To treat 30 cases of diarrhea of insufficiency of the spleen-yang type by moxibustion with salt at Shenque (CV 8 ). The total effective rate was 93.3%.

BACKGROUND: Intestinal mucosa was damaged by ischemia and hypoxia during severe burn, and injury and infection of oxygen-derived free radicals during reperfusion.OBJECTIVE: To study on the basis of sub-cell level whether danshen can improve respiratory function of mitochondrion of epithelial cell in intestinal mucosa and decrease the production of oxygen-derived free radicals so as to protect intestinal mucosa of burnt rats.DESIGN: Randomized controlled animal study with single sample.SETTING: Department of Burn, the Third Hospital of Chinese PLA.MATERIALS: The experiment was conducted at the Surgery Institute of the Third Hospital of Chinese PLA from December 2001 to February 2002.Totally 96 SD rats of clean grade were randomly divided into normal control group (n=24), burning model group (n=36) and danshen treatment group (n=36). 1 mL danshen parenteral solution had 1.5 g raw materials.METHODS: ① Rats in the burning model group and danshen treatment group were used to establish grade Ⅲ burning models with 20% scald of total body surface. ② Rats in the normal control group were not injured.③ After modeling, 1 mL/kg danshen parenteral solution was slowing injected into rats in the danshen treatment group through femoral vein, but saline was injected slowly into rats in the normal control group and burning model group. ④ Twelve rats from each of the burning mgdel group and the danshen treatment group were sacrificed at 1, 2 and 6 hours after modeling, and 8 rats in the normal control group were sacrificed at relevant time points respectively. ⑤ Samples of small intestine were collected to measure cytochrome aa3, cytochrome C, level of energy charge and activity of superoxide dismutase of mitochondrion of epithelial cells in intestinal mucosa.MAIN OUTCOME MEASURES: Cytochrome aa3, cytochrome C, level of energy charge and activity of superoxide dismutase of mitochondrion of epithelial cells in intestinal mucosa 1, 2 and 6 hours after modeling.RESULTS: Totally 96 SD rats entered the final analysis. ① Level of cytochrome aa3 of mitochondrion of epithelial cells in intestinal mucosa at various time points after modeling: Levels of cytochrome aa3 did not changed obviously in the burning model group and danshen treatment group 2 hours after modeling as compared with that in the normal control group (P＞ 0.05). Six hours after burning, levels in the burning model group were obviously lower than those in the normal control group (P ＜ 0.05), but those in the danshen treatment group were obviously higher than those in the burning model group [(3.16±0.13), (2.5640.15) μkat/g, P ＜ 0.05]. ②Measurements of cytochrome C, level of energy charge and activity of superoxide dismutase of mitochondrion of epithelial cells in intestinal mucosa at various time points after modeling: As compared with those in the normal control group, measurements were decreased obviously in the burning model group 1, 2 and 6 hours after modeling (P ＜ 0.05 or 0.01), but those in the danshen treatment group were obviously higher than those in the burning model group (P ＜ 0.05 or 0.01).CONCLUSION: Danshen can raise the levels of cytochrome aa3, cytochrome C, energy production and SOD, and also reduce the production of oxygen-derived free radicals so as to improve respiratory function of mitochondria of epithelial cells in intestinal mucosa of rats.

Drug Combinations ; Drug Design ; Drugs, Chinese Herbal ; pharmacology ; Drugs, Investigational ; Humans

Objective To investigate the expression and significance of osteopontin (OPN) and monocyte chemoattractant protein-1 (MCP-1) in lipid -induced rat renal injury. Method Wistar rats were randomly divided into two groups: normal control rats (C group) and cholesterol fed rats (H group). Twelve weeks later, the 24h total urine protein amount, creatinine clearance rate and the levels of total serum cholesterol, LDL- cholesterol, HDL- cholesterol and triglycerides were measured. Kidney pathological changes were observed by HE and PAS statning. Immunohistochemitry were used to detect the expression of OPN, MCP-1 and ED1. Results Total serum cholesterol level, LDL-cholesterol level and 24h total urine protein amount in H group rats were higher than those in C group rats, and there were no significant difference between two groups in HDL-cholesterol and triglycerides level. Compared with C group rats, the expression of OPN and MCP-1 increased in cortical tubular epithelium of H group rats, and the number of ED1 + cells increased in tubulointerstitium as well. Either OPN or MCP-1 expression was positive correlation with the number of ED1 + cells. Conclusion The expression of OPN and MCP-1 in tubular epithelium increased in hypercholesterolemia rats, which might mediate M? infiltration into tubulointerstitium and lead to interstitial fibrosis.

Bioceramics, because of their advantages such as biocompatibility and osteoconduction, have been widely used for bone repairing and bone substitute as bone tissue engineering materials. This study primarily compares the characteristics and the application range of some kinds of bioactive ceramics and biodegradable ceramics, and introduces their latest development in degradability and strength et al. Among these materials, calcium polyphosphate is a new material as bone tissue engineering scaffold. With a controllable degradation rate and the required strength, it has been paid much attention.

Adenoma ; metabolism ; pathology ; surgery ; Aged ; Diagnosis, Differential ; Endodermal Sinus Tumor ; pathology ; Female ; Humans ; Immunohistochemistry ; Neprilysin ; metabolism ; Ovarian Neoplasms ; metabolism ; pathology ; surgery ; Sex Cord-Gonadal Stromal Tumors ; pathology ; Stromal Cells ; metabolism ; pathology ; Vimentin ; metabolism

OBJECTIVETo compare the difference in the clinical efficacy on perennial allergic rhinitis between three nasal points acupuncture therapy and the oral administration of loratadine so as to provide the better acupuncture program in clinical treatment.

METHODSSixty cases were randomized into an acupuncture group (30 cases) and a medication group (30 cases). In the acupuncture group, acupuncture was applied to three nasal points [Yingxiang (LI 20), Yintang (EX-HN 3), Bitong (Extra)] and acupoints selected by syndrome differentiation. Acupuncture was given once every two days, three times a week, for 4 weeks totally. In the medication group, loratadine was prescribed for oral administration, 10 mg every day, for 4 weeks. The symptom and physical sign scores before and after treatment, as well the short-term and long-term efficacy were compared between the two groups.

RESULTSThe total effective rate was 96.7% (29/30) in the acupuncture group and was 93.3% (28/30) in the medication group after treatment. The efficacy was similar between the two groups (P>0.05). In follow-up, the total effective rate was 86.7% (26/30) in the acupuncture group, which was better than 56.7% (17/30, P<0.05) in the medication group. The scores of symptoms and physical signs after treatment and in follow-up were all reduced apparently as compared with those before treatment in the patients of the two groups (all P<0.05). The scores of symptoms and physical signs were reduced more apparently in the acupuncture group as compared with those in the medication group in follow-up (all P<0.05).

CONCLUSIONThe acupuncture at three nasal points and the acupoints selected by syndrome differentiation achieves the similar short-term efficacy on perennial allergic rhinitis as compared with the oral administration of loratadine. The acupuncture therapy presents the obvious advantages on long-term efficacy.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Female ; Humans ; Male ; Middle Aged ; Nose ; Rhinitis, Allergic, Perennial ; therapy ; Young Adult

Endophthalmitis ; diagnosis ; drug therapy ; etiology ; Eye Infections, Fungal ; diagnosis ; drug therapy ; etiology ; Humans ; Infant, Newborn ; Infant, Premature ; Male

?ln recent years, the number of patients with diabetes increase rapidly. Diabetic retinopathy ( DR ) , one of the complications of diabetes, is also the important aspect of current and future prevention of blindness in our country. Now, more and more scholar have noticed the important role of immune inflammation in the pathogenesis of diabetic retinopathy. ln this article, we reviewed the role of interleukin-6 ( lL-6 ) in diabetic retinopathy.

Objective To assess the renal protective effects of triptolide treatment in type 2 diabetic rats and its possible mechanisms.Methods Wistar rats were randomized to receive following approach control (n=7),dia- betic model without treatment (n=7) and diabetic group treated with triptolide[200 ?g/(kg?d),n=7].Plasma glucose (PG),serum creatinine (Scr),total cholesterin (TC),triglyeride (TG),kidney mass (KM),index number of kidney hypertrophy (KM/body mass,KM/BM),and 24 hours urinary albumin (24 h UAL) were determined. The protein expression of monocyte chemoattractant protein-1 (MCP-1),osteopontin (OPN) and ED-1 in renal tissue were determined by immunohistochemical technique.The mRNA expressions of MCP-1 and OPN in kidney tissue were assessed by reverse transcription-polymerase chain reaction (RT-PCR).Results Elevated 24 h UAL was markedly attenuated by triptolide treatment [24 h UAL,triptolide:2.32?0.29 vs diabetic model group:3.89? 0.51 mg/mgCr,P