1.Clinical research progress of straight leg raising test
Journal of Chinese Physician 2017;19(7):1119-1120,封3
As a clinical diagnosis and treatment of low back pain,straight leg raising test has been used most commonly more than 100 years.It is simple and practical,and has a high clinical value.As a very mature orthopedic special examination,straight leg raising test progress in non-orthopedic applications more often than orthopedic aspects.Of course,the most important thing is the diagnosis of low back pain,followed by the treatment of low back pain,and then can be used as a respiratory and circulatory aspect of diagnosis and treatment options.This article elaborated the principle of straight leg raising test and the clinical research progress in order to give the clinician some guidance.
2.Effect of acceptance and commitment therapy on postoperative psychosocial adaptation in breast ;cancer patients
Rui JIAO ; Weilian JIANG ; Yingdong HU
Chinese Journal of Practical Nursing 2016;32(34):2662-2665
Objective To investigate the effects of acceptance and commitment therapy on postoperative psychosocial adaptation in breast cancer patients. Methods 120 breast cancer patients were divided into two groups by random number table:58 cases in the observation group and 62 cases in the control group. They all received routine nursing care and the observation group also received acceptance and commitment therapy intervention, including acceptance, cognitive diffusion,being present, self as context, values and committed action. Psychological and Social Adaptation Questionnaire for Breast Cancer Patient (PSAQ-B) was used to investigate their psychosocial adaptation before and after the intervention. Results The intervention group was observed in patients with anxiety/depression, self-esteem and self-acceptance, attitude, sense of belonging, self-control and self-efficacy score was respectively (30.14 ± 5.08), (31.68 ± 5.77), (30.03 ± 5.35), (33.68 ± 5.56), (32.86 ± 5.63) points, the control group were (23.75 ± 3.68), (28.87±4.86), (26.12 ± 4.53), (29.18 ± 5.26), (29.06 ± 4.75) points, the difference between 2 groups was statistically significant (t = 6.016- 6.540, all P < 0.05). Conclusions Acceptance and commitment therapy can effectively improve the psychosocial adaptation status in breast cancer patients after surgery to help improve their quality of life.
3.Establishment of experimental atherosclerosis model in rats
Pengyuan YANG ; Yaocheng RUI ; Yabin JIAO ;
Academic Journal of Second Military Medical University 2000;0(07):-
Objective: To establish an experimental model of atherosclerosis in rats and to study the atherosclerotic lesions in the aorta,heart and liver.Methods: The rats in experimental groups were injected with a single dose of vitamin D (600 000 IU/kg) and loaded with high fat diet;control group was given saline and basic food.The pathological changes were observed in the aorta,heart and liver after 2,4,6 weeks.The scores were evaluated according to the pathological degrees.Results: No changes were observed after 2 weeks,but after 4 weeks atheroslerotic plaques were seen in the aorta and the score of the lesions were 0.50?0.39; a little lipid were found in coronary arteries;deposition of lipid was seen in the myocardium; many positive red pellets were found in the plasma of cells in the liver.After 6 weeks,more atheroslerotic plaques were observed in the aorta,and atheroslerotic plaques in coronary arteries were formed (pathological score 1.12?0.48); abundant positive red pellets were found in the plasma of the cardiac myocyte.The pathological changes occurred in rats were very similar to that of man.Conclusion: The experimental model of atherosclerosis in rat may be conveniently established by injection of vitamin D with loading of high fat diet,which can be used for the pathological and pharmacological study of atherosclerosis.
5.Analysis of the volatile oil in fructus lycii(Lycium barbarum L.) by GC-MS and HELP method
Boqian LI ; Jiao XU ; Jianmei GU ; Rui YANG ; Lijuan CHEN
International Journal of Traditional Chinese Medicine 2017;39(4):347-350
Objective Gas chromatography-mass spectrometer (GC-MS) technology combined with Heuristic evolving latent projections (HELP) method were used to qualitatively analyze the volatile oil of fructus lycii (Lycium barbarumL.)Methods With the best temperature-programmed chromatographic condition, overlapping peaks among the total ion chromatogram were separated. Then an automatic mass spectral deconvolution and identification system (AMDIS) was used to identify volatile components comprehensively. Finally, the pure chromatographic peaks were matched with NIST 05a mass spectra library for giving the precise qualitative result. Results Totally, 29 compounds in fructus lycii (Lycium barbarum L.) were identified accurately.Conclusions Compared with the traditional qualitative analysis method, the new established method which GC-MS technology combined with HELP could get tremendous advantages. It not merely enhanced the accuracy of identification but also solved the bottleneck of handling the inseverable compounds.
6. Effects of bisphenol A exposure during embryonic phase on development and neurobehavior of zebrafish
Journal of Shanghai Jiaotong University(Medical Science) 2019;39(5):458-462
Objective • To investigate the effects of bisphenol A (BPA) on the development and neurobehavior of zebrafish. Methods • The zebrafish fertilized eggs that developed to 4 hpf (hours post-fertilization) were treated with different concentrations of BPA (0, 2.5, 25, 250, and 2 500 μg/L). Survival rates, malformation rates, hatching rates, 24 hpf autonomic movement, and 48 hpf heart rates in the groups were observed and recorded. And the neurobehavioral test of larval zebrafish that developed to 144 hpf was performed. Results • The BPA exposure did not affect the survival rates, malformation rates and hatching rates of zebrafish. Compared with the control group, the autonomic movement in 24 hpf and heart rate in 48 hpf of zebrafish embryos that exposed to 2 500 μg/L BPA decreased (P=0.000), and the total distance and average speed of larval zebrafish that developed to 144 hpf also decreased (P=0.000). The behavior of larval zebrafish exposed to 250 μg/L and 2 500 μg/L BPA changed on light-evoked startle escape response activity, mainly including the decrease of total distance under strong light stimulation (P<0.05). Conclusion • BPA exposure during embryonic phase has developmental toxicity and neurotoxicity on zebrafish.
7. Significance of EphA2 receptor expression in human gliomas
Tumor 2007;27(8):646-650
Objective: To investigate the expression and significance of erythropoietin-producing hepatocellular A2 (EphA2), a receptor of tyrosine kinase in human gliomas and the correlation between EphA2 expression and tumor angiogenesis. Methods: Immunohistochemistry was used to examine the EphA2 expression in 66 surgically resected human glioma tissues, 2 human glioblastoma multiforme (GBM) cell lines(U251 and U87) and 10 normal brain tissues. Next, we performed Western blotting to further investigate the expression of EphA2 in high-grade GBM and normal brain tissues. Microvessels in the glioma tissues were counted after immunostaining for CD34, and the c orrelation between EphA2 expression and microvessel counts (MVCs) in glioma tissues were assessed. Results: Our immunohistochemical analyses demonstrated variable levels of EphA2 expression in 63 of 66 cases examined (63/66, 95.5%). But EphA2 expression was not detected in all 10 normal brain samples and 3 cases of low-grade gliomas (I-II). There was statistical difference in EphA2 expression between human gliomas and normal brain samples (P < 0.01). The positive rate of EphA2 expression was significantly higher in high-grade gliomas (WHO III-IV) than in low-grade gliomas (WHO I-II) (P < 0.01). The specific EphA2 staining was observed in both glioma tissues and U251 cells. The expression level of EphA2 protein was significantly lower in U87 cells than that in U251 cells. It was not detected in normal brain tissues. Microvessel density (MVD) significantly correlated with expression level of EphA2 protein (P <0.01). The MVD was higher in EphA2-strong positive tumor areas. Conclusion: EphA2 is specifically over-expressed in high-grade gliomas, but is not detected in normal brain tissues. EphA2 may be a novel marker of malignant glioma. It can be used as a molecular target for therapeutic intervention against high-grade glioma. EphA2 is involved in angiogenesis and plays an important role in the initiation and progression of glioma.
8.The study on antibacterial activity in vitro of Cefdinir and other four antimicrobial agents against pathogen from community respiratory tract infections
Jiao QIAN ; Dong CHAI ; Zhuanjie ZHENG ; Rui WANG ;
Chinese Journal of Laboratory Medicine 2001;0(03):-
Objective To evaluate the antibacterial activity of Cefdinir compared with other four antimicrobial agents against common isolates in vitro.Methods Minimal lnhibitory Concentrations (MIC) were determined by agar dilution method Results Staphylococcus aureus (MSSA) showed highest susceptibility to Cefdinir with 100% susceptible rate, Streptococcus pneumoniae (including PSSP and PISP) showed higher susceptibility to Cefdinir with 63 6% 100 0% susceptible rate Hemophilus influenza and Moraxelle catarrhalis both showed good susceptibility to three kinds of cephalosporins and Azithromycin Klebsiella pneumoniae (including ESBLs positive strains) were resistant to three kinds of cephalosporins, while ESBLs negative Klebsiella pneumoniae were sensitive to three kinds of cephalosporins, with 73 10%~88 50% susceptible rate Conclusion Compared with other four antimicrobial agents, Cefdinir has a powerful antibacterial activity to pathogen from respiratory tract infections
9.An evaluation of three electronic apex locators on detecting root perforations in vitro
Yueheng LI ; Zhi ZHOU ; Jun LUO ; Rui LI ; Jiao CHEN
Journal of Practical Stomatology 1995;0(04):-
0.05).Conclusion: For teeth with perforation in root canal therapy,the Propex,Raypex 5 and Root ZX electronic apex locators can detect the perforation with high accuracy.
10.Influence of Yes-associated protein-1 on proliferation and metastasis of gallbladder carcinoma
Rui HE ; Yang WANG ; Yingbin HUANG ; Jun DU ; Xingyuan JIAO
Chinese Journal of Hepatobiliary Surgery 2017;23(2):95-99
Objective To investigate the expressions of Yes-associated protein-1 (YAP1) in gallbladder mucosal epithelium of normal persons,in patients with simple/calculous cholecystitis,and in patients with gallbladder carcinoma;and to study the mechanism of YAP1 in gallbladder carcinoma development.Methods Immunohistochemistry was used to detect the expression and distribution of YAP1 protein in 50 persons with normal gallbladder,101 patients with simple cholecystitis/calculous cholecystitis and 100 patients with gallbladder carcinoma.RT-PCR and western-blot were used to detect the mRNA and protein levels of YAP1 in normal and malignant gallbladder mucosal epithelium cells.siRNA was used to shut down the expression of YAP1 in SGC996 cells.MTT was used to test cell vitality.Flow cytometry was used to measure cell cycle.Results Immunohistochemistry revealed the expression rates of YAP1 in the gallbladder carcinoma group,the cholecystitis/gallstone group and the control group to be 87.0% (87/100),56.4% (57/101) and 5.0% (1/20),respectively (P < 0.01).The YAP1 protein levels were higher in gallbladder carcinoma tissues and cells when compared to normal tissues and cells.RT-PCR showed the mRNA levels of gallbladder carcinoma cells to be 12.5 ± 1.2 times of normal gallbladder mucosal epithelial cells (P < 0.05).After using siRNA to shut down the YAP1 expression,EMT associated proteins were down-regulated,cell vitality was decreased,and cell cycle was arrested in the S-phase.Conclusions YAP1 is closely related to cell proliferation and metastasis of gallbladder carcinoma.It may promote tumor progression through epithelial-mesenchymal transition.transition;Tumor progress