad7 in transplant kidney. Results CsA and FK506 simultaneously stimulated the gene expression and protein production of TGF-?_ 1 and Smad2 and inhibited the expression of Smad7 in transplant kidney, whereas MMF and RPM could down-regulate the gene expression and protein production of TGF-?_ 1 and smad2 and up-regulate the expression of smad7. There was no significant difference between CsA group and FK506 group, as well as MMF group and RPM group.Conclusion Different immunosuppresants can affect TGF-?_ 1 and Smads signal pathway in kidney graft. CsA and FK506 can cause CAN, owning to up-regulate the expression of Smad2, and down-regulate the Smad7 expression. MMF and RPM can prevent the progression of CAN because of down-regulating the expression of Smad2 and increasing Smad7 production.

This study is to investigate the effects of ceramide on GSTA1 expression in Caco-2 cells. After being exposed to ceramide for a fixed time, GSTA1 protein expression was detected by Western blotting analysis; GSTA1 mRNA expression was detected by real time PCR; dual luciferase assay was used to analyze GSTA1 transcriptional activity and GSTA1 activity was determined toward androstanedione (AD) as substrate. The data showed that ceramide can significantly induce the expression of protein and GSTA1 mRNA, and increase transcriptional activity and enzyme activity of GSTA1. The results demonstrated that ceramide may increase resistance to chemotherapeutics in Caco-2 cells by up-regulating the expression of GSTA1.

Objective To study the efficacy of external dissection & internal ligation for beating mixed hemorrhoids, and compare with Milligan-Morgan. Methods One hundred and twenty-six patients with mixed hemorrhoids were divided into two groups: treatment group(66 cases)and controlled group(60cases).In treatment group used external dissection & internal ligation for treating mixed hemorrhoids, but in eontrolled group used Milligan-Morgan. Then the differences between both groups in healing time of wound surface, as well in the statuses of bleeding, pain, edema, anus stenosis, and anus overflow liquid were observed. Results Averaged healing time in treatment group was significantly shorter than that in controlled group, (8.2±2.6)days vs (17.4±3.8)days, P＜0.01.The anus stenosis and anus overflow liquid were significantly less in treatment group than those in controlled group, P＜0.01.Conclusion External dissection & internal ligation of hemorrhoids for treating mixed hemorrhoids decreases anal transitional zone(ATZ)disorganization, shorten healing time, and significantly decreases crissum scar,protectes anus function effectively.

Humans ; Maxillary Sinus ; anatomy & histology ; surgery

Objective: To explore the feasibility of the application of small specimens as alternatives of gross specimens to detect EGFR (epidermal growth factor receptor) mutation in NSCLC (non-small cell lung cancer) by ARMS (amplification refractory mutation system). Methods: Biopsy specimens from 181 cases of NSCLC were collected, in which 157 were small specimens (including specimens acquired through CT-guided percutaneous lung biopsy, endobronchial ultrasound-guided transbronchial needle aspiration, lymph node biopsy, bronchoscopic biopsy and aspiration of pleural effusion) and 24 were gross specimens. QIAGEN DNA extraction kit was used to extract DNAs from NSCLC specimens. Then AmoyDx EGFR Mutation Test Kit - a highly sensitive real-time PCR-based test was used to detect EGFR mutations. The detection rates of EGFR mutations in small specimens and gross specimens were compared by Chi-square test or Fisher's exact test. Results: The total EGFR mutation detection rate of all biopsy specimens was 39.8% (72/181). The detection rates of small specimens and gross specimens were 38.9% and 45.8%, respectively (P = 0.515). Furthermore, EGFR mutation frequencies were significantly higher in non-smokers (P = 0.033) and in patients with adenocarcinoma (P < 0.001). Conclusion: A relatively high detection rate of EGFR mutation in NSCLC small specimens can be obtained through ARMS. For patients with advanced NSCLC whose gross specimens cannot be easily obtained, the alternative small specimens can be used in clinical EGFR mutation detection. Copyright © 2012 by TUMOR.