Objective To investigate the effects of erythropoietin(EPO) on extracellular matrix remodeling and the expressions of matrix metalloproteinase(MMPs) and tissue inhibitor of metalloproteinase-4(TIMP-4) after myocardial infarction in rats.Methods Eighty male SD rats were randomly divided into four groups:sham operation(SH) group,sham operation and drug(SHE) group,myocardial infarction(MI) group,and myocardial inferct and erythropoietin(MIE) group.Myocardial infarction in MI group and MIE group was reproduced by ligation of a branch of left anterior descending coronary artery.Hematocrit,echocardiography,capillary density,infarct area,fibrosis and collagen volume fraction were tested,and MMP-2,MMP-9 and TIMP-4 were measured by immunohistochemistry at 1-week and 6-week after myocardial infraction.Results One week after myocardial infraction,the protein expressions of MMP-2 and MMP-9 in MI and MIE groups were significantly higher than that in SH and SHE group(P0.05).Six weeks after myocardial infraction,echocardiography revealed that the left ventricular end-diastolic dimension(LVDd),left ventricular end-systolic dimension(LVDs),left ventricular end-diastolic volume(LVEDV) and left ventricular end-systolic volume(LVESV) were significantly increased,and left ventricular ejection fraction(LVEF) and left ventricular fractional shortening(LVFS) were significantly decreased in MI and MIE groups compared with those in SH and SHE groups(P

Chemical synthetic drugs are the main therapeutic drugs for cancer, however, the time-consuming drug development, lengthy treatment, serious side effects and drug resistant of chemical synthetic drugs significantly increase the burden of the patients.In recent years, natural anti tumor substances with good treatment effectiveness and low toxicity, have attracted more and more attentions in studying anti tumor drugs.It is reported that Inonotus obliquus can significantly suppress tumors, while the antitumor components and mechanisms of Inonotus obliquus are rarely reported.In this paper, we will review the antitumor effect, antitumor components and mechanisms of Inonotus obliquus.

Two inequalities in the book Navier-Stokes Equations Theory and Numerical Analysis written by Roger Temam are improved via Schwarz's inequality and Young's inequality, and the coefficients of them are simplified from 21/4·31/2 and 21/2·33/4 to 21/4 and 23/4, respectively. Therefore, to some extent the approximating error can be reduced and the accuracy of approximation can be improved.

Objective To observe the effect of Tongxinluo capsule combined with Jinlidas intervention on vascular endothelial fuction and blood glucose in the patients with Type 2 diabetes. Methods A total of 100 patients with Type 2 diabetes were randomly divided into treatment and control groups with 50 patients each. Both groups were received subcutaneous injection insulin, oral admistration of Simvastatin and Aspirin. And the treatment group were received Tongxinluo capsule and Jinlida sintervention based on the standard treatment. The changes of serum levels of FPG, 2hPG, HbAlc, VEGF, NO, ET-1 were observed before and after treatement. All the patients were treated for 12 weeks. Results After the treatment, the serum of FPG (7.03 ± 2.11 mmol/L vs. 8.09 ± 2.03 mmol/L, t=1.911), 2hPG (9.27 ± 2.03 mmol/L vs. 12.06 ± 3.54 mmol/L, t=1.946), HbAlc (6.98%± 1.58%vs. 8.06% ± 2.13%, t=2.013) were decreasd (P<0.05). Besides, VEGF (684.61 ± 84.37 ng/L vs. 514.28 ± 79.52 ng/L, t=2.136), NO (43.28 ± 6.40 ng/L vs. 34.64 ± 6.70 ng/L, t=2.097) of treatment group were siginificantly higher than the control group (P<0.05), while ET-1 (53.13 ± 7.24μmol/L vs. 60.09 ± 7.18μmol/L, t=2.271) were siginificantly lower (P<0.05). Conclusion Tongxinluo capsule combined with Jinlida sintervention could decreased the level Blood glucose and improve vascular endothelial fuction in the patients with Type 2 diabetes.

Objective To explore correlation between gene polymorphism of TNF-alpha and thyroid associated ophtalmopa thy (TAO) in Zhuang ethnic population.Methods DNA and serum samples were collected in patients with TAO in Han/Zhuang population.ELISA was applied to detect level of TNF-alpha in serum and PCR was applied to detect polymorphism of TNF-alpha gene 863C_A.Results The levels of TNF-alpha in patients with TAO increased significantly compared with control group (P ＜ 0.05),and more significantly in Zhuang population compared with Han population (P ＜ 0.05) ;gene frequency of CA was significantly higher in patients with TAO in Zhuang population than that in Han population (P ＜ 0.05),while gene frequency of AA was significantly lower (x2 =0.770,2.145,all P ＜ 0.05).Conclusion Serum level of TNF-alpha increases during occurrence of TAO,and the differences of expression level between different nationalities are due to gene polymorphism of TNF-alpha.

Animals ; Humans ; Reproducibility of Results ; Selenium ; analysis ; Spectrometry, Fluorescence ; Spectrophotometry, Atomic

Objective To design the meta-data scheme for medical records database of traditional Chinese medi-cine.Methods The meta-data scheme for medical records database of traditional Chinese medicine was designed u-sing the MARC meta-data according to the analysis of resources, investigation of literature, networks and users. Results The meta-data scheme for medical records database of traditional Chinese medicine is consisted of 17 fields and 9 parts.Conclusion MARC, as the mata-data criteria, can organize and describe the medical records of tradi-tional Chinese medicine.However, the standards for selection and description of meta-data criteria need to be fur-ther studied.

Objective To explore the influence and curative effect of leflunomide combined with ginkgo leaf extract and dipyridamole on hypercoagulable state in patients with nephrotic syndrome.Methods Ninety-five patients who were the first diagnosed nephrotic syndrome were divided into experiment group (50 cases) and control group (45 cases) by random digits table method.The patients in experiment group were treated with leflunomide combined with ginkgo leaf extract and dipyridamole,while the patients in control group were treated with leflunomide.The coagulation function and renal function indexes were respectively detected before and after treatment.Results After treatment,the expression level of platelet activation complexes (PAC)-1 in experiment group decreased from (7.33 ± 1.69)％ to (2.58 ±0.97)％,and the expression level of CD62p decreased from (6.15 ± 1.59)％ to (3.18 ± 1.03)％,which were significantly lower than those after treatment in control group [(7.23 ± 1.41)％ and (5.99 ± 1.51)％],and there were statistical differences (P ＜ 0.05).The prothrombin time in experiment group extended from (10.62 ±0.45) s to (12.88 ± 0.69) s,and the activated partial thromboplastin time extended from (23.33 ± 2.79) s to (30.57 ±3.88) s,which were significantly longer than those after treatment in control group [(11.21 ±0.48) s and (23.46 ± 2.79) s],and there were statistical differences (P ＜ 0.05).The plasma D-dimer in experiment group decreased from (2.58 ± 1.23) mg/L to (0.89 ± 1.12) mg/L,the plasma fibrinogen decreased from (3.68 ± 1.21) g/L to (1.89 ± 0.74) g/L,which were significantly lower than those after treatment in control group [(2.33 ± 1.22) mg/L and (3.25 ± 1.33) g/L],and there were statistical differences (P＜0.05).The serum creatinine,blood urea nitrogen and 24 h urine protein levels after treatment in the 2 groups were significantly lower than those before treatment,but the serum creatinine and 24 h urine protein levels after treatment in experiment group were significantly lower than those in control group [(85.38 ± 18.56) μ mol/L vs.(115.23 ± 20.88) μ mol/L,(1.07 ± 0.38) g vs.(2.57 ± 0.99) g],and there were statistical differences (P ＜0.05).Conclusions The application of the leflunomide combined with ginkgo leaf extract and dipyridamole has great effects on releasing the hypercoagulable state and improving the renal function of patients with nephrotic syndrome.

Pseudorabies is an economically important disease in a variety ot animals caused by pseudorabies virus. Since 2011, pseudorabies outbreaks occurred in many regions of China. Related researches on this virus become a hot topic in virology and veterinary. One of the difficulties for pseudorabies prevention and control is innate immune evasion. Explorations on this issue are conducive to the development of vaccine and drugs. Therefore, this review summarized the recent research progress on the mechanisms of pseudorabies virus innate immune evasion. Theoretical direction was provided on effetive prevention and control of pseudorabies owing to this review.
Animals ; Herpesvirus 1, Suid ; genetics ; immunology ; Humans ; Immune Evasion ; Immunity, Innate ; Pseudorabies ; immunology ; virology

Objective To investigate the clinical and pathological features of Henoch-Sch?nlein purpura nephritis (HSPN) in children. Methods The clinical and pathological data of 242 children with HSPN admitted from January 2009 to January 2014 were retrospectively analyzed. Results The most common clinical type was proteinuria and hematuria (135 cases, 55.79%). The mainly pathological types were typeⅡandⅢ. The distribution of pathological types was statistically signiifcant between different clinical types (P=0.000). The patients with normal urine test had the lowest pathological grade. The patients with isolated hematuria and proteinuria mainly had pathological typeⅡ. The patients with hematuria and proteinuria mainly had pathological typeⅢa. The patients with nephrotic syndrome mainly had pathological typeⅢb. The length of the courses at renal biopsy was not statistically signiifcant among different clinical types (P>0.05) and pathological types (P>0.05). The deposition of immune complex in kidney was not statistically signiifcant among different pathological types (P>0.05). The levels of IgA, C3, and platelet count were not statistically signiifcant among different clinical and pathological types (P>0.05). Conclusion The clin-ical classiifcation is related to the pathological grade in children with HSPN.